Multi-omics research in fungus: Current progress and future prospects

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 161 - 211

Published: Jan. 1, 2025

Language: Английский

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The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of Arthrobotrys oligospora

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

The high-osmolarity glycerol (HOG) signalling pathway, comprising Ste11/Ssk2/Ssk22 (MAPKKK), Pbs2 (MAPKK), and Hog1 (MAPK), is an important conserved pathway in fungi. However, the functions downstream regulatory factors of nematode-trapping (NT) fungi remain poorly understood. Here, three proteins (AoNmd5, AoPyp1, AoPtp) interacting with were screened a representative NT fungus Arthrobotrys oligospora using yeast screening library verified two-hybrid (Y2H) assay. function AoNmd5 was furtherly characterized by phenotypic comparison, staining technique, multi-omics analyses. essential for vegetative growth, conidial development, trap morphogenesis, nematode predation ability. In addition, played crucial roles endocytosis, lipid metabolism, reactive oxygen species, stress response, autophagy, other metabolic processes. Furthermore, we constructed interaction network based on transcriptomic analysis Y2H, revealing its significant role respiratory chain redox processes as well small GTPase Ran1, which mediates nucleocytoplasmic shuttling. These findings suggest that Hog1-Nmd5 has pleiotropic A. oligospora. This study deepens our understanding HOG importins

Language: Английский

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The GCN4-Swi6B module mediates low nitrogen-induced cell wall remodeling in Ganoderma lucidum

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

ABSTRACT In natural habitats, microorganisms encounter various unfavorable environmental stresses, including nitrogen deficiency. As the outermost barrier, cell wall plays a crucial role in interaction between and external environment. However, effect of low on thickness, especially underlying molecular mechanism, is unclear. Here, we found that compared with those under normal conditions, both thickness polysaccharide content Ganoderma lucidum are increased conditions. Furthermore, abundance SWI6B, transcription factor participates remodeling, also low-nitrogen environments. The SWI6B -overexpression strains ( SWI6B-OEs ) but decreased SWI6- knockdown swi6-kds ). Moreover, although all genotypes nitrogen-limited percentage upregulated was significantly lower than WT, highest. GCN4, key signaling pathway, to directly bind promoter SWI6 . transcriptional translational levels were reduced GCN4 -knockdown gcn4-kds ), indicating positive regulation by GCN4. Consistently, wild type. Taken together, our results revealed GCN4-Swi6B module regulates remodeling G. deficiency IMPORTANCE To survive stressful environments, fungi initiate pathways adaptively modify composition structure. through GCN4-SWI6B pathway. Our findings provide valuable insights into adaptation fungal walls, contributing deeper understanding responses stress.

Language: Английский

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AMPK Is Involved in Regulating the Utilization of Carbon Sources, Conidiation, Pathogenicity, and Stress Response of the Nematode-Trapping Fungus Arthrobotrys oligospora

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(4)

Published: Aug. 2, 2022

AMP-activated protein kinase (AMPK), a heterotrimeric complex, can sense energy and nutritional status in eukaryotic cells, thereby participating the regulation of multiple cellular processes. In this study, we characterized function catalytic α-subunit (SNF1) two regulatory β- γ-subunits (GAL83 SNF4) AMPK representative nematode-trapping fungus, Arthrobotrys oligospora, by gene knockout, phenotypic analysis, RNA sequencing. The ability complex mutants (including ΔAosnf1, ΔAogal83, ΔAosnf4) to utilize nonfermentable carbon source (glycerol) was reduced, spore yields trap formation were remarkably decreased. Moreover, plays an important role regulating stress response nematode predation efficiency. Transcriptomic profiling between wild-type strain ΔAosnf1 showed that differentially expressed genes enriched for peroxisome, endocytosis, fatty acid degradation, multilipid metabolism (sphingolipid, ether lipid, glycerolipid, glycerophospholipid). Meanwhile, reduced lipid droplet accumulation ΔAosnf4 observed, more vacuoles appeared mycelia mutant. These results highlight utilization sources metabolism, as well providing novel insights into mechanisms development, conidiation, (NT) fungi. IMPORTANCE NT fungi are widely distributed various ecosystems factors control populations nature; their trophic form unique infectious devices (traps) capturing nematodes. oligospora is which develop three-dimensional networks (adhesive networks) predation. Here, demonstrated glycerol utilization, formation, A. further confirmed transcriptomic analysis mutant strains. particular, our indicated required primarily associated with essential formation. Therefore, study extends functional helps elucidate molecular mechanism laying foundation development efficient biocontrol agents.

Language: Английский

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Melanin precursors mediated adaption to temperature changes in fungus and animal via inhibition of lipid-mediated ferroptosis

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 66(8), P. 1800 - 1817

Published: March 17, 2023

Language: Английский

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The APSES transcription factor CfSwi6 is required for growth, cell wall integrity, and pathogenicity of Ceratocystis fimbriata

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127624 - 127624

Published: Jan. 21, 2024

Cell wall integrity (CWI) is crucial for the growth, development, and host invasion of pathogenic fungi. The APSES transcription factor Swi6 in fungi plays a role mediating cell through mitogen-activated protein kinase (MAPK) signaling pathway. Ceratocystis fimbriata notorious fungus responsible causing black rot sweet potatoes. In this study, an orthologous (CfSwi6) downstream CWI regulatory pathway C. was characterized. Deletion CfSWI6 leads to impaired hyphal conidiation, compromised integrity, resulting significant reduction virulence. Transcriptome analysis revealed involvement various pathways, including MAPK pathway, DNA synthesis stress response. ChIP-seq data provided predictions potential target genes regulated by CfSwi6. Through yeast one-hybrid, we confirmed direct binding CfSwi6 promoter chitin synthetase gene. summary, these findings indicated that important pathogenicity fimbriata. This study provides new insights into mechanism potato inspires strategies control rot.

Language: Английский

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The MADS-box transcription factor AoRlmA is involved in the regulation of mycelium development, conidiation, cell-wall integrity, stress response, and trap formation of Arthrobotrys oligospora

Microbiological Research, Journal Year: 2022, Volume and Issue: 268, P. 127299 - 127299

Published: Dec. 29, 2022

Language: Английский

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AoRab7A interacts with AoVps35 and AoVps41 to regulate vacuole assembly, trap formation, conidiation, and functions of proteasomes and ribosomes in Arthrobotrys oligospora

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127573 - 127573

Published: Dec. 12, 2023

Language: Английский

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Cellular communication and fusion regulate cell fusion, trap morphogenesis, conidiation, and secondary metabolism in Arthrobotrys oligospora

Microbiological Research, Journal Year: 2023, Volume and Issue: 278, P. 127516 - 127516

Published: Oct. 12, 2023

Language: Английский

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The Arf-GAP Proteins AoGcs1 and AoGts1 Regulate Mycelial Development, Endocytosis, and Pathogenicity in Arthrobotrys oligospora

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(5), P. 463 - 463

Published: April 29, 2022

Small GTPases from the ADP-ribosylation factor (Arf) family and their activating proteins (Arf-GAPs) regulate mycelial development, endocytosis, virulence in fungi. Here, we identified two orthologous Arf-GAP proteins, AoGcs1 AoGts1, a typical nematode-trapping fungus Arthrobotrys oligospora. The transcription of Aogcs1 Aogts1 was highly expressed sporulation stage. deletion caused defects DNA damage, scavenging reactive oxygen species, lipid droplet storage, mitochondrial activity, autophagy, serine protease response to endoplasmic reticulum stress. combined effects resulted slow growth, decreased capacity, increased susceptibility chemical stressors heat shock, pathogenicity mutants compared with wild-type (WT) strain. Although produced similar phenotfypic traits, roles varied conidiation proteolytic activity. ΔAogts1 mutant showed remarkable reduction conidial yield WT strain but not activity; contrast, ΔAogcs1 an increase activity sporulation. In addition, growth promoted by rapamycin, sensitive H-89. Collectively, more difference than mutant. Our study further illustrates importance Arf-GAPs

Language: Английский

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