Recent advances in understanding the role of extracellular vesicles from probiotics in intestinal immunity signaling

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: 53(02)

Published: April 11, 2025

The diverse functions of gut symbiotic bacteria are attracting attention for their potential as probiotics. Some those release extracellular vesicles (EVs), spherical structures approximately 20–400 nm in diameter, outside cell bodies. Recent research has significantly advanced our understanding the physicochemical and biochemical properties, functions, host–cell interactions EVs released by probiotic used food fermentation, such lactic acid bacteria, bifidobacteria, butyric acetic bacteria. However, concerns have been raised regarding use these postbiotics. In this review, we discuss newly discovered roles immune signaling challenges associated with application

Language: Английский

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Intestinal Mucosal Immune Barrier: A Powerful Firewall Against Severe Acute Pancreatitis-Associated Acute Lung Injury via the Gut-Lung Axis

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 2173 - 2193

Published: April 1, 2024

The pathogenesis of severe acute pancreatitis-associated lung injury (SAP-ALI), which is the leading cause mortality among hospitalized patients in intensive care unit, remains incompletely elucidated.The intestinal mucosal immune barrier a crucial component epithelial barrier, and its aberrant activation contributes to induction sustained pro-inflammatory responses, paradoxical intercellular communication, bacterial translocation.In this review, we firstly provide comprehensive overview composition pivotal roles SAP-ALI.Secondly, mechanisms crosstalk with gut microbiota, called gut-lung axis, effect on SAP-ALI were summarized.Finally, number drugs that could enhance exhibit potential anti-SAP-ALI activities presented, including probiotics, glutamine, enteral nutrition, traditional Chinese medicine (TCM).The aim offer theoretical framework based perspective protect against SAP-ALI.

Language: Английский

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Intestinal Lymphatic Biology, Drug Delivery and Therapeutics: Current status and Future directions

Pharmacological Reviews, Journal Year: 2024, Volume and Issue: 76(6), P. 1326 - 1398

Published: Aug. 23, 2024

Historically, the intestinal lymphatics were considered passive conduits for fluids, immune cells, dietary lipids, lipid soluble vitamins and lipophilic drugs. Studies of lymphatic drug delivery in late 20^th century focussed primarily on physicochemical properties, especially high lipophilicity, that resulted transport. More recent discoveries have changed our traditional view by demonstrating are active, plastic tissue-specific players a range biological pathological processes, including within intestine. These findings have, turn, inspired exploration lymph-specific therapies diseases, as well development more sophisticated strategies to actively deliver drugs or vaccines lymph, nanotechnologies, prodrugs lipid-conjugated materials 9hitchhike9 transport pathways. With increasing novel therapeutics there has been interest whether these absorbed transported through lymph after oral administration. Here we review current state understanding anatomy physiology gastrointestinal system health disease, with focus aspects relevant delivery. We summarise state-of-the-art approaches quantify their uptake into system. Finally, excitingly, discuss examples significant pharmacokinetic therapeutic benefits achieved via also propose advance clinical application future. Significance Statement This comprehensive details It highlights shift toward use achieve patients.

Language: Английский

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Lipid-based formulations: A promising approach for poorly soluble drug delivery via the intestinal lymphatic system

Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 87, P. 104770 - 104770

Published: July 18, 2023

Language: Английский

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Lipoteichoic acid composed of poly-glycerolphosphate containing l-lysine and involved in immunoglobulin A-inducing activity in Apilactobacillus genus

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 271, P. 132540 - 132540

Published: May 21, 2024

Language: Английский

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Cell Surface Modification-Mediated Primary Intestinal Epithelial Cell Culture Platforms for Assessing Host–Microbiota Interactions

Biomaterials Research, Journal Year: 2024, Volume and Issue: 28

Published: Jan. 1, 2024

Background: Intestinal epithelial cells (IECs) play a crucial role in regulating the symbiotic relationship between host and gut microbiota, thereby allowing them to modulate barrier function, mucus production, aberrant inflammation. Despite their importance, establishing an effective ex vivo culture method for supporting prolonged survival function of primary IECs remains challenging. Here, we aim develop novel strategy support long-term response microbiota by employing mild reduction disulfides on IEC surface proteins with tris(2-carboxyethyl)phosphine. Methods: Recognizing fibroblast-IEC crosstalk, employed cell modification strategy, layer-to-layer contacts fibroblasts IECs. This involved combining negatively charged chondroitin sulfate surfaces positively chitosan thin film cells, enabling direct intercellular transfer. Validation included assessments viability, efficiency dye transfer, upon lipopolysaccharide (LPS) treatment. Results: Our findings revealed that layer-by-layer co-culture platform effectively facilitates transfer small molecules through gap junctions, providing vital viability from both intestine colon up 5 days, as evident expression E-cadherin Villin. Upon LPS treatment, these exhibited down-regulation Villin tight junction genes, such Zonula Occludens-1 , when compared nontreated counterparts. Furthermore, transcription level Lysozyme increase, while Mucin 2 showed decrease LPS, indicating responsiveness bacterial molecules. Conclusions: study provides layer-by-layer-based features, which is important understanding microbiota.

Language: Английский

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Life history of a brain autoreactive T cell: From thymus through intestine to blood-brain barrier and brain lesion

Neurotherapeutics, Journal Year: 2024, Volume and Issue: 21(6), P. e00442 - e00442

Published: Sept. 4, 2024

Language: Английский

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Probiotic Bacterium-Derived p40, p75, and HM0539 Proteins as Novel Postbiotics and Gut-Associated Immune System (GAIS) Modulation: Postbiotic-Gut-Health Axis

Microorganisms, Journal Year: 2024, Volume and Issue: 13(1), P. 23 - 23

Published: Dec. 26, 2024

It is known that probiotics have direct and indirect effects on many systems in the body, especially gastrointestinal system. Interest using probiotic strain-derived cell components metabolites has also increased as a result of significant benefits probiotics. Although terminologies definitions are used for these metabolites, International Scientific Association Probiotics Prebiotics (ISAPP) recommended use term postbiotic 2021, which defined "a preparation inanimate microorganisms and/or their confers health benefit host". Postbiotics bioactive such organic acids, peptides/proteins, wall components, functional enzymes, short-chain fatty vitamins, phenols. These molecules mediate positive immunomodulatory, antimicrobial, antioxidant effects. maintaining enabled identification new proteins p40, p75, HM0539. In this review, HM0539 derived from lactobacilli systematically summarized. The p40 protein, particular, been shown to support gut barrier activity reduce inflammation, potentially through mechanisms involving epidermal growth factor receptor-dependent signaling. Additionally, p75 exhibit protective intestinal epithelial tight junctions, suggesting therapeutic potential preventing damage diseases colitis. enhances integrity, exhibits antiinflammatory properties, protects against bacterial infection, its possible inflammatory bowel disease. This review may contribute future studies system diseases.

Language: Английский

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Biofabrication Directions in Recapitulating the Immune System‐on‐a‐Chip

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Abstract Ever since the implementation of microfluidics in biomedical field, vitro models have experienced unprecedented progress that has led to a new generation highly complex miniaturized cell culture platforms, known as Organs‐on‐a‐Chip (OoC). These devices aim emulate biologically relevant environments, encompassing perfusion and other mechanical and/or biochemical stimuli, recapitulate key physiological events. While OoCs excel simulating diverse organ functions, integration immune organs cells, though recent challenging, is pivotal for more comprehensive representation human physiology. This review covers state art intricate landscape OoC models, shedding light on role biofabrication technologies bridging gap between conceptual design relevance. The multifaceted aspects behavior, crosstalk, responses are aimed be replicated within microfluidic emphasizing need precise biomimicry explored. Furthermore, latest breakthroughs challenges platforms described, guiding researchers toward deeper understanding physiology development accurate predictive a.o., immune‐related disorders, development, programming, regulation.

Language: Английский

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Garlic Bioconverted by Bacillus subtilis Stimulates the Intestinal Immune System and Modulates Gut Microbiota Composition

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Scope This study evaluates the potential of bioconverted garlic ferments (BGFs) to stimulate intestinal immune system and modulate cecal microbiota composition. Methods results In vitro, BGF significantly enhances Peyer's patch (PP)‐mediated bone marrow cell proliferation increases production interferon‐gamma (IFN‐γ), granulocyte macrophage‐colony stimulating factor (GM‐CSF), interleukin (IL)‐6, immunoglobulin A (IgA) but not IL‐4, IL‐5, E (IgE). Oral administration C3H/HeN mice for 4 weeks GM‐CSF (42.1–45.8 pg mL −1 ) IFN‐γ (6.5–12.1 levels in PP cells. also elevates tumor necrosis factor‐alpha (TNF‐α, 165.0–236.3 mg ), (2.4–3.0 ng (1.5–3.2 small fluid, TNF‐α (2.2–3.1 (10.3–0.21.5 mouse serum. Cecal microbial analysis reveals that Bacteroidota Verrucomicrobiota decreases Actinobacteria Bacillota at phylum level mice. At genus level, abundance Fusimonas (250 kg BW day Bacteroides (125 250 Akkermansia Bifidobacterium (62.5 Limosilactobacillus ). Conclusion provides first evidence BGF's ability gut microbiota, supporting its as a novel functional material enhance immunity.

Language: Английский

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