Recent technological advances in lab-on-a-chip for bone remodeling DOI Creative Commons
Seoyeon Kim, Arun Rajendran, Sivashanmugam Amirthalingam

et al.

Biosensors and Bioelectronics X, Journal Year: 2023, Volume and Issue: 14, P. 100360 - 100360

Published: June 6, 2023

Lab-on-a-chip (LOC) is a three-dimensional cell culture platform with microfluidic flow that biomimics the physiological system of various organs. LOC emerged as future replacement technology for in vitro cell-based models and vivo animal models, gaining popularity field physiology, pharmacology, biology. Nowadays, sensors are incorporated into to monitor real-time parameters its microenvironment response biophysical and/or biochemical stimuli maturation. A considerable amount studies has been conducted detect mechanical, physical, electrical, metabolic engineered This paper aims provide comprehensive insight how biosensor-implemented can be utilized analysis tissue recapitulating systems focus on bone remodeling. We describe chip design, basic principles sensors, ongoing recent efforts perform in-line organoids. review also presents imaging techniques used analytical tools visualizing LOC.

Language: Английский

Inhibitors of the Elastase LasB for the Treatment of Pseudomonas aeruginosa Lung Infections DOI Creative Commons
Jelena Konstantinović, Andreas M. Kany, Alaa Alhayek

et al.

ACS Central Science, Journal Year: 2023, Volume and Issue: 9(12), P. 2205 - 2215

Published: Oct. 27, 2023

Infections caused by the Gram-negative pathogen Pseudomonas aeruginosa are emerging worldwide as a major threat to human health. Conventional antibiotic monotherapy suffers from rapid resistance development, underlining urgent need for novel treatment concepts. Here, we report on nontraditional approach combat P. aeruginosa-derived infections targeting its main virulence factor, elastase LasB. We discovered new chemical class of phosphonates with an outstanding in vitro ADMET and PK profile, auspicious activity both vivo. established mode action through cocrystal structure our lead compound LasB several ex vivo models. The proof concept combination pathoblocker levofloxacin murine neutropenic lung infection model reduction protein levels blood target engagement demonstrate great potential use adjunctive humans.

Language: Английский

Citations

9
Lung Organoids in Smoking Research: Current Advances and Future Promises DOI Creative Commons
Hina Agraval, Hong Wei Chu

Biomolecules, Journal Year: 2022, Volume and Issue: 12(10), P. 1463 - 1463

Published: Oct. 12, 2022

Tobacco smoking has been established to contribute the pathogenesis of various respiratory diseases including chronic obstructive pulmonary disease (COPD), lung cancer, and asthma. However, major hurdles in mechanistic studies on role human lungs remain part due lack ex vivo experimental models ambiguous data from animal that can best recapitulate architecture pathophysiology lung. Recent development organoid culture system opened new avenues for research as organoids are proving be a sophisticated model functionally structurally mimics better than other traditionally used models. This review will discuss how recent advances systems may help us determine injurious immunological effect provide some suggestions future directions.

Language: Английский

Citations

14
Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease DOI

Neeti N. Gandhi,

Thomas J. Inzana, Padmavathy Rajagopalan

et al.

ACS Infectious Diseases, Journal Year: 2023, Volume and Issue: 9(6), P. 1168 - 1179

Published: May 31, 2023

Bovine respiratory disease (BRD) is a multifactorial condition where different genera of bacteria, such as Mannheimia haemolytica, Histophilus somni, Pasteurella multocida, and Mycoplasma bovis, viruses, like bovine syncytial virus, viral diarrhea herpes virus-1, infect the lower tract cattle. These pathogens can co-infect cells in system, thereby making specific treatment very difficult. Currently, most common models for studying BRD include submerged tissue culture (STC), monolayers epithelial are typically covered either cellular or spent biofilm medium. Another model an air–liquid interface (ALI), exposed on their apical side allowed to differentiate. However, limited work has been reported study three-dimensional (3D) that incorporate multiple cell types represent architecture tract. The roles defense mechanisms infected mucin production, tight junction barriers, production antimicrobial peptides vitro cultures require further investigation order provide comprehensive understanding pathogenesis. In this report, we describe aspects BRD, including implicated tract, which important assembled vitro. Although current advancements have led knowledge disease, 3D multicellular organoids better recapitulate vivo environment exhibit potential future investigations.

Language: Английский

Citations

8
In vitro data for fire pollutants: contribution of studies using human cell models towards firefighters’ occupational DOI
Maria João Bessa, Bruno Sarmento, Marta Oliveira

et al.

Journal of Toxicology and Environmental Health Part B, Journal Year: 2023, Volume and Issue: 26(4), P. 238 - 255

Published: March 8, 2023

Firefighters are the principal line of defense against fires, being at elevated risk exposure to health-relevant pollutants released during fires and burning processes. Although many biomonitoring studies exist, only a limited number human in vitro investigations fire assessment currently available. In stand out as valuable tools assess toxicity mechanisms involved following cellular level. The aim present review was contextualize existing using cell models exposed chemicals emitted from emissions wood smoke discuss implications observed toxic outcomes on adverse health effects detected firefighters. Most reported focused monocultures respiratory particulate matter (PM) extracts collected effluents. Overall, (1) decrease viability, (2) enhanced oxidative stress, (3) increased pro-inflammatory cytokines levels (4) death frequencies were noted. However, information remains regarding initiated by firefighting activities. Hence, more employing advanced systems lines urgently needed taking into consideration different routes health-related fires. Data establish define firefighters' occupational limits propose mitigation strategies promote beneficial health.

Language: Английский

Citations

7
Network pharmacology-based research into the mechanism of ferulic acid on acute lung injury through enhancing transepithelial sodium transport DOI
Yiman Zhai, Tong Yu, Shuning Xin

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118230 - 118230

Published: April 20, 2024

Language: Английский

Citations

2
Dynamic 3D in vitro lung models: applications of inorganic nanoparticles for model development and characterization DOI

Laura Fallert,

Ane Urigoitia-Asua,

Amaia Cipitria

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(23), P. 10880 - 10900

Published: Jan. 1, 2024

Being a vital organ exposed to the external environment, lung is susceptible plethora of pathogens and pollutants. This reflected in high incidences chronic respiratory diseases, which remain leading cause mortality world-wide pose persistent global burden. It thus paramount importance improve our understanding these pathologies provide better therapeutic options. necessitates development representative physiologically relevant vitro models. Advances bioengineering have enabled sophisticated models that not only capture three-dimensional architecture cellular environment but also incorporate dynamics local biophysical stimuli. However, such complex require novel approaches reliable characterization. Within this review we explore how 3D bioprinting nanoparticles can serve as multifaceted tools develop dynamic 4D printed facilitate their characterization context pulmonary fibrosis breast cancer metastasis.

Language: Английский

Citations

2
A drug-responsive multicellular human spheroid model to recapitulate drug-induced pulmonary fibrosis DOI
Ecem Saygılı, Utku Devamoglu, Bakiye Göker Bağca

et al.

Biomedical Materials, Journal Year: 2022, Volume and Issue: 17(4), P. 045021 - 045021

Published: May 26, 2022

Abstract Associated with a high mortality rate, pulmonary fibrosis (PF) is the end stage of several interstitial lung diseases. Although many factors are linked to PF progression, initiation fibrotic process remains be studied. Current research focused on generating new strategies gain better understanding underlying disease mechanism as animal models remain insufficient reflect human physiology. Herein, account complex cellular interactions within tissue, multicellular spheroid model where bronchial epithelial cells incorporated fibroblasts was generated and treated bleomycin (BLM) emulate drug-induced PF. Recapitulating epithelial-interstitial microenvironment, findings successfully reflected disease, excessive alpha smooth muscle actin collagen type I secretion were noted along morphological changes in response BLM. Moreover, increased levels COL13A1, MMP2, WNT3 decreased expression level CDH1 provide evidence for reliability modelling. Subsequent administration Food Drug Administration approved nintedanib pirfenidone anti-fibrotic drugs proved drug-responsiveness model.

Language: Английский

Citations

10
A prospects tool in virus research: Analyzing the applications of organoids in virus studies DOI
Qi Shen, Yuhan Zhou, Yanqiu Zhou

et al.

Acta Tropica, Journal Year: 2024, Volume and Issue: 254, P. 107182 - 107182

Published: March 11, 2024

Language: Английский

Citations

1
Review of in-vitro studies evaluating respiratory toxicity of aerosols: impact of cell types, chemical composition, and atmospheric processing DOI
Sudheer Salana, Vishal Verma

Environmental Science Processes & Impacts, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

In recent decades, several cell-based and acellular methods have been developed to evaluate ambient particulate matter (PM) toxicity. Although provide a more comprehensive assessment of PM toxicity, their results are difficult comprehend due the diversity in cellular endpoints, cell types, assays interference chemical components with some assays' techniques. this review, we attempt clarify these issues. We first discuss morphological immunological differences among various macrophage epithelial cells, belonging respiratory systems human murine species, used

Language: Английский

Citations

1
Two-Dimensional and Three-Dimensional Cell Culture and Their Applications DOI Creative Commons
Sangeeta Ballav, Ankita Jaywant Deshmukh, Shafina Siddiqui

et al.

Biochemistry, Journal Year: 2021, Volume and Issue: unknown

Published: Oct. 20, 2021

Cell culture is one of the most important and commonly used in vitro tools to comprehend various aspects cells or tissues a living body such as cell biology, tissue morphology, mechanism diseases, signaling, drug action, cancer research also finds its great importance preclinical trials drugs. There are two major types cultures that used- two-dimensional (2D) three-dimensional (3D). The former has been since 1900s, owing simplicity low-cost maintenance it forms monolayer, while latter being advanced version currently worked upon. This chapter intends provide true meaning significance both cultures. It starts by making clear distinction between proceeds further discuss their different applications vitro. 2D projected through assays therapeutic treatment understand motility whereas 3D includes models spheroid structures consisting multiple layers cells, puts light on use discovery development. concluded with detailed account production proteins cells.

Language: Английский

Citations

8