Modification with Conventional Surfactants to Improve a Lipid-Based Ionic-Liquid-Associated Transcutaneous Anticancer Vaccine DOI Creative Commons
Shihab Uddin, Rafiqul Islam, Rahman Md Moshikur

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(7), P. 2969 - 2969

Published: March 27, 2023

Transcutaneous vaccination is one of the successful, affordable, and patient-friendly advanced immunization approaches because presence multiple immune-responsive cell types in skin. However, absence a preferable facilitator, skin's outer layer strong impediment to delivering biologically active foreign particles. Lipid-based biocompatible ionic-liquid-mediated nanodrug carriers represent an expedient distinct strategy permit transdermal drug delivery; with acceptable surfactants, performance formulations might be further enhanced. For this purpose, we formulated lipid-based nanovaccine using conventional (cationic/anionic/nonionic) surfactant loaded antigenic protein immunomodulator its core promote delivery by penetrating skin boosting immunogenic activity. In follow-up investigation, freeze-dry emulsification process was used prepare nanovaccine, delivery, pharmacokinetic parameters, ability activate autoimmune cells tumor microenvironment were studied tumor-budding C57BL/6N mouse model. These analyses performed ELISA, nuclei HE staining, flow cytometry, other biological techniques. The immunomodulator-containing significantly (p < 0.001) increased anticancer immune responses (IgG, IgG1, IgG2, CD8+, CD207+, CD103+ expression) without causing cellular or toxicity. Using nanovaccination approach, it possible create more targeted efficient system for cancer antigens, thereby stimulating stronger response compared aqueous formulations. This lead effective therapeutic preventative outcomes patients cancer.

Language: Английский

Microneedle-Mediated Vaccination: Innovation and Translation DOI
Tao Sheng, Bowen Luo, Wentao Zhang

et al.

Advanced Drug Delivery Reviews, Journal Year: 2021, Volume and Issue: 179, P. 113919 - 113919

Published: Aug. 8, 2021

Language: Английский

Citations

115
Jet injectors: Perspectives for small volume delivery with lasers DOI Creative Commons
Jelle J. Schoppink, David Fernández Rivas

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 182, P. 114109 - 114109

Published: Jan. 6, 2022

Needle-free jet injectors have been proposed as an alternative to injections with hypodermic needles. Currently, a handful of commercial needle-free already exist. However, these are designed for specific injections, typically limited large injection volumes into the deeper layers beneath skin. There is growing evidence advantages when delivering small superficial skin layers, namely epidermis and dermis. Injections such vaccines insulin would benefit from delivery layers. Furthermore, same technology volume can serve (medical) tattooing well other personalized medicine treatments. The research dedicated actuated by laser energy has increased in last decade. In this case, absorption optical liquid results explosively bubble. This bubble displaces rest liquid, resulting fast microfluidic which penetrate technique allows precise control over (pL µL) penetration depths (µm mm). be tuned without changing device, varying parameters power, beam diameter filling level container. Despite published on working principles capabilities individual laser-actuated injectors, thorough overview encompassing all them lacking. perspective, we will discuss current status laser-based contrast their limitations, potential challenges.

Language: Английский

Citations

67
Mind the “Vaccine Fatigue” DOI Creative Commons
Zhaohui Su, Ali Cheshmehzangi, Dean McDonnell

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: March 10, 2022

Background Omicron scares and speculations are gaining momentum. Amid the nonstop debates discussions about COVID-19 vaccines, “vaccine fatigue” phenomenon may become more prevalent. However, to date, no research has systematically examined factors that shape people’s vaccine fatigue. To bridge gap, this study aims investigate antecedents cause or catalyze Methods A narrative literature review was conducted in PubMed, Scopus, PsycINFO identify The search completed on December 6, 2021, with a focus scholarly published English. Results total of 37 articles were reviewed analyzed. Vaccine fatigue most frequently discussed context infectious diseases general at pre-vaccination stage. been identified public, parents, doctors. Overall, wide range identified, ranging from frequency immunization demands, side effects, misconceptions severity need for vaccination, lack trust government media. Conclusion is inertia inaction towards information instruction due perceived burden burnout. Our found while some contributors rooted limitations sciences therefore can hardly be avoided, effective empathetic communications hold great promise eliminating preventable across sectors society.

Language: Английский

Citations

63
Toward closed-loop drug delivery: Integrating wearable technologies with transdermal drug delivery systems DOI
Jyothsna Manikkath,

J. Anand Subramony

Advanced Drug Delivery Reviews, Journal Year: 2021, Volume and Issue: 179, P. 113997 - 113997

Published: Oct. 9, 2021

Language: Английский

Citations

62
mRNA nanodelivery systems: targeting strategies and administration routes DOI Creative Commons
Mujie Yuan, Zeyu Han, Yan Liang

et al.

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

With the great success of coronavirus disease (COVID-19) messenger ribonucleic acid (mRNA) vaccines, mRNA therapeutics have gained significant momentum for prevention and treatment various refractory diseases. To function efficiently in vivo overcome clinical limitations, demands safe stable vectors a reasonable administration route, bypassing multiple biological barriers achieving organ-specific targeted delivery mRNA. Nanoparticle (NP)-based systems representing leading vector approaches ensure successful intracellular to target organ. In this review, chemical modifications types advanced NPs, including lipid NPs polymers are summarized. The importance passive targeting, especially endogenous active targeting nano-delivery is emphasized, different cellular endocytic mechanisms discussed. Most importantly, based on above content physiological structure characteristics organs vivo, design strategies cells classified Furthermore, influence routes highlighted. Finally, an outlook remaining challenges future development toward therapies precision medicine provided.

Language: Английский

Citations

32
Microneedle‐assisted transdermal delivery of nanoparticles: Recent insights and prospects DOI Creative Commons
Antonio José Guillot, Miquel Martínez‐Navarrete, Valeria Zinchuk‐Mironova

et al.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2023, Volume and Issue: 15(4)

Published: April 11, 2023

Abstract Transdermal delivery of drugs offers an interesting alternative for the administration molecules that present certain troubles when delivered by oral route. It can produce systemic effects or perform a local action formulation exerts optimal controlled drug release targeted to specific cell type site. also avoids several inconveniences such as hepatic first pass effect, gastric pH‐induced hydrolysis, malabsorption because diseases surgeries, and unpleasant organoleptic properties. Nanomedicine microneedle array patches (MAPs) are two trendiest systems applied transdermal research nowadays. However, skin is protective barrier nanoparticles (NPs) cannot through intact stratum corneum . The association NPs MAPs (NPs@MAPs) work synergistically, since assist bypass outer layers, contribute system providing delivery. Vaccination tailored therapies have been proposed fields where both great potential due inherent characteristics. conception easy use could allow self‐administration therefore facilitate mass vaccination campaigns in undeveloped areas with weak healthcare services. Additionally, nanomedicine being explored platform personalize important field oncology. In this we show recent insights prove benefits NPs@MAPs analyze prospects discrete interest industry NPs@MAPs, evaluating different limiting steps restricts translation clinical practice. This article categorized under: Nanotechnology Approaches Biology > NA Therapeutic Drug Discovery

Language: Английский

Citations

27
Beyond the Needle: Innovative Microneedle-Based Transdermal Vaccination DOI Creative Commons
Hiep X. Nguyen

Medicines, Journal Year: 2025, Volume and Issue: 12(1), P. 4 - 4

Published: Feb. 7, 2025

Vaccination represents a critical preventive strategy in the current global healthcare system, serving as an indispensable intervention against diverse pathogenic threats. Although conventional immunization relies predominantly on hypodermic needle-based administration, this method carries substantial limitations, including needle-associated fear, bloodborne pathogen transmission risks, occupational injuries among workers, waste management issues, and dependence trained medical personnel. Microneedle technology has emerged innovative vaccine delivery offering convenient, effective, minimally invasive administration. These microscale needle devices facilitate targeted antigen to epidermal dermal tissues, where abundant populations of antigen-presenting cells, specifically Langerhans dendritic provide robust immunological responses. Multiple research groups have extensively investigated microneedle-based vaccination strategies. This transdermal technique offers several advantages, notably circumventing cold-chain requirements enabling self-administration. Numerous preclinical investigations clinical trials demonstrated safety profile, immunogenicity, patient acceptance microneedle-mediated across applications. comprehensive review examines fundamental aspects immunization, principles, transcutaneous strategies, delivery-including classifications, barriers. Furthermore, addresses technical considerations, such treatment efficacy, application methodologies, wear duration, dimensional optimization, manufacturing processes, regulatory frameworks, sustainability followed by analysis future perspective technology.

Language: Английский

Citations

1
Enhancing vaccine stability in transdermal microneedle platforms DOI
Suman Pahal,

Feifei Huang,

Parbeen Singh

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

Citations

1
Roles of Lipids in the Permeability Barriers of Skin and Oral Mucosa DOI Open Access
Philip W. Wertz

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(10), P. 5229 - 5229

Published: May 15, 2021

PubMed searches reveal much literature regarding lipids in barrier function of skin and less on the oral mucosa. In terrestrial mammals, birds, reptiles, skin’s permeability is provided by ceramides, fatty acids, cholesterol outermost layers epidermis, stratum corneum. This layer consists about 10–20 cornified cells embedded a lipid matrix. It effectively prevents loss water electrolytes from underlying tissue, it limits penetration potentially harmful substances environment. cavity, regions gingiva hard palate are covered keratinized epithelia that resemble epidermis. The corneum contains mixture similar to epidermal but lower amounts accordingly more permeable. superficial nonkeratinized also provide barrier. These epithelial do contain cholesterol, free which may underlie function. barriers primarily protect tissue preventing toxic substances, including microbial products. Transdermal drug delivery, buccal absorption, lipid-related disease discussed.

Language: Английский

Citations

52
A single subcutaneous or intranasal immunization with adenovirus‐based SARS‐CoV‐2 vaccine induces robust humoral and cellular immune responses in mice DOI Creative Commons
Eun Ran Kim, Florian Weisel, Stephen C. Balmert

et al.

European Journal of Immunology, Journal Year: 2021, Volume and Issue: 51(7), P. 1774 - 1784

Published: March 27, 2021

Optimal vaccines are needed for sustained suppression of SARS-CoV-2 and other novel coronaviruses. Here, we developed a recombinant type 5 adenovirus vector encoding the gene S1 subunit antigen (Ad5.SARS-CoV-2-S1) COVID-19 immunization evaluated its immunogenicity in mice. A single with Ad5.SARS-CoV-2-S1 via S.C. injection or I.N delivery induced robust antibody cellular immune responses. Vaccination elicited significant S1-specific IgG, IgG1, IgG2a endpoint titers as early 2 weeks, antibodies were long lasting. I.N. administration produced GC B cells cervical axillary LNs, respectively. Moreover, evoked significantly greater antigen-specific T-cell responses compared to unimmunized control groups indications that was more effective than eliciting Mice vaccinated by either route demonstrated increased virus-specific neutralization on weeks 8 12 groups, well BM forming (AFC), indicative long-term immunity. Thus, this Ad5-vectored vaccine candidate showed promising following mice routes administration, supporting further development Ad-based against infectious diseases sustainable global programs.

Language: Английский

Citations

41