Hypoxic colorectal cancer‐derived extracellular vesicles deliver microRNA‐361‐3p to facilitate cell proliferation by targeting TRAF3 via the noncanonical NF‐κB pathways

Clinical and Translational Medicine, Journal Year: 2021, Volume and Issue: 11(3)

Published: March 1, 2021

Abstract Background Hypoxic tumour microenvironment (TME) is a key regulator in cancer progression. However, the communications between hypoxic cells and other components TME during colorectal (CRC) progression via extracellular vesicles (EVs) remain unclear. Methods High‐throughput sequencing was employed to detect aberrantly expressed microRNAs (miRNAs) EVs. Quantitative real‐time PCR used confirm screen preliminarily candidate miRNAs. The effects of EVs derived from hypoxia (<1% O 2 ) miR‐361‐3p on CRC growth were assessed using CCK‐8 assays, colony formation EdU flow cytometric assays mouse xenograft. Then, specific mechanisms investigated by RNA immunoprecipitation, luciferase reporter assay, Western blot, chromatin immunohistochemistry rescue experiments. Results level expression remarkably elevated can be transferred cells. Functional experiments exhibited that facilitated cell suppressed apoptosis transferring CRC. Hypoxia‐inducible factor‐1α induced elevation levels Upregulated inhibited directly targeting TNF receptor‐associated factor 3, which consequently activated noncanonical NF‐κB pathway. Moreover, high circulating exosomal correlated worse prognosis patients. Conclusions Altogether, abnormality acts vital roles regulation an emerging prognostic biomarker therapeutic target for

Language: Английский

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LncRNAs and CircRNAs in cancer

MedComm, Journal Year: 2022, Volume and Issue: 3(2)

Published: May 12, 2022

Abstract It is well known that noncoding RNAs (ncRNAs) cannot encode proteins, but they can play important regulatory roles in tumors by combining with RNAs, and DNAs. As more studies reveal the underlying mechanisms of long (lncRNAs) circular (circRNAs) cancer, their huge application potential cancer therapy be ignored. For example, lncRNAs involved tumor‐related signal transduction pathways, cell cycle control, DNA damage, epigenetic regulation, microRNA control. A group confirmed abnormal expression affect progression. Furthermore, as covalently closed continuous ncRNAs, many recent have shown circRNAs effects other biological significances cancer. Interestingly, were found to translational functions. This has greatly attracted people's attention research. In this review, we introduce some representative cancers, respectively. focus on functions clinical therapeutic implications lnRNAs neuroblastoma. Our review also focuses providing rationale relevant references for novel biomarkers neuroblastoma diagnosis, prognosis, treatment.

Language: Английский

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Role of non-coding RNAs and exosomal non-coding RNAs in retinoblastoma progression

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Dec. 23, 2022

Retinoblastoma (RB) is a rare aggressive intraocular malignancy of childhood that has the potential to affect vision, and can even be fatal in some children. While tumor controlled efficiently at early stages, metastatic tumors lead high mortality. Non-coding RNAs (ncRNAs) are implicated number physiological cellular process, including differentiation, proliferation, migration, invasion, The deregulation ncRNAs correlated with several diseases, particularly cancer. categorized into two main groups based on their length, i.e. short long ncRNAs. Moreover, ncRNA been demonstrated play role pathogenesis development RB. Several ncRNAs, such as miR-491-3p, miR-613,and SUSD2 have found act suppressor genes RB, but other circ-E2F3, NEAT1, TUG1 promoter genes. Understanding regulatory mechanisms provide new opportunities for RB therapy. In present review, we discuss functional roles most important interaction responsible initiation progression, possible future clinical applications diagnostic prognostic tools or therapeutic targets.

Language: Английский

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Comprehensive insights into circular RNAs, miRNAs, and lncRNAs as biomarkers in retinoblastoma

Ophthalmic Genetics, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: Jan. 23, 2025

Retinoblastoma (RB) is a common and potentially lethal cancer that primarily affects young children worldwide, with survival rates significantly varying between high- low-income countries. This review aims to identify essential diagnostic markers for early diagnosis by investigating the molecular pathways associated RB. The prevalence of RB cases notably concentrated in Asia Africa, contributing global rate estimate less than 30%. Current management strategies involve complex, individualized treatment plans consider cultural nuances, genetic abnormalities, staging, availability medical resources. Recent studies suggest circular RNAs (circRNAs) may serve as predictive biomarkers etiology examines roles long non-coding (lncRNAs), microRNAs (miRNAs), circRNAs RB, goal improving rates, particularly low- middle-income A deeper understanding facilitate development personalized targeted therapies. Elevated expression has been observed most patient cases, indicate reducing specific circRNA production can inhibit tumor cell progression. Investigating mechanisms holds promise future approaches.

Language: Английский

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Publication Trends of Research on Retinoblastoma During 2001–2021: A 20-Year Bibliometric Analysis

Frontiers in Medicine, Journal Year: 2021, Volume and Issue: 8

Published: May 21, 2021

Background: Retinoblastoma is the most common primary intraocular malignancy of childhood. Despite high survival and eye salvage as result various types therapies, retinoblastoma remains a disease that places considerable burden on developing countries. Our study attempted to analyse research trends in compare contributions from different countries, institutions, journals, authors. Methods: We extracted all publications concerning 2001 2021 Web Science database. Microsoft Excel VOSviewer were employed collect publication data, trends, visualize relevant results. Results: A total 1,675 with 30,148 citations identified. The United States contributed (643) (16,931 times) highest H-index value (67) February 4, 2021. China ranked second number (259), while ranking fourth both (2,632 (26) fourth. British Journal Ophthalmology was productive journal retinoblastoma, Abramson DH had published papers field. Keywords categorized into three clusters; tumor-related research, clinical management-related research. keywords “intravitreal,” “intraarterial,” “intravenous” appeared frequently, average appearing year being 2018.1, 2017.7, 2017.1, respectively. Management-related has been recognized heavily researched topic Conclusion: conclude States, China, India made exceptional field still disparity between quantity quality publications. including intravitreal, intraarterial, intravenous chemotherapy considered potential focus for future

Language: Английский

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ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin‐3 and NRXN3

Cancer Communications, Journal Year: 2020, Volume and Issue: 40(12), P. 721 - 737

Published: Oct. 10, 2020

Abstract Background Epigenetic regulation plays an important role in the development and progression of nasopharyngeal carcinoma (NPC). However, epigenetic mechanisms underlying NPC metastasis remains poorly understood. We aimed to find functional genes which regulate identify therapeutic targets for treatment. Methods Bisulfite pyrosequencing was used analyze zinc finger protein 582 ( ZNF582 ) methylation tissues cell lines. Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) Western blotting were determine expression ZNF582. In vitro vivo experiments performed evaluate biological function NPC. ZNF582‐targeting identified by chromatin immunoprecipitation sequencing (ChIP‐seq) confirmed ChIP‐qPCR luciferase assay. Results promoter hypermethylated NPC, both mRNA levels down‐regulated The restoration inhibited migration, invasion, metastasis, while knockdown promoted . directly regulated transcription adhesion molecules Nectin‐3 NRXN3 Both as ZNF582, or abrogation these reversed tumor suppressor effect metastasis. Conclusions acts a gene regulating , may provide novel

Language: Английский

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Osthole exhibits an antitumor effect in retinoblastoma through inhibiting the PI3K/AKT/mTOR pathway via regulating the hsa_circ_0007534/miR-214-3p axis

Pharmaceutical Biology, Journal Year: 2022, Volume and Issue: 60(1), P. 417 - 426

Published: Feb. 17, 2022

Osthole shows antitumor effects in various tumours. Studies describing the effect of osthole on retinoblastoma (RB) are rare.This study investigates activity RB.RB cells were treated with different concentrations and then subjected to cell viability, colony formation, apoptosis, western blot assays. The expression hsa_circ_0007534 RB tissues was determined by qRT-PCR. Hsa_circ_0007534 overexpression plasmid (oe-circ_0007534), miR-214-3p mimics negative controls transfected into investigate viability. Athymic nude mice injected Y-79 establish subcutaneous models. These (0.5 mmol/kg) or corn oil for 36 days. Tumour collected further analysis.Osthole inhibited viability an IC50 200 μM 24 h treatment 120 48 treatment, respectively. increased significantly as compared matched nontumor (p < 0.001). Oe-circ_0007534 counteracted inhibitory numbers 0.01). In vivo experiments indicated decreased 0.01) level vivo. Furthermore, control, ratios p-PI3K/PI3K, p-AKT/AKT p-mTOR/mTOR However, reversed PI3K/AKT/mTOR pathway.Osthole exhibited antitumour RB, providing a scientific basis research clinical applications treatment.

Language: Английский

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MiR-361-3p alleviates cerebral ischemia–reperfusion injury by targeting NACC1 through the PINK1/Parkin pathway

Journal of Molecular Histology, Journal Year: 2022, Volume and Issue: 53(2), P. 357 - 367

Published: Jan. 24, 2022

Language: Английский

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CircMKLN1 Suppresses the Progression of Human Retinoblastoma by Modulation of miR-425-5p/PDCD4 Axis

Current Eye Research, Journal Year: 2021, Volume and Issue: 46(11), P. 1751 - 1761

Published: May 14, 2021

Purpose: Circular RNAs (circRNAs) are essential regulators in tumorigenesis and development. In this study, we focused on the functions of circRNA muskelin 1 (circMKLN1) retinoblastoma (RB) progression.Materials Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was conducted to determine levels circMKLN1, microRNA-425-5p (miR-425-5p) programmed cell death 4 (PDCD4). The characteristic circMKLN1 analyzed using RNase R assay. Cell Counting Kit-8 (CCK-8) colony formation were employed explore proliferation ability. transwell utilized for migration invasion. A Western blot performed protein levels. dual-luciferase reporter RNA immunoprecipitation (RIP) demonstrate relationships among miR-425-5p PDCD4. Murine xenograft model adopted investigate role vivo.Results: CircMKLN1 downregulated RB tissues cells. High related a favorable outcome patients. resistant digestion overexpression repressed proliferation, invasion vitro. MiR-425-5p identified as target elevation reversed effects malignant behaviors. Furthermore, gene miR-425-5p, PDCD4 silencing could ameliorate suppressive roles growth metastasis. Additionally, hampered tumor vivo.Conclusions: decelerated progression through sponging elevating

Language: Английский

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Circ_0000527 Promotes Retinoblastoma Progression through Modulating miR-98-5p/XIAP Pathway

Current Eye Research, Journal Year: 2021, Volume and Issue: 46(9), P. 1414 - 1423

Published: Feb. 25, 2021

Purpose: Retinoblastoma (RB) is an intraocular malignancy that often occurs in childhood. Circular RNAs (circRNAs) play crucial roles regulating the malignant phenotypes of various tumors. This study aimed to explore role and potential mechanism circ_0000527 RB.Methods: The levels circ_0000527, microRNA-98-5p (miR-98-5p) X-linked inhibitor apoptosis (XIAP) were determined by qRT-PCR or western blot. Cell proliferation was detected 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) colony formation assays. apoptosis, migration invasion evaluated flow cytometry, transwell scratch Xenograft assay conducted analyze tumor growth vivo. binding relationship between miR-98-5p XIAP verified dual-luciferase reporter RNA immunoprecipitation (RIP) assays.Results: Circ_0000527 increased, while level reduced RB tissues cells. Silencing suppressed proliferation, cells promoted apoptosis. In addition, knockdown inhibited xenograft mice. Besides, sponged regulate cell progression, targeted mediate development. Moreover, modulated expression via sequestering miR-98-5p.Conclusion: facilitated progression miR-98-5p/XIAP axis, which provided a promising therapeutic target for RB.

Language: Английский

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