Unveiling the functional diversity of ionotropic glutamate receptors in the Pacific oyster (Crassostrea gigas) by systematic studies DOI Creative Commons
Xueshu Zhang, Linfang Zhang,

Yiran Si

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 25, 2023

Ionotropic glutamate receptors (iGluRs), pivotal in mediating excitatory neurosignals within the central nervous system, are instrumental environmental stress responses. In this investigation, 12 iGluRs identified Pacific oyster herein designated as Cg iGluRs, and further categorized into three distinct subfamilies based on their transmembrane domains. Cross-species evolutionary analysis unveiled a high degree of conservation sequence structural attributes these iGluRs. These ubiquitously distributed across various tissues, with pronounced expression oyster’s mantle, labial palps, gills, underlining integral role sensing mechanisms. Post D-shaped larval stage, marked upward trend was observed, denoting critical involvement development beyond phase. Exposure to five metals—cadmium (Cd), copper (Cu), zinc (Zn), mercury (Hg), lead (Pb)—elicited significant upregulation GRIA4 expression, indicating robust response metal stress. A KEGG enrichment 142 genes, exhibiting parallel trends under stress, suggests that could augment signal transmission by activating glutamatergic dopaminergic synapses, thereby contributing oyster. This inquiry not only bolsters our comprehension gene family but also paves way for future exploration its cardinal cellular signaling adaptability.

Language: Английский

Sufentanil-induced Nrf2 protein ameliorates cerebral ischemia-reperfusion injury through suppressing neural ferroptosis DOI
Xuelian Zhu,

Xi Han,

Jingtao Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135109 - 135109

Published: Aug. 27, 2024

Language: Английский

Citations

7
Establishment of liquid–liquid phase separation-related prognostic model in lung adenocarcinoma and systematic analysis of its clinical significance DOI Creative Commons

Y.-C. Chen,

Cheng Huang, Wei Wei

et al.

The International Journal of Biological Markers, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Purpose To detect the prognostic importance of liquid–liquid phase separation (LLPS) in lung adenocarcinoma. Methods The gene expression files, copy number variation data, and clinical data were downloaded from Cancer Genome Atlas cohort. LLPS-related genes acquired DrLLPS website. model based on LLPS was constructed by Cox regression LASSO analyses after identification differentially expressed (DEGs). Gene Ontology functional Kyoto Encyclopedia Genes Genomes enrichment analysis performed. risk score validated GSE31210 GSE72094. overall survival adenocarcinoma patients predicted plotting a nomogram. biological features high-risk evaluated CIBERSORT, ESTIMATE, Set Variation Analysis, Genomics Drug Sensitivity Cancer. Reverse transcription-quantitative polymerase chain reaction detected hub expression. Results A total 91 DEGs screened out LLPS, among which 9 discovered as biomarkers GRIA1, CRTAC1, MAGEA4, MAPK4 identified analysis. High-risk low-risk groups divided according to index, with group displaying markedly worse outcome. CRTAC1 significantly decreased, MAGEA4 expressions increased, while GRIA1 altered cells. Tumor microenvironment, signaling pathway enrichment, drug sensitivity differed between different groups. Conclusions This work proposed tool signature offer prospective effective for prognosis.

Language: Английский

Citations

0
Ischemic Tolerance—A Way to Reduce the Extent of Ischemia–Reperfusion Damage DOI Creative Commons
Rastislav Burda, Jozef Bürda, Radoslav Morochovič

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(6), P. 884 - 884

Published: March 13, 2023

Individual tissues have significantly different resistance to ischemia-reperfusion damage. There is still no adequate treatment for the consequences of By utilizing ischemic tolerance, it possible achieve a significant reduction in extent cell damage due injury. Since usually occurs unexpectedly, use preconditioning extremely limited. In contrast, postconditioning has wider possibilities practice. both cases, activation tolerance can also be achieved by application sublethal stress on remote organ. Despite very encouraging and successful results animal experiments, clinical been disappointing so far. To avoid factors that prevent solution blood plasma containing effectors. This taken from healthy donors which, after exposure two stresses within 48 h, effectors occur plasma. Application this activated recipient animals end lethal ischemia prevents death reduces Until there clear chemical identification products simplest way enhancing will preparation young with possibility its immediate recipients during initial treatment.

Language: Английский

Citations

8
The mechanism of hypoxia-inducible factor-1α enhancing the transcriptional activity of transferrin ferroportin 1 and regulating the Nrf2/HO-1 pathway in ferroptosis after cerebral ischemic injury DOI

Haiqian Yao,

Jianan Tian, Shi Cheng

et al.

Neuroscience, Journal Year: 2024, Volume and Issue: 559, P. 26 - 38

Published: Aug. 22, 2024

Language: Английский

Citations

2
Sevoflurane blocks KLF5‐mediated transcriptional activation of ITGB2 to inhibit macrophage infiltration in hepatic ischemia/reperfusion injury DOI
Ye Li,

Weinian Gao,

Shuyan Lei

et al.

The Journal of Gene Medicine, Journal Year: 2024, Volume and Issue: 26(5)

Published: May 1, 2024

Abstract Background Sevoflurane (Sevo) preconditioning and postconditioning play a protective role against injury induced by hepatic ischemia/reperfusion (I/R). At the same time, involvement of macrophage infiltration in this process precise mechanisms are unclear. Here, we designed research to elucidate effects Sevo I/R molecules involved. Methods The alleviating effect on liver was analyzed function analysis, hematoxylin eosin staining, Masson trichrome terminal deoxynucleotidyl transferase‐mediated 2′‐deoxyuridine 5′‐triphosphate nick end labeling, western blot analysis an enzyme‐linked immunosorbent assay. An vitro cell model developed using alpha mouse 12 (AML12) cells, treated with oxygen–glucose deprivation reoxygenation Sevo. Multiple bioinformatics databases were used screen transcriptional regulators related targets Krueppel‐like factor 5 (KLF5). KLF5 expression artificially upregulated alone or integrin beta‐2 (ITGB2) knockdown substantiate their Sevo‐mediated hepatoprotection. Results protected reducing apoptosis inflammatory response. tissues following injury, whereas overexpression aggravated injury. bound promoter ITGB2 enhance transcription. Knockdown reversed aggravation caused mice AML12 cells. Conclusions blocked KLF5‐mediated activation ITGB2, thereby inhibiting

Language: Английский

Citations

1
Unveiling the functional diversity of ionotropic glutamate receptors in the Pacific oyster (Crassostrea gigas) by systematic studies DOI Creative Commons
Xueshu Zhang, Linfang Zhang,

Yiran Si

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 25, 2023

Ionotropic glutamate receptors (iGluRs), pivotal in mediating excitatory neurosignals within the central nervous system, are instrumental environmental stress responses. In this investigation, 12 iGluRs identified Pacific oyster herein designated as Cg iGluRs, and further categorized into three distinct subfamilies based on their transmembrane domains. Cross-species evolutionary analysis unveiled a high degree of conservation sequence structural attributes these iGluRs. These ubiquitously distributed across various tissues, with pronounced expression oyster’s mantle, labial palps, gills, underlining integral role sensing mechanisms. Post D-shaped larval stage, marked upward trend was observed, denoting critical involvement development beyond phase. Exposure to five metals—cadmium (Cd), copper (Cu), zinc (Zn), mercury (Hg), lead (Pb)—elicited significant upregulation GRIA4 expression, indicating robust response metal stress. A KEGG enrichment 142 genes, exhibiting parallel trends under stress, suggests that could augment signal transmission by activating glutamatergic dopaminergic synapses, thereby contributing oyster. This inquiry not only bolsters our comprehension gene family but also paves way for future exploration its cardinal cellular signaling adaptability.

Language: Английский

Citations

2