Development and characterization of an immortalized nasopharyngeal epithelial cell line to explore airway physiology and pathology in yak (Bos grunniens) DOI Creative Commons
Jiancheng Qi, Jizong Zhang,

Fangyuan Huang

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 17, 2024

Airway epithelial cells play a crucial role in investigating the physiological and pathological mechanisms of respiratory tract yaks, species whose unique system has garnered extensive interest. Despite this growing interest, there currently are no available airway cell lines from underscoring need to establish yak line. Therefore, our objective was isolate population primary nasopharyngeal (pYNE) transform them into immortalized (iYNE), assessing their suitability as an

Language: Английский

When the solution becomes the problem: a review on antimicrobial resistance in dairy cattle DOI
Ana José Pires, Gonçalo Pereira, David Fangueiro

et al.

Future Microbiology, Journal Year: 2024, Volume and Issue: 19(10), P. 903 - 929

Published: April 25, 2024

Antibiotics' action, once a 'magic bullet', is now hindered by widespread microbial resistance, creating global antimicrobial resistance (AMR) crisis. A primary driver of AMR the selective pressure from use. Between 2000 and 2015, antibiotic consumption increased 65%, reaching 34.8 billion tons, 73% which was used in animals. In dairy cattle sector, antibiotics are crucial for treating diseases like mastitis, posing risks to humans, animals potentially leading environmental contamination. To address AMR, strategies dry cow therapy, alternative treatments (nanoparticles, phages) waste management innovations emerging. However, most solutions development, emphasizing urgent need further research tackle farms.

Language: Английский

Citations

4

Role of biofilms in antimicrobial resistance of the bacterial bovine respiratory disease complex DOI Creative Commons
Sara Andrés-Lasheras, Rahat Zaheer,

Murray Jelinski

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: June 12, 2024

An increase in chronic, non-responsive bovine respiratory disease (BRD) infections North American feedlot cattle is observed each fall, a time when are administered multiple antimicrobial treatments for BRD. A number of factors responsible BRD treatment failure, with formation biofilms possibly being one. It widely accepted that play role chronic humans and it has been hypothesized they the default lifestyle most bacteria. However, research on bacterial associated livestock scarce significant knowledge gaps exist our understanding their AMR complex. The four main species complex, Mannheimia haemolytica , Pasteurella multocida Histophilus somni Mycoplasma bovis able to form vitro there evidence at least H. retains this ability vivo . need elucidate whether biofilm-forming contributes pathogenicity failure Overall, better possible clinical could assist prevention management cattle. We review discuss current bacteria biofilm biology, study methodologies, relationship AMR.

Language: Английский

Citations

4

Investigation of the safety and protective efficacy of an attenuated and marker M. bovis-BoHV-1 combined vaccine in bovines DOI Creative Commons
Sen Zhang, Guoxing Liu, Yisheng Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 4, 2024

Bovine respiratory disease (BRD) is one of the most common diseases in cattle industry worldwide; it caused by multiple bacterial or viral coinfections, which Mycoplasma bovis ( M. ) and bovine herpesvirus type 1 (BoHV-1) are notable pathogens. Although live vaccines have demonstrated better efficacy against BRD induced both pathogens, there no combined marker vaccines. Therefore, we developed an attenuated -BoHV-1 vaccine based on HB150 BoHV-1 gG-/tk- strain previously constructed our lab evaluated rabbits. This study aimed to further evaluate its safety protective using different antigen ratios. After immunization, all vaccinated had a normal rectal temperature mental status without symptoms. CD4 + , CD8 CD19 cells significantly increased immunized higher humoral cellular immune responses, expression key cytokines such as IL-4, IL-12, TNF-α, IFN-γ can be promoted after vaccination. The 1.0 × 10 8 CFU 6 TCID 50 elicited antibodies while increasing IgG immunity challenge. In conclusion, was clinically safe calves; mix promising due low amount shedding highest responses compared with others. introduces for application industry.

Language: Английский

Citations

2

Measurement of Human and Bovine Exhaled Breath Condensate pH Using Polyaniline-Modified Flexible Inkjet-Printed Nanocarbon Electrodes DOI Creative Commons
Aaron Jacobs, Maiyara Carolyne Prete, Andreas Lesch

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(39), P. 40841 - 40856

Published: Sept. 16, 2024

The collection, processing, and electrochemical analysis of exhaled breath condensate (EBC) from healthy human animal subjects is reported on. EBC a biospecimen potentially rich in biomarkers respiratory disease. pH was analyzed potentiometrically using disposable polyaniline (PANI)-modified inkjet-printed (IJP) carbon electrode. Comparison measurements were performed commercial screen-printed (SPC) PANI-modified electrodes exhibited reproducible near-Nernstian responses for values between 2 9 with slopes -50 to -60 mV/dec. IJP electrode faster response time superior reproducibility the modified SPC In proof-of-concept studies, found be 6.57 ± 0.09 bovine 5.9 0.2. All determined good agreement micro glass An RTube device used collect humans while calves field. volumes 0.5-2 mL 5-6 min tidal breathing collected animals. (17 animals) depends on their age 1 weeks ranging 5.3 7.2. A distinct alkaline shift observed many animals around 20 days age. also ambient temperature humidity at collection. results indicate that outperform provide accurate measurement across various types.

Language: Английский

Citations

1

Relationship between capsule production and biofilm formation by Mannheimia haemolytica, and establishment of a poly-species biofilm with other Pasteurellaceae DOI Creative Commons
Yue‐Jia Lee, Dianjun Cao, Bindu Subhadra

et al.

Biofilm, Journal Year: 2024, Volume and Issue: 8, P. 100223 - 100223

Published: Sept. 28, 2024

Language: Английский

Citations

1

The Safety and Protective Efficacy Evaluation of an Attenuated M. bovis–BoHV-1 Bivalent Vaccine in Rabbits DOI Creative Commons
Sen Zhang, Yisheng Zhang, Guoxing Liu

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(11), P. 1698 - 1698

Published: Nov. 7, 2023

Bovine respiratory disease (BRD) is a global prevalent multifactorial infection primarily caused by viral and bacterial coinfections. In China, Mycoplasma bovis (M. bovis) bovine herpesvirus type 1 (BoHV-1) are the predominant pathogens associated with BRD. Our previous study involved development of attenuated M. HB150 BoHV-1 gG-/tk- vaccine strains, which were thoroughly assessed for their safety profiles protective efficacy in cattle. this study, we applied combination vaccines varying ratios used rabbit model to determine efficacy. We PCR/RT-PCR detect postimmunization challenge shedding BoHV-1. Additionally, measured antibody titers expression IFN-β TNF-α evaluate humoral cellular immune responses, respectively. Furthermore, performed histopathological analysis assess lung damage. provides evidence effectiveness bivalent bovis-BoHV-1 rabbits, particularly when applying 1.0 × 108 CFU 106 TCID50 strain. The significantly enhanced both long-term response protection against challenge. These findings provide valuable potential application

Language: Английский

Citations

2

Investigation of the Optimal Immunization Dose and Protective Efficacy of an Attenuated and Marker M. bovis–Bovine Herpesvirus Type 1 Combined Vaccine in Rabbits DOI Creative Commons
Sen Zhang, Guoxing Liu,

Wenying Wu

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(5), P. 748 - 748

Published: Feb. 28, 2024

Bovine respiratory disease (BRD) is one of the most common diseases in cattle industry; it a globally prevalent multifactorial infection primarily caused by viral and bacterial coinfections. In China,

Language: Английский

Citations

0

Development and characterization of an immortalized nasopharyngeal epithelial cell line to explore airway physiology and pathology in yak (Bos grunniens) DOI Creative Commons
Jiancheng Qi, Jizong Zhang,

Fangyuan Huang

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 17, 2024

Airway epithelial cells play a crucial role in investigating the physiological and pathological mechanisms of respiratory tract yaks, species whose unique system has garnered extensive interest. Despite this growing interest, there currently are no available airway cell lines from underscoring need to establish yak line. Therefore, our objective was isolate population primary nasopharyngeal (pYNE) transform them into immortalized (iYNE), assessing their suitability as an

Language: Английский

Citations

0