X-ray Fluorescence Microscopy to Develop Elemental Classifiers and Investigate Elemental Signatures in BALB/c Mouse Intestine a Week after Exposure to 8 Gy of Gamma Rays DOI Open Access
A. Gordon Smith,

Katrina Dobinda,

Si Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10256 - 10256

Published: Sept. 24, 2024

Iron redistribution in the intestine after total body irradiation is an established phenomenon. However, literature, there are no reports about use of X-ray fluorescence microscopy or equivalent techniques to generate semi-quantitative 2D maps iron sectioned samples from irradiated mice. In this work, we used (XFM) map elemental content as well phosphorus, sulfur, calcium, copper and zinc tissue sections small eight-week-old BALB/c male mice that developed gastrointestinal acute radiation syndrome (GI-ARS) response exposure 8 Gray gamma rays. Seven days irradiation, found majority localized hot spots intercellular regions area surrounding crypts stretching between outer perimeter surface cell layer villi. addition, study represents our current efforts develop classifiers could be for automated generation interest analyses maps. Once developed, such a tool will instrumental studies effects other toxicants on cells tissues. While XFM cannot conducted living organisms, it possible envision future scenarios where imaging single sloughed human (or rodent) follow up progression GI-ARS.

Language: Английский

Homeostasis and metabolism of iron and other metal ions in neurodegenerative diseases DOI Creative Commons
Leilei Chen, Qingqing Shen, Yingjuan Liu

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: Feb. 2, 2025

Language: Английский

Citations

3
BIOMETALS IN NEURODEGENERATIVE DISEASES: THEIR BENEFICIAL AND DETRIMENTAL ROLES IN BRAIN HEALTH. DOI Open Access

TOBILOLA ODUNAYO AKINGBADE,

ELIJAH AYODELE OJO,

OPEYEMI PETER ADEBISI

et al.

Greener Journal of Biomedical and Health Sciences, Journal Year: 2025, Volume and Issue: 8(1), P. 16 - 27

Published: March 10, 2025

Biometals are essential to many physiological functions, including the normal functioning and development of brain. Notably, altered levels these biometals in body have been observed patients living with neurodegenerative conditions like Alzheimer’s, Huntington’s, Parkinson’s, Amyotrophic Lateral Sclerosis. This review will establish how can enter accumulate mechanisms for exhibiting neurotoxic effects. Correlations between level other non-essential metals existence diseases make them worthy study. Researchers looked toward biometal homeostasis as potential sources biomarkers, diagnostic breakthroughs, therapeutic solutions conditions. In addition, we explore benefit brain health, their role conditions, next frontiers biometal-based diagnostics therapy research.

Language: Английский

Citations

0
Recent advancements in the therapeutic approaches for Alzheimer's disease treatment: Current and future perspective DOI Creative Commons
Amit Sharma, Santosh Rudrawar, Sandip B. Bharate

et al.

RSC Medicinal Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

This review collates the recent information related to proposed hypotheses for AD etiology and advances in various therapeutic options, with a particular emphasis on clinical candidates.

Language: Английский

Citations

2
Emerging paradigms in Alzheimer's therapy DOI

Agnes Preethy H.,

Kayalvizhi Rajendran,

Anitha Josephine Sukumar

et al.

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 981, P. 176872 - 176872

Published: Aug. 6, 2024

Language: Английский

Citations

1
Neuroprotective Potential of Indole-Based Compounds: A Biochemical Study on Antioxidant Properties and Amyloid Disaggregation in Neuroblastoma Cells DOI Creative Commons
Tania Ciaglia, Maria Rosaria Miranda, Simone Di Micco

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1585 - 1585

Published: Dec. 23, 2024

Based on the established neuroprotective properties of indole-based compounds and their significant potential as multi-targeted therapeutic agents, a series synthetic indole–phenolic was evaluated multifunctional neuroprotectors. Each compound demonstrated metal-chelating properties, particularly in sequestering copper ions, with quantitative analysis revealing approximately 40% chelating activity across all compounds. In cellular models, these hybrid exhibited strong antioxidant cytoprotective effects, countering reactive oxygen species (ROS) generated by Aβ(25–35) peptide its oxidative byproduct, hydrogen peroxide, showing average 25% increase cell viability reduction ROS levels to basal states. Further using thioflavin T fluorescence assays, circular dichroism, computational studies indicated that synthesized derivatives effectively promoted self-disaggregation fragment. Taken together, findings suggest unique profile actions for derivatives, combining chelating, antioxidant, anti-aggregation which position them promising development agents Alzheimer’s disease therapy. The methods used provide reliable vitro data, although further vivo validation assessment blood–brain barrier penetration are needed confirm efficacy safety.

Language: Английский

Citations

1
X-ray Fluorescence Microscopy to Develop Elemental Classifiers and Investigate Elemental Signatures in BALB/c Mouse Intestine a Week after Exposure to 8 Gy of Gamma Rays DOI Open Access
A. Gordon Smith,

Katrina Dobinda,

Si Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10256 - 10256

Published: Sept. 24, 2024

Iron redistribution in the intestine after total body irradiation is an established phenomenon. However, literature, there are no reports about use of X-ray fluorescence microscopy or equivalent techniques to generate semi-quantitative 2D maps iron sectioned samples from irradiated mice. In this work, we used (XFM) map elemental content as well phosphorus, sulfur, calcium, copper and zinc tissue sections small eight-week-old BALB/c male mice that developed gastrointestinal acute radiation syndrome (GI-ARS) response exposure 8 Gray gamma rays. Seven days irradiation, found majority localized hot spots intercellular regions area surrounding crypts stretching between outer perimeter surface cell layer villi. addition, study represents our current efforts develop classifiers could be for automated generation interest analyses maps. Once developed, such a tool will instrumental studies effects other toxicants on cells tissues. While XFM cannot conducted living organisms, it possible envision future scenarios where imaging single sloughed human (or rodent) follow up progression GI-ARS.

Language: Английский

Citations

0