Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 7, 2023
Abstract The full text of this preprint has been withdrawn by the authors while they make corrections to work. Therefore, do not wish work be cited as a reference. Questions should directed corresponding author.
Language: Английский
Reproductive Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 30, 2025
Language: Английский
Citations
1
Metabolites, Journal Year: 2024, Volume and Issue: 14(1), P. 36 - 36
Published: Jan. 5, 2024
This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, cerebrospinal fluid) under the perspective clinical metabolomics. These samples are interesting metabolomics studies as they reflect status living organisms can be applied diagnostic purposes biomarker discovery. Pre-collection procedures critical, requiring protocols to standardized avoid contamination bias. Such must consider cleaning area, stimulation, diet, food drug intake, among other that impact lack homogeneity group. Precipitation proteins removal salts cell debris most used preparation procedures. intends provide a global view practical aspects results, serving starting point designing metabolomic experiments.
Language: Английский
Citations
5
Theriogenology, Journal Year: 2025, Volume and Issue: unknown, P. 117395 - 117395
Published: March 1, 2025
Language: Английский
Citations
0
Asian Journal of Andrology, Journal Year: 2025, Volume and Issue: unknown
Published: April 8, 2025
Varicocele (VC) is a common cause of male infertility, yet there lack molecular information for VC-associated infertility. This study investigated alterations in the seminal plasma metabolomic and lipidomic profiles infertile VC patients. Twenty males with twenty-three age-matched healthy controls (HCs) were recruited from Peking Union Medical College Hospital (Beijing, China) between October 2019 April 2021. Untargeted metabolite lipid analyzed using mass spectrometry. Four hundred seventy-six metabolites seventeen lipids significantly different patients compared to HCs. The top enriched pathways among these are protein digestion absorption, aminoacyl-transfer RNA (tRNA) biosynthesis, biosynthesis amino acids. Different key species, including triglyceride (TG), diacylglycerol (DG), ceramides (Cer), phosphatidylserine (PS), varied HC groups. distinct moderately correlated. DL-3-phenyllactic acid potential diagnostic biomarker VC-related infertility (area under curve [AUC] = 0.893), positively correlating sperm count, concentration, motility. Furthermore, only shared by all four comparisons (VC vs HC, VC-induced oligoasthenospermia [OAS] asthenospermia [AS], OAS AS HC). decreased than AS. Metabolite-targeting gene analysis revealed carbonic anhydrase 9 (CA9) might be strongest candidate associated onset severity VC. differ those could promising biomarker.
Language: Английский
Citations
0
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 340, P. 126237 - 126237
Published: April 28, 2025
Language: Английский
Citations
0
Andrology, Journal Year: 2023, Volume and Issue: 12(4), P. 719 - 739
Published: Oct. 10, 2023
Abstract The incidence of male infertility (MI) is rising annually. According to epidemiological studies, environmental pollution (e.g., organic, inorganic, and air pollutants), occupational exposure high temperature, organic solvents, pesticides), poor lifestyle diet, sleep, smoking, alcohol consumption, exercise) are important non‐genetic causative factors MI. Due multiple complex factors, the dose–effect relationship, uncertainty pathogenicity, pathogenesis MI far from fully clarified. Recent data show that can be monitored by metabolites in serum, seminal plasma, urine, testicular tissue, sperm, other biological samples. It considered these closely related phenotypes directly reflect individual pathological physiological conditions. Therefore, qualitative quantitative analysis metabolome, metabolic pathways, identification biomarkers will help explore MI‐related problems provide valuable insights into its pathogenic mechanisms. Here, we summarized new findings metabolomics research their abnormal pathways triggered presented risk providing a landscape semen plasma general patients. Then, compared similarities differences between patients exposed general, some common biomarkers. We better understanding underpinnings pathogenesis, which might offer novel diagnostic, prognostic, precise treatment approaches
Language: Английский
Citations
6
Journal of Reproduction & Infertility, Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 25, 2023
Background: Male infertility is usually determined by the manual evaluation of semen, namely standard semen analysis. It currently impossible to predict sperm fertilizing ability based on analysis alone. Therefore, a more sensitive and selective diagnosis tool required. Methods: Twelve fresh samples were collected from fertile volunteers attending Avicenna Fertility Center (Tehran, Iran). The seminal plasma (SP) was prepared subjected liquid chromatography-tandem mass spectrometry (LC-MS/MS), total antioxidant capacity (TAC) Thirty-four amino acids including essential (EAA), non-essential (NEAA), non-proteinogenic (NPAA) relative concentration determined, correlation between their with spermiogram parameters TAC SP analyzed. Results: Significant positive correlations have been found selected motility (Met Gln, rs=0.92; Cys, rs=0.72; Asn, rs=0.82), normal morphology (Met, Glu, (Trp, Phe, Ala). In contrast, several AAs, Gly, Ser, Ile showed negative (rs=-0.93, r=-0.92, r=-0.89, respectively). Furthermore, association only Tyr (rs=0.79). Conclusion: strong positive/negative metabolic signature demonstrate significance determining metabolite levels under conditions for functions. Combining metabolome clinical characteristics would enable clinicians look beyond biomarkers toward interpretation explain biological basis pathology.
Language: Английский
Citations
4
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0303189 - e0303189
Published: May 20, 2024
Objectives To establish a rat model that accurately replicates the clinical characteristics of male infertility (MI) with Liver Depression and Kidney Deficiency (LD & KD) investigate pathogenesis. Methods After subjecting rats to chronic restraint stress (CRS) adenine treatment, series tests were conducted, including ethological assessments, evaluations reproductive characteristics, measurements biochemical parameters, histopathological examinations, analyses urinary metabolites. Additionally, bioinformatics predictions performed for comprehensive analysis. Results Compared control, exhibited significant manifestations MI LD KD, reduced responsiveness, diminished frequency capturing estrous female rats, absence mounting behavior. kidney coefficient increased markedly, while coefficients testis epididymis decreased significantly. Sperm counts viabilities notably, accompanied by an increase in sperm abnormalities. Dysregulation hormone levels serum was observed, upregulation proinflammatory cytokines expressions liver kidney, as well exacerbated oxidative penile corpus cavernosum testis. The seminiferous tubules loose arrangement, loss germ cells, infiltration inflammatory cells. Furthermore, utilizing metabolomics analysis, 5 key biomarkers 2 crucial targets most closely linked revealed. Conclusion study successfully established clinically relevant animal KD. It elucidates pathogenesis condition, identifies targets, provides robust scientific foundation prediction, diagnosis, treatment
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Oct. 10, 2024
Male infertility is one of the most common reproductive dysfunctions. Despite oligospermia being a cause infertility, few studies have been conducted on it. This study aimed to investigate differences in semen metabolic patterns patients with and identify potential biomarkers associated oligospermia. Semen samples from (20 cases) healthy controls were detected by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), 72 89 metabolites identified as markers positive negative ion modes, respectively. In addition, results multiple pathways oligospermia, such glycine serine threonine metabolism, Synthesis degradation ketone bodies, Valine, leucine, isoleucine degradation. These described unique characteristics provided novel insights into mechanism disorder.
Language: Английский
Citations
1
Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 185, P. 114463 - 114463
Published: Jan. 21, 2024
Language: Английский
Citations
0