Exploring the Biological Potential of Mountain Germander Polyphenolic Extract on Cellular Model Macromolecules, Human Cell Lines, and Microbiome Representatives DOI Creative Commons
Ana Mandura Jarić, Ksenija Durgo, Ana Huđek

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(22), P. 10602 - 10602

Published: Nov. 17, 2024

In the context of revitalizing use traditional plant species as remarkable sources bioactive compounds, determination their biological effects is utmost importance. Among Lamiaceae species, Teucrium montanum (Mountain Germander) represents understudied Mediterranean species; it rich in polyphenols, which are well-studied biologically active compounds for human disease prevention and reduction oxidative stress, i.e., phenolic acids, phenylethanoid glycosides, flavonoids. For that purpose, aim this study was to investigate antioxidant, cytotoxic, genotoxic Mountain Germander (MG) polyphenolic extract (0.025, 0.050, 0.150, 0.500 mg mL−1) on hepatocellular (HepG2), tongue (CAL 27), gastric (AGS), colorectal (Caco-2) continuous cancer cell lines, well its bacteriostatic potential representative members microbiota. addition, antioxidant MG determined using bovine serum album DNA plasmid cellular model macromolecules. vitro analysis revealed a significant cytotoxic effect all concentrations AGS Caco-2 lines after prolonged treatment (24 h). with mL−1 showed most pronounced under h) CAL 27 HepG2 lines. All applied seem have genoprotective plasmid. Furthermore, inhibitory E. coli detected upon 0.150 mL−1, reducing viability by 56%.

Language: Английский

Dual Role of Baimao-Longdan-Congrong-Fang in Inhibiting Staphylococcus aureus Virulence Factors and Regulating TNF-α/TNFR1/NF-κB/MMP9 Axis DOI
Tao Jiang, Xingxin Zhu,

Zixin Yin

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156477 - 156477

Published: Feb. 7, 2025

Language: Английский

Citations

1
The secondary metabolites of the alga-derived fungus Aspergillus niveoglaucus КММ 4176 and their antimicrobial and antibiofilm activities DOI

Nadezhda P. Shlyk,

Ekaterina A. Yurchenko, Elena V. Leshchenko

et al.

The Journal of Antibiotics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

1
Structural and functional insights of sortases and their interactions with antivirulence compounds DOI Creative Commons

Sowmiya Sri Sivaramalingam,

Deepsikha Jothivel,

Deenadayalan Karaiyagowder Govindarajan

et al.

Current Research in Structural Biology, Journal Year: 2024, Volume and Issue: 8, P. 100152 - 100152

Published: Jan. 1, 2024

Sortase proteins play a crucial role as integral membrane in anchoring bacterial surface by recognizing them through Cell-Wall Sorting (CWS) motif and cleaving at specific sites before initiating pilus assembly. Both sortases their substrate are major virulence factors numerous Gram-positive pathogens, making attractive targets for antimicrobial intervention. Recognizing the significance of proteins, comprehensive exploration structural functional characteristics is essential to enhance our understanding assembly diverse bacteria. Therefore, this review article discusses features different classes pilin primarily serving substrates sortase-assembled pili. Moreover, it thoroughly examines molecular-level interactions between inhibitors, providing insights from both perspectives. In essence, will provide contemporary complete sortase pathways various strategies target effectively counteract virulence.

Language: Английский

Citations

5
Echinacoside reduces intracellular c-di-GMP levels and potentiates tobramycin activity against Pseudomonas aeruginosa biofilm aggregates DOI Creative Commons
Yu-Ming Cai, Feng Hong,

Amber De Craemer

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 7, 2025

Abstract Cyclic diguanylate (c-di-GMP) is a central biofilm regulator in Pseudomonas aeruginosa , where increased intracellular levels promote formation and antibiotic tolerance. Targeting the c-di-GMP network may be promising anti-biofilm approach, but most strategies studied so far aimed at eliminating surface-attached biofilms, while vivo P. biofilms often occur as suspended aggregates. Here, expression profile of metabolism-related genes was analysed among 32 strains grown aggregates synthetic cystic fibrosis sputum. The cyclase SiaD proved essential for auto-aggregation under vivo-like conditions. Virtual screening predicted high binding affinity echinacoside towards active site SiaD. Echinacoside reduced aggregate sizes potentiated tobramycin activity against >80% tested. This synergism also observed -infected 3-D alveolar epithelial cells murine lungs, demonstrating echinacoside’s potential an adjunctive therapy recalcitrant infections.

Language: Английский

Citations

0
Covalent sortase A inhibitor, α, β-unsaturated aldehydes, prevents Staphylococcus aureus infection DOI
J. Wang,

He Yuan,

Chen-Liang Chu

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107472 - 107472

Published: March 1, 2025

Language: Английский

Citations

0
Epicatechin gallate and its analogues interact with sortase A and β-lactamase to suppress Staphylococcus aureus virulence DOI Creative Commons
Fei Teng, Lihui Wang, Jian Wen

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 25, 2025

Staphylococcus aureus sortase A can anchor virulence proteins, which are responsible for bacterial adhesion, biofilm formation, and inflammation, to the cell membrane surface. The ability of β-lactam antibiotics combat S. infections is limited by presence β-lactamases in this pathogen. In study, we determined that epicatechin gallate (ECG) its analogues inhibited transpeptidase activity interacting with it directly, formation adhesion abilities bacterium decreased after treatment ECG analogues. Additionally, bound β-lactamase reduced hydrolyze nitrocefin. Furthermore, synergized ampicillin (Amp), enhancing bactericidal effects inhibiting persisters. did not affect expression or but significantly alleviated cytotoxicity USA300. alone combined Amp vivo improved survival mice infected USA300, pathological tissue damage pulmonary edema, extent inflammation level colonization. results study indicate active ingredients green tea, especially ECG, have potential be developed as anti- infection agents.

Language: Английский

Citations

0
Butyrate-Producing Mixed Probiotics Alleviate MRSA-Induced Acute Lung Injury in Mice by Promoting M2 Macrophage Polarization DOI
Minghan Li,

Tianxu Pan,

Juntong Yu

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Language: Английский

Citations

0
Identification of echinacoside as a tobramycin potentiator againstPseudomonas aeruginosaaggregates DOI Open Access
Yu-Ming Cai, Aurélie Crabbé, Tom Coenye

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 9, 2024

Abstract Cyclic diguanylate (c-di-GMP) is a central biofilm regulator, where increased intracellular levels promote formation and antibiotic tolerance. Targeting the c-di-GMP network promising anti-biofilm approach. Most agents reported previously decreased to eliminate surface-attached biofilms, which did not recapitulate in vivo biofilms well may thus impede their clinical impact. Here, expression profile of genes encoding proteins associated with metabolism was analysed among 32 Pseudomonas aeruginosa strains grown as suspended aggregates synthetic sputum or planktonic cells. A cyclase, SiaD, proved essential for auto-aggregation under -like conditions. Virtual screening against SiaD identified echinacoside an inhibitor, reduced aggregate sizes potentiated efficacy tobramycin established by >80% tested strains. This synergistic effect also observed 3-D alveolar cells infected cytotoxic P. , demonstrating its high potential adjunctive therapy recalcitrant infections.

Language: Английский

Citations

0
The influence of marine fungal meroterpenoid meroantarctine A toward HaCaT keratinocytes infected with Staphylococcus aureus DOI
Ekaterina A. Chingizova,

Artur R. Chingizov,

Ekaterina S. Menchinskaya

et al.

The Journal of Antibiotics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Language: Английский

Citations

0
Exploring the Biological Potential of Mountain Germander Polyphenolic Extract on Cellular Model Macromolecules, Human Cell Lines, and Microbiome Representatives DOI Creative Commons
Ana Mandura Jarić, Ksenija Durgo, Ana Huđek

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(22), P. 10602 - 10602

Published: Nov. 17, 2024

In the context of revitalizing use traditional plant species as remarkable sources bioactive compounds, determination their biological effects is utmost importance. Among Lamiaceae species, Teucrium montanum (Mountain Germander) represents understudied Mediterranean species; it rich in polyphenols, which are well-studied biologically active compounds for human disease prevention and reduction oxidative stress, i.e., phenolic acids, phenylethanoid glycosides, flavonoids. For that purpose, aim this study was to investigate antioxidant, cytotoxic, genotoxic Mountain Germander (MG) polyphenolic extract (0.025, 0.050, 0.150, 0.500 mg mL−1) on hepatocellular (HepG2), tongue (CAL 27), gastric (AGS), colorectal (Caco-2) continuous cancer cell lines, well its bacteriostatic potential representative members microbiota. addition, antioxidant MG determined using bovine serum album DNA plasmid cellular model macromolecules. vitro analysis revealed a significant cytotoxic effect all concentrations AGS Caco-2 lines after prolonged treatment (24 h). with mL−1 showed most pronounced under h) CAL 27 HepG2 lines. All applied seem have genoprotective plasmid. Furthermore, inhibitory E. coli detected upon 0.150 mL−1, reducing viability by 56%.

Language: Английский

Citations

0