Exercise training improves diabetic renal injury by reducing fetuin-A, oxidative stress and inflammation in type 2 diabetic rats

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27749 - e27749

Published: March 1, 2024

BackgroundDiabetic kidney disease (DKD) stands as a primary contributor to end-stage renal disease, associated with heightened mortality in cardiovascular diseases. This study aimed explore the impact of an eight-week high-intensity interval training (HIIT) on injury diabetic rats.Methods.Twenty-eight male Wistar rats were randomly allocated into four groups: healthy control (CTL), (DC), exercise (EX), and diabetes-exercise (D + EX). Induction diabetes DC D EX groups occurred through two-month high-fat diet followed by single dose 35 mg/kg streptozotocin (STZ). Rats underwent 4–10 intervals HIIT (80–100% Vmax) over 8 weeks. Subsequently, pathological biochemical parameters assessed serum tissue experimental groups.Results.In group, led elevated damage, glomerulosclerosis, fasting blood glucose (FBG), Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index, animal weight, dysfunction, albuminuria, glomerular filtration rate. Additionally, levels fetuin-A increased, along KIM-1. Mechanistically, induction resulted inflammation elevating tumor necrosis factor-alpha (TNF-α), transforming growth factor beta (TGF-β), interleukin 6 (IL-6), while reducing IL-10 increasing IL-6/IL-10 ratio. Furthermore, triggered oxidative stress, evidenced increased Malondialdehyde (MDA) decreased glutathione peroxidase (GPx), catalase, superoxide dismutase (SOD). mitigated adverse effects group compared group.Conclusion.Our findings suggest that ameliorates type 2 (T2D)-induced damage mitigating inflammation, lowering fetuin-A, bolstering antioxidant defenses. highlights potential time-efficient intervention nephropathy.

Language: Английский

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Lactate-induced autophagy activation: unraveling the therapeutic impact of high-intensity interval training on insulin resistance in type 2 diabetic rats

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 11, 2024

Impaired autophagy is a hallmark of diabetes. The current study proposed to investigate if high intensity interval training (HIIT) induced lactate accumulation could stimulate in type 2 diabetic male rats. 28 Wistar rats were randomly assigned into four groups: Healthy Control (CO), Diabetes (T2D), Exercise (EX), and + (T2D EX). was by feeding high-fat diet administrating single dose streptozotocin (35 mg/kg). After becoming diabetic, the animals exercise groups (EX T2D EX) performed an eight-week HIIT (4-10 interval, 80-100% Vmax, 5 days per week). Serum levels lactate, glucose insulin as well pyruvate, transporter monocarboxylate 1 (MCT1), phosphorylated mitogen-activated protein kinases (p-MAP 2), extracellular signal-regulated (p-ERK mammalian target rapamycin (p-mTOR), ribosomal S6 kinase beta-1 (p-70S6k), p90 (p-90RSK), related 7 (ATG7), Beclin-1, microtubule-associated 1A/1B, 2A/2B -light chain 3 (LC3-I), (LC3- II), (LC3I/LC3II) soleus muscle measured. Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) serum lower EX compared group (P < 0.0001). While not different between Ex, Pyruvate 0.01), MCT1, p-ERK1/2, p-mTOR, p70S6k, P-90RSK, ATG7, LC3-II, LC3-II/LC3I ratios higher We concluded that eight weeks high-intensity activated ERK/P90SRK while inhibiting mTOR/P70S6K signaling pathway dependent manner. It means increased which resulted improve resistance (IR) reduce blood glucose.

Language: Английский

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ATP releasing channels and the ameliorative effects of high intensity interval training on diabetic heart: a multifaceted analysis

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 26, 2024

Abstract Type 2 diabetes (T2D) can cause severe cardiac complications at functional, histologic and molecular levels. These pathological could be mediated by ATP-releasing channels such as Panx1 ATP receptors, in particular P2X7. The aim of our study was to investigate the effect high-intensity interval training (HIIT) on T2D-induced histopathological levels, with a focus channels. 48 male Wistar rats age 8 weeks were randomly allocated into four groups: control (Con), Diabetes (T2D), Training (TR), + (T2D TR). T2D induced high-fat diet plus low dose (35 mg/kg) STZ administration. Rats TR groups underwent an 8-weeks program involving intervals ranging from 80 100% their maximum running speed (Vmax), 4–10 per session. Protein expression Interleukin 1β (IL1β), 10 (IL-10), Pannexin 1 (Panx1), P2X7R (purinergic P2X receptor 7), NLRP1 (NLR Family Pyrin Domain Containing 1), BAX, Bcl2 measured heart tissue. Additionally, we assessed function, changes, well insulin resistance using homeostasis model assessment (HOMA-IR). In contrast group, HIIT led increased protein IL-10 heart. It also resulted improvements systolic diastolic blood pressures, rate, ± dp/dt (maximum minimum changes left ventricular pressure), while reducing IL-1β, Panx1, P2X7R, NLRP1, BAX levels Furthermore, pressure (LVDP) reduced ( P ≤ 0.05). Moreover, lesion scores but decreased HIIT, along reduction fibrosis percentage results this suggest that cardioprotective effects diabetic may modulation This lead inflammation apoptosis, improve attenuate injury fibrosis.

Language: Английский

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Adiponectin receptor 1 could explain the sex differences in molecular basis of cognitive improvements induced by exercise training in type 2 diabetic rats

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 27, 2023

Adipokines dysregulation, the main reason for cognitive impairments (CI) induced by diabetes, shows a sex-dependent pattern inherently and in response to exercise. This study aimed compare attenuating effect of 8-week high intensity-interval training (HIIT) on type 2 diabetes (T2D)-induced CI between male female rats with special focus adiponectin leptin. 28 & Wistar an average age 8 weeks were randomly assigned into four groups: control (Con), exercise (EX), Diabetes (T2D), Type + (T2D Ex). Rats EX T2D groups performed HIIT eight (80-100% Vmax, 4-10 intervals). was months high-fat diet single dose STZ (35 mg/kg) administration. Leptin levels serum measured along hippocampal expression leptin receptors, AMP-activated protein kinase (AMPK), dephosphorylated glycogen synthase kinase-3 beta (Dep-GSK3β), Tau, beta-amyloid (Aβ). Homeostasis model assessments (HOMAs) quantitative insulin-sensitivity check index (QUICKI) indices calculated. Our results showed that following T2D, APN, receptor 1 (APNR1) higher HOMA-IR lower than (P < 0.05). However, after HIIT, APNR1 AMPK as well QUICKI GSK, Aβ females compared While risk more ameliorating animals APN1 player.

Language: Английский

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High Intensity Interval Training can Ameliorate Hypothalamic Appetite Regulation in Male Rats with Type 2 Diabetes: The Role of Leptin

Cellular and Molecular Neurobiology, Journal Year: 2023, Volume and Issue: 43(8), P. 4295 - 4307

Published: Oct. 12, 2023

Language: Английский

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Lactate Mediates Exercise-Induced Modulation of Mitophagy and Ferroptosis, Reducing Amyloid-Beta and Tau in Type 2 Diabetes: A Molecular Study

Published: Jan. 1, 2025

Background: This study examined the impact of lactate accumulation, induced by high-intensity interval training (HIIT), on reducing amyloid-beta (Aβ) and tau protein levels through enhanced mitophagy decreased ferroptosis in hippocampus type 2 diabetic rats.Methods: Thirty-two male Wistar rats were divided into four groups: control (Co), exercise (EX), diabetes (DB), with (DB + EX). Diabetes was DB EX groups via a high-fat diet followed streptozotocin injection (35 mg/kg). The performed treadmill-based HIIT, involving 4–10 running intervals at 80–100% Vmax. Serum hippocampal expression MCT2, SIRT1, BDNF, p62, Keap1, NRF2, MDA, GPX4, PINK1, parkin, Aβ, Tau assessed.Results: In group, Parkin elevated, while reduced compared to group.Conclusion: HIIT enhances reduces lactate-SIRT1-BDNF p62-Keap1-NRF2 pathways, potentially mitigating Aβ accumulation.

Language: Английский

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Potential Role of High‐Intensity Interval Training‐Induced Increase in Humanin Levels for the Management of Type 2 Diabetes

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(3)

Published: Feb. 1, 2025

ABSTRACT This study investigated the effect of 8 weeks high‐intensity interval training (HIIT) on oxidative stress, inflammation, and apoptosis in rats with type 2 diabetes (T2D), focusing role Humanin (HN). In this study, 28 male Wistar were assigned to one four groups: healthy control (CO), exercise (EX), + (T2D EX). After induction (2‐month high‐fat diet injection 35 mg/kg streptozotocin), animals EX T2D groups underwent an 8‐week HIIT protocol (4–10, 80%–100% maximum speed). HOMA‐IR, fasting blood glucose, HN levels measured serum. The expression HN, Bax, Bcl‐2, CAT, GPx, MDA, TNFα, IL‐10 was soleus muscle. Our results showed that serum level muscle IL‐10, SOD, Bax higher group than group. However, HOMA‐IR index Bcl‐2 lower Muscle GPx no significant difference between groups. result Pearson analysis a correlation Bcl‐2. provides evidence there is HIIT. benefits by reducing inflammation stress. Given Humanin's established involvement it possible are mediated humanin.

Language: Английский

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The Impact of Exercise Training on the Brain and Cognition in Type 2 Diabetes, and its Physiological Mediators: A Systematic Review

Sports Medicine - Open, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 24, 2025

Abstract Background Type 2 diabetes (T2DM) affects brain structure and function, is associated with an increased risk of dementia mild cognitive impairment. It known that exercise training has a beneficial effect on cognition at least in healthy people, but the impact these aspects remains to be fully elucidated patients T2DM. Objective To determine function T2DM, identify involved physiological mediators. Methods This paper systematically reviews studies evaluate aims indicate most modality for improving or preserving this patient group. In addition, possible mediators targets improvements are narratively described second part review. Papers published up until 14th January 2025 were searched by means electronic databases PubMed, Embase, Web Science. Studies directly investigating any kind animal models thereof, included, exception human assessing only one time point, combining other interventions (e.g. dietary changes, training, etc.). Study quality was assessed TESTEX tool studies, CAMARADES studies. Results For systematic review, 22 papers found eligible. 18 out (81.8%) showed significant positive which two minority tests. Four (18.2%) could not find Resistance endurance equally effective achieving improvement. Machine-based power seemingly more than resistance body weight elastic bands reach BDNF, lactate, leptin, adiponectin, GSK3β, GLP-1, AMPK/SIRT1 pathway, PI3K/Akt pathway identified as plausible from Via mediators, induces multiple such neuroplasticity, insulin sensitivity, decreased inflammation. Conclusion Overall, beneficially having similar effects. However, there need additional methodological consistency between different order define program optimal Furthermore, we able several further research necessary unravel entire process.

Language: Английский

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Effects of high-intensity interval training on metabolic impairments in liver tissue of rats with type 2 diabetes: a metabolomics-based approach

Journal of Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

Language: Английский

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Reduction in Hippocampal Aβ Content During Gly-Pro-Glu Co-Administration Is Associated with Changes in Inflammation and IGF-I Signaling

Published: April 18, 2024

Alzheimer´s disease (AD) is characterized by the brain deposition of senile plaques composed amyloid-β peptides (Aβ) that increase inflammation. An endogenous peptide derived from insulin-like growth factor (IGF)-I, glycine-proline-glutamate (GPE) has IGF-I-sensitizing and neuroprotective actions. Here, we examined effects GPE on Aβ levels hippocampal inflammation generated intracerebroventricular infusion Aβ25-35 during 2 weeks (300 pmol/day) in ovariectomized rats signaling-related pathways Aβ-degrading enzymes associated with these GPE-related effects. prevented Aβ-induced phosphorylation p38 mitogen-activated protein kinase reduction activation signal transducer activator transcription 3, insulin receptor substrate-1 Akt, as well interleukin (IL)-2 IL-13 hippocampus. The functionality somatostatin, measured percentage inhibition adenylate cyclase activity insulin-degrading enzyme were also preserved co-treatment. These findings indicate co-administration may protect insult changing cytokine content somatostatin through regulation leptin- IGF-I-signaling could influence modulation and/or proteases.

Language: Английский

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