On-chip fabrication of tailored 3D hydrogel scaffolds to model cancer cell invasion and interaction with endothelial cells DOI Creative Commons
Federico Cantoni, Laurent Barbe, Ananya Roy

et al.

APL Bioengineering, Journal Year: 2024, Volume and Issue: 8(4)

Published: Dec. 1, 2024

The high mortality associated with certain cancers can be attributed to the invasive nature of tumor cells. Yet, complexity studying invasion hinders our understanding how spreads. This work presents a microengineered three-dimensional (3D) in vitro model for cancer cell and interaction endothelial was generated by printing biomimetic hydrogel scaffold directly on chip using 2-photon polymerization that simulates brain's extracellular matrix. scaffold's geometry specifically designed facilitate growth continuous layer cells one side, while also allowing introduction other side. arrangement confines spatially enables situ microscopy as they invade interact layer. We examined impact 3D parameters hydrogel's physical properties used patient derived glioblastoma study their effect invasion. Notably, tended infiltrate faster when an barrier present. potential adjusting properties, coupled capability real-time observation tumor-endothelial interactions, offers platform tumor–endothelial interactions.

Language: Английский

Surface Modification Strategies for Biomedical Applications: Enhancing Cell–Biomaterial Interfaces and Biochip Performances DOI

Soonjong Roh,

Yerim Jang,

Jin Yoo

et al.

BioChip Journal, Journal Year: 2023, Volume and Issue: 17(2), P. 174 - 191

Published: June 1, 2023

Language: Английский

Citations

40
Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling DOI Creative Commons
Ungsig Nam, Seokhun Lee, Ashfaq Ahmad

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: 18(3), P. 345 - 356

Published: May 14, 2024

Abstract The vascular system, essential for human physiology, is vital transporting nutrients, oxygen, and waste. Since structures are involved in various disease pathogeneses exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific models. While animal models possess sophisticated morphologies, they significant discrepancies from tissues due species differences, which limits their applicability. To overcome limitations arising these oversimplification of 2D dish cultures, microphysiological systems (MPS) emerged as a promising alternative. These more accurately mimic microenvironment by incorporating cell interactions, physical stimuli, extracellular matrix components, thus facilitating enhanced tissue differentiation functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model patient responses. This review focuses recent advancements MPS, particularly modeling discusses potential biological adaptation.

Language: Английский

Citations

7
Biosensor-Enhanced Organ-on-a-Chip Models for Investigating Glioblastoma Tumor Microenvironment Dynamics DOI Creative Commons
Gayathree Thenuwara, Bilal Javed, Baljit Singh

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(9), P. 2865 - 2865

Published: April 30, 2024

Glioblastoma, an aggressive primary brain tumor, poses a significant challenge owing to its dynamic and intricate tumor microenvironment. This review investigates the innovative integration of biosensor-enhanced organ-on-a-chip (OOC) models as novel strategy for in-depth exploration glioblastoma microenvironment dynamics. In recent years, transformative approach incorporating biosensors into OOC platforms has enabled real-time monitoring analysis cellular behaviors within controlled Conventional in vitro vivo exhibit inherent limitations accurately replicating complex nature progression. addresses existing research gap by pioneering models, providing comprehensive platform investigating The applications this combined studying dynamics are critically scrutinized, emphasizing potential bridge between simplistic conditions. Furthermore, article discusses implications elucidating features microenvironment, encompassing cell migration, proliferation, interactions. By furnishing insights, these significantly contribute unraveling biology glioblastoma, thereby influencing development more accurate diagnostic therapeutic strategies.

Language: Английский

Citations

4
Passive-Flow-Based MPS: Emerging Physiological Flow-Mimetic Platforms for Studying Effects of Flow on Single Tissues and Inter-tissue Interactions DOI

Geonho Jin,

Dohyung Kim,

Seonghun Mun

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: 18(2), P. 186 - 210

Published: March 8, 2024

Language: Английский

Citations

3
Advancements in Kidney-on-Chip: Antibiotic-Induced Kidney Injury and Future Directions DOI
Minhyeok Kim, Yugyeong Lee,

Gwang Myeong Seo

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: unknown

Published: June 27, 2024

Language: Английский

Citations

3
Open Microfluidic Cell Culture in Hydrogels Enabled by 3D-Printed Molds DOI Creative Commons

MM O'Brien,

Ashley N. Spirrison,

Melati S. Abdul Halim

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(2), P. 102 - 102

Published: Jan. 22, 2025

Cell culture models with tissue-mimicking architecture enable thein vitro investigation of cellular behavior and cell–cell interactions. These can recapitulate the structure function physiological systems be leveraged to elucidate mechanisms disease. In this work, we developed a method create open microfluidic cell cultures in using 3D-printed molds. The improves sample accessibility, is simpler manufacture than traditional closed requires minimal specialized equipment, making it an attractive for applications. Further, these molds generate multiple structures various hydrogels, including blood vessel mimics endothelial cells (HUVECs). Various geometries were patterned into agarose, gelatin, collagen type I star-shaped wells, square round channels, demonstrate versatility approach. Open channels created diameters ranging from 400 µm 4 mm densities 2 mg/mL mg/mL. To applicability our approach tissue modeling, generated 800 mm, high viability (>89%) both dimensions. used study effects hypoxia on CD31 expression by subjecting them reduced-O2 environment (∼16% O2). As compared normoxia conditions, under had reduction 8.3% surface 7.4%. Overall, enables generation different hydrogels development biological

Language: Английский

Citations

0
Understanding Neovascularization in Glioblastoma: Insights from the Current Literature DOI Open Access
Mariagiovanna Ballato, Emanuela Germanà, Gabriele Ricciardi

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2763 - 2763

Published: March 19, 2025

Glioblastomas (GBMs), among the most aggressive and resilient brain tumors, characteristically exhibit high angiogenic potential, leading to formation of a dense yet aberrant vasculature, both morphologically functionally. With these premises, numerous expectations were initially placed on anti-angiogenic therapies, soon dashed by their limited efficacy in concretely improving patient outcomes. Neovascularization GBM emerged as complex, dynamic, heterogeneous process, hard manage with classical standard care. Growing evidence has revealed existence non-canonical strategies angiogenesis, variously exploited meet its ever-increasing metabolic demand differently involved tumor progression, recurrence, escape from treatments. In this review, we provide an accurate description each neovascularization mode encountered tumors date, highlighting molecular players signaling cascades primarily involved. We also detail key architectural functional aspects characteristic vascular compartment because intricate crosstalk between different networks. Additionally, explore repertoire emerging therapies against that are currently under study, concluding question: faced such challenging scenario, could combined tailored patient’s genetic signatures, represent effective game changer?

Language: Английский

Citations

0
3D vascularized microphysiological system for investigation of tumor-endothelial crosstalk in anti-cancer drug resistance DOI
Seunggyu Kim, Joonha Park, Jin‐Nyoung Ho

et al.

Biofabrication, Journal Year: 2023, Volume and Issue: 15(4), P. 045016 - 045016

Published: Aug. 11, 2023

Despite the advantages of microfluidic system in drug screening, vascular systems responsible for transport drugs and nutrients have been hardly considered microfluidic-based chemotherapeutic screening. Considering physiological characteristics highly vascularized urinary tumors, we here investigated response bladder tumor cells using a on chip. The chip was designed to open-top region sample introduction hydrophilic rail spontaneous hydrogel patterning, which contributed construction tumor-hydrogel-endothelium interfaces spatiotemporal on-demand manner. Utilizing where intravascularly injected cisplatin diffuse across endothelium into samples, responses cisplatin-resistant or -susceptible were evaluated, showing preservation cellular resistance even within structure also enabled direct harvest samples post analysis terms secretome gene expressions. Comparing efficacy presence absence endothelium, found that proliferation rates increased vasculature-incorporated These suggested our allows establishment vascular-gel-tumor manners further enables investigations

Language: Английский

Citations

8
Recent advances in micro-physiological systems for investigating tumor metastasis and organotropism DOI
Heejeong Yoon, Jonathan Sabaté del Río,

Seung Woo Cho

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1351 - 1366

Published: Jan. 1, 2024

MPS technology holds great potential for studying complex metastasis organotropism. It effectively simulates the dynamic interactions between distinct organ environments and tumor cells, capturing both their inter- intra-tumor heterogeneity.

Language: Английский

Citations

2
Monoamine Oxidase-A (MAO-A) Inhibitors Screened from the Autodisplayed Fv-Antibody Library DOI Open Access

Jeong Soo Sung,

Seung‐Whan Kim, Jaeyong Jung

et al.

ACS Pharmacology & Translational Science, Journal Year: 2023, Volume and Issue: 7(1), P. 150 - 160

Published: Dec. 26, 2023

Serotonin-like mimotopes were screened from the Fv-antibody library to be used as inhibitors against monoamine oxidase A (MAO-A). The [corresponding VH region of immunoglobulin G (IgG)] consists three complementarity-determining regions and four frame regions. was prepared by site-directed mutagenesis CDR3, which 11 amino acid residues. Three target clones library, binding affinity monoclonal anti-serotonin antibody analyzed using fluorescence-activated cell sorting. Fv-antibodies expressed soluble proteins fused with green fluorescence protein. Additionally, CDR3 (11 residues) selected synthesized peptides linking constants (KD) serotonin-like (Fv-antibodies peptides) estimated a surface plasmon resonance biosensor. inhibitory activity (IC50) separately for MAO-A MAO-B enzymes compared that conventional inhibitors. Finally, treat line (SH-SY5Y, ATCC code: CRL-2266) expressing serotonin receptors. This done confirm following two aspects: (1) receptors on (2) in lysates.

Language: Английский

Citations

6