Mendelian randomization analyses of genetically predicted circulating levels of cytokines with risk of Inflammatory bowel disease DOI Creative Commons
Xianqiang Liu, Dingchang Li, Wenxing Gao

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 12, 2023

Abstract Objective The literature has previously reported the associations between inflammatory bowel disease (IBD) and certain cytokines, such as CRP, IL-1, TNFα. To additionally evaluate causal relationships 41 cytokines IBD, a Mendelian randomization (MR) study was conducted. Methods two-sample MR investigation utilized data from three large publicly available genome-wide association studies (GWAS) on ulcerative colitis (UC), Crohn's (CD) genetic variants. Additionally, cytokine GWAS meta-analysis, including 8,293 healthy individuals, were incorporated into study. Causal exposures outcomes predominantly determined utilizing inverse variance-weighted methods. heterogeneity, pleiotropy, stability of these variants, MR-Egger intercept test, Cochran's Q leave-one-out sensitivity analysis Results findings revealed that IL13 linked to an elevated risk UC, CD, while MIF demonstrated correlation with CD. Conversely, TNF-related apoptosis-inducing ligand (TRAIL) decreased IBD UC. reverse analyses correlated levels Monokine Induced by Gamma Interferon (MIG) Stromal Cell-Derived Factor-1α (SDF1A), UC showed MIG IL10. CD stem cell factor (SCF) TNF-β. Conclusion In study, upstream regulatory factors five downstream identified for its subtypes, providing avenues developing new therapies IBD.

Language: Английский

Platelet-Derived Microparticles and Autoimmune Diseases DOI Open Access

Xiaoshuai Li,

Qiushi Wang

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 10275 - 10275

Published: June 17, 2023

Extracellular microparticles provide a means of cell-to-cell communication and can promote information exchanges between adjacent or distant cells. Platelets are cell fragments that derived from megakaryocytes. Their main functions to stop bleeding, regulate inflammation, maintain the integrity blood vessels. When platelets activated, they perform related tasks by secreting platelet-derived contain lipids, proteins, nucleic acids, even organelles. There differences in circulating platelet levels many autoimmune diseases, including rheumatoid arthritis, systemic lupus erythematosus, antiphospholipid antibody syndrome, Sjogren’s syndrome. In this paper, latest findings research field reviewed, potential pathogenesis various types immune their as markers, for monitoring progress prognosis disease treatment expounded.

Language: Английский

Citations

15
Phlorofucofuroeckol-A refined by edible brown algae Ecklonia cava indicates anti-inflammatory effects on TNF-α/IFN-γ-stimulated HaCaT keratinocytes and 12-O-tetradecanoylphorbol 13-acetate-induced ear edema in BALB/c mice DOI Creative Commons
Kirinde Gedara Isuru Sandanuwan Kirindage, Arachchige Maheshika Kumari Jayasinghe, Eui-Jeong Han

et al.

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 109, P. 105786 - 105786

Published: Sept. 10, 2023

The present study investigated the anti-inflammatory potential of phlorofucofuroeckol-A (PFF-A) from Ecklonia cava in TNF-α/IFN-γ-stimulated HaCaT keratinocytes and an acute ear edema mice model. Upon investigation, PFF-A had no cytotoxicity up to 62.5 μg/mL indicated a dose-dependent-cytoprotective effect with reduction intracellular ROS keratinocytes. downregulated mRNA expression inflammatory cytokines (IL-25, IL-33, TSLP, IL-1β, IL-6, IL-8, IL-13, IFN-γ, TNF-α) chemokines (Eotaxin, MDC, RANTES, TARC) Furthermore, inhibited activation NFκB/MAPK signaling whereas activated Nrf2/HO-1 signaling. As extended evaluation, treatment significantly decreased thickness mediators including iNOS COX-2 TPA-induced This suggests skin E. consisting develop functional foods that have effects on abnormal immune responses.

Language: Английский

Citations

14
Platelets and inter-cellular communication in immune responses: Dialogue with both professional and non-professional immune cells DOI
Puja Kumari, Abhishek Panigrahi, Pooja Yadav

et al.

Advances in protein chemistry and structural biology, Journal Year: 2024, Volume and Issue: unknown, P. 347 - 379

Published: Jan. 1, 2024

Language: Английский

Citations

2
Severe Allergy as a Chronic Inflammatory Condition From a Systems Biology Perspective DOI Creative Commons
María Isabel Delgado Dolset, Carmela Pablo‐Torres, N. Contreras

et al.

Clinical & Experimental Allergy, Journal Year: 2024, Volume and Issue: 54(8), P. 550 - 584

Published: June 27, 2024

Persistent and unresolved inflammation is a common underlying factor observed in several seemingly unrelated human diseases, including cardiovascular neurodegenerative diseases. Particularly, atopic conditions, acute inflammatory responses such as those triggered by insect venom, food or drug allergies possess also life-threatening potential. However, respiratory predominantly exhibit late immune associated with chronic inflammation, that can eventually progress into severe phenotype displaying similar features other the case of uncontrolled asthma. This review aims to explore different facets systems involved allergic processes tissue remodelling cell dysregulation, well genetic, metabolic microbiota alterations, which are conditions. Our goal here was deepen on understanding an entangled disease expose potential avenues for development better diagnostic intervention strategies.

Language: Английский

Citations

1
CD40‐CD40 ligand interaction between periodontal ligament cells and cementoblasts enhances periodontal tissue remodeling in response to mechanical stress DOI
Yu Yamamoto, Chiharu Fujihara,

Teerachate Nantakeeratipat

et al.

Journal of Periodontal Research, Journal Year: 2023, Volume and Issue: 58(6), P. 1261 - 1271

Published: Sept. 18, 2023

Abstract Objective We analyzed the localization and expression of Cluster differentiation 40 ligand (CD40L) in murine periodontal tissue applied with orthodontic force to determine CD40L‐expressing cells under mechanical stress. Furthermore, we investigated whether CD40‐CD40L interaction played an important role transducing stress between ligament (PDL) cementoblasts remodeling for its homeostasis. Background PDL is a complex that contains heterogeneous cell populations constantly exposed stress, such as occlusal force. CD40 expressed on upregulated However, ligand, CD40L, response which functions induces by converting biological cement‐PDL complex, are not fully understood. Methods The treatment was first molars at left side upper maxillae mice using nickel‐titanium closed‐coil spring. Immunohistochemistry performed analyze CD40L Human (HCEM) human were stretched vitro protein flow cytometry. A GFP‐expressing plasmid vector transfected into HCEM (CD40L‐HCEM). CD40L‐HCEM co‐cultured higher alkaline phosphatase (ALP) activity (hPDS) or lower ALP (hPDF). After co‐culturing, viability proliferation propidium iodide (PI) staining bromodeoxyuridine (BrdU) assay. mRNA cytodifferentiation‐ extracellular matrix (ECM)‐related genes real‐time PCR. Results demonstrated induced present cementum surface tension mice. cytometry showed vitro‐stretching cells. Co‐culturing hPDF enhanced but did alter gene related cytodifferentiation ECM. In contrast, co‐culturing hPDS ECM‐related affect proliferation. Conclusion revealed stretching force, cementoblasts. may interact necessary time location, inducing viability, proliferation, cytodifferentiation, maintaining

Language: Английский

Citations

3
Human B-cells can form hetero-aggregates with blood platelets: a novel insight into adaptive immunity regulation in multiple sclerosis DOI Creative Commons
Karina Maciak, Angela Dziedzic, Jacek Szymański

et al.

Journal of Molecular Biology, Journal Year: 2024, Volume and Issue: unknown, P. 168885 - 168885

Published: Nov. 1, 2024

Multiple sclerosis (MS) is a chronic autoimmune disease characterized by inflammation and neurodegeneration. Our original study analyzes the interactions between blood platelets leukocytes in MS, focused on their potential role modulating immune responses. We demonstrated, for first time, significant increase leukocyte migration towards platelets, indicating higher chemotactic capabilities MS. This novel finding supported microscopic imaging of platelet-leukocyte hetero-aggregates (PLAs). included platelet activation status platelet-lymphocyte cross-talk analysis distinguishing lymphocytic subpopulation patients with relapsing-remitting (RRMS) secondary progressive MS (SPMS) compared to healthy controls (HC). Flow cytometry method revealed an elevated expression typical markers i.e. PAC-1 CD62P both phenotypes especially RRMS, GPVI level SPMS. Detailed immunophenotyping confocal showed increased pool aggregates (PLAs-Ly), particularly involving B-cells over T-cells across phenotypes. The also explored involvement CD40-CD40L pathway, discovering correlations CD40L antigen CD40, may indicate special significance platelet-B-cell phenotype. research identified interaction pathways that influence lymphocyte-mediated response highlighting unexplored formation platelet-B cell (PLAs-LyB).

Language: Английский

Citations

0
Mendelian randomization analyses of genetically predicted circulating levels of cytokines with risk of Inflammatory bowel disease DOI Creative Commons
Xianqiang Liu, Dingchang Li, Wenxing Gao

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 12, 2023

Abstract Objective The literature has previously reported the associations between inflammatory bowel disease (IBD) and certain cytokines, such as CRP, IL-1, TNFα. To additionally evaluate causal relationships 41 cytokines IBD, a Mendelian randomization (MR) study was conducted. Methods two-sample MR investigation utilized data from three large publicly available genome-wide association studies (GWAS) on ulcerative colitis (UC), Crohn's (CD) genetic variants. Additionally, cytokine GWAS meta-analysis, including 8,293 healthy individuals, were incorporated into study. Causal exposures outcomes predominantly determined utilizing inverse variance-weighted methods. heterogeneity, pleiotropy, stability of these variants, MR-Egger intercept test, Cochran's Q leave-one-out sensitivity analysis Results findings revealed that IL13 linked to an elevated risk UC, CD, while MIF demonstrated correlation with CD. Conversely, TNF-related apoptosis-inducing ligand (TRAIL) decreased IBD UC. reverse analyses correlated levels Monokine Induced by Gamma Interferon (MIG) Stromal Cell-Derived Factor-1α (SDF1A), UC showed MIG IL10. CD stem cell factor (SCF) TNF-β. Conclusion In study, upstream regulatory factors five downstream identified for its subtypes, providing avenues developing new therapies IBD.

Language: Английский

Citations

0