The decisive early phase of bone regeneration

Nature Reviews Rheumatology, Journal Year: 2023, Volume and Issue: 19(2), P. 78 - 95

Published: Jan. 9, 2023

Language: Английский

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Electrical stimulation of piezoelectric BaTiO3 coated Ti6Al4V scaffolds promotes anti-inflammatory polarization of macrophages and bone repair via MAPK/JNK inhibition and OXPHOS activation

Biomaterials, Journal Year: 2022, Volume and Issue: 293, P. 121990 - 121990

Published: Dec. 26, 2022

Language: Английский

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Regulation of Osteoimmune Microenvironment and Osteogenesis by 3D‐Printed PLAG/black Phosphorus Scaffolds for Bone Regeneration

Advanced Science, Journal Year: 2023, Volume and Issue: 10(28)

Published: Aug. 24, 2023

The treatment of bone defects remains a significant challenge to be solved clinically. Immunomodulatory properties orthopedic biomaterials have significance in regulating osteoimmune microenvironment for osteogenesis. A lactic acid-co-glycolic acid (PLGA) scaffold incorporates black phosphorus (BP) fabricated by 3D printing technology investigate the effect BP on osteoimmunomodulation and osteogenesis site. PLGA/BP exhibits suitable biocompatibility, biodegradability, mechanical as an excellent support new formation. studies' result also demonstrate that scaffolds are able recruit stimulate macrophages M2 polarization, inhibit inflammation, promote human marrow mesenchymal stem cells (hBMSCs) proliferation differentiation, which turn promotes regeneration distal femoral defect region steroid-associated osteonecrosis (SAON) rat model. Moreover, it is screened demonstrated can osteogenic differentiation transcriptomic analysis, mineralization activating PI3K-AKT signaling pathway hBMSC cells. In this study, shown innovative extremely effective stimulating macrophage polarization strategy development used repair offered.

Language: Английский

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Recent Advances in 3D Printing of Smart Scaffolds for Bone Tissue Engineering and Regeneration

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(34)

Published: June 11, 2024

The repair and functional reconstruction of bone defects resulting from severe trauma, surgical resection, degenerative disease, congenital malformation pose significant clinical challenges. Bone tissue engineering (BTE) holds immense potential in treating these defects, without incurring prevalent complications associated with conventional autologous or allogeneic grafts. 3D printing technology enables control over architectural structures at multiple length scales has been extensively employed to process biomimetic scaffolds for BTE. In contrast inert grafts, next-generation smart possess a remarkable ability mimic the dynamic nature native extracellular matrix (ECM), thereby facilitating regeneration. Additionally, they can generate tailored controllable therapeutic effects, such as antibacterial antitumor properties, response exogenous and/or endogenous stimuli. This review provides comprehensive assessment progress 3D-printed BTE applications. It begins an introduction physiology, followed by overview technologies utilized scaffolds. Notable advances various stimuli-responsive strategies, efficacy, applications are discussed. Finally, highlights existing challenges development implementation scaffolds, well emerging this field.

Language: Английский

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Macrophage Polarization and the Regulation of Bone Immunity in Bone Homeostasis

Journal of Inflammation Research, Journal Year: 2023, Volume and Issue: Volume 16, P. 3563 - 3580

Published: Aug. 1, 2023

Abstract: Bone homeostasis is a dynamic equilibrium state of bone formation and absorption, ensuring skeletal development repair. immunity encompasses all aspects the intersection between immune systems, including various signaling pathways, cytokines, crosstalk cells under both homeostatic pathological conditions. Therefore, as key cell types in immunity, macrophages can polarize into classical pro-inflammatory M1 alternative anti-inflammatory M2 influence body environment, participating regulation metabolism playing roles homeostasis. not only act precursors osteoclasts (OCs), differentiate mature OCs, but also secrete cytokines to promote resorption; while osteogenic factors, stimulating differentiation mineralization osteoblast mesenchymal stem (MSCs), subsequently increase formation. Once polarization imbalanced, resulting dysregulation will cause inflammatory stimulation, release large amount factors affecting metabolism, leading conditions such osteoporosis (OP), rheumatoid arthritis (RA), steroid-induced femoral head necrosis (SANFH). In this review, we introduce pathways related macrophage polarization, well their relationships with OB, OC, MSC. We discuss diseases imbalance, regulating them, which may help develop new methods for treating metabolic disorders. Keywords: macrophage,

Language: Английский

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Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: March 20, 2023

Abstract Background Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation. Macrophages in the microenvironment are instrumental bone metabolism. Amount evidence have revealed exosomes transmitting lncRNA crucial nanocarriers for cellular interactions various biotic procedures, especially, osteogenesis. However, underlying mechanisms of regulatory relationship macrophages awaiting clarification. In present time study, we aimed to explore roles human umbilical vein endothelial cells (HUVECs)-derived carrying nuclear enrichment enriched transcript 1 (NEAT1) mediated by M2 polarized elucidate mechanisms. Results We demonstrated HUVECs-derived expressing NEAT1 significantly enhanced polarization attenuated LPS-induced inflammation vitro . Besides, conditioned medium from induced indirectly facilitated migration osteogenic differentiation marrow-derived mesenchymal stem (BMSCs). Mechanically, Exos decreased remarkably both expression dead-box helicase 3X-linked (DDX3X) nod-like receptor protein 3 (NLRP3). The level NLRP3 increased after RAW264.7 transfected with DDX3X overexpression plasmid. Additionally, knockdown partially counteracted aforementioned effect Exos. results air pouch rat model anti-inflammatory cytokines (IL-10) pro-inflammatory (IL-1β IL-6) vivo, contributing amelioration Afterwards, further confirmed encapsulated alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote regeneration, facilitate angiogenesis, increase infiltration as well decrease calvarial defect model. Conclusions enable alleviate inducing through DDX3X/NLRP3 axis, which finally contributes aid alginate/GelMA IPN vivo. Thus, our study provides insights healing exosomes-encapsulated composite hydrogels, exhibited potential towards use tissue engineering foreseeable future.

Language: Английский

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Macrophage plasticity: signaling pathways, tissue repair, and regeneration

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: Aug. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Language: Английский

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Macrophage polarization in the tumor microenvironment: Emerging roles and therapeutic potentials

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 116930 - 116930

Published: June 14, 2024

The tumor microenvironment (TME) is a combination of cells and indigenous host stroma, which consists tumor-infiltrating immune cells, endothelial fibroblasts, pericytes, non-cellular elements. Tumor-associated macrophages (TAMs) represent the major cell type are generally polarized into two functionally contradictory subtypes, namely classical activated M1 alternatively M2 macrophages. Macrophage polarization refers to how at given time space. interplay between TME macrophage can influence initiation progression, making TAM potential target for cancer therapy. Here, we review latest investigations on factors orchestrating in TME, affects perspectives modulating immunotherapy.

Language: Английский

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Advances in electroactive biomaterials: Through the lens of electrical stimulation promoting bone regeneration strategy

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 47, P. 191 - 206

Published: June 27, 2024

The regenerative capacity of bone is indispensable for growth, given that accidental injury almost inevitable. Bone relevant the aging population globally and repair large defects after osteotomy (e.g., following removal malignant tumours). Among many therapeutic modalities proposed to regeneration, electrical stimulation has attracted significant attention owing its economic convenience exceptional curative effects, various electroactive biomaterials have emerged. This review summarizes current knowledge progress regarding strategies improving repair. Such range from traditional methods delivering via electroconductive materials using external power sources self-powered biomaterials, such as piezoelectric nanogenerators. Electrical osteogenesis are related piezoelectricity. examines cell behaviour potential mechanisms electrostimulation in healing, aiming provide new insights regeneration biomaterials. roles rehabilitating microenvironment facilitate addressing whereby cues mediate regeneration. Interactions between osteogenesis-related cells summarized, leading proposals use stimulation-based therapies accelerate healing.

Language: Английский

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Current Perspectives of Protein in Bone Tissue Engineering: Bone Structure, Ideal Scaffolds, Fabrication Techniques, Applications, Scopes, and Future Advances

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(8), P. 5082 - 5106

Published: July 15, 2024

In view of their exceptional approach, excellent inherent biocompatibility and biodegradability properties, interaction with the local extracellular matrix, protein-based polymers have received attention in bone tissue engineering, which is a multidisciplinary field that repairs regenerates fractured bones. Bone multihierarchical complex structure, it performs several essential biofunctions, including maintaining mineral balance structural support protecting soft organs. Protein-based gained interest developing ideal scaffolds as emerging biomaterials for healing regeneration, challenging to design substitutes perfect biomaterials. Several polymers, collagen, keratin, gelatin, serum albumin, etc., are potential materials due cytocompatibility, controlled biodegradability, high biofunctionalization, tunable mechanical characteristics. While numerous studies indicated encouraging possibilities proteins BTE, there still major challenges concerning stability physiological conditions, continuous release growth factors bioactive molecules. Robust derived from can be used replace broken or diseased biocompatible substitute; proteins, being biopolymers, provide engineering. Herein, recent developments protein cutting-edge engineering addressed this review within 3–5 years, focus on significant future perspectives. The first section discusses fundamentals anatomy scaffolds, second describes fabrication techniques scaffolds. third highlights importance applications BTE. Hence, development state-of-the-art has been discussed, highlighting

Language: Английский

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