CURRENT PERSPECTIVES ON THE EFFECTS OF MAIN TYPES OF CARRAGEENANS ON THE HUMAN BODY DOI Open Access

R. O. Bachynskyi,

Оксана Анатоліївна Наконечна, S. Stetsenko

et al.

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(4), P. 309 - 314

Published: Dec. 26, 2024

The relevance of studying carrageenans (red seaweed extracts) stems from the need to address a medical and scientific issue concerning limited depth understanding regarding their effects on human body. purpose this study is analyze current literature primary types carrageenans—kappa, iota, lambda—and evaluate safety efficacy these food additives in industry. Results discussion. Due hydrocolloidal properties, contribute formation stable structures various matrices, making them key ingredient production dairy meat products, as well products made plant ingredients. On other hand, due structural functional diversity, are used cosmetics pharmaceutical industries. According modern research, external use biopolymers considered safe. Moreover, experimental studies have shown that antioxidant demonstrate wide range antiviral effects, effective against viruses, including SARS-CoV-2, dengue virus, herpes simplex cytomegalovirus, HIV, rabies papillomavirus, influenza rhinoviruses. It has been established different dietary undegraded can significantly affect gastrointestinal tract, namely, digestion processes - proteolysis. In vitro results demonstrated inhibit activity enzymes such gastric pepsin pancreatic trypsin. One potential toxic orally consuming E407 E407a disruption protein leading deficiency essential amino acids inhibition To gain deeper further in-depth needed explore biochemical mechanisms by which investigate dose-dependent nature changes.

Language: Английский

Detecting changes of testicular interstitial cell membranes with a fluorescent probe after incubation and cryopreservation with cryoprotective agents DOI
Oleksandr Pakhomov, Yevgen O. Posokhov

Cryobiology, Journal Year: 2025, Volume and Issue: 118, P. 105194 - 105194

Published: Jan. 6, 2025

Language: Английский

Citations

0
The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration DOI Creative Commons
Liudmyla Sukhodub, Mariia Kumeda, Л. Ф. Суходуб

et al.

Carbohydrate Polymer Technologies and Applications, Journal Year: 2024, Volume and Issue: 8, P. 100528 - 100528

Published: June 10, 2024

Language: Английский

Citations

2
The Effect of Carbon and Magnetic Nanoparticles on the Properties of Chitosan-Based Neural Tubes: Cytotoxicity, Drug Release, in Vivo Nerve Regeneration DOI
Liudmyla Sukhodub, Mariia Kumeda, Л. Ф. Суходуб

et al.

Published: Jan. 1, 2024

This study aims to determine the effect of graphene oxide (GO), multilayer carbon tubes (MWCNTs+Fe), magnetite (Fe3O4) and in situ forming brushite (DCPD) nanoparticles (NPs) chitosan (CS) based matrices on cytotoxicity, Pregabalin (PG) release, vivo behaviour mechanically stabilized, conductive nerve conduits (NCs). NPs change composites' hydrophilicity order: DCPD (46.8) ˂ MWCNT (54.21) ~ Fe3O4 (54.44) GO (65.99) water contact angle (47-66 degrees) corresponds conditions cell adhesion. ≤ 150 μg/ml reduce adhesion but don't trigger oxidative stress toxic effects. Magnetite 300 µg/ml promote apoptosis cells. The stage PG introduction into NCs determines its release kinetic: formation provides uniform drug release. most electrically GO- MWCNTs+Fe - containing have a prolonged biodegradation period about 6 months, which makes them promising for regeneration damaged peripheral nerves humans.

Language: Английский

Citations

1
Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro DOI
Volodymyr Prokopіuk, Anatolii Onishchenko,

Yuliia Pazura

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Abstract Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity newly synthesized nanostructured carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity microflakes was against murine L929 cell line rat mature erythrocytes. MTT neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) Fura 2-AM staining reactive nitrogen species (RNS), detection caspases, lipid order-sensitive NR12S probe were employed evaluate viability, motility, death pathways, implication Ca2+, oxygen (ROS) RNS signaling, alterations membranes following exposure microflakes, respectively. To assess hemocompatibility Zn-containing osmotic fragility assay hemolysis performed, well multiple eryptosis parameters evaluated. Results. Our findings indicate dose-response ZnCH cells with no toxicity observed low concentrations (10 mg/L below). At high concentrations, promoted oxidative stress, Ca2+- caspase-dependent apoptosis altered membranes. Surprisingly, enucleated erythrocytes found lower compared cells. induced associated Ca2+ signaling recruitment caspases. Eryptosis assays more sensitive than evaluation hemolysis. Conclusions. show at indicating their potential

Language: Английский

Citations

0
Eryptosis contributes to gestational diabetes mellitus in maternal obesity DOI
В. В. Лазуренко, O.Yu. Zhelezniakov, Volodymyr Prokopіuk

et al.

Inter Collegas, Journal Year: 2024, Volume and Issue: 11(3)

Published: Sept. 30, 2024

Background. Obesity is considered to be a risk factor for Gestational Diabetes Mellitus (GDM), which characterised by hyperglycaemia in pregnant women. Recent studies have demonstrated that glycated erythrocytes are more prone eryptosis, unique regulated cell death observed only mature erythrocytes. The aim of the current study was analyse how eryptosis might contribute GSM maternal obesity. Materials & Methods. Eryptosis parameters were assessed women enrolled study: group 1 consisted 12 obese without signs GDM, 15 obesity but with GDM included 2, 14 3, 4 (control) obstetric and extragenital pathology. Phosphatidylserine externalisation flow cytometry following Annexin V-FITC staining circulating isolated from Additionally, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) used oxidative stress Results. Groups 1, 2 3 showed higher degree ROS-dependent compared control (IV). There no statistically significant difference (p>0.05) between groups 2. However, percentage phosphatidylserine-dispersed level ROS significantly Conclusions. associated accompanied accelerated eryptosis. Enhanced act as an additional contributing thrombosis endothelial dysfunction GDM. Keywords: pregnancy, phosphatidylserine, cytometry, Annexin,

Language: Английский

Citations

0
CURRENT PERSPECTIVES ON THE EFFECTS OF MAIN TYPES OF CARRAGEENANS ON THE HUMAN BODY DOI Open Access

R. O. Bachynskyi,

Оксана Анатоліївна Наконечна, S. Stetsenko

et al.

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(4), P. 309 - 314

Published: Dec. 26, 2024

The relevance of studying carrageenans (red seaweed extracts) stems from the need to address a medical and scientific issue concerning limited depth understanding regarding their effects on human body. purpose this study is analyze current literature primary types carrageenans—kappa, iota, lambda—and evaluate safety efficacy these food additives in industry. Results discussion. Due hydrocolloidal properties, contribute formation stable structures various matrices, making them key ingredient production dairy meat products, as well products made plant ingredients. On other hand, due structural functional diversity, are used cosmetics pharmaceutical industries. According modern research, external use biopolymers considered safe. Moreover, experimental studies have shown that antioxidant demonstrate wide range antiviral effects, effective against viruses, including SARS-CoV-2, dengue virus, herpes simplex cytomegalovirus, HIV, rabies papillomavirus, influenza rhinoviruses. It has been established different dietary undegraded can significantly affect gastrointestinal tract, namely, digestion processes - proteolysis. In vitro results demonstrated inhibit activity enzymes such gastric pepsin pancreatic trypsin. One potential toxic orally consuming E407 E407a disruption protein leading deficiency essential amino acids inhibition To gain deeper further in-depth needed explore biochemical mechanisms by which investigate dose-dependent nature changes.

Language: Английский

Citations

0