Environment International,
Journal Year:
2024,
Volume and Issue:
183, P. 108435 - 108435
Published: Jan. 1, 2024
The
presence
of
polycyclic
aromatic
hydrocarbons
(PAHs)
in
soil
negatively
affects
the
environment
and
degradation
these
contaminants
is
influenced
by
nitrogen
metabolism.
However,
mechanisms
underlying
interrelationships
between
functional
genes
involved
metabolism
phenanthrene
(PHE)
biodegradation,
as
well
effects
biochar
on
mechanisms,
require
further
study.
Therefore,
this
study
utilised
metabolomic
metagenomic
analysis
to
investigate
primary
processes,
associated
enzymes
genes,
differential
metabolites
PHE-contaminated
with
without
amendment
over
a
45-day
incubation
period.
Results
showed
that
dissimilatory
nitrate
reduction
ammonium
(DNRA)
denitrification
were
dominant
processes
soil.
addition
enhanced
modules,
exhibiting
discernible
temporal
fluctuations
DNRA
proportions.
Co-occurrence
networks
correlation
heatmap
revealed
potential
interactions
among
responsible
for
PHE
biodegradation
Notably,
displayed
significant
positive
downstream
degradation.
Of
particular
interest
was
stronger
observed
biochar.
inhibited
9-phenanthrol
pathway,
resulting
elevated
levels
glutathione
(GSH)
response
environmental
stress.
These
findings
provide
new
insights
into
highlight
dual
processes.
Ecotoxicology and Environmental Safety,
Journal Year:
2025,
Volume and Issue:
292, P. 117974 - 117974
Published: March 1, 2025
Cottonseed
meal
is
widely
used
as
an
alternative
source
of
protein
in
the
animal
feed
industry.
However,
presence
toxic
gossypol
limits
its
use
livestock
production.
In
order
to
reduce
toxicity,
microbial
degradation
generally
considered
be
environmentally
friendly
and
cost-effective
strategy.
Candida
tropicalis
ZD-3
has
demonstrated
ability
degrade
gossypol.
Nevertheless,
genome
gossypol-induced
C.
not
been
fully
sequenced,
comprehensive
metabolic
profile
remains
unexplored.
this
study,
rate
by
reached
88.5
%,
determined
high
performance
liquid
chromatography
(HPLC).
The
characteristic
peaks
amides
were
changed
after
treatment
Fourier
transform
infrared
spectroscopy
(FTIR)
analysis.
Genomic
correlation
results
showed
that
gene
function
annotation
revealed
64
protein-coding
genes
potentially
involved
catabolism,
primarily
encoding
aldehyde
dehydrogenase,
reductase,
glutathione
peroxidase.
Metabolomic
analysis
indicated
activated
ABC
transporters
amino
acid
synthesis
pathways,
such
histidine,
lysine,
arginine
biosynthesis.
These
pathways
provided
substantial
energy
for
cells
cope
with
external
stress,
promoted
tricarboxylic
(TCA)
cycle,
formed
a
complex
regulatory
network
tolerance
degradation.
This
study
marks
first
revelation
metabolism
tropicalis,
laying
foundation
further
research
on
detoxification.
