Protective effects of melatonin against physical injuries to testicular tissue: A systematic review and meta-analysis of animal models

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 31, 2023

Background Modern societies face infertility as a global challenge. There are certain environmental conditions and disorders that damage testicular tissue may cause male infertility. Melatonin, potential antioxidant, protect tissue. Therefore, we conducted this systematic review meta-analysis to evaluate the effects of melatonin in animal models against physical, heat, ischemic Methods PubMed, Scopus, Web Science were systematically searched identify trials evaluating protective effect therapy on rodent when it is exposed thermal, ischemic, or hypobaric oxygen stress. Random-effect modeling was used estimate standardized mean difference 95% confidence intervals based pooled data. Additionally, Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) tool assess risk bias. The study protocol prospectively registered PROSPERO (CRD42022354599). Results A total 41 studies eligible out 10039 records. Studies employed direct cryptorchidism, varicocele, torsion-detorsion, vascular occlusion, hypoxia, ischemia-reperfusion, stress by excessive restraint activity, spinal cord injury, trauma induce subjects. histopathological characteristics generally improved rodents therapy. Based data, sperm count, morphology, forward motility, viability, Johnsen’s biopsy score, glutathione peroxidase, superoxide dismutase levels higher treatment arms. In contrast, malondialdehyde level lower included suffered from high bias most SYRCLE domains. Conclusion This concludes associated with characteristics, reproductive hormonal panel, markers oxidative thermal injuries. regard, deserves scientific investigations drug registration https://www.crd.york.ac.uk/PROSPERO/ , identifier CRD42022354599.

Language: Английский

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Sea buckthorn, its bioactive constituents, and mechanism of action: potential application in female reproduction

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 7, 2023

Sea buckthorn ( Hippophae rhamnoides L.) is a flowering shrub, and its berries have been utilized for decades as raw ingredient in cuisines herbal remedies. This evidence-based study focuses on key bioactive constituents, mechanism of protective effects with focus female reproductive processes. Parts the plant contain phenols, carotenoids (lycopene, carotene, lutein, zeaxanthin), flavonoids (isorhamnetin, quercetin, glycosides, kaempferol), tocopherols, sterols, polyunsaturated fatty acids, minerals, vitamins, omega 3, 6, 9 rare 7 acids etc. Key polyphenolic such isorhamnetin quercetin are believed to be mainly responsible behind health benefits (against cardiovascular diseases, metabolic syndrome, obesity etc.) through properties including anti-cancer, antioxidant, anti-inflammatory activities. These sea constituents appear mediate healthy ovarian cell proliferation, death, hormone release, well decrease cancer possibly apoptosis, hormonal (estrogen) release. Thus, ingredients may potential management gynecological problems uterine inflammation, endometriosis, easing symptoms vulvovaginal atrophy postmenopausal women (by targeting inflammatory cytokines vascular endothelial growth factor – VEGF). Apigenin, myricetin, luteolin also recommended prospective preventative adjuvant therapy options they can inhibit cancerogenesis by triggering apoptosis halting cycle tumors. Furthermore, oil (containing carotenoid, sterol, hypericin) has speculated an alternative estrogen replacement particularly improve vaginal epithelial integrity. However, it uncertain whether steroid receptors, reactive oxygen species (ROS), regulators actually buckhorn’s actions. buckthorn, compounds’ promoting warrants further validation not just vitro animal research, but clinical trials identify and/or standardize optimal methods delivery biologically active molecules.

Language: Английский

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Curcumin‐loaded nanoparticles effectively prevent T₄‐induced oxidative stress in rat heart

Cell Biochemistry and Function, Journal Year: 2024, Volume and Issue: 42(4)

Published: June 1, 2024

Abstract In this study, we report the cardioprotective effect of glycerol monooleate (GMO) based nanocurcumin in both vitro and vivo conditions under a hyperthyroid state. The heart is one primary target organs sensitive to action thyroid hormone, slight variations hormone serum concentrations result measurable changes cardiac performance. Hyperthyroidism‐induced hypermetabolism associated with oxidative stress an important mechanism responsible for progression failure. Curcumin has been known play protective role against stress‐related diseases like Alzheimer's, asthma, aging due its antioxidant properties. Nevertheless, potent biological activity hindered poor bioavailability. To overcome drawback, GMO‐based biodegradable nanoparticle (NP) formulation loaded curcumin developed, curcumin‐loaded NPs was compared native drug. Oxidative parameters reactive oxygen species (ROS) release, change mitochondrial membrane permeability, lipid peroxidation (LPx), lactate dehydrogenase (LDH) protein expression endogenous enzymes superoxide dismutase, catalase (CAT) glutathione peroxidase were evaluated. results from showed that better DPPH NO radical scavenging than range 2.5–20 µM. It also observed nanoparticulate comparatively more effective protecting ROS‐induced damage by reducing LPx LDH leakage at low 5–10 Further, performed facilitating activities respect time concentrations, resulting reduced cellular ROS levels. scenario, anticipate can serve as counterpart diseases.

Language: Английский

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The Role of Reductive Stress in the Pathogenesis of Endocrine-Related Metabolic Diseases and Cancer

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1910 - 1910

Published: Feb. 23, 2025

Reductive stress (RS), characterized by excessive accumulation of reducing equivalents such as NADH and NADPH, is emerging a key factor in metabolic disorders cancer. While oxidative (OS) has been widely studied, RS its complex interplay with endocrine regulation remain less understood. This review explores molecular circuits bidirectional crosstalk between hormones RS, focusing on their role diabetes, obesity, cardiovascular diseases, disrupts insulin secretion signaling, exacerbates inflammation, contributes to adipose tissue dysfunction, ultimately promoting resistance. In alters vascular smooth muscle cell function myocardial metabolism, influencing ischemia-reperfusion injury outcomes. cancer, plays dual role: it enhances tumor survival buffering OS reprogramming, yet can trigger proteotoxicity mitochondrial leading apoptosis. Recent studies have identified RS-targeting strategies, including redox-modulating therapies, nanomedicine, drug repurposing, offering potential for novel treatments. However, challenges remain, particularly distinguishing physiological from pathological conditions overcoming therapy-induced Future research should focus developing selective biomarkers, optimizing therapeutic interventions, exploring the immune regulation.

Language: Английский

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Editorial: Gastrointestinal (GI) disorders and antioxidant therapeutics

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

association between OBS and IBD. demonstrated a negative correlation with IBD, particularly among female Crohn's disease (CD) patients. This research highlights the importance of an antioxidant diet lifestyle, potentially offering improved health for patients CD. The study conducted by Chang et al. indicates that increased exposure to antioxidants, evaluated through (diet lifestyle rich in antioxidants), could reduce incidence colorectal cancer (CRC). A significant was identified likelihood developing CRC its various subsites (proximal colon cancer, distal rectal cancer). especially evident male Biomarkers are essential detection diseases evaluating effectiveness treatments (4,5). Serum albumin, uric acid, neutrophil count biomarkers mediating potential CRC.Antioxidants have been suggested as possible alternative treatment antiinflammatory/immunomodulatory medications used treat GI disorders. There types each exhibiting distinct mechanisms action application specific clinical applications (6). Natural compounds can scavenge ROS enhance body's defense (6)(7)(8)(9)(10)(11)(12)(13), inhibiting pro-oxidative enzymes aiding When capacity damaged mucosa is diminished, nontraditional therapies, including pharmaceuticals, natural or synthetic agents, hormones, probiotics neutralize reactive oxygen nitrogen species, mitigate cellular damage (protein carbonylation, lipid peroxidation, DNA modification) function enzymes, helpful when conjunction anti-inflammatory independently (14)(15)(16)(17). Muro provided comprehensive overview involvement OS pathophysiology highlighting diagnostic targets exploring use therapies manage Markers OS, 8-hydroxy-2'deoxyguanosine, malondialdehyde, serum-free thiols, were blood stool correlated severity. Consequently, markers serve dual purpose, both diagnosis assessment IBD efficacy. also focus on such glutathione, vitamin C, E, N-acetylcysteine. In their review, Sahoo examined range polyphenolic substances, curcumin, resveratrol, quercetin, caffeic acid phenethyl ester, green tea flavonoids, luteolin, genistein, xanthohumol, alpinetin, silymarin, proanthocyanidins, anthocyanins phenolic thymol, alkaloids like berberine, storage polysaccharides tamarind xyloglucan, phytochemicals represented isothiocyanate sulforaphane food/spices ginger, flaxseed oil, along hormones melatonin, all which target signaling pathways intestinal inflammation associated He relationship dietary carotenoid intake soluble Klotho (S-Klotho) plasma levels elderly adults. overall consumption carotenoids showed positive concentrations S-Klotho population, ingestion α-carotene, β-carotene, lutein zeaxanthin. gut microbiota (GM), includes bacteria, archaea, fungi, protozoa, viruses, significantly influences host (18,19). GM plays crucial role epithelial barrier, aids metabolism, offers protection against pathogens, impacts development mucosal immune system (19). Numerous findings illustrate prebiotics, probiotics, postbiotics, synbiotics human animal health, contributing maintenance healthy microbiota, enhancing promoting tolerance, helping regulate proinflammatory response (18,(20)(21)(22). underlying functional disorders encompass alterations microbiota/gut hormone axis, play motility. Hormone receptors discovered inflamed regions, demonstrating ability trigger pro-and responses. Dai review pathogenesis cardiovascular (CVD), addressing factors inflammatory response, mitochondrial dysfunction, pyroptosis, ferroptosis, dysbiosis GM. Research functions "microbial organ" influencing "gut-heart" axis presents promising pathway preventing treating CVD. number potent derived from traditional Chinese medicine (TCM) identified, acids, stilbenes, saponins, anthraquinones, terpenoids, alkaloids, polysaccharides, demonstrate therapeutic effects CVDs. TCM address CVD mechanisms, properties, antiinflammatory effects, enhancement function, prevention cell death (including apoptosis, autophagy, ferroptosis), modulation Clinical trials safety reducing symptoms Studies indicate numerous monomers sugar early renal via "gut-liver-kidney axis" while mitigating pathological kidneys. Xu exhibits protective influence kidneys interaction farnesoid X receptor (FXR). TCMs modulate microbiota-bile (BA) thereby slowing progression DKD. distinctive benefits kidney tissue damage, managing glucose lowering urinary protein levels, determined. DKD, there reduction supplementation these elements restore mucosa, reverse structural individuals TCM, combination Salvia miltiorrhiza Astragalus membranaceus effective prescription DKD (23). A. S. enhanced prevalence Akkermansia, muciniphila, Lactobacillus, L. murinus. While Akkermansia muciniphila exhibited synthesis arachidonic metabolites, Lactobacillus murinus exhibit glycerophospholipid suggesting may contribute positively flora distribution. Another astragaloside IV (AS-IV), active component astragaloside, facilitates release Nrf2 Kelch-like ECHassociated 1 (Keap1)-Nrf2 complex counteracts dysfunction (24). Huang-Lian-Jie-Du decoction, formulation, has improve metabolism rats advanced glycation endproducts (AGE)/ AGE (RAGE)/ kinase B (Akt)/Nrf2 triglyceride low-density lipoprotein cholesterol safeguard (25). Mycobacterial species possess powerful immunomodulatory capability, primarily due wall composition. Abdallah explore prophylactic anti-diabetic heat-killed Mycobacterium aurum (HK-MA) mice streptozotocin (STZ)-induced diabetes. Prophylactic administration three doses HK-MA diabetic led notable decrease compared control mice. notably normalized altered expression liver uncoupling 2 (UCP2), lactate dehydrogenase, skeletal muscle UCP3. lower hyperglycemia support supplement improving diabetes management. might be partially linked effect brought about HK-MA. However, still hypothesis necessitates additional research. contrast, Shi microbiome tumorpromoting anti-tumor effects. They outlined bacterial strategies tumors, encompassing engineered approaches. conditions, particular anaerobes parthenogenetic anaerobes, accumulate proliferate within tumor environment. occurrence triggers series responses body eventually lead metabolic by-products, induction cascades, responses, alteration microbial abundance colonization sites. metabolite indole-3-propionic aryl hydrocarbon (AhR) agonist. It reduces colonic cells, consequently hindering carcinogenesis anticancer Bifidobacterium classified advantages CRC. produces metabolites proliferation regulating suppressing important highlight butyrate, recognized most thoroughly researched short-chain fatty acids (SCFAs), role, possessing promote tumorigenesis elicit Butyrate demonstrates combat Fas p21 models pro-carcinogenic activities intestine, histone deacetylases. These actions inhibition further progression. Furthermore, butyrate preserving integrity functioning intestines. bidirectional two-sample Mendelian randomization (BTS-MR) Liu illustrated intricate thyroid dysfunction. underlines selecting more targeted uphold thyroid-gut homeostasis, prevention, management, reversal development. BTS-MR genera Intestinimonas, Eubacterium brachy group, Ruminiclostridium, Ruminococcaceae UCG004 risk decreased function. Lachnospiraceae UCG008, phyla Actinobacteria Verrucomicrobia, effects.Exploring products (26,27) production therapeutics molecular farming (28) garnered interest recent years reduced adverse medications. Considering current scientific evidence, it seems forthcoming incorporate antioxidants alongside conventional medical approach humans animals suffering necessary before utilized practice.

Language: Английский

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Protective effects of exogenous melatonin therapy against oxidative stress to male reproductive tissue caused by anti-cancer chemical and radiation therapy: a systematic review and meta-analysis of animal studies

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 28, 2023

Background Male testicular dysfunction is a considerable complication of anti-cancer therapies, including chemotherapy and radiotherapy, partly due to the increased oxidative stress caused by these treatments. Melatonin an effective antioxidant agent that protects testicles against physical toxic chemical stressors in animal models. This study aims systematically review melatonin’s protective effects on rodential tissue. Materials Method An extensive search was conducted Web Science, Scopus, PubMed for studies investigating exogenous rodent exposed chemicals radiotherapeutic agents. Using DerSimonian Laird random-effect model, standardized mean differences 95% confidence intervals were estimated from pooled data. The protocol prospectively registered International Prospective Register Systematic Reviews (PROSPERO: CRD42022355293). Results meta-analysis included 38 43 eligible review. Rats mice radiotherapy (ionizing radiations such as gamma- roentgen radiation radioactive iodine) or (methotrexate, paclitaxel, busulfan, cisplatin, doxorubicin, vinblastine, bleomycin, cyclophosphamide, etoposide, Taxol, procarbazine, docetaxel, chlorambucil). According our meta-analysis, all outcomes significantly improved melatonin therapy, sperm quantity quality (count, motility, viability, normal morphology, number spermatogonia, Johnsen’s biopsy score, seminiferous tubular diameter, epithelial height), serum level reproductive hormones (Follicle-Stimulating Hormone testosterone), tissue markers (testicular malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, glutathione, caspase-3, total capacity), weight-related characteristics (absolute body, epididymis, testis, relative testis body weights). Most SYRCLE domains exhibited high risk bias studies. Also, significant heterogeneity small-study detected. Conclusion In male rodents, therapy related histopathology, hormones, weights, reduced levels tissues rodents. Future meticulous are recommended provide robust scientific backbone human applications. registration https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022355293 , identifier CRD42022355293.

Language: Английский

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Impact of polyethylene microplastics exposure on kallikrein-3 levels, steroidal-thyroidal hormones, and antioxidant status in murine model: protective potentials of naringin

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 10, 2024

The widespread presence of microplastics in the environment has raised significant concerns regarding their potential impact on human and animal health. Among various microplastic types, polyethylene (PE-MPs) are particularly prevalent due to extensive use packaging consumer products. Exploring uncharted therapeutic potentials naringin, this study delves into its mitigating effects disruptions kallikrein-3 levels, steroidal-thyroidal hormone balance, antioxidant defense triggered by PE-MPs exposure, paving way for novel interventions environmental toxin-induced endocrine oxidative stress disorders. Male Wistar rats (n = 24) were randomly grouped four: Control, (1.5 mg/kg), + NAR mg/kg 100 NAR), (100 mg/kg). Hormonal parameters assessed after 28 days exposure. exposure caused a increase(p < 0.005) level (KLK-3) while it significantly reduces levels testosterone (TST), luteinizing hormone, thyroid stimulating (TSH) Free-triiodothyronine (fT3) Total cholesterol (TChol) concentration. also disrupted (p profile down-regulating activities glutathione-S-transferase, catalase (CAT), superoxide dismutase (SOD) reducing glutathione (GSH) ascorbic acid (AA) concentration malondialdehyde (MDA) increased relative control. However, naringin hormonal profiles demonstrated, as normalized KLK-3, steroid, concentration, enhanced defense. This suggests that is promising protective agent against damage induced contaminants such microplastics.

Language: Английский

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Is beta-carotene consumption associated with thyroid hormone levels?

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: May 26, 2023

The thyroid hormones play a pivotal role in various physiological processes, including growth, metabolism regulation, and reproduction. While non-modifiable factors are known to impact function, such as genetics age, nutritional also important. Diets rich selenium iodine conventionally acknowledged be beneficial for the production release of hormones. Recent studies have suggested potential link between beta-carotene, precursor vitamin A (retinol), function. Beta-carotene is its antioxidant properties has been shown prevention clinical conditions cancer cardiovascular neurological diseases. However, on function still unclear. Some positive association beta-carotene levels while others found no significant effect. Conversely, hormone produced by gland, thyroxine, enhances conversion retinol. Furthermore, derivatives being explored therapeutic options malignancies. In this review, we highlight mechanisms through which beta-carotene/retinol interact review findings examining consumption levels. Our underscores need further research clarify relationship

Language: Английский

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Inhibitory effects of progesterone on the human acute lymphoblastic leukemia cell line

Gene Reports, Journal Year: 2024, Volume and Issue: 36, P. 101991 - 101991

Published: July 26, 2024

Language: Английский

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Cisplatin-Induced Metabolic Responses Measured with Raman Spectroscopy in Cancer Cells, Spheroids, and Canine-Derived Organoids

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(38), P. 50267 - 50281

Published: Sept. 16, 2024

assessment of drug response with conventional cell viability assays remains the standard practice for guiding initial therapeutic choices. However, such ensemble approaches fail to capture heterogeneities in treatment and cannot identify early markers response. Here, we leverage Raman spectroscopy (RS) as an accurate, low-cost, extraction-free, label-free approach track metabolic changes cancer cells, spheroids, organoids cisplatin treatment. We identified 12 statistically significant metabolites cells 19 spheroids a function depth. show that 4T1 results shift metabolite levels; including nucleic acids DNA, 783 cm

Language: Английский

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