ESCRT pathway-dependent MVBs contribute to the morphogenesis of the fungusArthrobotrys oligospora DOI Creative Commons
Mengqing Tian,

Jinrong Huang,

Peijie Cui

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 9, 2022

Abstract Ammonia opens trap formation in the nematode-trapping (NT) fungus Arthrobotrys oligospora , an intriguing morphological switch NT fungi, where saprophytic mycelia are converted to pathogenic organs. Endocytosis plays a prominent role nutrient uptake, signaling cascades, and maintenance of cellular homeostasis higher eukaryotes. Here, we demonstrate that ammonia efficiently promotes endocytosis via 3D-adhesive mycelial nets A. . Trap production is followed by presence massive multivesicular bodies (MVBs) membrane rupture repair. Additionally, both ubiquitin-proteasome system endosomal sorting complex for transport (ESCRT) pathway immediately linked regulation MVB ammonia-induced formation. Moreover, disruption ESCRT-1 subunit proteins AoHse AoVps27 led complete loss Finally, deletion deubiquitinase AoSst2 caused significant reduction number structures produced response exposure or nematodes. Overall, our results increase knowledge molecular mechanisms underlying phenotypic changes fungal group, demonstrating endocytosis-ESCRT-MVB participates trapping Author Summary The lifestyle fungi event increases their pathogenicity nematode prey, which has resulted large losses agricultural crops worldwide. describe mechanism how this group forms structure ammonia, widely preferred nitrogen source soil niches. enhances process, system, model thereby generating enriched during In cell morphology remarkably damaged then repaired. We further found ESCRT-0 subcomplex ubiquitinase severely blocked reorganization. Our study provides new understanding flux transition

Language: Английский

AoSte12 Is Required for Mycelial Development, Conidiation, Trap Morphogenesis, and Secondary Metabolism by Regulating Hyphal Fusion in Nematode-Trapping Fungus Arthrobotrys oligospora DOI Creative Commons
Na Bai,

Meihua Xie,

Qianqian Liu

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: Feb. 14, 2023

Nematode-trapping (NT) fungi are a unique group of carnivorous microorganisms that can capture and digest nematodes by producing ingenious trapping devices (traps).

Language: Английский

Citations

17
Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora DOI Creative Commons
Qianqian Liu, Kexin Jiang,

Shipeng Duan

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing its ability capture by developing specialized traps. A previous showed that high-osmolarity glycerol (Hog1) signaling regulates osmoregulation nematocidal activity of A. oligospora. However, function downstream transcription factors Hog1 in nematode-trapping (NT) remains unclear. This aimed investigate functions potential regulatory network AoMsn2, factor pathway The AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, yeast two-hybrid analysis. Loss Aomsn2 significantly enlarged swollen hyphae, with an increase septa significant decrease nuclei. In particular, spore yield, germination rate, traps, nematode predation efficiency were remarkably decreased mutants. Phenotypic transcriptomic analyses revealed is essential for fatty acid metabolism autophagic pathways. Additionally, metabolomic analysis identified important modulation secondary metabolites. Furtherly, we analyzed protein based on Kyoto Encyclopedia Genes Genomes map online website STRING. Finally, six putative proteins Y2H Our reveals plays crucial roles growth, conidiation, trap development, metabolism, well establishes broad basis understanding mechanisms morphogenesis environmental adaptation NT fungi.

Language: Английский

Citations

6
The cAMP‐PKA signalling pathway regulates hyphal growth, conidiation, trap morphogenesis, stress tolerance, and autophagy in Arthrobotrys oligospora DOI
Meichen Zhu, Na Zhao,

Yan‐Kun Liu

et al.

Environmental Microbiology, Journal Year: 2022, Volume and Issue: 24(12), P. 6524 - 6538

Published: Oct. 19, 2022

The cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signalling pathway is evolutionarily conserved in eukaryotes and plays a crucial role defending against external environmental challenges, which can modulate the cellular response to stimuli. Arthrobotrys oligospora typical nematode-trapping fungus that specializes adhesive networks kill nematodes. To elucidate biological roles of cAMP-PKA pathway, we characterized orthologous adenylate cyclase AoAcy, regulatory subunit (AoPkaR), two catalytic subunits (AoPkaC1 AoPkaC2) PKA A. by gene disruption, transcriptome, metabolome analyses. Deletion Aoacy significantly reduced levels cAMP arthrobotrisins. Results revealed Aoacy, AopkaR, AopkaC1 were involved hyphal growth, trap morphogenesis, sporulation, stress resistance, autophagy. In addition, regulation mitochondrial morphology, thereby affecting energy metabolism, whereas AopkaC2 affected nuclei, Multi-omics results showed regulated multiple metabolic processes. Collectively, these data highlight indispensable development, pathogenicity oligospora, provide insights into mechanisms pathways formation, lifestyle transition.

Language: Английский

Citations

21
Aoatg11 and Aoatg33 are indispensable for mitophagy, and contribute to conidiation, the stress response, and pathogenicity in the nematode-trapping fungus Arthrobotrys oligospora DOI Creative Commons
Xuemei Li, Meichen Zhu, Yankun Liu

et al.

Microbiological Research, Journal Year: 2022, Volume and Issue: 266, P. 127252 - 127252

Published: Nov. 3, 2022

Language: Английский

Citations

20
Peroxin Pex14/17 Is Required for Trap Formation, and Plays Pleiotropic Roles in Mycelial Development, Stress Response, and Secondary Metabolism in Arthrobotrys oligospora DOI Creative Commons
Qianqian Liu, Dongni Li, Na Bai

et al.

mSphere, Journal Year: 2023, Volume and Issue: 8(2)

Published: Feb. 14, 2023

The peroxins encoded by PEX genes involved in peroxisome biogenesis play a crucial role cellular metabolism and pathogenicity fungi. Herein, we characterized filamentous fungus-specific peroxin Pex14/17 the Arthrobotrys oligospora, representative species of nematode-trapping deletion AoPEX14/17 resulted remarkable reduction mycelial growth, conidia yield, trap formation, pathogenicity. Compared with wild-type strain, ΔAopex14/17 mutant exhibited more lipid droplet reactive oxygen accumulation accompanied significant decrease fatty acid utilization tolerance to oxidative stress. Transcriptomic analysis indicated that was regulation metabolism, genetic information processing, environmental processes. In subcellular morphology, number cell nuclei, autophagosomes, Woronin bodies. Metabolic profile showed AoPex14/17 affects biosynthesis secondary metabolites. Yeast two-hybrid assay revealed interacted AoPex14 but not AoPex13. Taken together, our results suggest is main factor for modulating development, A. oligospora. IMPORTANCE Peroxisome (PEX) an important pathogenic However, roles remain largely unknown (NT) Here, provide direct evidence regulates conidiation, autophagy, endocytosis, catalase activity, stress response oxidants, production. Transcriptome metabolic suggested multiple processes metabolism. Therefore, study extends functions genes, which helps elucidate mechanism organelle development formation NT fungi lays foundation efficient nematode biocontrol agents.

Language: Английский

Citations

12
Tools and basic procedures of gene manipulation in nematode-trapping fungi DOI Creative Commons

Shunxian Wang,

Xingzhong Liu

Mycology&#58 An International Journal on Fungal Biology, Journal Year: 2023, Volume and Issue: 14(2), P. 75 - 90

Published: Jan. 10, 2023

Nematode-trapping fungi (NTF) are the majority of carnivorous microbes to capture nematodes through diverse and sophisticated trapping organs derived from hyphae. They can adopt lifestyles in addition saprophytism obtain extra-nutrition nematodes. As a special group fungi, NTF not only excellent model organism for studying lifestyle transition but also natural resources exploring biological control However, mechanism remains poorly understood. Nowadays, omics studies have provided numerous genes pathways that associated with phenotypes traits, which need molecular tools verify. Here, we review development progress gene manipulation NTF, including methodology strategy transformation, random mutagenesis methods target methods. The principle practical approach each method was summarized discussed, basic operational flow tool described. This paper offers clear reference instruction researchers who work on as well other fungi.

Language: Английский

Citations

11
The Arf-GAPs, AoAge1 and AoAge2, regulate diverse cellular processes, conidiation, trap formation, and secondary metabolism in Arthrobotrys oligospora DOI
Le Yang, Xuemei Li, Yuxin Ma

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 285, P. 127779 - 127779

Published: May 24, 2024

Language: Английский

Citations

4
The MADS-box transcription factor AoRlmA is involved in the regulation of mycelium development, conidiation, cell-wall integrity, stress response, and trap formation of Arthrobotrys oligospora DOI Creative Commons
Jiangliu Yang, Wenjie Wang, Yankun Liu

et al.

Microbiological Research, Journal Year: 2022, Volume and Issue: 268, P. 127299 - 127299

Published: Dec. 29, 2022

Language: Английский

Citations

15
AoRab7A interacts with AoVps35 and AoVps41 to regulate vacuole assembly, trap formation, conidiation, and functions of proteasomes and ribosomes in Arthrobotrys oligospora DOI

Yingmei Zhu,

Xuewei Yang, Na Bai

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127573 - 127573

Published: Dec. 12, 2023

Language: Английский

Citations

9
Roles of the Fungal-Specific Lysine Biosynthetic Pathway in the Nematode-Trapping Fungus Arthrobotrys oligospora Identified through Metabolomics Analyses DOI Creative Commons
Hengqian Lu, Shuai Wang, Tiantian Gu

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(2), P. 206 - 206

Published: Feb. 5, 2023

In higher fungi, lysine is biosynthesized via the α-aminoadipate (AAA) pathway, which differs from plants, bacteria, and lower fungi. The differences offer a unique opportunity to develop molecular regulatory strategy for biological control of plant parasitic nematodes, based on nematode-trapping this study, in fungus model Arthrobotrys oligospora, we characterized core gene AAA encoding reductase (Aoaar), sequence analyses through comparing growth, biochemical global metabolic profiles wild-type Aoaar knockout strains. not only has α-aminoadipic acid activity, serves fungal L-lysine biosynthesis, but it also non-ribosomal peptides biosynthetic cluster. Compared with WT, growth rate, conidial production, number predation rings formed, nematode feeding rate ΔAoaar strain were decreased by 40-60%, 36%, 32%, 52%, respectively. Amino metabolism, biosynthesis analogues, phenylpropanoid polyketide lipid metabolism carbon metabolically reprogrammed disruption perturbed intermediates then amino acid-related secondary finally, impeded nematocidal ability A. oligospora. This study provides an important reference uncovering role primary capture confirms feasibility Aoarr as target regulate fungi biocontrol nematodes.

Language: Английский

Citations

5