Deleted Journal,
Journal Year:
2023,
Volume and Issue:
1(1), P. 10002 - 10002
Published: Jan. 1, 2023
Unique
structural
features
and
wide
applications
of
gold
nanoparticles
(GNPs)
are
inspiring
researchers
to
develop
biocompatible,
reliable
cost-effective
methods
for
their
synthesis.
Herein,
a
clean,
eco-friendly
non-toxic
method
obtain
GNPs
was
developed
by
reducing
capping
the
liquid
extract
stem
Lilium
longiflorum
highlights
catalytic
reduction
4-nitrophenol
(4-NP)
methylene
blue
(MB).
The
formation
confirmed
through
absorption
peak
at
535
nm
in
UV-Vis
spectra.
TEM
HRTEM
analyses
reveal
spherical
morphology
with
an
average
size
4.97
nm.
SEM
EDX
further
elucidate
nature
elemental
composition.
FTIR
spectroscopy
analysis
demonstrates
that
were
coated
organic
compounds,
which
prevent
nanoparticle
from
aggregation.
exhibit
remarkable
efficiency
4-NP
MB.
efficacy
synthesized
demonstrated
enhanced
rates
MB,
rate
constants
1.50
min−1
1.29
min−1,
respectively.
This
study
develops
novel
technique
synthesis
opens
possibilities
green
other
metal
nanoparticles.
activity
holds
promise
range
industrial
environmental
sustainability.
Biofilm,
Journal Year:
2024,
Volume and Issue:
7, P. 100192 - 100192
Published: March 15, 2024
The
increasing
incidence
of
antimicrobial
resistance
exhibited
by
biofilm-forming
microbial
pathogens
has
been
recognized
as
one
the
major
issues
in
healthcare
sector.
In
present
study,
nanomaterial-based
controlling
biofilm
and
virulence
properties
considered
an
alternative
approach.
Pyoverdine
(PVD)
isolated
from
Pseudomonas
aeruginosa
was
utilized
a
biological
corona
to
synthesize
silver
nanoparticles
(AgNPs),
which
will
be
helpful
targeted
action
due
recognition
pathogenic
membrane.
Synthesized
PVD-AgNPs
were
spherical
irregular,
with
average
size
value
251.87
±
21.8
nm
zeta
potential
−36.51
0.69
mV.
MIC
towards
P.
aeruginosa,
Listeria
monocytogenes,
Staphylococcus
aureus,
Streptococcus
mutans,
Escherichia
coli,
Candida
albicans
standard
host-mimicking
media
observed
decreasing
order
multi-fold,
such
growth
>
sputum
synthetic
human
urine
saliva.
Both
initial
stage
well-established
biofilms
these
have
effectively
inhibited
eradicated
PVD-AgNPs.
increase
susceptibility
tetracycline,
PVD,
amphotericin
B
established
mature
mono-
mixed-species
S.
aureus
C.
albicans.
Additionally,
attenuate
several
properties,
inhibition
protease
activity,
motility,
PVD
pyocyanin
production
aeruginosa.
gene
expression
virulence-associated
genes
validates
its
phenotypic
effects.
Antibiotics,
Journal Year:
2024,
Volume and Issue:
13(7), P. 623 - 623
Published: July 4, 2024
Hospital-acquired
infections,
also
known
as
nosocomial
include
bloodstream
surgical
site
skin
and
soft
tissue
respiratory
tract
urinary
infections.
According
to
reports,
Gram-positive
Gram-negative
pathogenic
bacteria
account
for
up
70%
of
infections
in
intensive
care
unit
(ICU)
patients.
Biofilm
production
is
a
main
virulence
mechanism
distinguishing
feature
bacterial
pathogens.
Most
pathogens
develop
biofilms
at
the
solid-liquid
air-liquid
interfaces.
An
essential
requirement
biofilm
presence
conditioning
film.
A
film
provides
first
surface
on
which
can
adhere
fosters
growth
by
creating
favorable
environment.
The
improves
microbial
adherence
delivering
chemical
signals
or
generating
microenvironments.
Microorganisms
use
this
coating
nutrient
source.
gathers
both
inorganic
organic
substances
from
its
surroundings,
these
are
generated
microbes
These
nutrients
boost
initial
adhering
facilitate
formation
acting
food
Coatings
with
combined
antibacterial
efficacy
antifouling
properties
provide
further
benefits
preventing
dead
cells
debris
surfaces.
In
present
review,
we
address
numerous
that
form
surfaces
biomedical
devices.
addition,
explore
several
efficient
smart
antiadhesive
coatings
device-relevant
materials
manage
caused
biofilm-forming
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: Feb. 13, 2024
Abstract
The
rapidly
rising
antimicrobial
resistance
(AMR)
in
pathogenic
bacteria
has
become
one
of
the
most
serious
public
health
challenges,
with
a
high
death
rate.
Most
have
been
recognized
as
source
AMR
and
primary
barrier
to
treatment
failure
due
development
biofilms
production
virulence
factors.
In
this
work,
nanotechnology
was
employed
substitute
method
control
formation
attenuate
features
Pseudomonas
aeruginosa
Staphylococcus
aureus
.
We
synthesized
biocompatible
gold
nanoparticles
from
marine-derived
laminarin
potential
biofilm
treatments.
Laminarin-gold
(Lam-AuNPs)
identified
spherical,
49.84
±
7.32
nm
size
−
26.49
1.29
mV
zeta
potential.
MIC
value
Lam-AuNPs
against
several
drug-resistant
microbial
pathogens
varied
2
1024
μg/mL
both
standard
host-mimicking
media.
Sub-MIC
values
were
reported
effectively
reduce
P.
S.
growth
Furthermore,
sub-MIC
strongly
reduced
hemolysis,
pyocyanin,
pyoverdine,
protease,
forms
flagellar
pili-mediated
motility
also
inhibited
hemolysis
amyloid
fibrils.
dispersed
preformed
mature
these
dose-dependent
manner.
would
be
considered
an
alternative
antibiofilm
antivirulence
agent
infections.
Key
points
•
biosynthesized
virulence.
show
effective
inhibition
suppress
various
factors
aureus.
Journal of Global Antimicrobial Resistance,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
This
study
aims
to
investigate
the
effectiveness
of
UIO-66-NH2,
a
metal-organic
framework
(MOF),
as
carrier
for
imipenem/cilastatin
(Imp/Cil)
in
overcoming
resistance
clinical
isolates
imipenem-resistant
Pseudomonas
aeruginosa.
The
UIO-66-NH2-Imp/Cil
formulations
were
synthesized
and
characterized
using
dynamic
light
scattering
(DLS),
scanning
electron
microscopy
(SEM)
transmission
(TEM).
Drug
entrapment
efficiency
UIO-66-NH2-Imp/Cil,
Imp/Cil
release
rates
determined.
stability
was
assessed
by
at
room
temperature
refrigeration
two
months.
antibacterial,
anti-biofilm
anti-virulence
activities
investigated
against
aeruginosa
isolates.
formulation
showed
an
average
particle
size
212.3
±
7.3
nm,
polydispersity
index
(PDI)
0.142
0.010,
(EE%)
74.19
1.12%.
from
followed
Korsmeyer-Peppas
kinetic
model,
with
52%
drug
released
over
72
hours.
Antibacterial
testing
indicated
significant
decrease
minimum
inhibitory
concentration
(MIC)
bactericidal
(MBC)
compared
free
Imp/Cil,
demonstrating
enhanced
antibacterial
activity.
Furthermore,
activity
confirmed
reduction
bacterial
hemolysis
activity,
minimal
pyocyanin,
EPS
(extracellular
polymeric
substance)
production,
lower
metabolic
pathogens.
Also,
causes
expression
lasA,
lasB
and,
rhlA
genes,
which
resulted
inhibition
quorum-sensing
(QS)
system
These
findings
indicate
that
nanocarriers
offer
promising
new
approach
multidrug-resistant
Gram-negative
pathogens,
providing
insights
into
potential
mechanisms
antimicrobial
action.
