Cellular Microbiology,
Journal Year:
2024,
Volume and Issue:
2024(1)
Published: Jan. 1, 2024
Pseudomonas
aeruginosa
,
an
antibiotic‐resistant
opportunistic
pathogen,
poses
significant
challenges
in
treating
infections,
particularly
immunocompromised
individuals.
This
review
explores
current
and
future
innovative
approaches
to
suppress
its
growth
virulence.
We
delve
into
the
bacterium’s
virulence
factors,
discussing
existing
strategies
like
antibiotics,
bacteriophages,
probiotics,
small‐molecule
inhibitors.
Additionally,
novel
approaches,
including
RNA
interference,
CRISPR‐Cas
systems,
nanotechnology,
show
promise
preclinical
studies.
Despite
advancements,
persist,
prompting
need
for
a
multifaceted
approach
targeting
various
aspects
of
P.
pathogenesis
effective
infection
management.
provides
comprehensive
perspective
on
status
directions
against
aeruginosa.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
6(8)
Published: July 24, 2024
Abstract
In
the
recent
decade,
zinc
oxide
nanoparticles
(ZnO
NPs)
have
been
widely
explored
owing
to
their
versatile
properties
and
prodigious
demands
in
drug
delivery,
medical,
energy
storage,
cosmetics,
healthcare
sectors.
Therefore,
current
work
opts
for
an
environmentally
benign
method
prepare
ZnO
NPs.
The
leaf
extract
of
Calendula
officinalis
L.
acts
as
a
reducing
agent
metal
ions;
therefore,
research,
NPs
were
prepared
via
green
route
by
using
extract.
Furthermore,
analysed
with
different
spectroscopic
techniques
confirm
structure
stability
nanomaterials.
characterized
XRD,
FE-SEM,
FT-IR
UV–Vis
studies.
Also,
antioxidant
antimicrobial
synthesized
investigated.
XRD
result
showed
crystalline
size
28.23
nm
wurtzite
hexagonal
along
most
intense
peak
(101).
Following
preliminary
confirmations
intended
NPs,
both
big
small
agglomerated
forms
observed
which
is
often
used
determine
exterior
assembly.
Further,
results
Fourier
transform
infrared
spectroscopy
(FT-IR)
indicated
formation
pure
absorption
Zn–O
bond
between
4000
cm
−1
500
no
discernible
monitoring
range.
spectrum
revealed
two
prominent
peaks
at
355
370
band
gap
2.986
eV.
Using
1,
1-di
phenyl-2-picrylhydrazyl
(DPPH)
test,
activity
described
was
assessed.
It
demonstrated
how,
significantly
increased
scavenging
free
radicals.
could
be
seen
that
synthesis
naturally
occurring
plant
product
acting
alternate
chemical
antioxidant.
analysis
also
performed
help
disk
diffusion
where
three
multi-drug
resistant
human
pathogens
namely
Staphylococcus
aureus,
Klebsiella
pneumoniae
E.coli
used.
Zone
Inhibition
diameter
values
are
35.2
mm
±
0.9,
23.6
0.1
13.5
0.1,
respectively,
highly
effective
against
S.
aureus.
Thus,
evidence
it
superior
friendly
preparation
hence
can
utilized
various
nano-medicine
approaches.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
6(3)
Published: March 8, 2024
Abstract
Nowadays,
extended
spectrum
β-lactamase
(ESBL)
producing
Escherichia
coli
has
been
recognized
and
recorded
worldwide
as
one
of
the
main
causing
agents
a
major
contributor
to
nosocomial
infections.
The
current
study
aimed
isolate
detect
β-lactamase-producing
E.
use
it
in
extracellular
biosynthesis
iron
oxide
nanoparticles
(Fe
2
O
3
NPs).
Fifteen
Gram-negative
(G-ve),
lactose-fermenting,
negative
citrate
non-spore-forming
coliform
bacteria
were
isolated
from
total
bacterial
isolates
water
samples.
Different
tests
performed
including
chromogenic
methods
acidimetric
iodometric
techniques
phenotypic
cloverleaf
test
Masuda
double-disc
test.
ESBL-producing
was
detected
confirmed
by
modified
double
disc
synergy
using
ceftazidime,
cefotaxime,
ceftriaxone,
amoxicillin
combined
with
clavulanic
acid,
imipenem,
cefepime,
cefoxitin.
quantitative
assay
done
micro-iodometric
assay.
Among
isolates,
S1B1
(the
highest
activity)
selected
tested
for
Fe
NPs.
produced
(NPs)
characterized
UV–visible
spectroscopy,
X-ray
diffraction
analysis,
Fourier-transform
infrared
spectroscopy
(FTIR),
transmission
electron
microscope
Zeta
analysis.
Results
successful
NPs
which
displayed
an
absorption
peak
at
346
nm
crystallographic
lattice
plane
(104).
negatively
charged
spherical-shaped
average
size
≈
24
±
nm.
FTIR
refers
presence
NPs-associated
proteins
act
stabilizing
capping
agents.
Antibacterial
activity
against
Staphylococcus
aureus
ATCC25923,
Bacillus
cereus
ATCC6633
(G+ve
bacterium),
Pseudomonas
aeruginosa
ATCC27853
(G-ve
well
strain.
revealed
moderate
strong
antibacterial
action
strains
minimum
inhibition
concentration
(MIC)
ranging
25
40
µg/ml.
BMC Chemistry,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Feb. 17, 2024
Abstract
Nanocomposites
incorporating
titanium
dioxide
(TiO
2
)
have
a
significant
potential
for
various
industrial
and
medical
applications.
These
nanocomposites
exhibit
selectivity
as
antimicrobial
anticancer
agents.
Antimicrobial
activity
is
crucial
uses,
including
applications
in
food
processing,
packaging,
surgical
instruments.
Additionally,
these
A
stable
nanocomposite
new
antibacterial
chemical
was
prepared
by
coupling
nanoparticles
with
polyurethane
foam
matrix
through
the
thiourea
group.
The
dioxide/thiopolyurethane
(TPU/TiO
synthesized
from
low-cost
Ilmenite
ore
commercial
foam.
EDX
analysis
used
to
determine
elemental
composition
of
matrix.
TiO
NPs
were
characterized
using
TEM,
XRD,
IR,
UV–Vis
spectra.
TPU
formed
novel
composite.
MTT
assay
assessed
Cisplatin
HepG-2
MCF-7
cytotoxicity
vitro.
Its
IC
50
values
122.99
±
4.07
201.86
6.82
µg/mL,
respectively.
TPU/TiO
exhibits
concentration-dependent
against
cells
selective
index
measured
both
cell
lines;
it
showed
its
safety
healthy
cells.
Agar
well-diffusion
exhibited
good
inhibition
zones
Escherichia
coli
(12
mm),
Bacillus
cereus
(10
Aspergillus
niger
(19
mm).
TEM
-treated
bacteria
ultrastructure
changes,
plasma
membrane
detachment
wall,
which
caused
lysis
bacterial
death.
can
treat
cancer
inhibit
microbes
dentures
other
items.
Also,
inhibits
E.
,
B.
cereus,
A.
microbial
strains.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(17), P. 9500 - 9500
Published: Aug. 31, 2024
Nanotechnology
has
gained
popularity
in
recent
years
due
to
its
wide-ranging
applications
within
the
scientific
community.
The
three
main
methods
for
synthesizing
nanoparticles
are
physical,
chemical,
and
biological.
However,
adverse
effects
associated
with
physical
chemical
have
led
a
growing
interest
biological
methods.
Interestingly,
green
synthesis
using
plants
prominence
developing
new
treatments
bacterial
infections.
Zinc
oxide
(ZnO
NPs)
produced
environmentally
friendly
more
biocompatible
potential
as
antibacterial
agents
biomedical
field.
As
result,
this
review
discusses
of
ZnO
NPs,
factors
influencing
optimal
synthesis,
characterization
techniques,
activity
some
plant-mediated
NPs.
It
also
provides
comprehensive
analytical
exploration
NP
biosynthesis,
role
phytochemical
compounds
reducing
stabilizing
agents,
mechanism
action
their
properties
further
highlights
challenges
prospects
innovative
research
area.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 21, 2024
The
threat
of
antibiotic
resistance
is
escalating,
diminishing
the
effectiveness
numerous
antibiotics
due
to
rapid
development
resistant
bacteria.
In
response,
use
green-synthesized
nanoparticle,
alone
or
combined
with
antimicrobial
agents,
appears
promising.
This
study
explores
zinc
oxide
nanoparticles
(ZnONPs)
synthesized
using
Loranthus
cordifolius
leaf
extracts
and
subsequently
coated
anethole.
fabrication
these
was
confirmed
via
UV-Vis,
FTIR
TEM
analyses,
ensuring
were
produced
as
intended.
Utilizing
a
nanoprecipitation
process
that
excludes
evaporation
drying,
high
drug
loading
capacity
16.59%
accomplished.
encapsulation
efficiency
for
anethole
recorded
at
88.23
±
4.98%.
Antibacterial
efficacy
assessed
by
com
paring
ZnONPs
(average
size:
14.47
nm),
anethole-loaded
14,75
commercially
sourced
ZnONPs.
demonstrated
superior
inhibition
against
all
tested
bacterial
strains,
including
Gram-negative
species
like
Pseudomonas
aeruginosa
Escherichia
coli,
Gram-positive
Bacillus
subtilis
Staphylococcus
aureus,
outperforming
available
Additionally,
anethole-coated
showed
greatest
Gyr-B
activity
(IC50
=
0.78
0.2
M),
better
than
both
These
findings
emphasize
enhanced
properties
ZnONPs,
particularly
when
green
synthesis
loading,
highlighting
their
potential
in
various
biomedical
applications.
