Optimization of Aqueous Extraction Conditions for Bioactive Compounds and Antioxidant Properties of Overripe Banana (Musa acuminata) Using Response Surface Methodology

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101775 - 101775

Published: Feb. 1, 2025

Language: Английский

---

Novel kombucha beverages with antioxidant activity based on fruits as alternative substrates

LWT, Journal Year: 2023, Volume and Issue: 189, P. 115482 - 115482

Published: Nov. 1, 2023

The consumption of kombucha drinks has remarkably grown during the last decade and, besides traditional ingredients such as tea leaves, alternative raw materials with interesting functional properties are being proposed. aim this study was to obtain kombuchas enriched in phenolic compounds and a high antioxidant activity using fruits instead determine optimal fermentation time. fruit-based were prepared cherry, plum, apricot, strawberry, persimmon, grape, orange, or pomegranate three different microbial consortia (SCOBYs/SCs). pH, carbohydrate, ethanol, levels, vitro capacity determined along process (21 days). obtained products showed safe pH values (2.5–4.2) suitable ethanol levels (<1.2%). Their carbohydrate content almost half that commercial kombucha. fermentative microorganisms increased total (TPC) radical scavenging (TEAC) all preparations, differences found depending on SC used. Maximum TPC TEAC observed strawberry (up 13.7 mg/100 mL 2.05 μmol/mL, respectively). results fruits, mainly strawberries, could be used activity.

Language: Английский

---

Bioactive Compound Extraction of Hemp (Cannabis sativa L.) Leaves through Response Surface Methodology Optimization

AgriEngineering, Journal Year: 2024, Volume and Issue: 6(2), P. 1300 - 1318

Published: May 9, 2024

Hemp, commonly known as Cannabis sativa L., is a medicinal plant species of the Cannabaceae family. For efficient extraction C. leaves using conventional stirring process with water solvent, three crucial parameters (i.e., duration, liquid–solid ratio, and temperature) were investigated through response surface methodology (RSM). The concentrations extracted bioactive compounds (polyphenols, ascorbic acid, carotenoids) showed significant variations in RSM design points, suggesting importance finding optimal conditions which ratio temperature found to have highest impact. Further analysis was conducted on extract employing several assays determine their polyphenol content, total carotenoid color evaluation, anti-inflammatory activity, antioxidant capacity FRAP, DPPH, H2O2 assays. A low time (30 min) at 50 °C high (50:1) required for possible yield polyphenols. content determined be 9.76 mg gallic acid equivalents/g under optimum conditions, pelargonin being most abundant (1.51 mg/g) extracts. Ascorbic measured 282.23 μg/g carotenoids 356.98 μg/g. Correlation analyses revealed that activity negatively correlated specific As by DPPH (27.43 μmol equivalents (AAE)/g), FRAP (49.79 AAE/g), (230.95 AAE/g) assays, optimized aqueous capacity. Furthermore, it demonstrated considerable 17.89%, potential increase 75.12% particular conditions. Given added-value extracts, results this study highlight utility source health-improving pharmaceutical food industries.

Language: Английский

---

Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle ( Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS)

International Journal of Food Properties, Journal Year: 2023, Volume and Issue: 26(1), P. 2171 - 2185

Published: Aug. 8, 2023

The bioactive constituents of Nettle (Urtica dioica L.) plants were extracted by green and environmentally friendly methods using several deep eutectic solvents (DESs) followed liquid chromatography-mass spectrometry separation identification. To optimize the extraction process, bio-solvents composed organic acids such as hydrogen bond donors (HBD) mannitol/menthol/sugars HBA selected coupled with ultrasound-assisted extraction. maximum compound contents TPC 2423 mg GAE/100 g, TFC 134.71 CE/100 DPPH 1071.05 mol TE/mL. optimum parameters to achieve highest values phenolic compounds from via UAE-DES obtained at an temperature 70°C, time 30 min, water addition on DES 20%. ideal experimental conditions confirmed Central Composite Design (CCD) Response Surface Methodology (RSM) model analyze significance demonstrating high prediction levels compounds. Liquid chromatography diode array detection electrospray ionization tandem mass (LC-DAD-ESI-MS/MS) was used identify extracts. results showed that quercetin-3-O-rutinoside, 5-ocaffeoylquinic acid, caffeoyl malic acid three abundance. For first time, nettle a food-grade contains GRAS components is reported. According these findings, best solvent for extracting substances applications DESCAMAL (citric acid/maltose).

Language: Английский

---

Optimization of extraction of phenolic compounds and antioxidants from passion fruit and rambutan seeds using response surface methodology

Journal of Agriculture and Food Research, Journal Year: 2023, Volume and Issue: 14, P. 100888 - 100888

Published: Nov. 22, 2023

Passion fruit seed (PS) and rambutan (RS) contain sizable amounts of phenolic compounds (PCs) with bioactive including antioxidant activity (AOA). The aim this study was to optimize the extraction PCs from PS RS using response surface methodology (RSM). Preliminary results indicated that solid liquid (S-L) ratio 1:40 (w/v) showed highest yield (EY) for RS, values 11.59 ± 0.08 14.55 1.67 g/100 g samples, respectively. S-L 1:30 selected as an economic logistic. predicted compared experimental data EY, EPC, DPPH, ABTS FRAP extracts were 12.41 % (12.68 %), 715 (707), 85 (81), 539 (522) 107 (160) mg GAE/100 dry weight 13.68 (14.41 3141 (3235), 1327 (1434), 5793 (5564) 1567 (1509) PS, data, AOA, fitted a 2nd order polynomial equation. statistical analysis revealed ethanol concentration temperature significantly influenced (p < 0.001), whereas primarily affected on EY EPC. multiple regression suggested AOA by linear, quadratic, interaction concentration, time temperature. Nonetheless, only concentration. best possible combinations 67 54 %, 94 63 °C, 186 221 min, respectively, obtain maximum extractable (EPC) AOA. composite desirability optimal conditions 0.74 0.68, Optimal aqueous could serve sustainable food-grade ingredients nutraceutical products.

Language: Английский

---

Optimization of experimental conditions for bioactive compounds recovery from raspberry fruits (Rubus idaeus L.) by using combinations of ultrasound-assisted extraction and deep eutectic solvents

Applied Food Research, Journal Year: 2023, Volume and Issue: 3(2), P. 100346 - 100346

Published: Oct. 6, 2023

Raspberry fruits (Rubus idaeus L.) have been reported to contain phenolic compounds, of which anthocyanins are the most abundant. A novel procedure ultrasound-assisted extraction (UAE) using deep eutectic solvent (DES) for efficient compounds and from raspberries is considered. Namely, green approach optimization by response surface methodology (RSM) based on Box-Behnken Design (BBD) was proposed in this study. Three carboxylic acid-based DES, like acetic acid/glucose (DESAAGLU), formic (DESFAGLU), lactic acid/maltose (DESLAMA), were tested their efficiency recovery raspberries. The optimum parameters obtained; time 60 min, a volume 35 mL, added water DES 30 mL (v/v). results study indicated that optimizing conditions critical quantifying anthocyanins. present model can contribute finding cheap way delivering natural bioactive food, cosmetic, pharmaceutical industriesas well as food colorant dietary applications with potential health benefits.

Language: Английский

---

Transcriptome and biochemical analyses reveal phenolic compounds-mediated flavor differences in loquat (Eriobotrya japonica Lindl.) cultivars Chunhua No.1 and Dawuxing

Food Chemistry X, Journal Year: 2024, Volume and Issue: 21, P. 101145 - 101145

Published: Jan. 19, 2024

The novel loquat cultivar 'Chunhua No.1' (CH1) is a promising commercial cultivar. However, CH1 has texture characteristics different from those of common loquat, and its formation mechanism remains unclear. Here, we first identified the phenolic compounds parent ('Dawuxing', DWX) effect on formation. special presence stone cells explained flavor differences in CH1. Chlorogenic acid, neochlorogenic coniferyl alcohol were main high content was potential factor for rough Transcriptome reveals that phenylpropanoid metabolism activated during fruit Kyoto Encyclopedia Genes Genomes (KEGG) 51 structural genes involved biosynthesis, Weighted Gene Co-expression Network Analysis (WGCNA) four 88 transcription factors. These findings provide new insights into fruit.

Language: Английский

---

Bioactive Recovery from Watermelon Rind Waste Using Ultrasound-Assisted Extraction

ACS Food Science & Technology, Journal Year: 2024, Volume and Issue: 4(3), P. 687 - 699

Published: Feb. 13, 2024

In response to the imperative address food and agricultural waste promote environmental sustainability, this study investigates transformation of watermelon rind into a valuable resource. We extract polyphenols antioxidants from freeze-dried across four cultivars (Crimson Sweet, Black Prince, Chairman, Sugar Baby) using ultrasound-assisted extraction. Solvent selection, based on preliminary experiment, was optimized through surface methodology (RSM), fine-tuning solvent concentration sonication time. The RSM effectively modeled our responses (R2 > 0.90), confirming its suitability for optimization. Applying parameters (80% 20 min sonication) rinds yielded extracts rich in flavanols (10–30 mg/g) phenolics (1–2.5 mg/g), with high DPPH (22–40 mg Trolox/g) FRAP (26–42 ferrous sulfate/g) antioxidant activities. Furthermore, fatty acid phytosterol profiles were reported, demonstrating their potential applications food, pharmaceuticals, nutraceuticals, industry.

Language: Английский

---

Maximizing Bioactive Compound Extraction from Mandarin (Citrus reticulata) Peels through Green Pretreatment Techniques

Oxygen, Journal Year: 2024, Volume and Issue: 4(3), P. 307 - 324

Published: Aug. 11, 2024

This study explored the use of mandarin peels as an important source health-promoting compounds by utilizing green methods (i.e., pulsed electric field and ultrasound-assisted extraction), along with conventional stirring. The impact several extraction parameters, such duration, temperature, solvent composition, on recovery bioactive was evaluated through a response surface methodology. To identify most effective conditions for all assays, partial least-squares analysis implemented. It revealed that combination above techniques optimal at 80 °C 30 min, 75% v/v ethanol in water solvent. concentration optimum extract had total polyphenol content 18.69 mg gallic acid equivalents (GAE) per gram dry weight (dw), ascorbic 18.25 mg/g dw. However, correlation analyses rather negative relationship between these compounds. chromatographic extracts supported this result quantifying 20.53 dw individual polyphenols, hesperidin being dominant compound (13.98 dw). antioxidant including ferric-reducing power DPPH• inhibition activity, were measured 123.21 65.12 μmol (AAE) dw, respectively. research enhances valorization renewable compounds, providing opportunity to generate high-added-value products from food waste pharmaceutical sectors.

Language: Английский

---

Improvement of antioxidant capacity and gray mold resistance of strawberries by combined plasma technology

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 223, P. 113426 - 113426

Published: Feb. 4, 2025

Language: Английский

---