Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(49), P. 19605 - 19614
Published: Nov. 22, 2024
The
microsomal
epoxide
hydrolase
(mEH)
is
important
in
the
detoxification
of
carcinogens
liver
and
other
tissues
but
also
a
blood
biomarker
hepatitis
cancer.
Improved
analytical
methods
are
needed
for
study
its
role
metabolism
xenobiotics
endogenous
roles
as
diseases.
development
double
nanobody
sandwich
ELISA
offers
significant
improvements
over
traditional
polyclonal
or
monoclonal
antibody-based
assays,
enhancing
both
homogeneity
stability
assay
production.
This
focuses
on
selecting
optimizing
pairs
detecting
human
mEH.
Four
high-affinity
nanobodies
were
identified
tested
thermal
stability.
Combinations
these
evaluated,
revealing
that
MQ4-MQ30
pair
achieved
best
performance
with
limit
detection
(LOD)
1
ng/mL.
Additionally,
polyHRP
was
employed
signal
amplification,
capabilities
despite
challenges
related
to
small
size
single
epitope
recognition
nanobodies.
Comparative
studies
using
microplates
NHS@MF
membranes
performed.
superior
highlighted
their
potential
promising
alternative
point-of-care
testing.
exhibited
high
specificity
mEH
minimal
cross-reactivity
enzymes
effectively
addressed
matrix
effects
plasma
tissue
samples.
These
findings
underscore
ELISAs
reliable
sensitive
detection.
Journal of Nanobiotechnology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Feb. 6, 2025
Nanobodies
(Nbs),
miniature
antibodies
consisting
solely
of
the
variable
region
heavy
chains,
exhibit
unique
properties
such
as
small
size,
high
stability,
and
strong
specificity,
making
them
highly
promising
for
disease
diagnosis
treatment.
The
engineering
production
Nbs
has
evolved
into
a
mature
process,
involving
library
construction,
screening,
expression
purification.
Different
types,
including
immune,
naïve,
synthetic/semi-synthetic
libraries,
offer
diverse
options
various
applications,
while
display
platforms
like
phage
display,
cell
surface
non-surface
provide
efficient
screening
target
Nbs.
Recent
advancements
in
artificial
intelligence
(AI)
have
opened
new
avenues
Nb
engineering.
AI's
exceptional
performance
protein
structure
prediction
molecular
interaction
simulation
introduced
novel
perspectives
tools
design
optimization.
Integrating
AI
with
traditional
experimental
methods
is
anticipated
to
enhance
efficiency
precision
development,
expediting
transition
from
basic
research
clinical
applications.
This
review
comprehensively
examines
latest
progress
engineering,
emphasizing
construction
strategies,
platform
technologies,
It
evaluates
strengths
weaknesses
libraries
explores
potential
challenges
predicting
structure,
antigen-antibody
interactions,
optimizing
physicochemical
properties.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(45), P. 31174 - 31187
Published: Nov. 1, 2024
Multiple
and
ultrasensitive
detection
of
pathogenic
bacteria
is
critical
but
remains
a
challenge.
Here,
we
introduce
digital
assay
for
multiplexed
target
DNA
amplification-free
using
botryoidal-like
fluorescent
polystyrene
dots
(PS-dots),
which
were
first
prepared
through
the
hybridization
reaction
between
primer
exchange
chains
nanospheres
that
encapsulated
polymer
signal
preamplification.
The
bacteria's
was
cleavaged
by
argonaute
(Ago)
protein-mediated
multiple
precise
cleavage
reactions,
where
obtained
sequences
bridged
magnetic
beads
(MBs)
PS-dots
via
reaction,
MB-botryoidal
PS-dot
complexes
utilized
as
probes
based
on
colors
sizes
encoding.
An
artificial-intelligence-fluorescent
microsphere
counting
algorithm
applied
to
identify
count
MBs
readout.
This
combined
ultrabright
with
TrAC Trends in Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
176, P. 117770 - 117770
Published: May 17, 2024
Paper-based
analytical
devices
(PADs)
enable
the
affordable,
easy,
rapid,
and
reliable
detection
of
a
range
analytes
at
point-of-care.
Cellulose
is
versatile
substrate
for
antibody
attachment.
However,
how
immunoreagents
are
immobilized
onto
cellulose
plays
an
important
role
in
assay
performance.
To
provide
overview
different
immobilization
strategies
used
developing
PADs,
we
here
critically
review
existing
literature
from
last
decade
(2013-2023).
First,
introduce
as
summarize
fabrication
techniques
PADs.
Thereafter,
delve
into
antibodies
passively,
covalently,
or
by
affinity
linkage.
Affinity-based
further
explored
case
study
focused
on
using
recombinant
bifunctional
proteins
with
cellulose-binding
modules
to
anchor
cellulose.
The
final
section
discusses
validation
sustainable
development
PADs
addressing
future
challenges
associated
such
devices.
