Genome engineering for crop improvement and future agriculture

Cell, Journal Year: 2021, Volume and Issue: 184(6), P. 1621 - 1635

Published: Feb. 15, 2021

Language: Английский

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Discovery of deaminase functions by structure-based protein clustering

Cell, Journal Year: 2023, Volume and Issue: 186(15), P. 3182 - 3195.e14

Published: June 27, 2023

Language: Английский

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Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 18, 2023

Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery engineered nucleases as genetic scissors carry out precise manipulation in crop stress-responsive genes associated molecular network has paved way for sustainable management abiotic stress conditions. In this context, clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool revolutionized due its simplicity, accessibility, adaptability, flexibility, wide applicability. This system great potential build up varieties with enhanced tolerance against review, we summarize latest findings on understanding mechanism response plants application CRISPR/Cas-mediated towards a multitude stresses metals. We provide mechanistic insights CRISPR/Cas9-based genome editing technology. also discuss applications evolving techniques such prime base editing, mutant library production, transgene free multiplexing rapidly deliver modern cultivars adapted

Language: Английский

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Enhancing the productivity and resilience of rice (Oryza sativa) under environmental stress conditions using clustered regularly interspaced short palindromic repeats (CRISPR) technology

Functional Plant Biology, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 2, 2025

Rice (Oryza sativa ) is a crucial staple crop worldwide, providing nutrition to more than half of the global population. Nonetheless, sustainability grain production increasingly jeopardized by both biotic and abiotic stressors exacerbated climate change, which increases crop's rvulnerability pests diseases. Genome-editing clustered regularly interspaced short palindromic repeats CRISPR-associated Protein 9 (CRISPR-Cas9) presents potential solution for enhancing rice productivity resilience under climatic stress. This technology can alter plant's genetic components without introduction foreign DNA or genes. It has become one most extensively used approaches discovering new gene functions creating novel varieties that exhibit higher tolerance stresses, herbicide resistance, improved yield production. study examines numerous CRISPR-Cas9-based genome-editing techniques knockout, knock-in, multiplexing simultaneous disruption multiple genes, base-editing, prime-editing. review elucidates application technologies enhance directly targeting yield-related genes indirectly modulating stress-responsive We highlight need integrate advancements with conventional advanced agricultural methods create are resilient thereby safeguarding food security promoting amid concerns.

Language: Английский

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The Development of Herbicide Resistance Crop Plants Using CRISPR/Cas9-Mediated Gene Editing

Genes, Journal Year: 2021, Volume and Issue: 12(6), P. 912 - 912

Published: June 12, 2021

The rapid increase in herbicide-resistant weeds creates a huge challenge to global food security because it can reduce crop production, causing considerable losses. Combined with lack of novel herbicides, cultivating crops becomes an effective strategy control reduced phytotoxicity, and expands the herbicidal spectrum. Recently developed clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas)-mediated genome editing techniques enable efficiently targeted modification hold great potential creating desired plants herbicide resistance. In present review, we briefly summarize mechanism responsible for resistance then discuss applications traditional mutagenesis transgenic breeding crops. We mainly emphasize development use CRISPR/Cas technology improvement. Finally, future system developing

Language: Английский

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Twenty years of rice genomics research: From sequencing and functional genomics to quantitative genomics

Molecular Plant, Journal Year: 2022, Volume and Issue: 15(4), P. 593 - 619

Published: March 21, 2022

Language: Английский

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Enabling technology and core theory of synthetic biology

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 66(8), P. 1742 - 1785

Published: Feb. 6, 2023

Language: Английский

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Breakthrough in CRISPR/Cas system: Current and future directions and challenges

Biotechnology Journal, Journal Year: 2023, Volume and Issue: 18(8)

Published: May 11, 2023

Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists develop desirable varieties. This involves inducing site-specific DNA perturbations that can be repaired through repair pathways. GE products currently include CRISPR-associated nuclease breaks, prime editors generated flaps, single nucleotide-modifications, transposases, recombinases. The discovery of double-strand nucleases (SSNs), mechanisms paved the way for targeted GE, first-generation tools, ZFNs TALENs, were successfully utilized GE. However, CRISPR-Cas now become preferred tool due its speed, reliability, cost-effectiveness. Plant functional genomics benefited significantly from widespread use CRISPR advancements developments. review highlights progress made technology, including multiplex editing, base (BE), (PE), as well challenges potential delivery mechanisms.

Language: Английский

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Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy

Nature Plants, Journal Year: 2023, Volume and Issue: 9(10), P. 1591 - 1597

Published: Sept. 18, 2023

Language: Английский

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Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants

Life, Journal Year: 2023, Volume and Issue: 13(7), P. 1456 - 1456

Published: June 27, 2023

Genome editing aims to revolutionise plant breeding and could assist in safeguarding the global food supply. The inclusion of a 12–40 bp recognition site makes mega nucleases first tools utilized for genome generation gene-editing tools. Zinc finger (ZFNs) are second technique, because they create double-stranded breaks, more dependable effective. ZFNs were original designed nuclease-based approach editing. Cys2-His2 zinc domain’s discovery made this technique possible. Clustered regularly interspaced short palindromic repeats (CRISPR) improve genetics, boost biomass production, increase nutrient usage efficiency, develop disease resistance. Plant genomes can be effectively modified using genome-editing technologies enhance characteristics without introducing foreign DNA into genome. Next-generation will soon defined by these exact methods. There is abroad promise that genome-edited crops essential years come improving sustainability climate-change resilience systems. This method also has great potential enhancing crops’ resistance various abiotic stressors. In review paper, we summarize most recent findings about mechanism stress response crop plants use CRISPR/Cas mediated systems tolerance stresses including drought, salinity, cold, heat, heavy metals.

Language: Английский

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