Exploring the mechanisms of WRKY transcription factors and regulated pathways in response to abiotic stress

Plant Stress, Journal Year: 2024, Volume and Issue: 12, P. 100429 - 100429

Published: March 12, 2024

The environmental conditions encompassing plants exert a significant impact on their appropriate growth and development. It is of utmost importance to investigate the mechanisms signaling cascades underlying tolerance abiotic stress in order enhance quality crops. Plant development processes are significantly impacted by stresses, which intricately linked surroundings. Plants exhibit prompt genetic metabolic network responses, mostly through networks involving transcription factors that respond stress, including WRKY, MYB, bZIP, AP2/EREBP, NAC. Among these WRKY TFs factors, fulfill pivotal function diverse range responses developmental mechanisms. greatly assist coping with stress. These oversee control several target gene categories active involvement numerous interaction W-box cis-acting elements located promoters genes. This research provides comprehensive analysis response mechanism In addition, we have explored state knowledge TFs' effects plants' such as drought, salt, high temperatures, cold. elucidates intricate molecular govern pathways modulate expression, thereby conferring upon plants. Moreover, summarized involved biotic TFs, hormonal routes like SA JA, aid inducing resistance coordinating defense against pathogens challenges. agricultural sustainability augment crop resilience towards strategies manipulate regulatory need be established.

Language: Английский

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WRKY transcription factors: Hubs for regulating plant growth and stress responses

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

As sessile organisms, plants must directly face various stressors. Therefore, have evolved a powerful stress resistance system and can adjust their growth development strategies appropriately in different stressful environments to adapt complex ever-changing conditions. Nevertheless, prioritizing defensive responses hinder growth; this is crucial factor for plant survival but detrimental crop production. such, comprehending the impact of adverse on not only fundamental scientific inquiry also imperative agricultural industry food security. The traditional view that hindered during defense due resource allocation trade-offs challenged by evidence exhibit both robust capabilities through human intervention. These findings suggest growth‒defense trade-off dictated limitations influenced intricate transcriptional regulatory mechanisms. Hence, it conduct thorough investigations central genes govern unfavorable environments. Recent studies consistently highlighted importance WRKY transcription factors orchestrating plant-specific development, underscoring pivotal role WRKYs modulating under Here, we review recent advances understanding dual roles regulation across diverse This information will be elucidating interplay between response may aid identifying gene loci could utilized future breeding programs develop crops with enhanced productivity.

Language: Английский

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Large-scale analysis of the ARF and Aux/IAA gene families in 406 horticultural and other plants

Molecular Horticulture, Journal Year: 2024, Volume and Issue: 4(1)

Published: April 9, 2024

Abstract The auxin response factor (ARF) and auxin/indole-3-acetic acid (Aux/IAA) family of genes are central components the signaling pathway play essential roles in plant growth development. Their large-scale analysis evolutionary trajectory origin currently not known. Here, we identified corresponding ARF Aux/IAA members performed a by scanning 406 genomes. results showed that gene families originated from charophytes. sequences were more conserved than sequences. Dispersed duplications common expansion mode bryophytes, ferns, gymnosperms; however, whole-genome duplication was basal angiosperms, magnoliids, monocots, dicots. Expression regulatory network analyses revealed Arabidopsis thaliana responded to multiple hormone, biotic, abiotic stresses. APETALA2 serum factor-transcription commonly enriched upstream downstream families. Our study provides comprehensive overview trajectories, structural functions, mechanisms, expression patterns, networks these two

Language: Английский

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Pathogenesis related-1 proteins in plant defense: regulation and functional diversity

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: May 8, 2024

Climate change-related environmental stresses can negatively impact crop productivity and pose a threat to sustainable agriculture. Plants have remarkable innate ability detect broad array of cues, including that trigger stress-induced regulatory networks signaling pathways. Transcriptional activation plant pathogenesis related-1 (PR-1) proteins was first identified as an integral component systemic acquired resistance in response stress. Consistent with their central role immune defense, overexpression PR-1s diverse species is frequently used marker for salicylic acid (SA)-mediated defense responses. Recent advances demonstrated how virulence effectors, SA cascades, epigenetic modifications modulate PR-1 expression stresses. We others showed transcriptional involving could be improve resilience Together, the results these studies re-energized field provided long-awaited insights into possible function under extreme

Language: Английский

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Deciphering the Role of Epigenetics in Plant Pathogen Resistance: Strategies for Sustainable Sugarcane Management

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: Jan. 1, 2025

Language: Английский

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Multi-omics analyses reveal the responses of wheat (Triticum aestivum L.) and rhizosphere bacterial community to nano(micro)plastics stress

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 23, 2024

The pervasive existence of nanoplastics (NPs) and microplastics (MPs) in soil has become a worldwide environmental concern. N/MPs exist the environment variety forms, sizes, concentrations, while multi-omics studies on comprehensive impact with different properties (e.g. type size) plants remain limited. Therefore, this study utilized analysis methods to investigate effects three common polymers [polyethylene-NPs (PE-NPs, 50 nm), PE-MPs (PE-MPs, 10 μm), polystyrene-MPs (PS-MPs, μm)] growth stress response wheat, as well rhizosphere microbial community at two concentrations (0.05 0.5 g/kg). PS PE exhibited for same particle size concentration. PE-NPs had most severe effects, resulting reduced bacteria diversity, plant biomass, antioxidant enzyme activity increasing beneficial richness. altered expression nitrogen-, phosphorus-, sulfur-related functional genes bacteria, thereby affecting photosynthesis, metabolite gene levels wheat leaves. Partial least squares pathway models (PLSPMs) indicated that concentration, size, play important roles ecological environment, which could have essential implications assessing risk N/MPs.

Language: Английский

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Ecotype‐specific phenolic acid accumulation and root softness in Salvia miltiorrhiza are driven by environmental and genetic factors

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Summary Salvia miltiorrhiza Bunge, a renowned medicinal herb in traditional Chinese medicine, displays distinctive root texture and high phenolic acid content, traits influenced by genetic environmental factors. However, the underlying regulatory networks remain unclear. Here, we performed multi‐omics analyses on ecotypes from four major regions, focusing impacts structure, accumulation lignin composition. Lower temperatures increased UV‐B radiation were associated with elevated rosmarinic (RA) salvianolic B (SAB) levels, particularly Sichuan ecotype. Structural models indicated that radial arrangement of xylem conduits contributes to greater hardness. Genomic assembly comparative analysis ecotype revealed unique metabolism gene cluster, including SmWRKY40 , WRKY transcription factor essential for RA SAB biosynthesis. Overexpression enhanced levels whereas its knockout reduced Integrating high‐throughput (DNA affinity purification sequencing) point‐to‐point (Yeast One‐Hybrid, Dual‐Luciferase Electrophoretic Mobility Shift Assay) protein‐DNA interaction detection platform further identified binding sites across ecotypes, revealing specific networks. Our findings provide insights into molecular basis bioactive compound accumulation, advancing breeding strategies quality improvement S. .

Language: Английский

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The biochemical and molecular mechanisms of plants: a review on insect herbivory

Plant Signaling & Behavior, Journal Year: 2024, Volume and Issue: 20(1)

Published: Dec. 26, 2024

Biochemical and molecular mechanisms have been essential to reduce various insect attacks on plants. The biochemical methods are wide involving direct indirect defenses. defensive chemical substances secreted effectively the wound caused by herbivores (insects phytopathogens) Plants responded producing VOCs which draw natural enemies of insects phytopathogens. progress observed in cognition stimulus plants their potential control responses is characterized modification shifts our attention development endogenous resistance preserving crops. main objective implementing a biotechnological mechanism crop production employ durable multimechanistic alternatives pests via plant produces upon encountering attack.

Language: Английский

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Genome-wide identification and expression analysis of the WRKY gene family in Mikania micrantha

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 3, 2025

WRKY transcription factors (TFs) regulate plant responses to environmental stimuli and development, including flowering. Despite extensive research on different species, their role in the invasive Mikania micrantha remains be explored. The aim of this study was identify analyze genes M. understand function flowering adaptation mechanisms. By analysing whole genome micrantha, a total 77 (MmWRKY) were identified. Based phylogenetic relationships, sequence alignment, structural domain diversity, MmWRKY gene family preliminarily classified into three major groups five subgroups: Group I, II (II-a, II-b, II-c, II-d, II-e), III. Expression profiles showed tissue-specific expression patterns, with many highly expressed flowers, indicating potential roles floral development. Real-time quantitative PCR confirmed that selected 11 tissues, supporting functional significance In study, identified M domains, patterns across various tissues organs comprehensively analyzed. This work provides foundation for future characterization which may contribute development more effective strategies control its rapid spread.

Language: Английский

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Insights into mungbean defense response to Cercospora leaf spot based on transcriptome analysis

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 8, 2025

Several mungbean (Vigna radiata (L.) Wilczek) cultivars are susceptible to Cercospora leaf spot (CLS) caused by canescens Ellis & Martin, and it is necessary explore resistance sources understand mechanisms. However, the CLS mechanisms have not yet been explored. The response revealed significantly different disease severity scores in both genotypes. Hypersensitive (HR) started appear at 2 days after inoculation (DAI) SUPER5 but was never observed CN84-1. exhibited fewer smaller lesions than CN84-1 during infection, resulting being resistant while CLS. In this study, RNA sequencing (RNA-seq) analysis used unravel of a line (SUPER5) variety (CN84-1) upon infection. A total 9510 DEGs including 4615 up-regulated 4895 down-regulated genes were revealed. Of these 3242 1027 uniquely only CN84-1, respectively, 2902 734 respectively. 843 enriched biological processes mainly associated with plant defense responses, fungus, protein phosphorylation chitin Gene Ontology (GO) terms analysis. KEGG pathway showed that represented plant-pathogen interaction, MAPK signaling pathway, hormone signal transduction, cell wall component biosynthesis infection specifically SUPER5. addition, qRT-PCR analyze expression pattern 22 candidate belonging pathogenesis related (PR) proteins, (R) transcription factors, hypersensitive (HR), essential involved activity CLS-infected V. radiata. It found consistent RNA-seq analysis, showing highly significant correlation coefficient 0.7163 (p < 0.01). co-expression network illustrated interactions among genes, which multiple functions response. Interestingly, ones encoding PR-2, thaumatin, peroxidase, defensin, RPM1, pectinesterase, chalcone synthase, auxin efflux carrier, factors (Pti1, Pti5, Pti6 WRKY40) suggesting they might be Moreover, higher β-1,3-glucanase chitinase levels Our findings contribute an understanding may advocate development more effective management approaches.

Language: Английский

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