International Immunopharmacology, Journal Year: 2023, Volume and Issue: 120, P. 110290 - 110290
Published: May 20, 2023
Language: Английский
Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 188, P. 106546 - 106546
Published: Jan. 25, 2024
Language: Английский
Citations
8
Journal of Proteome Research, Journal Year: 2020, Volume and Issue: 19(8), P. 3109 - 3122
Published: June 22, 2020
The decline of clinically effective antibiotics has made it necessary to develop more antimicrobial agents, especially for refractory biofilm-related infections. Silver nanoparticles (AgNPs) are a new type agent that can eradicate biofilms and reduce bacterial resistance, but its anti-biofilm mechanism not been elucidated. In this study, we investigated the molecular AgNPs against multidrug-resistant Pseudomonas aeruginosa by means tests, scanning electron microscopy (SEM), tandem mass tag (TMT)-labeled quantitative proteomics. results tests demonstrated inhibited formation P. biofilm disrupted preformed biofilm. SEM showed when exposed AgNPs, structure was destroyed, along with significant reduction biomass. TMT-labeled proteomic analysis revealed could defeat in multiple ways inhibiting adhesion motility, stimulating strong oxidative stress response, destroying iron homeostasis, blocking aerobic anaerobic respiration, affecting quorum sensing systems. Our findings offer insight into clarifying biofilms, thus providing theoretical basis clinical application.
Language: Английский
Citations
64
Frontiers in Cellular and Infection Microbiology, Journal Year: 2021, Volume and Issue: 11
Published: April 1, 2021
Biogenic silver nanoparticles (bio-AgNPs) are increasingly recognized as an antibiofilm and antivirulence strategy against P. aeruginosa , a bacterium that causes chronic infections in immunocompromised cystic fibrosis patients. This study aimed to investigate the effects of subinhibitory concentrations bio-AgNPs on motility biofilm formation . Bio-AgNPs were synthesized via reduction ionic catalyzed by cell-free culture filtrate from Fusarium oxysporum A total 17 isolates strains evaluated for swarming, swimming, twitching presence absence (control) bio-AgNPs, including 10 clinical patients with without fibrosis, 5 environmental obtained public water supply system, 2 reference (PAO1 PA14). Isolates identified biochemical molecular methods. Minimum inhibitory (MICs) determined broth microdilution method. Swarming, assays performed Petri dishes. Biofilm capacity was assessed quantitatively crystal violet MIC values ranged 15.62 62.50 µM. The results showed (½ MIC, 7.81–31.25 µM) significantly increased (p < 0.05) 40.0, 46.7% isolates, respectively. Subinhibitory bio-AgNP treatment enhanced PA14 isolate (P11). It is concluded some isolates. These virulence factors directly involved quorum-sensing systems. Further research should AgNPs quorum sensing help elucidate their mechanism action at concentrations.
Language: Английский
Citations
48
Journal of Medicinal Chemistry, Journal Year: 2021, Volume and Issue: 65(1), P. 688 - 709
Published: Dec. 24, 2021
Development of new bacterial biofilm inhibitors as antibacterial synergists is an effective strategy to solve the resistance Pseudomonas aeruginosa. In this paper, a series 3-hydroxy-pyridin-4(1H)-ones were synthesized and evaluated, hit compound (20p) was identified with effects inhibiting production pyocyanin (IC50 = 8.6 μM) formation 4.5 μM). Mechanistic studies confirmed that 20p inhibits by expression pqsA, blocking pqs quorum sensing system quinolone biosynthesis. Moreover, we systematically investigated bactericidal combining currently approved antibiotics for CF including tobramycin, ciprofloxacin, colistin E 20p, which showed obvious synergy overcome in multidrug-resistant P. aeruginosa biofilms. The result indicates may be used future potentially novel synergist candidate treatment infections.
Language: Английский
Citations
41
Antibiotics, Journal Year: 2022, Volume and Issue: 11(11), P. 1524 - 1524
Published: Nov. 1, 2022
The rapid emergence of antimicrobial resistance (AMR) among bacterial pathogens results in treatment failure and the high mortality rate associated with AMR. application nanoparticles synthesized from probiotics will be widely accepted due to their efficacy biocompatibility treating microbial infections humans. current work sought isolate identify lactic acid bacteria (LAB) Kimchi. Based on 16S rRNA gene sequencing, LAB C2 was identified as a member genus Leuconostoc. obtained supernatant Leuconostoc sp. strain employed for green synthesis metal (AuNPs) oxide (ZnONPs) nanoparticles. UV-vis absorption spectra, FTIR analysis, XRD, DLS, FE-TEM, EDS mapping were used fully characterize these C2-AuNPs C2-ZnONPs. found spherical shape, size 47.77 ± 5.7 nm zeta potential -19.35 0.67 mV. C2-ZnONPs observed rod-shaped 173.77 14.53 size. determined 26.62 0.35 shown have activity against different pathogens. Furthermore, inhibited growth Candida albicans. antibiofilm antivirulence properties NPs Pseudomonas aeruginosa Staphylococcus aureus thoroughly investigated. reported P. aeruginosa, whereas both S. aureus. disrupted preformed mature biofilm inhibitory impact discovered concentration-dependent. research demonstrated that exhibited effects virulence features Further studies are needed unravel molecular mechanism behind inhibition attenuation.
Language: Английский
Citations
28
ACS Omega, Journal Year: 2023, Volume and Issue: 8(11), P. 9873 - 9888
Published: March 7, 2023
The emergence of antibiotic resistance in microbial pathogens necessitates the development alternative ways to combat infections that arise. current study used nanotechnology as an alternate technique control virulence characteristics and biofilm Pseudomonas aeruginosa Staphylococcus aureus. Furthermore, based on acceptance biocompatibility probiotic bacteria, we chose a lactic acid bacteria (LAB) for synthesizing two types metallic nanoparticles (NPs) this study. Using molecular techniques, LAB strain C1 was isolated from Kimchi food samples identified Lactiplantibacillus sp. C1. prepared supernatant produce gold (AuNPs) silver (AgNPs). C1-AuNPs C1-AgNPs were characterized physiochemically using variety instruments. had spherical shapes sizes 100.54 ± 14.07 nm 129.51 12.31 (AgNPs), respectively. discovered have high zeta potentials -23.29 1.17 -30.57 0.29 mV, These antibacterial properties against several bacterial pathogens. significantly inhibited initial stage formation effectively eradicated established mature biofilms P. S. when treated with sub-MIC levels C1-AgNPs, their different features reduced. Both NPs greatly hemolytic activity inhibition aureus by can be regarded viable therapeutic strategies preventing caused these bacteria.
Language: Английский
Citations
20
Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)
Published: Jan. 18, 2024
Language: Английский
Citations
8
Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 191, P. 106658 - 106658
Published: April 20, 2024
Language: Английский
Citations
6
Marine Drugs, Journal Year: 2021, Volume and Issue: 19(11), P. 601 - 601
Published: Oct. 22, 2021
With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, materials used for synthesis from natural sources are extracts. Natural extracts contain a wide range bioactive components, making it difficult to pinpoint exact component responsible nanoparticle synthesis. Furthermore, present in extract changes according numerous environmental factors. As result, current work intended synthesize gold (AuNPs) and zinc oxide (ZnONPs) using pure phloroglucinol (PG). The PG-AuNPs PG-ZnONPs were characterized UV–Vis absorption spectrophotometer, FTIR, DLS, FE-TEM, zeta potential, EDS, energy-dispersive X-ray diffraction. employed combat pathogenesis Pseudomonas aeruginosa. P. aeruginosa is recognized one prevalent pathogens common cause nosocomial infection humans. Antimicrobial resistance has linked development recalcitrant phenotypic characteristics, such biofilm, which identified major obstacles therapy. generates various virulence factors that chronic infection. These significantly inhibit early stage biofilm eradicate mature biofilm. these NPs reduce pyoverdine, pyocyanin, protease, rhamnolipid, hemolytic capabilities. addition, swarming, swimming, twitching motility. can be control infections caused biofilm-forming human pathogenic bacterium
Language: Английский
Citations
36
World Journal of Microbiology and Biotechnology, Journal Year: 2022, Volume and Issue: 38(10)
Published: July 29, 2022
Language: Английский
Citations
28