Genome-wide DNA methylation profiling reveals a novel hypermethylated biomarker PRKCB in gastric cancer DOI Creative Commons

Leyan Li,

Fei Xiao, Huan Wang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 4, 2024

Globally, gastric cancer (GC) ranks among the most prevalent forms of malignancy, posing a significant health burden. Epigenetic modifications, predominantly characterized by alterations in DNA methylation patterns, have been linked to diverse array neoplastic processes. Here, we undertake comprehensive analysis signature GC, with aim discover potential diagnostic epigenetic biomarkers. Utilizing Illumina 935 K BeadChip, conducted genome-wide exploration patterns four paired samples GC tissues and adjacent non-cancerous counterparts. The bisulfite-pyrosequencing (n = 7) was employed quantification for methylated gene. pubic databases including GWAS Catalog, TCGA GEO were used. immunohistochemistry qRT-PCR performed. In contrast tissues, manifested pronounced hypermethylation specifically within promoter cytosine-phosphate-guanine (CpG) islands, indicating localized alterations. methylome further revealed 4432 differentially-methylated probes (DMPs), gene PRKCB exhibited prominent average disparity (mean Δβ 0.353). Pyrosequencing validation confirmed three DMPs (cg08406370, cg00735962, cg18526361). Notably, mean levels inversely correlated mRNA expression Catalog. Furthermore, both protein significantly reduced GCs when compared their counterparts, verified database. Our study reveals emphasizes pivotal role conferring risk, which offers fresh perspectives advancing approaches therapeutic strategies GC.

Language: Английский

Exploring the Anti-Colon Cancer Potential of Polygonum minus: Integrating In Vitro and In Silico Studies DOI
Zhongming Yang, Zhongguo Yang,

Md Zuki Abu Bakar

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: 64, P. 105853 - 105853

Published: Jan. 6, 2025

Language: Английский

Citations

3
Erythronecroptosis: an overview of necroptosis or programmed necrosis in red blood cells DOI
Anton Tkachenko, Ondřej Havránek

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: 479(12), P. 3273 - 3291

Published: March 1, 2024

Language: Английский

Citations

11
Discovery of Noncovalent Diaminopyrimidine-based Inhibitors for Glioblastoma via a Dual FAK/DNA Targeting Strategy DOI
Yi Wei, Xiaoling He,

Zhiwu Long

et al.

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 286, P. 117288 - 117288

Published: Jan. 16, 2025

Language: Английский

Citations

1
Cell death signaling in human erythron: erythrocytes lose the complexity of cell death machinery upon maturation DOI Creative Commons
Anton Tkachenko, Ondřej Havránek

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Abstract Over the recent years, our understanding of cell death machinery mature erythrocytes has been greatly expanded. It resulted in discovery several regulated (RCD) pathways red blood cells. Apoptosis (eryptosis) and necroptosis share certain features with their counterparts nucleated cells, but they are also critically different particular details. In this review article, we summarize subroutines erythroid precursors (apoptosis, necroptosis, ferroptosis) comparison to (eryptosis erythronecroptosis) highlight consequences organelle clearance associated loss multiple components upon erythrocyte maturation. Recent advances role RCDs health disease have expanded potential clinical applications these lethal subroutines, emphasizing contribution development anemia, microthrombosis, endothelial dysfunction, as well diagnostic biomarkers markers storage-induced lesions. Fas signaling functional caspase-8/caspase-3 system not indispensable for eryptosis, might be retained mediate crosstalk between both erythrocyte-associated RCDs. The ability switch eryptosis suggests that is a simple unregulated mechanical disintegration, tightly controlled process. This allows investigation eventual pharmacological interventions aimed at individual erythrocytes.

Language: Английский

Citations

1
Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review) DOI
Anton Tkachenko

Toxicology in Vitro, Journal Year: 2024, Volume and Issue: 98, P. 105814 - 105814

Published: April 4, 2024

Language: Английский

Citations

8
Effects of selenium and iodine on Kashin-Beck disease: an updated review DOI Creative Commons
Lin Liu, Pan Luo, Pengfei Wen

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: May 2, 2024

Kashin-Beck disease (KBD) is an endochondral osteogenesis disorder characterised by epiphysis damage and secondary deformable arthropathy induced multiple external factors, among which selenium (Se) iodine deficiency are important influencing factors. Iodine usually accompanied a low Se content in the soil KBD areas of China. can reverse oxidative to chondrocytes. In addition, related bone conversion rate mineral density. Low will hinder growth change metabolism, resulting decrease Thyroid hormone imbalance caused thyroid dysfunction homeostasis. Compared with alone, combined reduce activity glutathione peroxidase more effectively, increases vulnerability chondrocytes other target cells stress, chondrocyte death. Clinical studies have shown that supplementation helpful for prevention treatment KBD.

Language: Английский

Citations

4
Toxic Effects and Mechanism of Cadmium-Induced Oxidative Damage in Neuronal Cells DOI

Zhu-Zhong Mei,

Jie Yang, Yuan Zhao

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Molecular mechanisms of berberine’s effects on tumor cells DOI Open Access
С. В. Тимофеева, E. Yu. Zlatnik, Лариса Ващенко

et al.

Kazan medical journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Malignant neoplasms remain one of the leading causes mortality worldwide, underscoring need for development novel and effective therapeutic strategies their treatment. In recent years, scientific community has actively pursued search pharmaceutical preparations with minimal adverse effects high efficacy, capable achieving complete remission in patients. this context, berberine, a natural phytochemical compound derived from various species Berberis, garnered increasing attention due to its diverse pharmacological properties. Berberine, recognized as nutraceutical, exhibits broad spectrum biological activities, including anti-inflammatory, antioxidant, antitumor effects. vitro vivo studies have demonstrated that berberine exerts inhibitory on several types cancer, breast, lung, liver, colorectal cancers. Its properties are associated key molecular mechanisms through which it targets tumor cells. review, we comprehensively examined pathways mediated. Specifically, activates caspase cascade, induction apoptosis cells, inhibits cell proliferation by blocking signaling such PI3K/Akt/mTOR. Additionally, modulates expression genes migration invasion, matrix metalloproteinases E-cadherin, making promising candidate approaches. It is also noteworthy anti-inflammatory properties, may contribute prevention carcinogenesis protecting cells oxidative stress inflammatory processes linked cancer development. These position agent further research clinical application, both monotherapy combination other anticancer drugs. Thus, represents an intriguing subject investigation action potential use oncology, could lead more safer patients malignant neoplasms.

Language: Английский

Citations

0
Mn3O4 Nanocrystal-Induced Eryptosis Features Ca2+ Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes DOI Open Access

Yuriy Kot,

Volodymyr Prokopіuk, Vladimir Klochkov

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3284 - 3284

Published: April 1, 2025

Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed synthesized Mn3O4 NPs, identifying ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility. Concentrations up 20 mg/L were found be safe erythrocytes. Eryptosis assays shown more sensitive than fragility as markers Flow cytometry- confocal microscopy-based studies revealed induced by NPs was accompanied Ca2+ overload, altered homeostasis verified enhanced intracellular reactive oxygen species (ROS) nitrogen (RNS), a decrease lipid order cell membranes. Furthermore, NP-induced calpain- caspase-dependent.

Language: Английский

Citations

0
Synergistic Inhibition of Colon Cancer Cell Proliferation via p53, Bax, and Bcl-2 Modulation by Curcumin and Plumbagin Combination DOI Creative Commons
Iftikhar Ahmad, Sameer Ahmad, Md Abdus Samad

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Cancer is a major contributor to global morbidity and mortality. Among the different forms of cancer, colorectal cancer (CRC) third most frequently diagnosed in men second common type women globally. We aimed explore possible synergistic anticancer potential curcumin (Cur) plumbagin (PL) human colon cell line (HCT-116). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)/cytotoxicity assay revealed IC50 values 7.7 7.5 μM for Cur PL, respectively, as separate entity. However, combined treatment + PL significantly enhanced growth inhibitory compared with solitary treatments an value 6.8 μM. also led induction apoptosis by 41%, cycle arrest at G2/M phase, while Bax p53 genes were found be upregulated Bcl-2 gene was downregulated untreated/solvent control. Furthermore, elevated reactive oxygen species (ROS) production 59% resulted decline mitochondrial membrane (MMP) Catalase superoxide dismutase (SOD) activities reduced, leading lipid peroxidation (LPO) compromised integrity, which confirmed 4',6-diamidino-2-phenylindole (DAPI) propoidium iodide (PI) staining noted. Our vitro data further supported molecular docking, showed higher binding energy proteins (Bax, Bcl-2, p53) PL. Overall, our findings highlight potent effects combination, can exploited combination therapy CRC.

Language: Английский

Citations

0