Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 15, 2025
Alternaria
fungal
species
are
considered
major
plant
pathogens,
infecting
various
crops
and
resulting
in
significant
agricultural
losses.
Additionally,
these
can
contaminate
grain
with
multiple
mycotoxins
that
harmful
to
humans
animals.
Efficient
pest
management
relies
on
timely
detection
identification
of
phytopathogens
samples,
facilitating
prompt
selection
a
crop
protection
strategy.
Conventional
tools,
such
as
morphological
characterization
based
polymerase
chain
reaction
(PCR)-based
methods,
time-consuming
laboratory-bound,
limiting
their
implementation
for
on-site
diagnostics
essential
the
industry.
Isothermal
amplification
including
nucleic
acid
sequence-based
(NASBA),
loop-mediated
isothermal
(LAMP),
recombinase
(RPA),
enable
at
constant
temperatures,
making
them
ideal
point-of-care
without
need
thermal
cycling
equipment.
Clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
protein
12a
(Cas12a)-based
identification,
coupled
represents
an
emerging
acid-based
technology
detecting
pathogens
high
accuracy
sensitivity.
This
study
aimed
develop
CRISPR/Cas12a-based
method
integrated
RPA
specific
spp.
isolated
from
wheat
samples.
The
developed
targeted
β-tubulin
gene
was
successfully
identified
strains
within
20-min
followed
by
30-min
CRISPR/Cas12a
visualization
results.
Specificity
test
included
pathogenic
commonly
hosted
grain,
Fusarium
Bipolaris
sorokiniana,
Nigrospora
oryzae
revealed
specificity
species.
Furthermore,
exhibited
sensitivity,
DNA
down
100
copies,
validated
real-time
fluorescence
readout.
A
assay
employed
visualize
results
reaction,
demonstrating
substantial
potential
In
conclusion,
we
present
potentially
sustainable
approach
rapid,
precise,
nucleic-acid-based
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
Extracellular
vesicles
(EVs)
are
critical
mediators
of
intercellular
communication,
carrying
bioactive
cargo
and
displaying
diverse
surface
components
that
reflect
their
cellular
origins
functions.
The
EV
surface,
composed
proteins,
lipids,
glycocalyx
elements,
plays
a
pivotal
role
in
targeting
recipient
cells,
mediating
biological
interactions,
enabling
selective
delivery.
This
review
comprehensively
examined
the
molecular
architecture
surfaces,
linking
biogenesis
to
functional
diversity,
highlights
therapeutic
diagnostic
potential
diseases
such
as
cancer
cardiovascular
disorders.
Additionally,
we
explore
emerging
applications
EVs,
including
machine-learning-assisted
analysis,
chemical
integration,
cross-system
combinations.
also
discusses
some
key
challenges
clinical
translation
EV-related
technologies.
The Analyst,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Recent
advances
in
nucleic
acid
(NA)
detection
techniques
have
significantly
enhanced
the
diagnosis
of
diseases
caused
by
a
range
pathogens.
These
NA-based
methods
that
target
specific
gene
sequences
for
identification
offer
high
specificity.
Despite
effectiveness
polymerase
chain
reaction
(PCR),
its
requirement
sophisticated
laboratory
settings
and
expensive
equipment
restricts
accessibility,
particularly
resource-limited
settings.
As
an
alternative,
isothermal
amplification
are
highly
sought
after
due
to
their
rapid,
sensitive,
ability.
Among
these,
loop
mediated
(LAMP)
stands
out
simplicity,
reliability,
cost-effectiveness.
LAMP
operates
without
need
varied
temperature
cycles,
employing
simple
heating
block
maintain
constant
temperature,
thus
facilitating
onsite
rapid
testing.
In
LAMP,
step
is
critical
as
it
shows
outcome
assay.
order
make
technique
user-friendly
applicable
large
scale
testing,
visual
where
results
can
be
observed
with
naked
eye.
This
review
focuses
on
recent
developments
visualization
techniques,
including
more
common
fluorescence,
turbidity,
gel
electrophoresis
methods,
well
innovations
colorimetric
applying
novel
transduction
such
nanoparticles
digital
tools.
Additionally,
various
practical
applications
discussed.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: Oct. 10, 2023
In
the
realm
of
pathogen
detection,
isothermal
amplification
technology
has
emerged
as
a
swift,
precise,
and
sensitive
alternative
to
conventional
PCR.
This
paper
explores
fundamental
principles
recombinase
polymerase
(RPA)
recombinase-aid
(RAA)
reviews
current
status
integrating
CRISPR-Cas
system
with
RPA/RAA
techniques.
Furthermore,
this
confluence
technology,
providing
comprehensive
review
enhancements
existing
combined
methodologies
such
SHERLOCK
DETECTR.
We
investigate
practical
applications
in
conjunction
for
highlighting
how
integrated
approach
significantly
advances
both
research
clinical
implementation
field.
aims
provide
readers
concise
understanding
fusion
offering
insights
into
their
utility,
ongoing
enhancements,
promising
prospects
detection.
Expert Review of Anti-infective Therapy,
Journal Year:
2023,
Volume and Issue:
21(12), P. 1287 - 1299
Published: Nov. 7, 2023
Introduction
Chagas
disease,
caused
by
parasite
Trypanosoma
cruzi,
is
the
most
important
neglected
tropical
disease
in
Americas.
Two
drugs
are
available
for
treatment,
but
access
to
them
challenging,
part
due
complex
diagnostic
algorithms.
These
stage-dependent,
involve
multiple
tests,
and
ill-adapted
reality
of
vast
areas
where
endemic.
Molecular
serologic
tools
used
detect
acute
chronic
infections,
with
performance
latter
showing
geographic
differences.
Breakthroughs
development
new
include
validation
a
loop-mediated
isothermal
amplification
assay
infections
(T.
cruzi-LAMP),
regional
several
rapid
tests
(RDTs)
infection,
which
simplify
testing
resource-limited
settings.
The
literature
search
was
carried
out
MEDLINE
database
until
1
August
2023.
