Carbon Catabolite Repression in Yeast is Not Limited to Glucose

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: April 24, 2019

Cells adapt their gene expression and metabolism in response to a changing environment. Glucose represses of genes involved the catabolism other carbon sources process known as (carbon) catabolite repression. However, relationships between "poor" is less characterized. Here we show that addition well-characterized glucose (and galactose) repression ADH2 (alcohol dehydrogenase 2, required for efficient utilization ethanol source), also inhibited by acetate which produced during catabolism. Thus, repressive regulation occurs sources. Acetate via Haa1, independently from mechanism AMPK (Snf1) activation Adr1. The extracellular attenuated when all three transporters (Ady2, Fps1 Jen1) are deleted, but these deletions do not affect resulting growth with or source. Furthermore, genetic manipulation catabolic pathway affects this response. Together, our results sensed intracellularly hierarchical control exists even

Language: Английский

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ATP hydrolysis by yeast Hsp104 determines protein aggregate dissolution and size in vivo

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Oct. 16, 2020

Abstract Signs of proteostasis failure often entwine with those metabolic stress at the cellular level. Here, we study protein sequestration during glucose deprivation-induced ATP decline in Saccharomyces cerevisiae . Using live-cell imaging, find that misfolded proteins and nascent polypeptides into two distinct compartments, granules, Q-bodies, is triggered by exhaustion ATP. Both compartments readily dissolve a PKA-dependent manner within minutes reintroduction level restoration. We identify hydrolase activity Hsp104 disaggregase as critical ATP-consuming process determining abundance size, even optimal conditions. Sequestration acute their retrieval recovery phase provide competitive fitness advantage, likely promoting cell survival stress.

Language: Английский

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pH homeostasis links the nutrient sensing PKA/TORC1/Sch9 ménage-à-trois to stress tolerance and longevity

Microbial Cell, Journal Year: 2018, Volume and Issue: 5(3), P. 119 - 136

Published: Feb. 21, 2018

pH homeostasis links the nutrient sensing PKA/TORC1/Sch9 ménage-à-trois to stress tolerance and longevity – INTRODUCTION is of crucial importance for many molecular physiological processes. In eukaryotic cells, intracellular affects protein folding enzyme activity, required vesicle trafficking impacts organelle function integrity. As our knowledge progresses, it becomes increasingly evident that proton not only acts as a facilitator (...)

Language: Английский

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γ-Glutamylcysteine amelioratesd-gal-induced senescence in PC12 cells and miceviaactivating AMPK and SIRT1

Food & Function, Journal Year: 2022, Volume and Issue: 13(14), P. 7560 - 7571

Published: Jan. 1, 2022

Aging is a natural process accompanied by inflammation and oxidative stress closely associated with age-related diseases. As direct precursor of glutathione, γ-glutamylcysteine (γ-GC) possesses antioxidant anti-inflammatory properties; however, whether γ-GC plays an important role in anti-aging remains unknown. Here, we investigated the protective effects mechanisms D-galactose (D-gal)-induced senescence PC12 cells aging mice. Our results showed that treatment significantly reduced percentage senescence-associated-β-galactosidase (SA-β-Gal)-positive inhibited D-gal-induced cell cycle arrest cells. The Nissl hematoxylin eosin (H&E) staining mouse brain markedly reversed damage hippocampus Moreover, increased phosphorylation AMP-activated protein kinase (AMPK) to promote nuclear translocation glyceraldehyde-3-phosphate dehydrogenase (GAPDH) while inhibiting deleted breast cancer 1 (DBC1), which leads activation sirtuin (SIRT1) deacetylation p53 nucleus. Therefore, may be potential therapeutic candidate compound for prevention

Language: Английский

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Frequent ploidy changes in growing yeast cultures

Current Genetics, Journal Year: 2018, Volume and Issue: 64(5), P. 1001 - 1004

Published: March 10, 2018

Language: Английский

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Mechanisms for the epigenetic inheritance of stress response in single cells

Current Genetics, Journal Year: 2018, Volume and Issue: 64(6), P. 1221 - 1228

Published: May 30, 2018

Language: Английский

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Interplay of mesoscale physics and agent-like behaviors in the parallel evolution of aggregative multicellularity

EvoDevo, Journal Year: 2020, Volume and Issue: 11(1)

Published: Oct. 12, 2020

Abstract Myxobacteria and dictyostelids are prokaryotic eukaryotic multicellular lineages, respectively, that after nutrient depletion aggregate develop into structures called fruiting bodies. The developmental processes resulting morphological outcomes resemble one another to a remarkable extent despite their independent origins, the evolutionary distance between them lack of traceable homology in molecular mechanisms. We hypothesize parallelism two lineages arises as consequence interplay within aggregates generic , physical physicochemical operating similarly living non-living matter at mesoscale (~10 –3 –10 –1 m) agent-like behaviors unique systems characteristic constituent cells, considered autonomous entities acting according internal rules shared environment. Here, we analyze contributions determinants myxobacteria dictyostelid development roles generation common traits. Consequent aggregation, collective cell–cell contacts mediate emergence liquid-like properties, making nascent masses subject novel patterning morphogenetic processes. In both this leads such streaming, rippling, rounding-up, seen fluids. Later solidify, leading exhibit additional properties motifs. Computational models suggest phenotypes deviate from predictions physics due contribution cells directed migration, quiescence, oscillatory signal transduction mediated by responses external cues. These employ signaling mechanisms reflect histories respective organisms. propose similar trajectories more coordination with analogous agent-type than convergent evolution under parallel selection regimes. Insights biology these aggregative forms may enable unified understanding evolution, including animals plants.

Language: Английский

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Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

Microbial Cell, Journal Year: 2021, Volume and Issue: 8(7), P. 146 - 160

Published: June 25, 2021

Ageing-related processes are largely conserved, with simple organisms remaining the main platform to discover and dissect new ageing-associated genes. Yeasts provide potent model systems study cellular ageing owing their amenability systematic functional assays under controlled conditions. Even yeast cells, however, can be laborious resource-intensive. Here we present improved experimental computational methods chronological lifespan in Schizosaccharomyces pombe. We decoded barcodes for 3206 mutants of latest gene-deletion library, enabling parallel profiling ~700 additional compared previous screens. then applied a refined method barcode sequencing (Bar-seq), addressing technical statistical issues raised by persisting DNA dead cells sampling bottlenecks aged cultures, screen showing altered during stationary phase. This identified 341 long-lived 1246 short-lived which point many previously unknown genes, including 46 conserved but entirely uncharacterized The genes showed coherent enrichments also associated human ageing, particularly respect non-proliferative brain cells. developed an automated colony-forming unit assay facilitate medium- high-throughput chronological-lifespan studies saving time resources traditional assay. Results from Bar-seq good agreement this provides effective methodological identifies as framework analysing beyond.

Language: Английский

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Glucosinolates from Seed-Press Cake of Camelina sativa (L.) Crantz Extend Yeast Chronological Lifespan by Modulating Carbon Metabolism and Respiration

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 80 - 80

Published: Jan. 11, 2025

Glucosinolates (GSLs) are nitrogen/sulfur-containing glycosides widely present in the order of Brassicales, particularly Brassicaceae family. Camelina (Camelina sativa (L.) Crantz) is an oilseed plant belonging to this Its seeds, addition a distinctive fatty acid composition, contain three aliphatic GSLs: glucoarabin, glucocamelinin, and homoglucocamelinin. Our study explored impact these GSLs purified from press cake, by-product oil production, on yeast chronological aging, which established model for simulating aging post-mitotic quiescent mammalian cells. Supplementing cells with extends lifespan (CLS) dose-dependent manner. This enhancement relies improved mitochondrial respiration efficiency, resulting drastic decrease superoxide anion levels increase ATP production. Furthermore, GSL supplementation affects carbon metabolism. In particular, support pro-longevity preservation TCA cycle enzymatic activities enhanced glycerol catabolism. These changes contribute positively phosphorylating trehalose storage: both longevity-promoting prerequisites.

Language: Английский

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The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(1), P. e3002997 - e3002997

Published: Jan. 28, 2025

The DAF-2/insulin/insulin-like growth factor signaling (IIS) pathway plays an evolutionarily conserved role in regulating reproductive development, life span, and stress resistance. In Caenorhabditis elegans , DAF-2/IIS is modulated by extensive array of insulin-like peptides (ILPs) with diverse spatial temporal expression patterns. However, the release dynamics specific functions these ILPs adapting to different environmental conditions remain poorly understood. Here, we show that ILP, insulin-3 (INS-3), a crucial modulating response various stressors C. . ins-3 mutants display increased resistance heat, oxidative stress, starvation; however, this advantage countered slower development under favorable conditions. We find downregulated stressors, whereas, neurohormone tyramine, which released during acute flight response, increases expression. tyramine induces intestinal calcium (Ca 2+ ) transients through activation TYRA-3 receptor. Our data support model negatively impacts stimulating INS-3 from intestine via TYRA-3-G α q -IP3 pathway. systemically activates DAF-2 pathway, resulting inhibition cytoprotective mechanisms mediated DAF-16/FOXO. These studies offer mechanistic insights into brain–gut communication weighs adaptive strategies respond long-term stressors.

Language: Английский

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