Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ_25-35 in a cellular model of Alzheimer’s disease

Cell Cycle, Journal Year: 2021, Volume and Issue: 20(1), P. 112 - 125

Published: Jan. 2, 2021

Accumulating data manifest that long non-coding RNA (lncRNAs) are involved in all kinds of neurodegenerative disorders, consisting the onset and progression Alzheimer's disease (AD). The study was for research mechanism lncRNA H19 (H19) viability apoptosis PC12 cells induced by Aβ25-35 a cellular model AD with regulation microRNA (miR)-129 high mobility group box-1 protein (HMGB1). An established using Aβ25-35. Aβ25-35-induced were transfected si-H19 or miR-129 mimic to figure their roles cell viability,apoptosis, mitochondrial membrane potential dysfunction oxidative stress AD. Luciferase reporter assay RNA-pull down employed verification binding relationship between targeting HMGB1. mouse brain tissues collected. H19, HMGB1 detected Aβ25-35-treated mice. Elevated decreased found as well Silenced elevated promoted viability, inhibited apoptosis, prevented cells. could specifically bind miR-129. MiR-129 suppressed expression. This suggests silenced up-regulated accelerates represses stimulated AD, which is beneficial treatment.

Language: Английский

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MicroRNAs as Potential Orchestrators of Alzheimer's Disease-Related Pathologies: Insights on Current Status and Future Possibilities

Frontiers in Aging Neuroscience, Journal Year: 2021, Volume and Issue: 13

Published: Oct. 12, 2021

Alzheimer's disease (AD) is a progressive and deleterious neurodegenerative disease, strongly affecting the cognitive functions memory of seniors worldwide. Around 58% affected patients live in low middle-income countries, with estimates increasing deaths caused by AD coming decade. multifactor pathology. Mitochondrial function declines brain currently emerging as hallmark this disease. It has been considered one intracellular processes severely compromised AD. Many mitochondrial parameters decline already during aging; efficiency for energy production, reactive oxygen species (ROS) metabolism de novo synthesis pyrimidines, to reach an extensive functional failure, concomitant onset conditions. Besides its impact on functions, characterized loss synapses, extracellular amyloid plaques composed amyloid-β peptide (Aβ), aggregates hyperphosphorylated Tau protein, accompanied drastic sleep disorders, sensory alterations pain sensitization. Unfortunately, till date, effective management AD-related disorders early, non-invasive diagnostic markers are yet be found. MicroRNAs (miRNAs) small non-coding nucleic acids that regulate key signaling pathway(s) various About 70% experimentally detectable miRNAs expressed where they neurite outgrowth, dendritic spine morphology, synaptic plasticity. Increasing studies suggest intimately involved specific signals formation. This pivotal considering crucial molecules studied dysfunctions increasingly acknowledged contributor via deregulating genes pathogenesis. Moreover, have proved control sensitization circadian clock system affects process. Interestingly, differential expression miRNA panels implies their potential biomarkers. In review, we will present updated analysis role regulating pathologies. We discuss current challenges against wider use future promising capabilities therapeutic means better

Language: Английский

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Bioinformatics Analysis of Next Generation Sequencing Data Identifies Molecular Biomarkers Associated With Type 2 Diabetes Mellitus

Clinical Medicine Insights Endocrinology and Diabetes, Journal Year: 2023, Volume and Issue: 16

Published: Jan. 1, 2023

Type 2 diabetes mellitus (T2DM) is the most common metabolic disorder. The aim of present investigation was to identify gene signature specific T2DM.

Language: Английский

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YAP in development and disease: Navigating the regulatory landscape from retina to brain

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 175, P. 116703 - 116703

Published: May 6, 2024

The distinctive role of Yes-associated protein (YAP) in the nervous system has attracted widespread attention. This comprehensive review strategically uses retina as a vantage point, embarking on an extensive exploration YAP's multifaceted impact from to brain development and pathology. Initially, we explore crucial roles YAP embryonic cerebral development. Our focus then shifts retinal development, examining detail regulatory influence pigment epithelium (RPE) progenitor cells (RPCs), its significant effects hierarchical structure functionality retina. We also investigate essential contributions maintaining homeostasis, highlighting precise regulation cell proliferation survival. In terms retinal-related diseases, epigenetic connections pathophysiological diabetic retinopathy (DR), glaucoma, proliferative vitreoretinopathy (PVR). Lastly, broaden our brain, emphasizing research paradigm "retina: window brain." Special is given emerging studies disorders such Alzheimer's disease (AD) Parkinson's (PD), underlining potential therapeutic value neurodegenerative neuroinflammation.

Language: Английский

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Crocin ameliorates neuroinflammation and cognitive impairment in mice with Alzheimer's disease by activating PI3K/AKT pathway

Brain and Behavior, Journal Year: 2024, Volume and Issue: 14(5)

Published: May 1, 2024

Abstract Background Crocin has a good prospect in the treatment of Alzheimer's disease (AD), but mechanisms underlying its neuroprotective effects remain elusive. This study aimed to investigate and AD. Methods AD mice were set up by injecting Aβ 25‐35 solution into hippocampus. Then, injected intraperitoneally with 40 mg/kg/day for 14 days. Following completion treatment, an open‐field test, Y‐maze test Morris water maze conducted evaluate impact on spatial learning memory deficiency mice. The hippocampal neuron injury, proinflammatory cytokine expressions (IL‐1β, IL‐6, TNF‐α), PI3K/AKT signaling‐related protein measured using hematoxylin eosin staining, Western blot, quantitative real‐time polymerase chain reaction (qRT‐PCR) experiments, respectively. Results attenuated ‐induced injury. Furthermore, blot qRT‐PCR results showed that effectively suppressed inflammation activated pathway Conclusion restrained neuroinflammation via activation pathway, thereby ameliorating cognitive dysfunction

Language: Английский

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Dexmedetomidine Ameliorates Postoperative Cognitive Dysfunction via the MicroRNA-381-Mediated EGR1/p53 Axis

Molecular Neurobiology, Journal Year: 2021, Volume and Issue: 58(10), P. 5052 - 5066

Published: July 10, 2021

Language: Английский

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Alzheimer Disease: Recent Updates on Apolipoprotein E and Gut Microbiome Mediation of Oxidative Stress, and Prospective Interventional Agents

Aging and Disease, Journal Year: 2022, Volume and Issue: 13(1), P. 87 - 87

Published: Jan. 1, 2022

Alzheimer's disease (AD) is a current public health challenge and will remain until the development of an effective intervention. However, developing treatment for requires thorough understanding its etiology, which currently lacking. Although several studies have shown association between oxidative damage AD, only few clarified specific mechanisms involved. Herein, we reviewed recent preclinical clinical that indicated significance in as well potential antioxidants. factors regulate stress centered our investigation on apolipoprotein E gut microbiome. Apolipoprotein E, particularly E-ε4, can impair structural facets mitochondria. This, turn, minimize mitochondrial functionality result progressive build-up free radicals, eventually leading to stress. Similarly, microbiome influence significant degree via metabolite, trimethylamine N-oxide. Given various roles these two modulating stress, also discuss possible relationship them provide future research directions.

Language: Английский

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The Role of miRNAs in Dexmedetomidine’s Neuroprotective Effects against Brain Disorders

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(10), P. 5452 - 5452

Published: May 13, 2022

There are limited neuroprotective strategies for various central nervous system conditions in which fast and sustained management is essential. Neuroprotection-based therapeutics have become an intensively researched topic the neuroscience field, with multiple novel promising agents, from natural products to mesenchymal stem cells, homing peptides, nanoparticles-mediated all aiming significantly provide neuroprotection experimental clinical studies. Dexmedetomidine (DEX), α2 agonist commonly used as anesthetic adjuvant sedation opioid-sparing medication, stands out this context due its well-established effects. Emerging evidence preclinical studies suggested that DEX could be protect against cerebral ischemia, traumatic brain injury (TBI), spinal cord injury, neurodegenerative diseases, postoperative cognitive disorders. MicroRNAs (miRNAs) regulate gene expression at a post-transcriptional level, inhibiting translation of mRNA into functional proteins. In vivo vitro deciphered brain-related miRNAs dysregulated miRNA profiles after several disorders, including TBI, ischemic stroke, Alzheimer's disease, sclerosis, providing emerging new perspectives therapy by modulating these miRNAs. Experimental revealed some effects mediated miRNAs, counteracting mechanisms disease models, such lipopolysaccharides induced neuroinflammation, β-amyloid dysfunction, ischemic-reperfusion anesthesia-induced neurotoxicity models. This review aims outline disorders We address targeting ameliorating anesthetics, reducing improving diseases.

Language: Английский

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Identification of novel hub genes for Alzheimer’s disease associated with the hippocampus using WGCNA and differential gene analysis

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: March 7, 2024

Background Alzheimer’s disease (AD) is a common, refractory, progressive neurodegenerative disorder in which cognitive and memory deficits are highly correlated with abnormalities hippocampal brain regions. There still lack of hippocampus-related markers for AD diagnosis prevention. Methods Differently expressed genes were identified the gene expression profile GSE293789 region. Enrichment analyses GO, KEGG, GSEA used to identify biological pathways involved DEGs AD-related group. WGCNA was modules that associated samples. The intersecting extracted top ten ranked hub identified. Finally GES48350 as validation cohort predict diagnostic efficacy genes. Results From GSE293789, 225 identified, mainly calcium response, glutamatergic synapses, calcium-dependent phospholipid-binding response. analysis yielded dark green bright yellow modular most relevant AD. these two modules, 176 extracted, taken be intersected DEGs, yielding 51 Then 10 them: HSPA1B, HSPB1, HSPA1A, DNAJB1, HSPB8, ANXA2, ANXA1, SOX9, YAP1, AHNAK. Validation found have excellent performance. Conclusion Ten hippocampus contributing further understanding development targets therapeutic

Language: Английский

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MicroRNA-140 silencing represses the incidence of Alzheimer's disease

Neuroscience Letters, Journal Year: 2021, Volume and Issue: 758, P. 135674 - 135674

Published: Jan. 30, 2021

Language: Английский

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