The causal relationship between gut microbiota and biliary tract cancer: comprehensive bidirectional Mendelian randomization analysis

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 15, 2024

Background Growing evidence has shown that gut microbiome composition is associated with Biliary tract cancer (BTC), but the causality remains unknown. This study aimed to explore causal relationship between microbiota and BTC, conduct an appraisal of microbiome’s utility in facilitating early diagnosis BTC. Methods We acquired summary data for Genome-wide Association Studies (GWAS) pertaining BTC (418 cases 159,201 controls) from Biobank Japan (BBJ) database. Additionally, GWAS relevant (N = 18,340) were sourced MiBioGen consortium. The primary methodology employed analysis consisted Inverse Variance Weighting (IVW). Evaluations sensitivity carried out through utilization multiple statistical techniques, encompassing Cochrane’s Q test, MR-Egger intercept evaluation, global test MR-PRESSO, a leave-one-out methodological analysis. Ultimately, reverse Mendelian Randomization was conducted assess potential reciprocal causality. Results outcomes derived IVW substantiated presence Family Streptococcaceae (OR 0.44, P 0.034), Veillonellaceae 0.46, 0.018), Genus Dorea 0.29, 0.041) exerted protective influence against Conversely, Class Lentisphaeria 2.21, 0.017), Lachnospiraceae FCS020 Group 2.30, 0.013), Order Victivallales 0.017) adverse impact. To any effect, we used as exposure outcome, this revealed associations five different types microbiota. disclosed absence empirical indicators either heterogeneity or pleiotropy. Conclusion investigation represents inaugural identification indicative supporting beneficial detrimental relationships risk determined MR methodologies. These could hold significance formulation individualized therapeutic strategies at prevention survival enhancement.

Language: Английский

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Causal relationships between gut microbiota and male reproductive inflammation and infertility: Insights from Mendelian randomization

Medicine, Journal Year: 2025, Volume and Issue: 104(17), P. e42323 - e42323

Published: April 25, 2025

The study observed interactions between gut microbiota and male reproductive health, noting that the causal relationships were previously unclear. It aimed to explore potential cause-and-effect relationship bacteria problems such as inflammation, infertility, sperm functionality, using a two-sample Mendelian randomization method examine these connections. analysis found certain bacterial genera, Erysipelatoclostridium (0.71 [0.55-0.92]), Parasutterella (0.74 [0.57-0.96]), Ruminococcaceae UCG-009 (0.77 [0.60-0.98]), Slackia (0.69 [0.49-0.96]), showed protective effects against prostatitis. In contrast, other genera like Faecalibacterium (1.59 [1.08-2.34]), Lachnospiraceae UCG004 (1.64 [1.15-2.34]), Odoribacter (1.68 [1.01-2.81]), Paraprevotella (1.28 [1.03-1.60]), Sutterella (1.58 [1.13-2.19]) detrimental. Additionally, identified 2 orchitis epididymitis, 3 5 abnormal spermatozoa. Further of sperm-related proteins revealed associations specific SPACA3, SPACA7, SPAG11A, SPAG11B, SPATA9, SPATA20, ZPBP4. results remained robust after sensitivity reverse analysis. concluded have roles in sperm-associated proteins. This provides novel strategy for early diagnosis identification therapeutic targets inflammation infertility.

Language: Английский

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The predictive, preventive, and personalized medicine of insomnia: gut microbiota and inflammation

The EPMA Journal, Journal Year: 2023, Volume and Issue: 14(4), P. 571 - 583

Published: Nov. 17, 2023

Language: Английский

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Unraveling the relationship between gut microbiota and site-specific endometriosis: a Mendelian randomization analysis

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 4, 2024

Background Although numerous studies have illustrated the connection between gut microbiota and endometriosis, a conspicuous gap exists in research focusing on pathogenesis of endometriosis at various sites its linkage with infertility. Methods In this study, we used two-sample Mendelian randomization analysis to investigate effect development different regions, including uterus, ovary, fallopian tube, pelvic peritoneum, vagina, rectovaginal septum, as well intestine. Additionally, explored correlation endometriosis-induced Genetic associations microbes were obtained from genome-wide association study (GWAS) datasets provided by MiBioGen consortium, whereas endometriosis-related GWAS data sourced FinnGen dataset. our analysis, single-nucleotide polymorphisms instrumental variables, primary estimation causal performed via inverse variance weighting method. Our sensitivity analyses incorporated heterogeneity tests, pleiotropy leave-one-out Results We identified genus level four bacterial communities endometriosis. Subsequently, several subtypes anatomical recognized. Specifically, three genera linked ovarian six associated tubal showed links peritoneum five connected vaginal septum seven demonstrated linkages intestinal one was adenomyosis, exhibited Conclusion elucidates site-specific thereby augmenting understanding pathophysiology Moreover, findings pave way for potential therapeutic strategies targeting individuals grappling

Language: Английский

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The predictive, preventive, and personalized medicine of depression: gut microbiota and inflammation

The EPMA Journal, Journal Year: 2024, Volume and Issue: 15(4), P. 587 - 598

Published: Sept. 20, 2024

Language: Английский

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Genetically predicted the causal relationship between gut microbiota and the risk of polymyositis/dermatomyositis: a Mendelian randomization analysis

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 21, 2024

Introduction Observational studies suggest associations between gut microbiota and polymyositis (PM) dermatomyositis (DM), but causal relationships are unclear. We investigate the effects of on PM DM, providing insights hoping to provide for future treatment prevention. Methods Summary statistics were obtained from a multi-ethnic Genome Wide Association Studies (GWAS) meta-analysis, including 119 taxa 18,340 Europeans. PM/DM extracted GWAS analyses. Mendelian randomization (MR) with IVW, MR-Egger, weighted median methods was performed. Sensitivity analyses addressed heterogeneity pleiotropy. Of bacterial genera studied, six showed links. Results Alloprevotella (OR: 3.075, 95% CI: 1.127–8.386, p = 0.028), Ruminococcaceae UCG003 4.219, 1.227–14.511, 0.022), Dialister 0.273, 0.077–0.974, 0.045) associated PM. Anaerotruncus 0.314, 0.112–0.882, UCG002 2.439, 1.173–5.071, 0.017), Sutterella 3.392, 1.302–8.839, 0.012) related DM. validated these Discussion establish , UCG003, Common microbiota, like have significant clinical implications. These findings open up greater possibilities contribute development monitoring in patients PM/DM.

Language: Английский

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Causal Link between Gut Microbiota and Infertility: A Two-sample Bidirectional Mendelian Randomization Study

Current Medical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Language: Английский

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Causal relationship between gut microbiota and myasthenia gravis: a two-sample Mendelian randomization study

Frontiers in Neurology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 25, 2024

Background Previous observational studies have provided cumulative data linking gut microbiota to myasthenia gravis (MG). However, the causal link between two remains unexplored. Hence, current study was performed explore them. Methods Mendelian randomization (MR) analysis conducted using summary statistics of 211 taxa and largest genome-wide association (GWAS) for MG currently available. The inverse variance-weighted (IVW), MR-Egger, weighted median, mode methods were employed ascertain influence. Sensitivity utilizing several methodologies then used assess robustness findings. Lastly, evaluate reverse causality, a MR performed. Results Seven suggestive associations gastrointestinal identified based on outcomes analysis. Specifically, phylum Actinobacteria (OR: 0.602, 95% CI: 0.405–0.896, p = 0.012), class Gammaproteobacteria 0.587, 0.357–0.968, 0.037), families Defluviitaleaceae 0.695, 0.485–0.996, 0.047), Family XIII 0.614, 0.412–0.916, 0.017), Peptococcaceae 0.698, 0.505–0.964, 0.029) had protective effects MG, while order Mollicutes RF9 1.424, 1.015–1.998, 0.041) genus Faecalibacterium 1.763, 1.220–2.547, 0.003) risk factors MG. indicate that neither heterogeneity nor horizontal pleiotropy any discernible impact. Nevertheless, this did not reveal apparent effect composition. Conclusion investigation has substantiated connection which may provide helpful insights innovative therapeutic preventative approaches Further randomized controlled trials are needed elucidate microbiota’s precise role potential in pathogenesis

Language: Английский

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Causal Effects of Gut Microbiota on Female Reproductive Tract Inflammation and Infertility: A Mendelian Randomization Study

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 13, 2023

Abstract Background Studies indicate a potential connection between gut microbiota and reproductive health issues in women, such as inflammation infertility. However, the exact relationship remains uncertain, highlighting need for further research. Results A two-sample Mendelian Randomization (MR) analysis was conducted to investigate pelvic inflammatory diseases The study identified that specific microbiota, Lachnospiraceae UCG001 Ruminococcus 2, increased risk of disorders female organs (OR [95%CI]: 1.13 [1.01–1.26] 1.16 [1.03–13.1], respectively). In contrast, Butyricicoccus Prevotella 7 were associated with reduced risk. Further validation another dataset confirmed Butyricicoccus's role decreasing disease also found causal organs, including salpingitis oophoritis, uterine inflammation, cervical vulvar or vaginal inflammation. Regarding infertility, Faecalibacterium linked an (1.31 [1.08–1.60]), while genera like Erysipelotrichaceae UCG003 (torques group) lower risks. analyses showed various types anovulation, tubal factors, others. These results by sensitivity analysis, no reverse causation found. Conclusions This provides compelling evidence health. Identification bacterial decreased offers new possibilities early diagnosis targeted treatment.

Language: Английский

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Application of Mendelian randomization to assess host gene–gut microbiota correlations in patients with esophageal cancer

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 21, 2023

Background Increasing evidence suggests that esophageal cancer (ESCA) may be correlated with gut flora. However, their causal connection remains unclear. This study aimed to evaluate potential linkages and gene–gut microbiome associations between the microbiota ESCA using Mendelian randomization (MR). Methods We analyzed data genome-wide association studies. The exposure factor outcome variable were ESCA, respectively. MR-Egger method, weighted median, inverse-variance heterogeneity test, sensitivity analysis, multiplicity analysis used for MR analysis. And it was validated an external dataset. Further meta-analysis performed validate robustness of this relationship. Finally, we annotated single nucleotide polymorphisms in causally associated explore possible host gene-gut correlations patients ESCA. Results identified four species Three these had a negative relationship (odds ratio (OR): 0.961; 95% confidence interval (CI): 0.923–0.971; p = 0.047 Romboutsia ; OR: 0.972; CI: 0.921–0.961; 0.018 Lachnospira 0.948; 0.912–0.970; 0.032 Eubacterium ). A positive observed one bacterial group (OR: 1.105; 1.010–1.072; Veillonella External datasets show same trend. is further supported by meta-analysis. None showed pleiotropy, leave-one-out indicated reliability findings. microbiomes correlate 19 genes. Conclusion Our indicate link bacteria identify correlation genes offering novel therapeutic options.

Language: Английский

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