Unveiling the Thermotolerance and Growth-Promoting Attributes of Endophytic Bacteria Derived from Oryza sativa: Implications for Sustainable Agriculture DOI Creative Commons
Wonder Nathi Dlamini, Wei-An Lai, Wen‐Ching Chen

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 766 - 766

Published: March 27, 2025

High temperatures pose significant challenges to rice plants’ growth and their associated endophytic bacteria. Understanding how these bacteria respond heat stress is vital. We assessed the potential of five bacterial strains derived from Oryza sativa—Bacillus tequilensis LB3, B. coagulans LB6, paralicheniformis AS9, pumilus LB16, paranthracis i40C—to mitigate effects on plants. These demonstrated robust abilities in producing indole-3-acetic acid (IAA) siderophores, nitrogen fixation, solubilization phosphate potassium. Under high-temperature conditions, they significantly enhanced plant growth, with increases length up 78% at 40 °C. Notably, LB6 showed highest biomass increase (195%). The also improved chlorophyll SPAD values, an indicator reduced health. Phytohormone profiling biochemical analyses revealed abscisic (ABA) levels, lipid peroxidation (MDA), elevated osmoprotectant proline accumulation under stress. Inoculated plants exhibited 539 ng g−1 ABA (vs. 62 uninoculated controls), a 68% reduction MDA (indicating less oxidative damage), synthesis, collectively suggesting adaptation. changes were linked IAA production nutrient modulation, which alleviated heat-induced physiological decline. findings underscore endophytes as biofertilizers improve resilience Among strains, superior performance, offering greatest promise for heat-stress mitigation production. This study advances our understanding phytohormonal, signaling, chemical processes underlying bacterial-mediated thermotolerance, providing foundation sustainable agricultural strategies. Future research can explore morphological analyses, stress-responsive gene expression (e.g., HSPs, DREBs, APX) combined selected fertilizers cultivation.

Language: Английский

Microorganisms in Plant Growth and Development: Roles in Abiotic Stress Tolerance and Secondary Metabolites Secretion DOI Creative Commons
Ntombikhona Koza, Afeez Adesina Adedayo, Olubukola Oluranti Babalola

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(8), P. 1528 - 1528

Published: July 28, 2022

Crops aimed at feeding an exponentially growing population are often exposed to a variety of harsh environmental factors. Although plants have evolved ways adjusting their metabolism and some also been engineered tolerate stressful environments, there is still shortage food supply. An alternative approach explore the possibility using rhizosphere microorganisms in mitigation abiotic stress hopefully improve production. Several studies shown that rhizobacteria mycorrhizae organisms can help tolerance by enhancing plant growth; stimulating production phytohormones, siderophores, solubilizing phosphates; lowering ethylene levels; upregulating expression dehydration response antioxidant genes. This article shows secretion secondary metabolites as additional mechanism employed against stress. The understanding these mechanisms will efficacy plant-growth-promoting microorganisms.

Language: Английский

Citations

177
Plant probiotics – Endophytes pivotal to plant health DOI Creative Commons
Shiv Shanker Pandey, Rahul Jain, Priyanka Bhardwaj

et al.

Microbiological Research, Journal Year: 2022, Volume and Issue: 263, P. 127148 - 127148

Published: July 27, 2022

Language: Английский

Citations

69
Endophytic bacteria for Cd remediation in rice: Unraveling the Cd tolerance mechanisms of Cupriavidus metallidurans CML2 DOI Creative Commons
Yuan Zhang,

Qi Zhou,

Chang Gao

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133846 - 133846

Published: Feb. 23, 2024

The utility of endophytic bacteria in Cadmium (Cd) remediation has gained significant attention due to their ability alleviate metal-induced stress and enhance plant growth. Here, we investigate C. metallidurans CML2, an bacterial strain prevalent rice, showing resilience against 2400 mg/L Cd(II). We conducted in-depth integrated morphological transcriptomic analysis illustrating the multifarious mechanisms CML2 employs combat Cd, including formation biofilm CdO nanoparticles, upregulation genes involved periplasmic immobilization, utilization RND efflux pumps extract excess Cd ions. Beyond exhibited robust tolerance array heavy metals, Cd2+, Mn2+, Se4+, Ni2+, Cu2+, Hg2+, demonstrating effective Cd(II) removal capacity. Furthermore, growth-promoting properties through production indole-3-acetic acid (IAA) at 0.93 mg/L, soluble phosphorus compounds 1.11 siderophores 22.67%. Supportively, pot experiments indicated increase root lengths a decrease bioaccumulation rice seedlings inoculated with consequently reducing translocation rates from 43% 31%. These findings not only contribute understanding resistance but also underscore CML2's promising application within farming ecosystems. Rice Cupriavidus tolerate high cadmium levels (2400 mg/L) its inherent defense make it potential candidate for bio-remediation agriculture. When applied cultivation, reduces soil toxicity crops promotes Consequently, contributes safer food supply, bolsters ecological sustainability, offers viable alternative chemical practices, public health.

Language: Английский

Citations

15
Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions DOI Creative Commons

Faisal Mehdi,

Zhengying Cao,

Shuzhen Zhang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 13, 2024

Environmental stresses are the main constraints on agricultural productivity and food security worldwide. This issue is worsened by abrupt severe changes in global climate. The formation of sugarcane yield accumulation sucrose significantly influenced biotic abiotic stresses. Understanding biochemical, physiological, environmental phenomena associated with these essential to increase crop production. review explores effect factors content highlights negative effects insufficient water supply, temperature fluctuations, insect pests, diseases. article also explains mechanism reactive oxygen species (ROS), role different metabolites under stresses, function stress-related resistance genes sugarcane. further discusses improvement approaches, a focus endophytic consortium endophyte application plants. Endophytes vital plant defense; they produce bioactive molecules that act as biocontrol agents enhance immune systems modify responses through interaction provides an overview internal mechanisms growth offers new ideas for improving fitness productivity.

Language: Английский

Citations

13
Identification of in planta bioprotectants against Fusarium wilt in Medicago sativa L. (lucerne) from a collection of bacterial isolates derived from Medicago seeds DOI Creative Commons

Shenali Subodha Herath Dissanayakalage,

Jatinder Kaur, Saidi R. Achari

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Fusarium wilt caused by oxysporum f. sp. medicaginis (Fom) is an important disease affecting lucerne/alfalfa cultivations worldwide. Medicago sativa L. (lucerne) one of the major legume crops in global forage industry. This study aimed to identify bacteria capable biologically controlling pathogen through a comprehensive screening bacterial isolates obtained from domesticated and wild growing seeds. Using multi-tiered evaluation pipeline, including vitro, soil-free potting mix-based pathogenicity bioprotection assay systems, efficacy 34 derived seeds was initially evaluated against six strains vitro. (Fo) F5189, which has previously been characterized as isolate causing lucerne selected for planta assays. Lucerne cultivars Grazer Sequel, representing susceptible resistant genotypes were chosen assess progression. Pathogenicity time-course studies conducted understand temporal dynamics host-pathogen interactions bioprotectants. The symptoms scored using rating index developed this study. results indicated variability across isolates, with some suppressing both systems. Paenibacillus (Lu_MgY_007; NCBI: PQ756884) Pseudomonas (Lu_LA164_018; PQ756887) identified promising bioprotectants under tested growth conditions. highlighted critical role persistent biocontrol activity precise timing application achieving long-term suppression. Overall, observed reduction severity underscores potential these sustainable strategies managing cultivars. However, molecular-level analyses are warranted elucidate underlying mechanisms, offering valuable insights development more effective future agricultural

Language: Английский

Citations

1
The rice endophytic bacterium Bacillus velezensis LS123N provides protection against multiple pathogens and enhances rice resistance to wind with increase in yield DOI Creative Commons

Chi-Kuan Tu,

Wen-Di Huang,

Pei‐Han Wang

et al.

Biological Control, Journal Year: 2024, Volume and Issue: 192, P. 105507 - 105507

Published: March 25, 2024

Rice (Oryza sativa) is a globally important staple food, but its production was influenced by various biotic and abiotic stresses. This study involved screening endophytic bacterial strains for their antagonistic activities control efficacy against multiple rice pathogens in planta assays. Subsequent greenhouse investigations identified Bacillus velezensis LS123N as the most promising strain effectively controlling diseases, including blight, blast, bakanae disease, Pythium brown spot disease. Field trials further demonstrated that significantly reduced naturally occurring disease caused Bipolaris oryzae both seedlings mature plants, increased yields. For spot, only single treatment during seed germination resulted significant efficacy. The application of aligns with common cultivation practices. Additionally, exhibited capacity to improve resistance wind stress enhance growth seedling stage. Endophytic colonization evidenced from plants after treatment. Interestingly, expression many defense genes suppressed within 48 h introduction. In vitro assays showed inhibited spore normal germ tubes B. oryzae, produced hydrolases, siderophores, IAA, had ability solubilize phosphorus compounds. summary, these results indicate biocontrol agent has potential be developed into biofungicide, biofertilizer, biostimulator.

Language: Английский

Citations

8
Bacterial Endophytes and Their Contributions to Alleviating Drought and Salinity Stresses in Wheat: A Systematic Review of Physiological Mechanisms DOI Creative Commons

Fayha M. Al-Hawamdeh,

Jamal Y. Ayad, Kholoud M. Alananbeh

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(5), P. 769 - 769

Published: May 16, 2024

Drought and salinity stresses significantly threaten global wheat productivity, limiting growth reducing yields, thus endangering food security worldwide. These disrupt physiological processes, impair photosynthesis, hinder optimal yield by diminishing water uptake, causing osmotic stress, ion toxicity, oxidative stress. In response, various mitigation strategies have been explored, including breeding for stress-tolerant cultivars, improved irrigation techniques, the application of exogenous osmoprotectants soil amendments. Among these strategies, emergence rhizospheric endophytic growth-promoting microorganisms has attracted significant attention. Therefore, a systematic review was undertaken to illustrate role bacteria in enhancing tolerance drought stresses. This analyzes mechanisms research trends, identifies gaps, discusses implications sustainable agriculture. An analysis literature related conducted using databases major publishers from 2004 2023. The explores their mechanisms, such as phytohormone production stress-responsive gene induction, emphasizing contribution plant stress resilience. current trends indicate growing interest utilizing mitigate cultivation, with studies focusing on understanding responses interactions plants. Future should concentrate elucidating host multiple stressors, well aspects like mechanism action, lifestyle, transmission pathways. Overall, offer promising avenues agricultural practices, aiding crop resilience amid environmental challenges.

Language: Английский

Citations

6
Plant Abiotic and Biotic Stress Alleviation: From an Endophytic Microbial Perspective DOI
Vivek Kumar, Chandra Shekhar Nautiyal

Current Microbiology, Journal Year: 2022, Volume and Issue: 79(10)

Published: Sept. 10, 2022

Language: Английский

Citations

28
Transcriptome Landscapes of Salt-Susceptible Rice Cultivar IR29 Associated with a Plant Growth Promoting Endophytic Streptomyces DOI Creative Commons
Worarat Kruasuwan, Karan Lohmaneeratana, John Munnoch

et al.

Rice, Journal Year: 2023, Volume and Issue: 16(1)

Published: Feb. 4, 2023

Plant growth-promoting endophytic (PGPE) actinomycetes have been known to enhance plant growth and mitigate from abiotic stresses via their PGP-traits. In this study, PGPE Streptomyces sp. GKU 895 promoted alleviated salt tolerance of salt-susceptible rice cultivar IR29 by augmentation weight declined ROS after irrigation with 150 mM NaCl in a pot experiment. Transcriptome analysis exposed the combination strain salinity demonstrated up downregulated differentially expressed genes (DEGs) classified gene ontology reactome. induced changes expression including transcription factors under treatment which involved development, photosynthesis, hormones, scavenging, ion transport homeostasis, plant-microbe interactions regarding pathogenesis- symbiosis-related proteins. Taken together, these data demonstrate that colonized enhanced triggered phenotype. Our findings suggest utilisation beneficial endophytes saline fields could allow for use such marginal soils growing possibly other crops.

Language: Английский

Citations

16
Screening of endophytic Bacteria from stress-tolerating plants for abiotic stress tolerance and plant growth-promoting properties: Identification of potential strains for bioremediation and crop enhancement DOI Creative Commons
Manpreet Kaur, Arun Karnwal

Journal of Agriculture and Food Research, Journal Year: 2023, Volume and Issue: 14, P. 100723 - 100723

Published: July 28, 2023

Enhancing plant productivity and fortifying defense mechanisms against biotic abiotic stresses is crucial for sustainable agriculture. To achieve this, researchers have investigated the use of endophytic bacteria as a viable alternative to chemical fertilizers pesticides. In this study, 50 microbes were isolated from stress-tolerant parts different plants, including Lantana camara, Phoenix dactylifera, Hemerocallis fulva, Salvia rosmarinus, Commiphora wightii, Abutilon indicum. These evaluated their tolerance ability promote growth. Among isolates, R1L2 A2L2L2 exhibited highest salt stress, withstanding up 16% NaCl. DL2R2, R1L2, A1S1S most tolerant lead tolerating 6% w/v lead. For cadmium DL3R2, DP1L1L1 showed tolerance, enduring cadmium. The isolates also assessed growth-promoting (PGP) activities, production IAA, Gibberellin, Cytokinin, HCN, siderophore, phosphate solubilization, nitrogen fixation. All positive fixation attribute. However, under specific stress conditions, all negative results HCN production, siderophore or inorganic solubilization. Isolates DL3R2 demonstrated producing phytohormones such Cytokinin. Through phenotypic biochemical characteristics, well 16S rDNA gene sequencing, isolate was identified Pelomonas aquatic (accession no. OP984335), Solibacillus silvestris OP854919). findings suggest that strains potential applications multi-stress reducers, bioremediation agents, crop growth promoters in essential crops.

Language: Английский

Citations

14