Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: Jan. 21, 2025

Abstract Background Uterine Corpus Endometrial Carcinoma (UCEC) is a prevalent gynecologic malignancy with complex molecular underpinnings. This study identifies key woundhealing genes involved in UCEC and elucidates their roles through comprehensive analysis. Methods In silico vitro experiments. Results Seventy wound healing-associated were extracted from the Gene Ontology (GO) database, protein-protein interaction (PPI) network was constructed using STRING database. CytoHubba analysis Cytoscape identified six pivotal hub genes: CD44, FGF2, FGF10, KDM6A, FN1, MMP2. These exhibited significantly lower expression cell lines compared to normal controls, as confirmed by RT-qPCR. Receiver Operating Characteristic (ROC) demonstrated potential diagnostic biomarkers, Area Under Curve (AUC) values ranging 0.94 1.00. Validation TCGA datasets revealed consistent downregulation of these samples, corroborated immunohistochemical staining. Promoter methylation showed higher levels UCEC, correlating decreased mRNA poor survival outcomes. Genetic alteration indicated frequent mutations FN1 although did not affect survival. Functional CancerSEA database highlighted involvement critical cancer-related processes, including angiogenesis, apoptosis, metastasis. Immune correlation studies significant associations immune inhibitor distinct patterns across subtypes. Overexpression that CD44 MMP2 reduce proliferative ability while enhancing migration healing. Conclusion Collectively, findings underscore crucial pathogenesis, highlighting biomarkers therapeutic targets this malignancy.

Language: Английский

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Mesenchymal stem cell transplantation in burn wound healing: uncovering the mechanisms of local regeneration and tissue repair

Histochemistry and Cell Biology, Journal Year: 2023, Volume and Issue: 161(2), P. 165 - 181

Published: Oct. 17, 2023

Abstract Burn injuries pose a significant healthcare burden worldwide, often leading to long-term disabilities and reduced quality of life. To explore the impacts transplantation mesenchymal stem cells (MSCs) on healing burns levels serum cytokines, 60 fully grown Sprague–Dawley rats were randomly divided into three groups ( n = 20 each): group I (control), II (burn induction), III induction + bone marrow (BM)-MSC transplantation). Groups further four subgroups 5 each) based euthanasia duration (7, 14, 21, 28 days post transplant). The experiment concluded with an anesthesia overdose for rat death. After 7, days, assessed by clinical, laboratory, histopathology investigations. results revealed improvements in burn potentiality treated MSC. Furthermore, cytokine measured, increases interleukin (IL)-6 interferon alpha (IFN) observed, while IL-10 transforming growth factor beta (TGF-β) decreased at 7 increased until burn. Also, that underwent exhibited pro-inflammatory cytokines anti-inflammatory when compared control group. Histological assessments showed better re-epithelialization, neovascularization, collagen deposition experimental group, suggesting MSC wounds may promote modulating immune response promoting tissue regeneration.

Language: Английский

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Efficient healing of diabetic wounds by MSC-EV-7A composite hydrogel via suppression of inflammation and enhancement of angiogenesis

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(7), P. 1750 - 1760

Published: Jan. 1, 2024

An alginate hydrogel loaded with mesenchymal stem-cell-derived extracellular vesicles (EVs) and a 7-amino-acid peptide (7A) constitutes novel dressing for efficient healing of diabetic wounds anti-inflammation pro-angiogenesis mechanisms.

Language: Английский

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Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Materials Today Bio, Journal Year: 2023, Volume and Issue: 20, P. 100686 - 100686

Published: June 1, 2023

Umbilical cord-derived mesenchymal stem cells (UC-MSC) are promising candidates for wound healing. However, the low amplification efficiency of MSC in vitro and their survival rates after transplantation have limited medical application. In this study, we fabricated a micronized amniotic membrane (mAM) as microcarrier to amplify used mAM (mAM-MSC) complexes repair burn wounds. Results showed that could live proliferate on 3D culture system, exhibiting higher cell activity than 2D culture. Transcriptome sequencing expression growth factor-related, angiogenesis-related, healing-related genes was significantly upregulated mAM-MSC compared traditional 2D-cultured MSC, which verified via RT-qPCR. Gene ontology (GO) analysis differentially expressed (DEGs) significant enrichment terms related proliferation, angiogenesis, cytokine activity, healing mAM-MSC. model C57BL/6J mice, topical application accelerated injection alone accompanied by longer greater neovascularization wound.

Language: Английский

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Extracellular Vesicles in Atherosclerosis: State of the Art

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 388 - 388

Published: Dec. 27, 2023

Atherosclerosis is a chronic inflammatory disease driven by lipid accumulation in the arteries, leading to narrowing and thrombosis that causes mortality. Emerging evidence has confirmed atherosclerosis affects younger people involved majority of deaths worldwide. EVs are associated with critical steps atherosclerosis, cholesterol metabolism, immune response, endothelial dysfunction, vascular inflammation, remodeling. Endothelial cell-derived can interact platelets monocytes, thereby influencing atherosclerotic plaque destabilization, formation thrombus. potential diagnostic prognostic biomarkers (AS) cardiovascular (CVD). Importantly, derived from stem/progenitor cells essential mediators cardiogenesis cardioprotection may be used regenerative medicine tissue engineering.

Language: Английский

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Agarose Microgel-Based In Situ Cleavable Immuno-Rolling Circle Amplification for Multiplexed Single-Molecule Quantitation on Single Extracellular Vesicles

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 4, 2025

We have developed a platform for the multiplexed and ultrasensitive profiling of individual extracellular vesicles (EVs) directly in plasma, which we call GDEVA─Agarose microGel-based Digital single-molecule-single EV Assay. GDEVA achieves single-molecule sensitivity moderate multiplexing (demonstrated 3-plex), can achieve throughput ∼104 EVs per minute necessary to resolve human plasma when read out using flow cytometry. Our integrates rolling circle amplification (RCA) immunoassay surface proteins, are cleaved from single EVs, amplified within agarose microgels, followed by cytometry-based readout or imaging after fluorescence-activated cell sorting (FACS). It overcomes steric hindrance RCA products, nonspecific binding templates, lack quantitation multiple proteins on that plagued earlier approaches. evaluated analytical capabilities through head-to-head comparison with conventional technology demonstrated ∼100× improvement limit detection (LOD) subpopulations. evaluate GDEVA's potential cancer immunology, analyzing samples patients melanoma, where heterogeneity plays critical role disease progression response therapy. demonstrate key immune markers PD-L1, CD155, melanoma marker TYRP-1, showed precisely quantify offering resolution detect rare subpopulations complex clinical specimens.

Language: Английский

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AN OVERVIEW OF NEW TRENDS IN THE COSMETICS INDUSTRY

International Journal of Applied Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 136 - 147

Published: May 7, 2025

Cosmetic business is one of the major billers global economy since it serves a market which growing up continuously due to different reasons such as consumer behavior changes, technological and scientific developments, in addition rise awareness. This review focuses on giving an idea about importance cosmetics industry some details how change customer demands encourages innovation this sector. Nowadays, awareness clean beauty considered trend, where chemical-free, ethical, sustainable products are higher interest for most customers. It worth mention that industrial practices changing developing personalized skincare haircare products, well as, environmentally friendly packaging, use artificial intelligence product creation promotion. nanotechnology dermatology products. The helped improving active components' stability, penetration, effectiveness. Particularly useful sun protection anti-aging treatments, targeted administration ingredients Nano emulsions, liposomes, nanoparticles. offers detailed information recent developments trends industry, focusing could be used meet changes needs taking into consideration sustainability safety issues.

Language: Английский

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Wound Healing in the Modern Era: Emerging Research, Biomedical Advances, and Transformative Clinical Approaches

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 107058 - 107058

Published: May 1, 2025

Language: Английский

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Collagen III combined with autologous adipose-derived mesenchymal stem cells accelerates burn wound healing in a rat model

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(5)

Published: May 23, 2025

BACKGROUND Skin is the largest organ in human body and plays crucial roles health. Efficient rapid healing of burn wounds great significance. While stem cell therapies have offered potential methods to treat wounds, relatively few had success clinical practice. AIM To investigate effect combined application collagen III adipose-derived cells (ASCs) on vascular regeneration skin wound healing. METHODS Burn were created 18 adult male Sprague-Dawley rats, they randomly divided into two groups. In treatment group, each rat was injected with 4 × 106 Zs-Green-labeled autologous ASCs suspended III. control Each received six injections. Images acquired every 3 days. RESULTS Multiple injections improved closure rate more efficiently compared group. Moreover, do not survive long-term during process. CONCLUSION This study demonstrated that multiple accelerated by increasing deposition improving angiogenesis.

Language: Английский

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Effect of Inflammatory Microenvironment on the Regenerative Capacity of Adipose-Derived Mesenchymal Stem Cells

Cells, Journal Year: 2023, Volume and Issue: 12(15), P. 1966 - 1966

Published: July 29, 2023

Adipose-derived mesenchymal stem cells are increasingly being used in regenerative medicine as cell therapy targets, including the treatment of burns and ulcers. The potential AD-MSCs some their immunological properties known from vitro studies; however, clinical applications, non-ideal conditions can behave differently inflammatory environments, affecting efficacy outcome therapy. Our aim was to investigate map pathways that microenvironment induce these cells. High-throughput gene expression assays were performed on activated with LPS TNFα. Analysis RNA-Seq data showed control, LPS-treated TNFα-treated samples exhibited distinct patterns. increased 926 genes decreased 770 involved division, DNA repair, cycle, several metabolic processes. TNFα 174 383 genes, which related immune response, proliferation, differentiation. We also biological by further investigating most altered using Gene Ontology KEGG databases. Secreted cytokines, important examined at protein level, a functional assay assess wound healing. Activated AD-MSC secretion IL-6, IL-8 CXCL-10, closure wounds. presented accelerated healing under inflammation conditions, suggesting we could use this application.

Language: Английский

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