PLK2 inhibited oxidative stress and ameliorated hepatic ischemia–reperfusion injury through phosphorylating GSK3β DOI

Wenwen Ge,

Zhoucheng Wang, Xinyang Zhong

et al.

Journal of Gastroenterology and Hepatology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Background and Aim Hepatic ischemia–reperfusion (I/R) injury is the primary cause of liver dysfunction failure, commonly occurring in transplantation, hepatectomy, hemorrhagic shock. Polo‐like kinase 2 (PLK2), a pivotal regulator centriole duplication, plays crucial role cell proliferation repair. However, function PLK2 hepatic I/R remains unclear. Methods The effect was investigated mouse model hepatocyte hypoxia‐reoxygenation (H/R) model. Liver assessed by serum transaminase hematoxylin eosin staining. Cell apoptosis analyzed using TUNEL analysis immunoblotting. Inflammatory factors were evaluated reverse transcription‐quantitative polymerase chain reaction. Mice or cultured cells during H/R treated overexpressing PLK2. ROS fluorescence staining used to assess oxidative stress injury. Results upregulated after Overexpressed significantly improved enzyme levels alleviated histological Moreover, decreased inhibited expression inflammatory liver. Mechanistically, increased phosphorylation GSK3β enhanced antioxidant HO‐1, leading less production. Inhibition HO‐1 aggravated generation abolished protective Conclusion Overall, these findings revealed that reduced damage injury, this related activity.

Language: Английский

Iron chelators loaded on myocardiocyte mitochondria-targeted nanozyme system for treating myocardial ischemia-reperfusion injury in mouse models DOI Creative Commons
Ke Zhu, Kun Wang,

Rongting Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 15, 2025

Ferroptosis plays a critical role in myocardial ischemia-reperfusion injury (MIRI), posing significant clinical challenge. Nanoenzymes like cerium oxide (CeO2) hold promise for mitigating oxidative damage and inhibiting ferroptosis, but their delivery efficiency biological activity require optimization. This study aims to develop targeted nanozyme system MIRI treatment by integrating CeO2 with mesoporous polydopamine (mPDA) dexrazoxane (DXZ) achieve synergistic therapeutic effects. A biomineralization technique was used synthesize nanoparticles (2–3 nm) within mPDA, forming ~ 130 nm composite (Ce@mPDA). Surface modifications cardiac homing peptide (CHP) triphenylphosphine (TPP) enabled hierarchical targeting injured myocardium mitochondria. DXZ-loaded Ce@mPDA-C/P (D/Ce@mPDA-C/P) were evaluated vitro mouse model effects on stress, apoptosis, inflammation, function. D/Ce@mPDA-C/P exhibited robust ROS scavenging, sustained DXZ release, efficient mitochondrial targeting. The significantly reduced upregulated GPX4 expression, inhibited modulated the inflammatory microenvironment. Long-term studies demonstrated reductions fibrosis improvements function, including enhanced fractional shortening ejection fraction. effectively combines antioxidant properties of iron-chelating DXZ, providing promising strategy MIRI. approach may expand use advance nanomedicine-based interventions repair.

Language: Английский

Citations

1
p16INK4a Aggravated Sepsis-associated Cardiac Injury by Inhibiting the PI3K/AKT Pathway and Inducing Redox Imbalance DOI
Baihong Li, Wei Wang, Xiaoyan Wang

et al.

Journal of Cardiovascular Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Language: Английский

Citations

0
MiR-222-3p loaded stem cell nanovesicles repair myocardial ischemia damage via inhibiting mitochondrial oxidative stress DOI Creative Commons
Mei Liu, Le Wang, Zhao Liu

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123447 - 123447

Published: Feb. 1, 2025

Language: Английский

Citations

0
Cardioprotective potential of transcription factor PRRX1 silencing against myocardial ischemia/reperfusion injury by regulating excessive mitophagy and ferroptosis through FKBP5-p38 MAPK axis DOI

Yongpeng Fang,

Xudong Niu,

Weifang Zhao

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2025, Volume and Issue: 1871(5), P. 167766 - 167766

Published: March 3, 2025

Language: Английский

Citations

0
Danqi soft caspule alleviates myocardial ischemia-reperfusion injury induced cardiomyocyte apoptosis by attenuating mitochondrial fission DOI Creative Commons
Ye Yang,

Cuiting Lin,

Yan Wang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Background Myocardial ischemia-reperfusion (I/R) injury which leads to continuously worsening ventricular remodeling and cardiac dysfunction in the chronic stage, is a significant contributor global prevalence of heart failure. Traditional Chinese herbal formulas have been shown prevent myocardial I/R injury. Method This study aims investigate whether Danqi soft caspule (DQ), classical traditional medicine (TCM) preparation, exerted protective effects against explore potential underlying mechanisms. A rat model cell H 2 O induced oxidative stress were established assess DQ on injury, cardiomyocyte apoptosis, as well mitochondrial structure function. Result pre-treatment reduced both proportion infarct area ischemic risk decreased apoptosis rats. In cells, was found reduce lower levels. Furthermore, inhibited fission, prevented alterations membrane potential, suppressed Cytochrome C release from mitochondria, thereby preventing apoptosis. has by reducing through inhibition fission. Moreover, could restore function suppressing phosphorylation Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) dynamin-related 1 (Drp-1). Conclusion fission associated with CaMKII Drp-1.

Language: Английский

Citations

0
The protective role of cannabidiol in stress-induced liver injury: modulating oxidative stress and mitochondrial damage DOI Creative Commons
C. F. Huang,

Huichao Liang,

Xiaohua Liang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 14, 2025

Stress-induced liver injury, resulting from acute or chronic stress, is associated with oxidative stress and inflammation. The endocannabinoid system, particularly cannabinoid receptor 2 (CB2R), plays a crucial role in damage. However, there are currently no clinical drugs targeting CB2R for diseases. Cannabidiol (CBD), agonist, possesses anti-inflammatory antioxidant properties. This study aims to investigate the pharmacological effects of CBD mouse model stress-induced injury. We employed injury evaluate protective CBD. Assessments included histopathological analysis, cytokine detection via ELISA, protein expression analysis using immunohistochemistry Western blot, gene transcription differential analysis. Transmission electron microscopy was utilized observe mitochondrial morphology. Additionally, we examined levels CB2R, SLC7A11, α-SMA, ACSL4 proteins elucidate mechanisms underlying CBD's effects. exhibited significant against mice. Decreases function indicators (including Aspartate Aminotransferase (AST) Alanine (ALT)) inflammatory cytokines (such as IL-1β Tumor Necrosis Factor-alpha (TNF-α)) were observed. enhanced reduced α-SMA levels, mitigating fibrosis. It also decreased increased SOD GSH-Px activities, upregulated SLC7A11 expression. Furthermore, improved morphology, indicating reduction cell death. activates CB2R/α-SMA pathway modulate inflammation Through SLC7A11/ACSL4 signaling pathway, alleviates enhances reduces These findings provide theoretical basis potential application prevention treatment

Language: Английский

Citations

0
Ferulic acid protects against stress-induced myocardial injury in mice DOI

Siyong Li,

Peiyi He,

Jiahe Liu

et al.

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: 498, P. 117309 - 117309

Published: March 20, 2025

Language: Английский

Citations

0
Reactive oxygen species (ROS)-responsive biomaterials for treating myocardial ischemia-reperfusion injury DOI Creative Commons
Ying Zhang,

Mantang Jiang,

Tao Wang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 2, 2024

Myocardial ischemia-reperfusion injury (MIRI) is a critical issue that arises when restoring blood flow after an ischemic event in the heart. Excessive reactive oxygen species (ROS) production during this process exacerbates cellular damage and impairs cardiac function. Recent therapeutic strategies have focused on leveraging ROS microenvironment to design targeted drug delivery systems. ROS-responsive biomaterials emerged as promising candidates, offering enhanced efficacy with reduced systemic adverse effects. This review examines mechanisms of overproduction myocardial summarizes significant advancements for MIRI treatment. We discuss various chemical impart sensitivity these materials, emphasizing ROS-induced solubility switches degradation mechanisms. Additionally, we highlight platforms, such nanoparticles hydrogels, their unique advantages MIRI. Preclinical studies demonstrating materials mitigating animal models are reviewed, alongside action potential clinical implications. also address challenges future prospects translating state art biomaterial-based therapeutics into practice improve management outcomes. will provide valuable insights researchers clinicians working novel intervention.

Language: Английский

Citations

3
Oxidative Stress and Inflammation in Myocardial Ischemia–Reperfusion Injury: Protective Effects of Plant‐Derived Natural Active Compounds DOI
Jia Chen, Boyu Wang, Tianwei Meng

et al.

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

ABSTRACT Acute myocardial infarction (AMI) remains a leading cause of death among patients with cardiovascular diseases. Percutaneous coronary intervention (PCI) has been the preferred clinical treatment for AMI due to its safety and efficiency. However, research indicates that rapid restoration oxygen supply following PCI can lead secondary injury, termed ischemia–reperfusion injury (MIRI), posing grave threat patient survival. Despite ongoing efforts, mechanisms underlying MIRI are not yet fully elucidated. Among them, oxidative stress inflammation stand out as critical pathophysiological mechanisms, playing significant roles in MIRI. Natural compounds have shown strong therapeutic potential their high efficacy, availability, low side effects. Many current studies indicate natural mitigate by reducing inflammatory responses. Therefore, this paper reviews during role intervening these processes, aiming provide basis reference future development drugs treating

Language: Английский

Citations

0
PLK2 inhibited oxidative stress and ameliorated hepatic ischemia–reperfusion injury through phosphorylating GSK3β DOI

Wenwen Ge,

Zhoucheng Wang, Xinyang Zhong

et al.

Journal of Gastroenterology and Hepatology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Background and Aim Hepatic ischemia–reperfusion (I/R) injury is the primary cause of liver dysfunction failure, commonly occurring in transplantation, hepatectomy, hemorrhagic shock. Polo‐like kinase 2 (PLK2), a pivotal regulator centriole duplication, plays crucial role cell proliferation repair. However, function PLK2 hepatic I/R remains unclear. Methods The effect was investigated mouse model hepatocyte hypoxia‐reoxygenation (H/R) model. Liver assessed by serum transaminase hematoxylin eosin staining. Cell apoptosis analyzed using TUNEL analysis immunoblotting. Inflammatory factors were evaluated reverse transcription‐quantitative polymerase chain reaction. Mice or cultured cells during H/R treated overexpressing PLK2. ROS fluorescence staining used to assess oxidative stress injury. Results upregulated after Overexpressed significantly improved enzyme levels alleviated histological Moreover, decreased inhibited expression inflammatory liver. Mechanistically, increased phosphorylation GSK3β enhanced antioxidant HO‐1, leading less production. Inhibition HO‐1 aggravated generation abolished protective Conclusion Overall, these findings revealed that reduced damage injury, this related activity.

Language: Английский

Citations

0