Cited by taxmyPHAGE: Automated taxonomy of dsDNA phage genomes at the genus and species level

Current Clinical Landscape and Global Potential of Bacteriophage Therapy DOI

Nicole Hitchcock, Danielle Devequi Gomes Nunes, Job Shiach

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(4), P. 1020 - 1020

Published: April 21, 2023

In response to the global spread of antimicrobial resistance, there is an increased demand for novel and innovative antimicrobials. Bacteriophages have been known their potential clinical utility in lysing bacteria almost a century. Social pressures concomitant introduction antibiotics mid-1900s hindered widespread adoption these naturally occurring bactericides. Recently, however, phage therapy has re-emerged as promising strategy combatting resistance. A unique mechanism action cost-effective production promotes phages ideal solution addressing antibiotic-resistant bacterial infections, particularly lower- middle-income countries. As number phage-related research labs worldwide continues grow, it will be increasingly important encourage expansion well-developed trials, standardization storage cocktails, advancement international collaboration. this review, we discuss history, benefits, limitations bacteriophage its current role setting resistance with specific focus on active trials case reports administration.

Language: Английский

Citations

Megataxonomy and global ecology of the virosphere DOI

Eugene V. Koonin, Jens H. Kuhn, Valerian V. Dolja

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Nearly all organisms are hosts to multiple viruses that collectively appear be the most abundant biological entities in biosphere. With recent advances metagenomics and metatranscriptomics, known diversity of substantially expanded. Comparative analysis these using advanced computational methods culminated reconstruction evolution major groups enabled construction a virus megataxonomy, which has been formally adopted by International Committee on Taxonomy Viruses. This comprehensive taxonomy consists six realms, aspired monophyletic assembled based conservation hallmark proteins involved capsid structure formation or genome replication. The different taxa differ host range accordingly ecological niches. In this review article, we outline latest developments megataxonomy discoveries will likely lead reassessment some taxa, particular, split three current realms into two more independent realms. We then discuss correspondence between distribution among niches, as well abundance versus cells habitats. across environments appears primarily determined ranges, i.e. virome is shaped composition biome given habitat, itself affected abiotic factors.

Language: Английский

Citations

Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2024) DOI

Peter Simmonds, Evelien M. Adriaenssens, Elliot J. Lefkowitz

et al.

Archives of Virology, Journal Year: 2024, Volume and Issue: 169(11)

Published: Nov. 1, 2024

Abstract This article reports changes to virus taxonomy and taxon nomenclature that were approved ratified by the International Committee on Taxonomy of Viruses (ICTV) in April 2024. The entire ICTV membership was invited vote 203 taxonomic proposals had been Executive (EC) July 2023 at 55th EC meeting Jena, Germany, or second November 2023. All online vote. Taxonomic additions include one new phylum ( Ambiviricota ), class, nine orders, three suborders, 51 families, 18 subfamilies, 820 genera, 3547 species (excluding taxa have abolished). Proposals complete process name replacement binomial (genus + epithet) format ratified. Currently, a total 14,690 established.

Language: Английский

Citations

Bacteriophage therapy for drug-resistant Staphylococcus aureus infections DOI

Kaixin Liu, Chao Wang, Xudong Zhou

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 31, 2024

Drug-resistant Staphylococcus aureus stands as a prominent pathogen in nosocomial and community-acquired infections, capable of inciting various infections at different sites patients. This includes bacteremia (SaB), which exhibits severe infection frequently associated with significant mortality rate approximately 25%. In the absence better alternative therapies, antibiotics is still main approach for treating infections. However, excessive use has, turn, led to an increase antimicrobial resistance. Hence, it imperative that new strategies are developed control drug-resistant S. Bacteriophages viruses ability infect bacteria. Bacteriophages, were used treat bacterial before advent antibiotics, but subsequently replaced by due limited theoretical understanding inefficient preparation processes time. Recently, phages have attracted attention many researchers again because serious problem antibiotic article provides comprehensive overview phage biology, animal models, diverse clinical case treatments, trials context therapy. It also assesses strengths limitations therapy outlines future prospects research directions. review expected offer valuable insights engaged phage-based treatments

Language: Английский

Citations

“Sichuanvirus”, a novel bacteriophage viral genus, able to lyse carbapenem-resistant Klebsiella pneumoniae DOI

Juan Li, Qingqing Fang,

Huan Luo

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 13, 2025

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a severe threat for human health and urgently needs new therapeutic approaches. Lytic bacteriophages (phages) are promising clinically viable options against CRKP. We attempted to isolate lytic phages CRKP of sequence type 11 capsular 64 (ST11-KL64), the predominant in China. recovered phage from sewage collected at wastewater treatment station Sichuan province, obtained genome this found that it distinct all known with highest overall DNA similarity (12.5%, 16% coverage 78.4% identity) vB_EcoM_PHB05 (accession no. NC_052652) ICTV. This represents novel viral genus subfamily Stephanstirmvirinae, which we proposed "Sichuanvirus" as name. has narrow host range lyse specific KL64 Klebsiella. no genes referring antimicrobial resistance, virulence, lysogen stable wide pH temperatures. also three bacterial mutants resistant performed sequencing them. therefore discovered interruption polysaccharide biosynthesis-related gene wcaJ by insertion sequences mediated resistance phage. characterized "Sichuanvirus", suitable therapy. The discovery expands arsenal

Language: Английский

Citations

taxMyPhage: Automated Taxonomy of dsDNA Phage Genomes at the Genus and Species Level DOI

Andrew Millard, Rémi Denise,

Maria Lestido

et al.

PHAGE, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Background: Bacteriophages are classified into genera and species based on genomic similarity, a process regulated by the International Committee Taxonomy of Viruses. With rapid increase in phage data there is growing need for automated classification systems that can handle large-scale genome analyses place phages new or existing species.

Language: Английский

Citations

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns DOI

Amr Abd El‐Wahab, Shereen Basiouni, Hesham R. El‐Seedi

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 21, 2023

The primary contaminants in poultry are Salmonella enterica , Campylobacter j ejun i, Escherichia coli, and Staphylococcus aureus . Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses poses a threat public health. With increasing prevalence bacterial pathogens being resistant most conventional antibiotics, scientists have rekindled interest using bacteriophages as antimicrobial agents. Bacteriophage treatments also been investigated an alternative antibiotics industry. Bacteriophages’ high specificity may allow them only target specific pathogen infected animal. However, tailor-made sophisticated cocktail different could broaden their antibacterial activity typical situations multiple clinical strains infections. Bacteriophages not be used terms reducing contamination animals but also, under industrial conditions, they can safe disinfectants reduce on food-contact surfaces or carcasses. Nevertheless, bacteriophage therapies developed sufficiently for use. Problems resistance, safety, specificity, long-term stability must addressed particular. This review highlights benefits, challenges, current limitations applications

Language: Английский

Citations

Characterization of crAss-like phage isolates highlights Crassvirales genetic heterogeneity and worldwide distribution DOI

María Dolores Ramos‐Barbero, Clara Gómez-Gómez, Laura Sala‐Comorera

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 18, 2023

Crassvirales (crAss-like phages) are an abundant group of human gut-specific bacteriophages discovered in silico. The use crAss-like phages as fecal indicators is proposed but the isolation only seven cultured strains to date has greatly hindered their study. Here, we report and genetic characterization 25 new (termed crAssBcn) infecting Bacteroides intestinalis, belonging order Crassvirales, genus Kehishuvirus and, based on genomic variability, classified into six species. CrAssBcn phage genomes similar ΦCrAss001 show aminoacidic differences when compared other same family. detected metagenomes around world at a higher frequency than ΦCrAss001. This study increases known isolates abundance heterogeneity open question what member should be selected marker.

Language: Английский

Citations

Acinetobacter Baumannii Phages: Past, Present and Future DOI

Qihang Tu,

Mingfang Pu,

Yahao Li

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(3), P. 673 - 673

Published: March 3, 2023

Acinetobacter baumannii (A. baumannii) is one of the most common clinical pathogens and a typical multi-drug resistant (MDR) bacterium. With increase drug-resistant A. infections, it urgent to find some new treatment strategies, such as phage therapy. In this paper, we described different drug resistances basic properties phages, analyzed interaction between phages their hosts, focused on therapies. Finally, discussed chance challenge This paper aims provide more comprehensive understanding theoretical support for application phages.

Language: Английский

Citations

Phage fibers and spikes: a nanoscale Swiss army knife for host infection DOI

Ruochen Ouyang, Véronique Ongenae, Alise R. Muok

et al.

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 77, P. 102429 - 102429

Published: Jan. 25, 2024

Bacteriophages are being rediscovered as potent agents for medical and industrial applications. However, finding a suitable phage relies on numerous factors, including host specificity, burst size, infection cycle. The range of is, besides defense systems, initially determined by the recognition attachment receptor-binding proteins (RBPs) to target receptors susceptible bacteria. RBPs include tail (or occasionally head) fibers tailspikes. Owing potential flexibility heterogeneity these structures, they often overlooked during structural studies. Recent advances in cryo-electron microscopy studies computational approaches have begun unravel their fundamental mechanisms infection. In this review, we discuss current state research different head fibers, spike models, molecular mechanisms. These details may facilitate manipulation phage-host which turn will important implications science society.

Language: Английский

Citations