Published: Jan. 1, 2024
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Language: Английский
Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(4)
Published: April 24, 2025
Background: Escherichia coli (E. coli) is a common opportunistic bacterial pathogen in both human and animal populations. Fatty acids serve as the central carbon energy source, process mediated by fatty acid-coenzyme A (CoA) ligases encoded fad genes such FadK. However, function mechanism of FadK remain unclear. Methods: The three-dimensional structure was modeled using AlphaFold2. After expression purification, monomeric successfully isolated. enzymatic activity assayed, real-time quantitative polymerase chain reaction (RT-qPCR) performed to quantify levels. Results: In assays acid CoA ligase activity, caprylic found be optimal substrate for We determined catalytic conditions FadK, which include pH 7.4, ATP concentration 0.6 mM, 0.8 Mg2+ mM at 37 °C. Notably, showed decrease with increasing concentrations dodecyl-AMP, further confirmed RT-qPCR results. Conclusions: Our findings will fundamental framework development innovative therapeutics that target E. infections.
Language: Английский
Citations
0
Food Microbiology, Journal Year: 2024, Volume and Issue: 122, P. 104568 - 104568
Published: May 25, 2024
Language: Английский
Citations
3
All Life, Journal Year: 2024, Volume and Issue: 17(1)
Published: June 6, 2024
The global upsurge in antibiotic resistant bacteria (ARB) is putting immense pressure on healthcare. spreading of antimicrobial resistance facilitated by mobile genetic elements, most especially plasmids. widespread use antibiotics clinical and veterinary environments creates selective that drives the evolution ARB. Plasmids contribute to propagation AR different types infections. role plasmids play this necessitates their utilization molecular surveillance detect emergence ARB track spread Recent technologies like replicon typing whole genome sequencing (WGS) have become gold standard for epidemiology detection control epidemics settings. Unfortunately, access such limited low- middle-income countries (LMICs). major aim review examine specific contributions settings elucidate various been attributed antibiotic-resistant infections healthcare Healthcare LMICs should be supported build capacity WGS effectively prevent bacterial
Language: Английский
Citations
3
Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: 37(4)
Published: Nov. 15, 2024
SUMMARYThe carbapenems remain some of the most effective options available for treating patients with serious infections due to Gram-negative bacteria. Carbapenemases are enzymes that hydrolyze and primary method driving carbapenem resistance globally. Detection carbapenemases is required patient management, rapid implementation infection prevention control (IP&C) protocols, epidemiologic purposes. Therefore, clinical public health microbiology laboratories must be able detect report among predominant organisms from both cultured isolates direct specimens treatment surveillance There not a "one size fits all" laboratory approach detection bacteria carbapenemases, institutions need determine what best goals their antimicrobial stewardship IP&C programs. Luckily, there several approaches choose methods suits individual needs. A bacterial consists two steps, namely screening process (
Language: Английский
Citations
3
Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(8)
Published: Aug. 1, 2024
Abstract Plasmids incur a fitness cost that has the potential to restrict dissemination of resistance in bacterial pathogens. However, bacteria can overcome this disadvantage by compensatory evolution maintain their resistance. Compensatory occur via both chromosomes and plasmids, but there are few reviews regarding topic, most them focus on plasmids. In review, we provide comprehensive overview currently reported mechanisms underlying elucidate key targets regulating plasmid cost, discuss future challenges field. We found primarily arises from mutations transcriptional regulatory factors, whereas plasmids predominantly involves three pathways: copy number regulation, conjugation transfer efficiency, expression antimicrobial (AMR) genes. Furthermore, importance reasonable selection research subjects effective integration diverse advanced methods is also emphasized our study mechanisms. Overall, review establishes theoretical framework aims innovative ideas for minimizing emergence spread AMR
Language: Английский
Citations
2
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175705 - 175705
Published: Aug. 23, 2024
Pathogenic Escherichia coli (PEC) are important foodborne bacteria that can cause severe illness in humans. The PECs thrive within the intestines of humans as well animals and may contaminate multiple ecosystems, including food water, via faecal transmission. Abattoir farm employees at high risk PEC exposure, which could translate to community through person-to-person contact. To determine epidemiology resistome Gauteng Limpopo provinces South Africa, 198 swine samples, 220 poultry cloacal swabs, 108 human hand 11 run-off water samples from abattoirs farms were collected four five commercial two farms. One effluent sample each was wastewater treatment plants (WWTP) a tertiary hospital setting. Phenotypic genotypic techniques used polymerase chain reaction, pulsed-field gel electrophoresis (PFGE) whole genome sequencing (WGS). Results showed EHEC EPEC prevalence 4.1 % (22/542) 20.8 (113/542), respectively, with O26 serogroup detected most isolates. According PFGE dendrogram, isolates poultry, swabs clustered together. Diverse virulence factors such novel stx2k subtype eae genes among 36 representative according WGS. results 66.7 (24/36) sequenced presented multi-drug resistance (MDR) β-lactamase 13.9 (5/36), aminoglycoside 61.1 (22/36), tetracycline 41.7 (15/36) quinolones 38.9 (14/36). No colistin nor carbapenem detected. Sequence types (STs) associated MDR this study were: ST752, ST189, ST206, ST10, ST48 ST38. findings highlight threat zoonotic pathogens close contacts need for enhanced surveillance mitigate spread PECs.
Language: Английский
Citations
2
Emerging infectious diseases, Journal Year: 2024, Volume and Issue: 30(14)
Published: Oct. 1, 2024
Surveillance of antimicrobial resistance in Kenya, Uganda, and Jordan identified multidrug-resistant high-risk bacterial clones: Escherichia coli sequence types 131, 1193, 69, 167, 10, 648, 410, 405 Klebsiella pneumoniae 14, 147, 307, 258. Clones emerging those countries exhibited high mechanism diversity, highlighting a serious threat for multidrug resistance.
Language: Английский
Citations
2
Antibiotics, Journal Year: 2023, Volume and Issue: 12(9), P. 1386 - 1386
Published: Aug. 30, 2023
A total of 199 Gram-negative bacterial isolates from urinary tract infections and 162 bloodstream were collected 12 healthcare systems throughout the United States between May 2021 August 2022. The isolates, phenotypically non-susceptible to 2nd or 3rd generation cephalosporins carbapenems, characterized through antimicrobial susceptibility testing whole genome sequence analysis obtain a broad snapshot beta-lactamase-mediated resistance among these two sample types. Overall, 23 different carbapenemase genes detected 13 species (20.5% isolates). blaKPC-3 blaKPC-2 subtypes most common identified, followed by blaNDM co-carriage blaOXA carbapenemases Acinetobacter baumannii isolates. All carbapenemase-producing A. mCIM negative. Extended-spectrum beta-lactamase identified in 66.2% isolates; blaCTX-M-15 was common. AmpC genes, both plasmid chromosomal, 33.2% Importantly, 2.8%, 8.3%, 22.2% blaKPC-positive organisms susceptible ertapenem, imipenem, meropenem, respectively. correlation broth microdilution disk diffusion results high for drugs except cefepime, where detection statistically lower diffusion. Thus, there gaps accuracy some mechanisms resistance.
Language: Английский
Citations
6
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 19, 2024
Abstract 107 isolates of Enterobacterales consisting Klebsiella pneumoniae (n=90), Escherichia coli (n=7), Enterobacter cloacae complex (n=6), oxytoca (n=3) and Citrobacter freundi (n=1) additionally an isolate Acinetobacter baumannii carrying genes encoding NDM (NDM-1, NDM-5 NDM-14), KPC (KPC-2 KPC-3), OXA-48-like (OXA-48, OXA-181 OXA-232) IMP (IMP-1 IMP-4) carbapenemases were sequenced using q20 nanopore chemistry to provide complete/near-complete assemblies relevant plasmids compared. Investigation potential ‘plasmid outbreaks’ in individual hospitals among different types species revealed a mixed situation with some similar plasmids, but segments missing/added that clearly distinct. While most bla OXA-48 typical IncL approximately 60 kb are widely described, there was variation these. One carried IncR plasmid had only limited homology the others. Identical 51,479 bp ColKP3/IncX3 found from exactly matched those on GenBank other countries. In IncFII plasmids. OXA-232 highly conserved small ColKP3 countries continents. These observations highlight importance understanding wider distribution concern. IncHI2/IncHI2A important vehicles for carbapenemase KPC-2 , IMP-1 IMP-4 or NDM-1 sometimes colistin resistance gene mcr-9 addition. Representatives K. sequence type (ST) 147 seven IncFIB(pNDM-Mar)/IncHI1B(pNDM-MAR) hybrid virulence 325 352 combined A also ST1558 has been described representatives ST383. Nanopore sequencing instrumental improving our knowledge leading better their epidemiology.
Language: Английский
Citations
1
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: April 29, 2024
There is scarce information concerning the role of sporadic clones in dissemination antimicrobial resistance genes (ARGs) within nosocomial niche. We confirmed that clinical Escherichia coli M19736 ST615 strain, one first isolates Latin America harbors a plasmid with an mcr-1 gene, could receive crucial ARG by transformation and conjugation using as donors critical plasmids harbor bla CTX-M-15 , KPC-2 NDM-5 NDM-1 or aadB genes. acquired genes, being only blaNDM-1 maintained at 100% on 10th day subculture. In addition, when evolved MDR- E. sequentially maintenance pattern changed. XDR- ulterior step paadB plasmid, different plasmid’s was found. Interestingly, strains disseminated simultaneously conjugative combinations though selection ceftazidime all cases. Finally, we isolated characterized extracellular vesicles (EVs) from native strains. EVs harbored pDCAG1-CTX-M-15 previously lost shown WGS experiments, suggesting EV be relevant reservoir for susceptible bacteria. These results evidenced genetic plasticity clone such play transitional link dynamics evolution to multidrug/extensively/pandrug-resistant phenotypes superbugs niche acting vector multiple ARGs which later disseminated.
Language: Английский
Citations
1