CRISPR/Cas12a-based method coupled with isothermal amplification to identify Alternaria spp. isolated from wheat grain samples DOI Creative Commons
Aisha Shaizadinova, Meruyert Amanzholova, И.В. Рукавицина

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 15, 2025

Alternaria fungal species are considered major plant pathogens, infecting various crops and resulting in significant agricultural losses. Additionally, these can contaminate grain with multiple mycotoxins that harmful to humans animals. Efficient pest management relies on timely detection identification of phytopathogens samples, facilitating prompt selection a crop protection strategy. Conventional tools, such as morphological characterization based polymerase chain reaction (PCR)-based methods, time-consuming laboratory-bound, limiting their implementation for on-site diagnostics essential the industry. Isothermal amplification including nucleic acid sequence-based (NASBA), loop-mediated isothermal (LAMP), recombinase (RPA), enable at constant temperatures, making them ideal point-of-care without need thermal cycling equipment. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (Cas12a)-based identification, coupled represents an emerging acid-based technology detecting pathogens high accuracy sensitivity. This study aimed develop CRISPR/Cas12a-based method integrated RPA specific spp. isolated from wheat samples. The developed targeted β-tubulin gene was successfully identified strains within 20-min followed by 30-min CRISPR/Cas12a visualization results. Specificity test included pathogenic commonly hosted grain, Fusarium Bipolaris sorokiniana, Nigrospora oryzae revealed specificity species. Furthermore, exhibited sensitivity, DNA down 100 copies, validated real-time fluorescence readout. A assay employed visualize results reaction, demonstrating substantial potential In conclusion, we present potentially sustainable approach rapid, precise, nucleic-acid-based

Language: Английский

Expanding antibiotic, vaccine, and diagnostics development and access to tackle antimicrobial resistance DOI
Ramanan Laxminarayan, Isabella Impalli, Radha Rangarajan

et al.

The Lancet, Journal Year: 2024, Volume and Issue: 403(10443), P. 2534 - 2550

Published: May 23, 2024

Language: Английский

Citations

49

Nanotechnology-leveraged nucleic acid amplification for foodborne pathogen detection DOI
Ruijie Deng, Jinrong Bai, Hao Yang

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 506, P. 215745 - 215745

Published: Feb. 21, 2024

Language: Английский

Citations

18

Surface Components and Biological Interactions of Extracellular Vesicles DOI
Jinping Wang, Kuoran Xing, Guo-Ying Zhang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.

Language: Английский

Citations

3

Recent advances in rapid detection of Helicobacter pylori by lateral flow assay DOI
Yuqing Dong, Zhu Jie, Naisui Pan

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(2)

Published: Jan. 16, 2025

Language: Английский

Citations

1

Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens DOI
Yanmei Feng,

Xinyu Zhao,

Qinghua Ye

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104882 - 104882

Published: Jan. 1, 2025

Language: Английский

Citations

1

Visualization Methods for Loop Mediated Isothermal Amplification (LAMP) Assays. DOI
Vinni Thekkudan Novi, Anil Kumar Meher, Abdennour Abbas

et al.

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Recent advances in nucleic acid (NA) detection techniques have significantly enhanced the diagnosis of diseases caused by a range pathogens. These NA-based methods that target specific gene sequences for identification offer high specificity. Despite effectiveness polymerase chain reaction (PCR), its requirement sophisticated laboratory settings and expensive equipment restricts accessibility, particularly resource-limited settings. As an alternative, isothermal amplification are highly sought after due to their rapid, sensitive, ability. Among these, loop mediated (LAMP) stands out simplicity, reliability, cost-effectiveness. LAMP operates without need varied temperature cycles, employing simple heating block maintain constant temperature, thus facilitating onsite rapid testing. In LAMP, step is critical as it shows outcome assay. order make technique user-friendly applicable large scale testing, visual where results can be observed with naked eye. This review focuses on recent developments visualization techniques, including more common fluorescence, turbidity, gel electrophoresis methods, well innovations colorimetric applying novel transduction such nanoparticles digital tools. Additionally, various practical applications discussed.

Language: Английский

Citations

1

Mechanism of DNA multimerization caused by strand-displacement DNA polymerases DOI
Assol R. Sakhabutdinova, Р.Р. Гарафутдинов

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: 703, P. 115876 - 115876

Published: April 19, 2025

Language: Английский

Citations

1

Tetrahedral DNA nanostructures enhance transcription isothermal amplification for multiplex detection of non-coding RNAs DOI

Eun Sung Lee,

Ji-Su Woo,

Jiye Shin

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 250, P. 116055 - 116055

Published: Jan. 19, 2024

Language: Английский

Citations

7

Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection DOI Creative Commons

Xiaolei Mao,

Minghui Xu,

Shuyin Luo

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Oct. 10, 2023

In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR. This paper explores fundamental principles recombinase polymerase (RPA) recombinase-aid (RAA) reviews current status integrating CRISPR-Cas system with RPA/RAA techniques. Furthermore, this confluence technology, providing comprehensive review enhancements existing combined methodologies such SHERLOCK DETECTR. We investigate practical applications in conjunction for highlighting how integrated approach significantly advances both research clinical implementation field. aims provide readers concise understanding fusion offering insights into their utility, ongoing enhancements, promising prospects detection.

Language: Английский

Citations

17

Practical diagnostic algorithms for Chagas disease: a focus on low resource settings DOI
Juan Carlos Gabaldón-Figueira, Malia Skjefte, Silvia A. Longhi

et al.

Expert Review of Anti-infective Therapy, Journal Year: 2023, Volume and Issue: 21(12), P. 1287 - 1299

Published: Nov. 7, 2023

Introduction Chagas disease, caused by parasite Trypanosoma cruzi, is the most important neglected tropical disease in Americas. Two drugs are available for treatment, but access to them challenging, part due complex diagnostic algorithms. These stage-dependent, involve multiple tests, and ill-adapted reality of vast areas where endemic. Molecular serologic tools used detect acute chronic infections, with performance latter showing geographic differences. Breakthroughs development new include validation a loop-mediated isothermal amplification assay infections (T. cruzi-LAMP), regional several rapid tests (RDTs) infection, which simplify testing resource-limited settings. The literature search was carried out MEDLINE database until 1 August 2023.

Language: Английский

Citations

14