Mechanical Stimulation and Aligned Poly(ε-caprolactone)–Gelatin Electrospun Scaffolds Promote Skeletal Muscle Regeneration DOI Creative Commons
Francisco José Calero-Castro, Víctor Manuel Pérez Puyana, Imán Laga

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(10), P. 6430 - 6440

Published: Oct. 4, 2024

The current treatments to restore skeletal muscle defects present several injuries. creation of scaffolds and implant that allow the regeneration this tissue is a solution reaching researchers' interest. To achieve this, electrospinning useful technique manufacture with nanofibers different orientation. In work, polycaprolactone gelatin solutions were tested fabricate electrospun two degrees alignment between their fibers: random aligned. These can be seeded myoblast C2C12 then stimulated mechanical bioreactor mimics physiological conditions tissue. Cell viability as well cytoskeletal morphology functionality was measured. Myotubes in aligned (9.84 ± 1.15 μm) thinner than (11.55 3.39 μm;

Language: Английский

Beyond hype: unveiling the Real challenges in clinical translation of 3D printed bone scaffolds and the fresh prospects of bioprinted organoids DOI Creative Commons
Xiangyu Zhao, Na Li, Ziqi Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 21, 2024

Bone defects pose significant challenges in healthcare, with over 2 million bone repair surgeries performed globally each year. As a burgeoning force the field of tissue engineering, 3D printing offers novel solutions to traditional transplantation procedures. However, current 3D-printed scaffolds still face three critical material selection, methods, cellular self-organization and co-culture, significantly impeding their clinical application. In this comprehensive review, we delve into performance criteria that ideal should possess, particular focus on core faced by technology during translation. We summarize latest advancements non-traditional materials advanced techniques, emphasizing importance integrating organ-like technologies bioprinting. This combined approach enables more precise simulation natural structure function. Our aim writing review is propose effective strategies address these promote translation for defect treatment.

Language: Английский

Citations

17
Developing functional hydrogels for treatment of oral diseases DOI Creative Commons
Chuanhui Song, Rui Liu, Yile Fang

et al.

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(3)

Published: July 25, 2024

Oral disease is a severe healthcare challenge that diminishes people's quality of life. Functional hydrogels with suitable biodegradability, biocompatibility, and tunable mechanical properties have attracted remarkable interest been developed for treating oral diseases. In this review, we present up-to-date research on the management dental caries, endodontics, periapical periodontitis, depending progression The strategies mucosal diseases salivary gland are then classified. After that, focus application related to tumor therapy tissue defects. Finally, review prospects restrictions perspectives utilization in treatment. We believe will promote advancement more amicable, functional personalized approaches

Language: Английский

Citations

5
Effects of Chrono-Exercise and Chrono-Nutrition on Muscle Health: Understanding the Molecular Mechanisms Activated by Timed Exercise and Consumption of Proteins and Carbohydrates DOI Creative Commons
Mariarosaria Negri,

Claudia Pivonello,

Feliciana Amatrudo

et al.

Nutrition Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The circadian clock is an endogenous timekeeping system that regulates various physiological and behavioral processes. Recently, it has been shown the timing of physical activity food intake can significantly influence metabolic muscle health. Some recent clinical evidence practiced in late afternoon be more effective terms performance strength. Preclinical studies have highlighted explanation for this effect lies different daily expression genes clock-controlled involved development hypertrophy. In conjunction with scientific advances understanding molecular mechanisms regulate rhythms trophy, chrono-nutrition gained resonance become a promising field, aimed at regulation body metabolism. Clinical preclinical protein consumption specific time points during day, or precisely after exercise, activate signaling pathways synthesis and, thus, favor skeletal mass as well mitochondrial biogenesis, thereby improving cell energy production function. On other hand, some carbohydrates immediately exercise increases insulin secretion, which facilitates glucose uptake by cells to replenish glycogen. This review summarizes current literature concerning chrono-exercise health, focusing on involving mass, strength, Understanding intricate relationship between rhythms, nutrition, metabolism essential optimizing nutritional strategies prevent treat wasting. addition, tailoring carbohydrate individual needs improve maintenance, growth, performance.

Language: Английский

Citations

0
Leveraging Large Language Models for Accelerated Learning and Innovation in Biogenic Tissue-Engineered Vascular Grafts DOI
Fernando Gomes de Souza,

Karine Velasco,

S. D. C. CUNHA

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106935 - 106935

Published: April 1, 2025

Language: Английский

Citations

0
Tissue Engineering and Regenerative Medicine: Perspectives and Challenges DOI Creative Commons
Van T. Hoang, Quyen Thi Nguyen,

Trang Thi Kieu Phan

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(5)

Published: April 24, 2025

ABSTRACT From the pioneering days of cell therapy to achievement bioprinting organs, tissue engineering, and regenerative medicine have seen tremendous technological advancements, offering solutions for restoring damaged tissues organs. However, only a few products technologies received United States Food Drug Administration approval. This review highlights significant progress in therapy, extracellular vesicle‐based engineering. Hematopoietic stem transplantation is powerful tool treating many diseases, especially hematological malignancies. Mesenchymal cells been extensively studied. The discovery induced pluripotent has revolutionized disease modeling applications, paving way personalized medicine. Gene represents an innovative approach treatment genetic disorders. Additionally, therapies emerged as rising stars, promising diagnostics, cell‐free therapeutics, drug delivery, targeted therapy. Advances engineering enable complex constructs, further transforming field. Despite these technical, ethical, regulatory challenges remain. addresses current bottlenecks, emphasizing novel interdisciplinary research overcome hurdles. Standardizing practices conducting clinical trials will balance innovation regulation, improving patient outcomes quality life.

Language: Английский

Citations

0
Bioprinting Taurine-Incorporated Gelatin Methacrylate Hydrogels for Enhanced Muscle Tissue Regeneration DOI
Nima Tabatabaei Rezaei, Kartikeya Dixit,

Sangeeta Shrivas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Abstract Skeletal muscle diseases like myopathies and muscular dystrophies present significant clinical challenges with few effective treatments. To better understand disease mechanisms accelerate therapy development, robust in vitro models are needed. Extrusion- light-based bioprinting offer precise fabrication of tissue-like constructs, but creating bioinks that support cell function remains difficult. Here, we report a novel bioink which taurine is first methacrylated—substituting its –NH 2 groups to afford methacrylate (TMA)—enabling covalent integration into gelatin methacryloyl (GelMA) networks. We systematically compared GelMA-Taur (physical blend) versus GelMA-TMA (covalent) hydrogels, assessing mechanical stiffness, swelling behavior, photocrosslinking kinetics. Incorporating TMA yielded tighter crosslinking control, minimized overcure DLP-printed features, improved shape fidelity. SEM revealed finer pore structures homogeneous distribution, release assays confirmed prolonged retention rapidly leaching taurine. Photopatterning 3D printing complex geometries demonstrated excellent printability the bioink. Finally, C2C12 myoblasts encapsulated scaffolds exhibited accelerated early differentiation, higher myosin heavy chain expression, more extensive myotube formation than controls. Together, these results establish as printable, mechanically tunable, biologically active platform for engineering skeletal tissue modeling regenerative applications.

Language: Английский

Citations

0
A comprehensive review of additively manufactured biomedical titanium-based alloys for bone tissue engineering: Biocorrosion, biomechanical, and biological properties DOI Creative Commons
Amir Hossein Noorbakhsh Nezhad, E. Mohammadi Zahrani,

Saba Mousavinasab

et al.

Journal of Materials Research and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
3D-bioprinted urethral grafts: Revolutionizing urethral stricture treatment DOI Creative Commons
Kirolos Eskandar

Arab Journal of Urology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: May 13, 2025

Language: Английский

Citations

0
Collagen patches releasing phosphatidylserine liposomes guide M1-to-M2 macrophage polarization and accelerate simultaneous bone and muscle healing DOI
Riki Toita, Yuki Shimizu, Eiko Shimizu

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 187, P. 51 - 65

Published: Aug. 17, 2024

Language: Английский

Citations

3
Mechanical Stimulation and Aligned Poly(ε-caprolactone)–Gelatin Electrospun Scaffolds Promote Skeletal Muscle Regeneration DOI Creative Commons
Francisco José Calero-Castro, Víctor Manuel Pérez Puyana, Imán Laga

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(10), P. 6430 - 6440

Published: Oct. 4, 2024

The current treatments to restore skeletal muscle defects present several injuries. creation of scaffolds and implant that allow the regeneration this tissue is a solution reaching researchers' interest. To achieve this, electrospinning useful technique manufacture with nanofibers different orientation. In work, polycaprolactone gelatin solutions were tested fabricate electrospun two degrees alignment between their fibers: random aligned. These can be seeded myoblast C2C12 then stimulated mechanical bioreactor mimics physiological conditions tissue. Cell viability as well cytoskeletal morphology functionality was measured. Myotubes in aligned (9.84 ± 1.15 μm) thinner than (11.55 3.39 μm;

Language: Английский

Citations

3