Isolation and genome-wide analysis of new Acinetobacter baumannii bacteriophage vB_AbaM_AB3P2 DOI Creative Commons
Yujing Tan,

Jianhui Su,

Dandan Luo

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 25, 2023

Abstract New lytic Acinetobacter baumannii phage, vB_AbaM_AB3P2, was isolated from sewage treatment plant of Luoyang, China. The genome vB_AbaM_AB3P2 is composed double-strand DNA that 44,824bp in length with G + C content 37.75%. 96 open reading frames were identified, genes for antibiotic resistance and virulence factors not found. genomic phylogenetic analyses this phage revealed it represents a new species genus Obolenskvirus. Phage which has short latent period (10 min) high stability 30–70 ◦C pH 2–10, potentially useful controlling multi-drug resistant A. the future.

Language: Английский

Unveiling the Secrets of Acinetobacter baumannii: Resistance, Current Treatments, and Future Innovations DOI Open Access
Andrea Marıno, Egle Augello, Stefano Stracquadanio

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6814 - 6814

Published: June 21, 2024

represents a significant concern in nosocomial settings, particularly critically ill patients who are forced to remain hospital for extended periods. The challenge of managing and preventing this organism is further compounded by its increasing ability develop resistance due extraordinary genomic plasticity, response adverse environmental conditions. Its recognition as public health risk has provided impetus the identification new therapeutic approaches infection control strategies. Indeed, currently used antimicrobial agents gradually losing their efficacy, neutralized newer mechanisms bacterial resistance, especially carbapenem antibiotics. A deep understanding underlying molecular urgently needed shed light on properties that allow

Language: Английский

Citations

16
Phage therapy: an alternative treatment modality for MDR bacterial infections DOI

Namrata Pal,

Poonam Sharma, Manoj Kumawat

et al.

Infectious Diseases, Journal Year: 2024, Volume and Issue: 56(10), P. 785 - 817

Published: July 17, 2024

The increasing global incidence of multidrug-resistant (MDR) bacterial infections threatens public health and compromises various aspects modern medicine. Recognising the urgency this issue, World Health Organisation has prioritised development novel antimicrobials to combat ESKAPEE pathogens. Comprising

Language: Английский

Citations

12
Discovery, structural characteristics and evolutionary analyses of functional domains in Acinetobacter baumannii phage tail fiber/spike proteins DOI Creative Commons

Shenshen Liu,

Tao Lei, Yujing Tan

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 12, 2025

Abstract Background The global rise in multidrug-resistant Acinetobacter baumannii infections poses a significant healthcare challenge. Bacteriophage offer promising alternative to antibiotics for treating A. infections. Phage tail fiber and spike proteins are essential host recognition, with some exhibiting depolymerase activity that aids degrading the bacterial cell wall, facilitating infection. Detailed studies of functional domains responsible receptor-binding phage fiber/spike crucial step toward developing effective treatments. Results A total 32 were identified across 313 from 204 publicly available phages using InterPro AlphaFold3. Domains associated function Pectin lyase-like domain (PLD), phage_tailspike_middle (PTMD), Transglycosidases (TGD), SGNH hydrolase (SHD). These primarily found Autographiviridae family, specifically within Friunavirus genus. predominant PLD displayed high variability, its sequence conserved only 25-amino-acid region among two closely related protein lineages. All enzymatic exhibit diversity yet retain structural stability, which is function. As domains, four types pyocin_knob (PKD) initially identified, characterized by unique β-sheet α-helix configurations. Each type PKD exhibited distinct potential sites, located region, was Obolenskvirus genus, as well Straboviridae families. G3DSA:2.60.40.3940 domain, minor variations, predominantly Additionally, novel Obo-β-sandwich structure, discovered genus cluster. these accounts their interactions various receptors. Conclusions This research deepens understanding relationship between genera proteins, emphasizing compatibility characteristics roles. data obtained could serve reference targeted modification or enhancing therapeutic applications.

Language: Английский

Citations

2
Isolation and Characterization of Novel Bacteriophages to Target Carbapenem-Resistant Acinetobacter baumannii DOI Creative Commons
Yoon-Jung Choi, Shukho Kim, Minsang Shin

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(7), P. 610 - 610

Published: June 29, 2024

The spread of multidrug-resistant Acinetobacter baumannii in hospitals and nursing homes poses serious healthcare challenges. Therefore, we aimed to isolate characterize lytic bacteriophages targeting carbapenem-resistant (CRAB). Of the 21 isolated A. phages, 11 exhibited potent activities against clinical isolates CRAB. Based on host spectrum RAPD-PCR results, phages were categorized into four groups. Three (vB_AbaP_W8, vB_AbaSi_W9, vB_AbaSt_W16) further characterized owing their antibacterial efficacy, morphology, whole-genome sequence found lyse 37.93%, 89.66%, respectively, 29 tested CRAB isolates. varied depending multilocus type (MLST) three contained linear double-stranded DNA genomes, with sizes 41,326–166,741 bp GC contents 34.4–35.6%. Genome-wide phylogenetic analysis single gene-based tree construction revealed no correlation among phages. Moreover, genes associated lysogeny, antibiotic resistance, or bacterial toxins. novel represent potential candidates for phage therapy infections.

Language: Английский

Citations

9
Phage therapy: A renewed approach against oral diseases caused by Enterococcus faecalis infections DOI
Dan Yang, Yingying Xiang, Fei Song

et al.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 189, P. 106574 - 106574

Published: Feb. 13, 2024

Language: Английский

Citations

8
Synergistic Antimicrobial Effects of Phage vB_AbaSi_W9 and Antibiotics against Acinetobacter baumannii Infection DOI Creative Commons
Yoon-Jung Choi, Shukho Kim, Minsang Shin

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(7), P. 680 - 680

Published: July 22, 2024

Acinetobacter baumannii is a challenging multidrug-resistant pathogen in healthcare. Phage vB_AbaSi_W9 (GenBank: PP146379.1), identified our previous study, shows lytic activity against 26 (89.66%) of 29 carbapenem-resistant (CRAB) strains with various sequence types (STs). It promising candidate for CRAB treatment; however, its efficiency insufficient complete bacterial lysis. Therefore, this study aimed to investigate the clinical utility phage by identifying antimicrobial agents that show synergistic effects when combined it. The A. ATCC17978 strain was used as host vB_AbaSi_W9. Adsorption and one-step growth assays were performed at MOIs 0.001 0.01, respectively. Four belonging different types, KBN10P04948 (ST191), LIS2013230 (ST208), KBN10P05982 (ST369), KBN10P05231 (ST451), phage-antibiotic synergy. Five antibiotics tested following concentration: meropenem (0.25-512 µg/mL); colistin, tigecycline, rifampicin (0.25-256 ampicillin/sulbactam (0.25/0.125-512/256 µg/mL). vitro effect verified through an vivo mouse infection model. demonstrated 90% adsorption cells 1 min, 20 min latent period, burst size 114 PFU/cell. Experiments combining pronounced tigecycline rifampicin. In model infected group treated (100 μg/mL) (MOI 1) achieved 100% survival rate-a significant improvement over phage-only treatment (8.3% rate) or antibiotic-only (25% groups. bacteriophage excellent synergy rifampicin, suggesting potential candidates combination therapy treating infections.

Language: Английский

Citations

7
Recent insights on phage therapy against multidrug-resistant Acinetobacter baumannii DOI Creative Commons
Ann A. Elshamy, Salwa M. Kamal, Mohamed Basyony

et al.

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 12, 2025

Abstract Acinetobacter baumannii is a prevalent clinical pathogen commonly found to be multidrug-resistant (MDR), causing serious life-threatening infections, particularly hospital-acquired infections with limited therapeutic options. The MDR phenotype developed against this critical increasingly globally, reaching pan-drug-resistant conferring non-susceptibility all antimicrobials used in its treatment according the standard guidelines. Therefore, it develop innovative approaches, such as phage therapy, considering rise drug-resistant A. infections. In review, we highlight and discuss up-to-date antimicrobial resistance of , use phages, their limitations, future perspectives treating addition, combination phages antimicrobials, preclinical studies including pharmacokinetics pharmacodynamics properties have been discussed.

Language: Английский

Citations

1
Bayesian Evaluation of Phage Therapy Efficacy Against Multidrug-Resistant Acinetobacter baumannii DOI Creative Commons

Mohsin Malik,

Sobia Manzoor, Javed Ashraf

et al.

International Journal of Antimicrobial Agents, Journal Year: 2025, Volume and Issue: unknown, P. 107508 - 107508

Published: April 1, 2025

Language: Английский

Citations

1
Structural diversity among Acinetobacter baumannii K-antigens and its implication in the in silico serotyping DOI Creative Commons

Janardhanaachari Roshini,

L. Ponoop Prasad Patro, Sruthi Sundaresan

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: June 21, 2023

Acinetobacter baumannii is an emerging opportunistic pathogen. It exhibits multi-, extreme-, and pan-drug resistance against several classes of antibiotics. Capsular polysaccharide (CPS or K-antigen) one the major virulence factors which aids A. in evading host immune system. K-antigens exploit Wzx/Wzy-dependent pathway that involves 13 different proteins for its assembly transport onto outer membrane. A total 64 (out 237 K-locus(KL) types) known K-antigen sugar repeating structures are discussed here classified into seven groups based on their initial sugars, QuiNAc4NAc, GalNAc, GlcNAc, Gal, QuiNAc/FucNAc, FucNAc, GlcNAc along with Leg5Ac7Ac/Leg5Ac7R. Thus, corresponding initializing glycosyltransferases (ItrA1, ItrA2, ItrA3, ItrA4, ItrB1, ItrB3, ItrA3 ItrB2) exhibit serotype specificity. The modeled 3D-structural repository can be accessed at https://project.iith.ac.in/ABSD/k_antigen.html . topology further reveals presence 2-6 0-4 monomers main side chains, respectively. negatively (predominant) neutrally charged observed Such diversity composition provides K-typing specificity ( viz ., 18–69% terms reliability) Wza, Wzb, Wzc, Wzx, Wzy involved pathway. Interestingly, degree uniqueness these among K-types estimated to 76.79%, considering reference sequences. This article summarizes structural creation a digital systematic analysis transportation marker proteins.

Language: Английский

Citations

15
Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii DOI Creative Commons

Paloma Aparecida Alves de Oliveira,

Juliana Baboghlian,

Clarissa Orandina Aparecida Ramos

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 15, 2024

Abstract Acinetobacter baumannii is a Gram-negative bacterium considered an emerging multi-drug-resistant pathogen. Furthermore, this can survive in extreme environmental conditions, which makes it frequent cause of nosocomial infection outbreaks. Gene expression analyses by Reverse Transcription Quantitative real-time PCR (RT-qPCR) depend on reference gene, also called endogenous used to normalize the generated data and thus ensure accurate analysis with minimal errors. Currently, gene A. are compromised, as there no reports literature describing identification validated genes for use RT-qPCR analyses. For reason, we selected twelve candidate assessed their profile under different experimental culture conditions. The stability was evaluated using statistical algorithms such BestKeeper, geNorm, NormFinder, Delta C T , RefFinder, order identify most suitable indicated rpoB rpoD fabD adequate normalization . accuracy proposed them ompA encoding outer membrane protein A, sensible resistant antibiotic polymyxin. present work provides precise future studies

Language: Английский

Citations

5