International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127891 - 127891
Published: Nov. 4, 2023
Language: Английский
Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12
Published: April 2, 2024
The tissues or organs derived decellularized extracellular matrix carry immunogenicity and the risk of pathogen transmission, resulting in limited therapeutic effects. cell dECM cultured vitro can address these potential risks, but its impact on wound remodeling is still unclear. This study aimed to explore role (dECM) extracted from adipose stem cells (ADSCs) skin regeneration. Methods: ADSCs were human tissue. Then we cultivated adipose-derived ADSC-dECM for freeze-drying. Western blot (WB), enzyme-linked immunosorbent assay (ELISA) mass spectrometry (MS) conducted analyzed main protein components ADSC-dECM. counting (CCK-8) scratch used effects different concentrations proliferation migration keratinocytes (HaCaT), umbilical vein endothelia (HUVEC) fibroblasts (HFB), respectively. Moreover, designed a novel ADSC-dECM-CMC patch which carboxymethylcellulose (CMC) load with ADSC-dECM; further investigated effect mouse full thickness model. Results: was obtained after decellularization ADSCs. blot, ELISA results showed that contained various bioactive molecules, including collagen, elastin, laminin, growth factors. CCK-8 treatment could significantly promote HaCaT, cells, fibroblasts, To evaluate healing vivo , developed transplanted it into full-thickness And found accelerated closure time. Further histology immunohistochemistry indicated tissue regeneration, as confirmed via enhanced angiogenesis high proliferative activity. Conclusion: In this study, containing multiple molecules exhibiting good biocompatibility reconstruction provides new approach use engineering.
Language: Английский
Citations
5
Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15
Published: July 26, 2024
Diabetic wounds are more complex than normal chronic because of factors such as hypoxia, reduced local angiogenesis, and prolonged inflammation phase. Fibrous proteins, including collagen, fibrin, laminin, fibronectin, elastin etc., possess excellent inherent properties that make them highly advantageous in the area wound healing. Accumulating evidence suggests they contribute to healing process diabetic by facilitating repair remodel extracellular matrix, stimulating development vascular granulation tissue, so on. However, there is currently a lack comprehensive review application these proteins diabetes wounds. An overview fibrous protein characteristics alterations linked given this article's initial section. Next summary advanced applications last five years, acellular dermal hydrogel, foam, scaffold, electrospun nanofibrous membrane. These dressings have ability actively promote addition just covering compared traditional like gauze or bandage. Research on their role may result novel therapeutic modalities lower incidence thereby enhance health patients.
Language: Английский
Citations
5
American Journal of Cardiovascular Drugs, Journal Year: 2024, Volume and Issue: 24(6), P. 707 - 718
Published: Aug. 24, 2024
Language: Английский
Citations
5
Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 175, P. 55 - 75
Published: Dec. 22, 2023
With the lack of minimally invasive tools for probing neuronal systems across spatiotemporal scales, understanding working mechanism nervous system and limited assessments available are imperative to prevent or treat neurological disorders. In particular, nanoengineered neural interfaces can provide a solution this technological barrier. This review covers recent surface engineering approaches, including nanoscale coatings, range topographies from microscale nanoscale, primarily focusing on neural-interfaced biosystems. Specifically, immobilization bioactive molecules fertilize cell lineage, topographical induce mechanotransduction in cells, enhanced cell-chip coupling using three-dimensional structured surfaces highlighted. Advances interface design will help us understand system, thereby achieving effective treatments disorders.\ • focuses designing with chemical modification at multiscale perspective. Versatile coatings summarized. Recent advances materials applicable culture, electrophysiological sensing, implants reviewed.
Language: Английский
Citations
13
Biomaterials, Journal Year: 2023, Volume and Issue: 302, P. 122348 - 122348
Published: Oct. 14, 2023
The hair follicle (HF) is a multicellular complex structure of the skin that contains reservoir multipotent stem cells. Traditional repair methods such as drug therapies, transplantation, and cell therapy have limitations. Advances in nanotechnology offer new approaches for HF regeneration, including controlled release HF-specific targeting. Until recently, embryogenesis was thought to be only mechanism forming follicles. However, recent years, phenomenon wound-induced neogenesis (WIHN) or de novo regeneration has gained attention it can occur under certain conditions wound beds. This review covers targeting strategies, with particular emphasis on currently used nanotechnology-based strategies both loss-related diseases regeneration. discussed several modalities: modulation cycle, stimulation progenitor cells signaling pathways, tissue engineering, WIHN, gene therapy. been identified an ideal target some regulatory challenges may delay development based-strategies, which will lastly discussed.
Language: Английский
Citations
11
Journal of Biological Engineering, Journal Year: 2024, Volume and Issue: 18(1)
Published: Feb. 22, 2024
Abstract Background The extracellular matrix (ECM) is a three-dimensional network of proteins that encases and supports cells within tissue promotes physiological pathological cellular differentiation functionality. Understanding the complex composition ECM essential to decrypt processes as well pathogenesis. In this context, method decellularization useful technique eliminate components from tissues while preserving majority structural functional integrity ECM. Results study, we employed bottom-up proteomic approach elucidate intricate in decellularized matrices murine liver kidney tissues. This involved use novel, perfusion-based protocol generate acellular whole organ scaffolds. Proteomic analysis mice scaffolds revealed tissue-specific differences matrisome composition, found predominantly stable core matrisome, consisting collagens, glycoproteins, proteoglycans. Liver unique such collagen type VI alpha-6, fibrillin-2 or biglycan. kidney, specific ECM-regulators cathepsin z were detected. Conclusion identification distinct signatures provides insights into how different compositions might influence biological properties experimental workflow will help further landscape order decipher cell–matrix interactions their contribution microenvironment.
Language: Английский
Citations
4
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: March 21, 2024
Abstract Extracellular matrix (ECM) is an intricate structure providing the microenvironment niche that influences stem cell differentiation. This study aimed to investigate efficacy of decellularized ECM derived from human dental pulp cells (dECM_DPSCs) and gingival-derived mesenchymal (dECM_GSCs) as inductive scaffold for osteogenic differentiation GSCs. The proteomic analysis demonstrated common signature matrisome proteins dECM_DPSCs dECM_GSCs were related osteogenesis/osteogenic RNA sequencing data GSCs reseeded on revealed upregulated genes Hippo Wnt signaling pathways in In inhibitor experiments, results superiorly promoted differentiation, mainly mediated through signaling. present emphasizes promising translational application a bio-scaffold rich favorable regenerative tissue engineering.
Language: Английский
Citations
4
ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Perianal fistulas (PAFs) are a severe complication of Crohn's disease that significantly impact patient prognosis and quality life. While stem-cell-based strategies have been widely applied for PAF treatment, their efficacy remains limited. Our study introduces an injectable, temperature-controlled decellularized adipose tissue-alginate hydrogel loaded with dental pulp mesenchymal stem cells (DPMSCs) in vivo fistula treatment. The experimental group demonstrated higher healing rates compared to surgical DPMSCs groups, as evidenced by magnetic resonance imaging, multiplex immunohistochemical, ELISA analyses. KEGG enrichment differential genes suggested cellular senescence involvement cell therapy efficacy, further confirmed β-galactosidase staining markers (p21 p53). Collectively, our research provides novel PAFs illuminates underlying mechanisms.
Language: Английский
Citations
0
Journal of Tissue Viability, Journal Year: 2025, Volume and Issue: 34(2), P. 100870 - 100870
Published: Feb. 8, 2025
More than 5 % of people worldwide suffer from hearing disorders. Ototoxic drugs, aging, exposure to loud sounds, rupture, subperichondrial hematoma, perichondritis, burns and frostbite infections are the main causes loss, some which can destroy cartilage lead deformation. On other hand, disorders external ear diverse range dangerous neoplasms defects that not acceptable a cosmetic standpoint. These issues include injuries, blockages, dermatoses, infections, any or all them may be bothersome busy doctor. Using an implant aid is one treatment strategies for deafness. However, these medical devices useful every eligible patient. With right therapy, many life-threatening treated with confidence in positive outcome. As research have advanced dramatically past ten years, tissue engineering (TE) has emerged as promising method regenerate damaged tissue, raising prospect permanent cure Decellularization now seen development regenerative medicine, increasing number applications being found acellular matrices. Studies on decellularization show natural scaffolds made decellularized tissues serve suitable platform while preserving components, preparation such will important part future bioscience research. It wide medicine TE. This review intends give overview status alternative inner outer both preclinical clinical perspective order how ongoing TE potential advance enhance novel disease treatments.
Language: Английский
Citations
0
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)
Published: Feb. 1, 2025
ABSTRACT The global prevalence of skeletal muscle diseases has progressively escalated in recent years. This study aimed to explore the potential role matrix stiffness repair mechanisms following injury. We observed an increase stiffness, a significant rise number type I fibres and notable elevation mRNA expression levels Myh7/2 alongside decrease Myh1/4 on day 3 post tibialis anterior To replicate these vivo changes, C2C12 cells were cultured under high conditions, compared those low showed increased Myh7 / 2 production MYH7/2, indicating differentiation into slow‐twitch fibre types. Furthermore, up‐regulation DRP1 phosphorylation along with elevated F‐actin fluorescence intensity RHOA ROCK1 indicates that induces cytoskeletal remodelling regulate mitochondrial fission processes. Our data also revealed level for Actb , DRP1, quantity MYH7/2 level. Importantly, effects effectively reversed by application ROCK inhibitor Y‐27632, highlighting targeting dynamics can modulate myogenic pathways within cells. These findings provide valuable insights how influences transformation during injury while suggesting therapeutic targets intervention.
Language: Английский
Citations
0