Multipotent Embryonic Lung Progenitors: Foundational Units of In Vitro and In Vivo Lung Organogenesis DOI Creative Commons
Laertis Ikonomou, Maria Yampolskaya, Pankaj Mehta

et al.

Advances in experimental medicine and biology, Journal Year: 2023, Volume and Issue: unknown, P. 49 - 70

Published: Jan. 1, 2023

Abstract Transient, tissue-specific, embryonic progenitors are important cell populations in vertebrate development. In the course of respiratory system development, multipotent mesenchymal and epithelial drive diversification fates that results to plethora types compose airways alveolar space adult lungs. Use mouse genetic models, including lineage tracing loss-of-function studies, has elucidated signaling pathways guide proliferation differentiation lung as well transcription factors underlie progenitor identity. Furthermore, pluripotent stem cell-derived ex vivo expanded offer novel, tractable, high-fidelity systems allow for mechanistic studies fate decisions developmental processes. As our understanding biology deepens, we move closer goal vitro organogenesis resulting applications medicine.

Language: Английский

Extracellular matrix dynamics: tracking in biological systems and their implications DOI Creative Commons
Michael Hu, Zihan Ling, Xi Ren

et al.

Journal of Biological Engineering, Journal Year: 2022, Volume and Issue: 16(1)

Published: May 30, 2022

Abstract The extracellular matrix (ECM) constitutes the main acellular microenvironment of cells in almost all tissues and organs. ECM not only provides mechanical support, but also mediates numerous biochemical interactions to guide cell survival, proliferation, differentiation, migration. Thus, better understanding everchanging temporal spatial shifts composition structure – dynamics will provide fundamental insight regarding regulation tissue homeostasis how states transition from one another during diverse pathophysiological processes. This review outlines mechanisms mediating ECM-cell highlights changes modulate development disease progression, using lung as primary model organ. We then discuss existing methodologies for revealing compositional dynamics, with a particular focus on tracking newly synthesized proteins. Finally, we ramifications have engineering implement specific microenvironments into bioengineered tissues. Overall, this communicates current capabilities studying native delineates new research directions discovering implementing push frontier forward.

Language: Английский

Citations

87
Lung Organoids—The Ultimate Tool to Dissect Pulmonary Diseases? DOI Creative Commons
Veronika Bosáková, Marco De Zuani,

Lucie Sládková

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: July 13, 2022

Organoids are complex multicellular three-dimensional (3D) in vitro models that designed to allow accurate studies of the molecular processes and pathologies human organs. can be derived from a variety cell types, such as primary progenitor cells, pluripotent stem or tumor-derived cells co-cultured with immune microbial further mimic tissue niche. Here, we focus on development 3D lung organoids their use disease drug screening tools. We introduce various experimental approaches used model diseases analyze advantages disadvantages. also discuss validation physiological relevance study diseases. Furthermore, summarize current host-pathogen interactions cystic fibrosis, chronic obstructive pulmonary disease, SARS-CoV-2 infection. Moreover, tumor cancer application personalized medicine research. Finally, outline future research field induced cell-derived organoids.

Language: Английский

Citations

30
The Regenerative Power of Stem Cells: Treating Bleomycin-Induced Lung Fibrosis DOI Creative Commons
Amrita Vats, Pankaj Chaturvedi

Stem Cells and Cloning Advances and Applications, Journal Year: 2023, Volume and Issue: Volume 16, P. 43 - 59

Published: Sept. 1, 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with no known cure, characterized by the formation of scar tissue in lungs, leading to respiratory failure.Although exact cause IPF remains unclear, condition thought result from combination genetic environmental factors.One most widely used animal models study bleomycin-induced injury model mice.In this model, administration chemotherapeutic agent bleomycin causes inflammation fibrosis, which closely mimics pathological features human IPF.Numerous recent investigations have explored functions various categories stem cells healing process induced mice, documenting beneficial effects challenges approach.Differentiation into cell types their ability modulate microenvironment an emerging aspect regenerative therapies.This review article aims provide comprehensive overview role repairing injury.It delves mechanisms through cells, including mesenchymal embryonic pluripotent resident exert therapeutic specific model.We also discussed unique set intermediate markers signaling factors that can influence proliferation differentiation alveolar epithelial both during repair homeostasis.Finally, we highlight opportunities associated translating therapy clinic for patients.The novelty implications extend beyond understanding potential treating broader field medicine.We believe paves way further advancements therapies, offering hope patients suffering debilitating currently incurable disease.

Language: Английский

Citations

13
A Niche-Based Perspective to Stem and Cancer Stem Cells of the Lung DOI
Özge Boyacıoğlu,

Behnam Kalali,

Ege Tongün

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
How to use an in vitro approach to characterize the toxicity of airborne compounds DOI Creative Commons

Nour Jaber,

Sylvain Billet

Toxicology in Vitro, Journal Year: 2023, Volume and Issue: 94, P. 105718 - 105718

Published: Oct. 21, 2023

Language: Английский

Citations

7
The interplay between hyaluronic acid and stem cell secretome boosts pulmonary differentiation in 3D biomimetic microenvironments DOI Creative Commons
Francesca Della Sala, Gennaro Longobardo,

Mario di Gennaro

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133793 - 133793

Published: July 9, 2024

Mesenchymal stem cells (MCSs) secretome provide MSC-like therapeutic effects in preclinical models of lung injury, circumventing safety concerns with the use live cells. Secretome consists Extracellular Vesicles (EVs), including populations nano- to micro-sized particles (exosomes and microvesicles) delimited by a phospholipidic bilayer. However, its poor stability bioavailability severely limit application. The role Hyaluronic acid (HA) as potential carrier biomedical applications has been widely demonstrated. Here, we investigated interplay between HA MSCs- blends their ability exert bioactive effect on pulmonary differentiation 3D microenvironment mimicking niche. To this aim, physical-chemical properties HA/Secre have characterized at low, medium high Molecular Weights (MWs), means SEM/TEM, DLS, confocal microscopy FTIR. Collectively highlight EVs. In matrices, showed promote lineage, improved MW decreased. Finally, blends' cross an artificial mucus Overall, work provides new insights for development future devices therapy respiratory diseases that are still unmet.

Language: Английский

Citations

2
Lung Progenitor and Stem Cell Transplantation as a Potential Regenerative Therapy for Lung Diseases DOI

V. Villa,

Xi Ren

Transplantation, Journal Year: 2024, Volume and Issue: 108(10), P. e282 - e291

Published: Feb. 28, 2024

Chronic lung diseases are debilitating illnesses ranking among the top causes of death globally. Currently, clinically available therapeutic options capable curing chronic limited to transplantation, which is hindered by donor organ shortage. This highlights urgent need for alternative strategies repair damaged tissues. Stem cell transplantation has emerged as a promising avenue regenerative treatment lung, involves delivery healthy epithelial progenitor cells that subsequently engraft in injured tissue and further differentiate reconstitute functional respiratory epithelium. These transplanted possess remarkable ability self-renew, thereby offering potential sustained long-term effects. Notably, basal cells, airway stem holds promise rehabilitating injuries resulting from environmental factors or genetic conditions such cystic fibrosis. Similarly, affecting alveoli, alveolar type II have garnered interest viable source restoring parenchyma environmentally induced dysfunctions. Expanding upon these advancements, use pluripotent derive offers advantages scalability patient specificity. In this review, we comprehensively explore progress made providing insights into current state field its future prospects.

Language: Английский

Citations

2
Engineering Cell Instructive Microenvironments for In Vitro Replication of Functional Barrier Organs DOI
Francesco Urciuolo, Giorgia Imparato,

Paolo Antonio Netti

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: May 2, 2024

Multicellular organisms exhibit synergistic effects among their components, giving rise to emergent properties crucial for genesis and overall functionality survival. Morphogenesis involves relies upon intricate biunivocal interactions cells environment, that is, the extracellular matrix (ECM). Cells secrete own ECM, which in turn, regulates morphogenetic program by controlling time space presentation of matricellular signals. The once considered passive, is now recognized as an informative where both biochemical biophysical signals are tightly orchestrated. Replicating this sophisticated highly interconnected media a synthetic scaffold tissue engineering unattainable with current technology limits capability engineer functional human organs vitro vivo. This review explores limitations organ morphogenesis, emphasizing interplay gene regulatory networks, mechanical factors, microenvironment cues. In efforts replicate biological processes barrier such lung intestine, examined. importance maintaining within native microenvironmental context highlighted accurately organ-specific properties. underscores necessity microphysiological systems faithfully reproduce cell-native interactions, advancing understanding developmental disorders disease progression.

Language: Английский

Citations

2
Stem cells, Notch-1 signaling, and oxidative stress: a hellish trio in cancer development and progression within the airways. Is there a role for natural compounds? DOI
Giuseppina Chiappara, Serena Di Vincenzo,

Caterina Cascio

et al.

Carcinogenesis, Journal Year: 2024, Volume and Issue: 45(9), P. 621 - 629

Published: July 24, 2024

Abstract Notch-1 signaling plays a crucial role in stem cell maintenance and repair mechanisms various mucosal surfaces, including airway mucosa. Persistent injury can induce an aberrant activation of cells leading to increased risk cancer initiation progression. Chronic inflammatory respiratory disorders, chronic obstructive pulmonary disease (COPD) is associated with both overactivation lung risk. Increased oxidative stress, also due cigarette smoke, further contribute promote progression by amplifying responses, activating the signaling, blocking regulatory that inhibit growth capacity cells. This review offers comprehensive overview effects stress cancer. The putative natural compounds antioxidant properties described.

Language: Английский

Citations

1
Transcriptomic Analysis of Air–Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation DOI Creative Commons

J. Kim,

Eun-Young Eo,

Bokyong Kim

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(23), P. 1991 - 1991

Published: Dec. 2, 2024

To develop in vitro respiratory models, it is crucial to identify the factors involved epithelial cell differentiation. In this study, we comprehensively analyzed effects of air-liquid interface (ALI) culture on differentiation using single-cell RNA sequencing (scRNA-seq). ALI induced a pronounced shift composition, marked by fivefold increase ciliated cells and reduction more than half basal cells. Transcriptional signatures associated with differentiation, iPathwayGuide software, revealed downregulation

Language: Английский

Citations

1