From “Maraschino” to Cell Cultures: A Deep Study on Prunus cerasus L. Cell Culture Juices DOI Creative Commons

Vanessa Dalla Costa,

Anna Piovan,

Ina Varfaj

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1089 - 1089

Published: Feb. 27, 2025

Prunus cerasus var. Marasca (Rosaceae) is an important Croatian cultivar, known wordwide for the production of Luxardo maraschino liqueur, which occurs in eastern Po Valley Italy. Besides attractive its beneficial effects on human health and well-being. The undifferentiated vitro cell cultures were investigated as a source healthy products. conditions obtaining callus suspension under photoperiod defined. lines evaluated growth rate, total phenol proanthocyanidin contents, antioxidant activities via colorimetric assays. best also subcultured darkness, studying importance light parameter possible industrial scaling-up. juices extracted from obtained biomasses analyzed by LC-DAD-MS six flavanone derivatives, among naringenin glucoside identified. quantitative analysis, pursued during cycle, revealed that content was higher at end cycle (28th day) identified compounds varied 17.22 to 79.22 μg/mL juice. results anti-inflammatory Caco-2 cells support potential applications this material health.

Language: Английский

Light and Plant Growth Regulators on In Vitro Proliferation DOI Creative Commons
Valeria Cavallaro, Alessandra Pellegrino, R. Muleo

et al.

Plants, Journal Year: 2022, Volume and Issue: 11(7), P. 844 - 844

Published: March 22, 2022

Plant tissue cultures depend entirely upon artificial light sources for illumination. The illumination should provide in the appropriate regions of electromagnetic spectrum photomorphogenic responses and photosynthetic metabolism. Controlling quality, irradiances photoperiod enables production plants with desired characteristics. Moreover, significant money savings may be achieved using both more less consuming energy lamps. In this review, attention will focused on effects characteristics plant growth regulators shoot proliferation, main process vitro propagation. balance endogenous also presented. For each spectrum, proliferation but plantlet i.e., length, fresh dry weight photosynthesis, have been analyzed. Even if a huge amount literature is available results are often conflicting. fact, lot exogenous factors, lack common protocol, make it difficult to choose most effective large number species. However, some general issues derived from analysis discussed.

Language: Английский

Citations

72

Plant Growth Regulation in Cell and Tissue Culture In Vitro DOI Creative Commons
Taras Pasternak, Douglas André Steinmacher

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 327 - 327

Published: Jan. 22, 2024

Precise knowledge of all aspects controlling plant tissue culture and in vitro regeneration is crucial for biotechnologists their correlated industry, as there increasing demand this scientific knowledge, resulting more productive resilient plants the field. However, development application cell techniques are usually based on empirical studies, although some data-driven models available. Overall, success dependent several factors such available nutrients, endogenous auxin synthesis, organic compounds, environment conditions. In review, most important described one by one, with practical recommendations basic research physiology sharing our experience from over 20 years The main aim to help new increase impact industry worldwide.

Language: Английский

Citations

51

The Role of Blue and Red Light in the Orchestration of Secondary Metabolites, Nutrient Transport and Plant Quality DOI Creative Commons
Alice Trivellini, Stefania Toscano, Daniela Romano

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(10), P. 2026 - 2026

Published: May 18, 2023

Light is a fundamental environmental parameter for plant growth and development because it provides an energy source carbon fixation during photosynthesis regulates many other physiological processes through its signaling. In indoor horticultural cultivation systems, sole-source light-emitting diodes (LEDs) have shown great potential optimizing producing high-quality products. also regulator of flowering, acting on phytochromes inducing or inhibiting photoperiodic plants. Plants respond to light quality several receptors that can absorb at different wavelengths. This review summarizes recent progress in our understanding the role blue red modulation important traits, nutrient absorption assimilation, as well secondary metabolites, includes dynamic signaling networks are orchestrated by wavelengths with focus transcriptional metabolic reprogramming, productivity, nutritional Moreover, highlights future lines research should increase knowledge develop tailored recipes shape characteristics nutraceutical value

Language: Английский

Citations

35

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm DOI Creative Commons
Elena Popova,

И.Е. Куличенко,

Haeng-Hoon Kim

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(4), P. 542 - 542

Published: April 2, 2023

Cryopreservation is an effective option for the long-term conservation of plant genetic resources, including vegetatively propagated crops and ornamental plants, elite tree genotypes, threatened species with non-orthodox seeds or limited seed availability, as well cell root cultures useful biotechnology. With increasing success, arsenal cryopreservation methods has been developed applied to many material types. However, severe damage accumulating during multi-step procedure often causes reduced survival low regrowth, even when optimized protocol applied. The conditions at recovery stage play a vital role in supporting regrowth after and, optimized, may shift life-and-death balance toward positive outcome. In this contribution, we provide overview five main strategies available improve post-cryopreservation vitro materials their further proliferation development. particular, discuss modification medium composition (iron- ammonium-free), exogenous additives cope oxidative stress absorb toxic chemicals, modulation osmotic potential. Special attention paid growth regulators used various steps process induce desired morphological response cryopreserved tissues. Given studies on electron transport energy provision rewarmed materials, effects light-and-dark light quality. We hope that summary provides helpful guideline set references choosing have not cryopreserved. also propose step-wise be most sensitive cryopreservation-induced chemical stresses.

Language: Английский

Citations

28

Light as an elicitor for enhanced production of secondary metabolites in plant cell, tissue, and organ cultures DOI
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee‐Yoeup Paek

et al.

Plant Growth Regulation, Journal Year: 2024, Volume and Issue: unknown

Published: March 29, 2024

Language: Английский

Citations

14

Light elicited growth, antioxidant enzymes activities and production of medicinal compounds in callus culture of Cnidium officinale Makino DOI
Muhammad Adil, Xiuxia Ren, Byoung Ryong Jeong

et al.

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2019, Volume and Issue: 196, P. 111509 - 111509

Published: May 10, 2019

Language: Английский

Citations

63

Comparative Analysis of Machine Learning and Evolutionary Optimization Algorithms for Precision Micropropagation of Cannabis sativa: Prediction and Validation of in vitro Shoot Growth and Development Based on the Optimization of Light and Carbohydrate Sources DOI Creative Commons
Marco Pepe, Mohsen Hesami,

Finlay Small

et al.

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 21, 2021

Micropropagation techniques offer opportunity to proliferate, maintain, and study dynamic plant responses in highly controlled environments without confounding external influences, forming the basis for many biotechnological applications. With medicinal recreational interests Cannabis sativa L. growing, research related optimization of vitro practices is needed improve current methods while boosting our understanding underlying physiological processes. Unfortunately, due exorbitantly large array factors influencing tissue culture, existing approaches optimize are tedious time-consuming. Therefore, there great potential use new computational methodologies analyzing data develop improved protocols more efficiently. Here, we first tested effects light qualities using assorted combinations Red, Blue, Far White spanning 0-100 μmol/m2/s combination with sucrose concentrations ranging from 1 6% (w/v), totaling 66 treatments, on shoot growth, root development, number nodes, emergence, canopy surface area. Collected were then assessed multilayer perceptron (MLP), generalized regression neural network (GRNN), adaptive neuro-fuzzy inference system (ANFIS) model predict growth development. Based results, GRNN had better performance than MLP or ANFIS was consequently selected link different algorithms [genetic algorithm (GA), biogeography-based (BBO), interior search (ISA), symbiotic organisms (SOS)] prediction optimal levels (quality/intensity) concentration various Predictions conditions refine subsequently a validation experiment showed no significant differences between predicted optimized values observed data. Thus, this demonstrates machine learning most favorable elicit specific developmental responses. these, recommendations carbohydrate promote outcomes suggested. Ultimately, work showcases importance quality supply directing development as well power investigate complex interactions culture.

Language: Английский

Citations

46

Red LED light promotes biomass, flowering and secondary metabolites accumulation in hydroponically grown Hypericum perforatum L. (cv. Topas) DOI

Masumeh Karimi,

Nima Ahmadi,

Morteza Ebrahimi

et al.

Industrial Crops and Products, Journal Year: 2021, Volume and Issue: 175, P. 114239 - 114239

Published: Nov. 19, 2021

Language: Английский

Citations

45

The Role of Light Quality in Regulating Early Seedling Development DOI Creative Commons
Yunmin Wei, Shuwei Wang, Dashi Yu

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(14), P. 2746 - 2746

Published: July 24, 2023

It is well-established that plants are sessile and photoautotrophic organisms rely on light throughout their entire life cycle. Light quality (spectral composition) especially important as it provides energy for photosynthesis influences signaling pathways regulate plant development in the complex process of photomorphogenesis. During previous years, significant progress has been made quality's physiological biochemical effects crops. However, understanding how modulates growth remains a challenge. In this review, we provide an overview role regulating early plants, encompassing processes such seed germination, seedling de-etiolation, establishment. These insights can be harnessed to improve production planning crop by producing high-quality seedlings factories improving theoretical framework modern agriculture.

Language: Английский

Citations

18

Androgenesis in soybean (Glycine max (L.) Merr.): a critical revisit DOI
Ayyagari Ramlal, Sahil Mehta, Aparna Nautiyal

et al.

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2024, Volume and Issue: 60(1), P. 1 - 15

Published: Jan. 4, 2024

Language: Английский

Citations

8