Ageing Research Reviews,
Journal Year:
2023,
Volume and Issue:
92, P. 102094 - 102094
Published: Oct. 18, 2023
As
a
global
public
health
problem,
low
back
pain
(LBP)
caused
by
intervertebral
disc
degeneration
(IDD)
seriously
affects
patients'
quality
of
life.
In
addition,
the
prevalence
IDD
tends
to
be
younger,
which
brings
huge
burden
individuals
and
society
economically.
Current
treatments
do
not
delay
or
reverse
progression
IDD.
The
emergence
biologic
therapies
has
brought
new
hope
for
treatment
Among
them,
extracellular
vesicles
(EVs),
as
nanoscale
bioactive
substances
that
mediate
cellular
communication,
have
now
produced
many
surprising
results
in
research
This
article
reviews
mechanisms
roles
EVs
delaying
describes
prospects
challenges
EVs.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(3), P. 2085 - 2085
Published: Jan. 20, 2023
Mesenchymal
stem
cells
(MSCs),
as
a
kind
of
pluripotent
cells,
have
attracted
much
attention
in
orthopedic
diseases,
geriatric
metabolic
and
sports
functions
due
to
their
osteogenic
potential,
chondrogenic
differentiation
ability,
adipocyte
differentiation.
Anti-inflammation,
anti-fibrosis,
angiogenesis
promotion,
neurogenesis,
immune
regulation,
secreted
growth
factors,
proteases,
hormones,
cytokines,
chemokines
MSCs
been
widely
studied
liver
kidney
cardiovascular
cerebrovascular
diseases.
In
recent
years,
many
studies
shown
that
the
extracellular
vesicles
similar
transplantation
all
above
aspects.
Here
we
review
research
progress
exocrine
years.
Biomaterials Research,
Journal Year:
2024,
Volume and Issue:
28
Published: Jan. 1, 2024
Intervertebral
discs
(IVDs)
have
a
limited
self-regenerative
capacity
and
current
strategies
for
IVD
regeneration
are
unsatisfactory.
Recent
studies
showed
that
small
extracellular
vesicles
derived
from
M2
macrophage
cells
(M2-sEVs)
inhibited
inflammation
by
delivery
of
various
bioactive
molecules
to
recipient
cells,
which
indicated
M2-sEVs
may
offer
therapeutic
strategy
the
repair
IVDs.
Herein,
we
investigated
roles
mechanisms
on
regeneration.
The
in
vitro
results
demonstrated
pyroptosis,
preserved
cellular
viability,
promoted
migration
nucleus
pulposus
(NPCs).
Bioinformatics
analysis
verification
experiments
microRNA
(miR)
expression
miR-221-3p
was
highly
expressed
M2-sEVs.
mechanism
action
explored
pyroptosis
NPCs
through
transfer
miR-221-3p,
suppressed
levels
phosphatase
tensin
homolog
NOD-,
LRR-,
pyrin
domain-containing
protein
3.
Moreover,
fabricated
decellularized
ECM-hydrogel
(dECM)
sustained
release
M2-sEVs,
exhibited
biocompatibility
controlled
properties.
vivo
revealed
dECM-hydrogel
containing
(dECM/M2-sEVs)
delayed
degeneration
intervertebral
disc
(IDD)
models.
In
addition
demonstrating
promising
IDD,
this
study
provided
valuable
data
furthering
understanding
Advanced Functional Materials,
Journal Year:
2022,
Volume and Issue:
32(52)
Published: Oct. 4, 2022
Abstract
Intervertebral
disc
(IVD)
degeneration
is
the
leading
cause
of
low
back
pain,
which
represents
a
highly
prevalent
chronic
aging‐associated
disorder
with
large
socio‐economic
burden
and
great
decline
in
quality
life,
contributing
to
pressing
need
for
IVD
treatments.
Thus,
finding
effective
disease‐modifying
approaches
postponing
progression
repairing
degenerated
intervertebral
discs
importance,
even
facing
various
challengeable
obstacles.
Bioactive
functional
materials
exhibit
excellent
advances
modulating
cell
activity
remodeling
tissue
homeostasis,
acts
as
promising
accessible
therapeutic
approach
repair
organ
reconstruction.
Recently,
these
have
been
notably
explored
investigate
bioactive
materials‐based
strategies
recovery
regeneration,
including
cell‐based
engineering,
development
implants
recovery,
methods
integrity
IVD.
This
review
summarizes
advanced
progress
discusses
their
regulatory
mechanisms,
limitations,
challenges
well
clinical
translational
prospects
future.
Biological Procedures Online,
Journal Year:
2024,
Volume and Issue:
26(1)
Published: May 21, 2024
Extracellular
vesicles
(EVs)
are
nano-sized,
membranous
transporters
of
various
active
biomolecules
with
inflicting
phenotypic
capabilities,
that
naturally
secreted
by
almost
all
cells
a
promising
vantage
point
as
potential
leading
drug
delivery
platform.
The
intrinsic
characteristics
their
low
toxicity,
superior
structural
stability,
and
cargo
loading
capacity
continue
to
fuel
multitude
research
avenues
dedicated
EVs
therapeutic
diagnostic
cargos
(pharmaceutical
compounds,
nucleic
acids,
proteins,
nanomaterials)
in
attempts
generate
natural
nanoscale
systems
for
clinical
application
therapeutics.
In
addition
well-known
role
intercellular
communication,
harbor
microRNAs
(miRNAs),
which
can
alter
the
translational
receiving
thus
act
important
mediators
numerous
biological
pathological
processes.
To
leverage
this
potential,
be
structurally
engineered
shuttle
miRNAs
diseased
recipient
targeted
'treatment'
or
'therapy'.
Herein,
review
focuses
on
EV-coupled
miRNAs;
summarizing
biogenesis,
contents,
function
EVs,
well
providing
both
comprehensive
discussion
current
EV
techniques
an
update
miRNA-engineered
next-generation
platform
piloting
benchtop
studies
propel
translation
forefront
nanomedicine.
Stem Cell Research & Therapy,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 4, 2025
Type1
Diabetes
(T1D)
is
an
autoimmune
disorder
characterised
by
the
loss
of
pancreatic
β-cells.
This
β
cell
occurs
primarily
through
inflammatory
pathways
culminating
in
apoptosis.
Mesenchymal
stromal
cells
(MSCs)
have
been
heavily
studied
for
therapeutic
applications
due
to
their
regenerative,
anti-apoptotic,
immunomodulatory,
and
anti-inflammatory
properties.
The
effects
MSCs
are
mediated
cell-to-cell
contact,
differentiation,
release
paracrine
factors,
which
include
extracellular
vesicles
(EVs).
Culturing
hypoxia,
a
low
oxygen
tension
state
more
analogous
physiological
environment,
seems
increase
efficacy
MSC
therapy,
enhancing
anti-inflammatory,
anti-fibrotic
also
case
with
MSC-derived
EVs,
show
altered
properties
based
on
parent
preconditioning.
In
this
review,
we
examine
evidence
supporting
potential
application
hypoxic
preconditioning
strengthening
MSC-EVs
treating
apoptotic
causes
T1D.
Cancer Treatment and Research Communications,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100924 - 100924
Published: April 1, 2025
Extracellular
vesicles
(EVs)
are
produced
by
virtually
all
types
of
cells
and
can
be
detected
in
nearly
extracellular
places.
These
particles
mediate
intercellular
communication
transfer
their
cargo
to
the
recipient
cells,
inducing
a
variety
processes
these
through
transmission
several
biomolecules
such
as
miRNAs,
lncRNAs,
other
transcripts
proteins.
It
has
been
documented
that
size,
quantity,
expression
EVs
influenced
level
oxygen.
In
fact,
hypoxia
affect
cellular
modulation
vesicles.
Hypoxic
exosomes
derived
from
tumor
have
protumoral
effects
on
including
enhancement
proliferation,
migration,
invasion
tumoral
induction
metastasis
distant
organs,
stimulation
angiogenesis
endothelial
macrophage
polarization.
also
contribute
non-malignant
diseases.
This
review
summarizes
effect
malignant
nonmalignant
diseases
different
organs.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: June 9, 2023
Degenerative
orthopedic
diseases,
as
a
global
public
health
problem,
have
made
serious
negative
impact
on
patients'
quality
of
life
and
socio-economic
burden.
Traditional
treatments,
including
chemical
drugs
surgical
obvious
side
effects
unsatisfactory
efficacy.
Therefore,
biological
therapy
has
become
the
focus
researches
degenerative
diseases.
Extracellular
vesicles
(EVs),
with
superior
properties
immunoregulatory,
growth
support,
drug
delivery
capabilities,
emerged
new
cell-free
strategy
for
treatment
many
An
increasing
number
studies
shown
that
EVs
can
be
engineered
through
cargo
loading,
surface
modification,
synthesis
to
improve
efficiency,
specificity,
safety.
Herein,
comprehensive
overview
recent
advances
in
engineering
strategies
applications
well
related
osteoarthritis
(OA),
osteoporosis
(OP),
intervertebral
disc
degeneration
(IDD)
osteonecrosis
femoral
head
(ONFH),
is
provided.
In
addition,
we
analyze
potential
challenges
applying
clinical
practice.
Biotechnology Letters,
Journal Year:
2024,
Volume and Issue:
46(2), P. 279 - 293
Published: Feb. 13, 2024
Abstract
Purpose
3D
cell
culture
and
hypoxia
have
been
demonstrated
to
increase
the
therapeutic
effects
of
mesenchymal
stem/stromal
cells
(MSCs)-derived
extracellular
vesicles
(EVs).
In
this
study,
a
process
for
production
MSC-EVs
in
novel
bioreactor
system
under
normoxic
hypoxic
conditions
was
established
resulting
EVs
were
characterized.
Methods
Human
adipose-derived
MSCs
seeded
cultured
on
membrane
VITVO®
7
days.
Afterwards,
isolated
characterized
via
fluorescence
nanoparticle
tracking
analysis,
flow
cytometry
with
staining
against
annexin
V
(Anx5)
as
marker
exposing
phosphatidylserine,
well
CD73
CD90
MSC
surface
markers.
Results
Cultivation
higher
concentration
from
(9.1
×
10
9
±
1.5
9.7
3.1
particles/mL)
compared
static
2D
(4.2
7.5
8
3.9
3.0
conditions,
respectively.
Also,
particle-to-protein
ratio
measure
purity
increased
3.3
1.1
particles/µg
protein
1.6
8.3
6
3D.
Total
−
+
elevated
conditions.
The
EV
size
did
not
differ
significantly
between
Conclusion
resulted
purity.
This
could
be
especially
useful
screening
3D-derived
MSC-EVs.
Drug Design Development and Therapy,
Journal Year:
2024,
Volume and Issue:
Volume 18, P. 1007 - 1022
Published: March 1, 2024
Abstract:
Low
back
pain
(LBP)
is
a
common
condition
that
has
substantial
consequences
on
individuals
and
society,
both
socially
economically.
The
primary
contributor
to
LBP
often
identified
as
intervertebral
disc
degeneration
(IVDD),
which
worsens
leads
significant
spinal
problems.
conventional
treatment
approach
for
IVDD
involves
physiotherapy,
drug
therapy
management,
and,
in
severe
cases,
surgery.
However,
none
of
these
treatments
address
the
underlying
cause
condition,
meaning
they
cannot
fundamentally
reverse
or
restore
mechanical
function
spine.
Nanotechnology
regenerative
medicine
have
made
advancements
field
healthcare,
particularly
area
nanodrug
delivery
systems
(NDDSs).
These
approaches
demonstrated
potential
enhancing
efficacy
by
providing
benefits
such
high
biocompatibility,
biodegradability,
precise
targeted
areas,
prolonged
release,
improved
therapeutic
results.
different
NDDSs
designed
delivering
various
genes,
cells,
proteins
drugs
opened
up
new
opportunities
effectively
addressing
IVDD.
This
comprehensive
review
provides
consolidated
overview
recent
use
It
emphasizes
overcoming
challenges
associated
with
this
condition.
Meanwhile,
insights
ideas
presented
aim
contribute
advancement
using
NDDSs.
Keywords:
lumbar
herniation,
degeneration,
nanocarrier
system,
therapy,
