High-throughput, label-free detection of DNA origami in single-cell suspensions using origamiFISH-Flow DOI Creative Commons

Shana Alexander,

Wendy Xueyi Wang, Chung‐Yi Tseng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 2, 2023

Abstract Structural DNA nanotechnology enables custom fabrication of nanoscale devices and promises diverse biological applications. However, the effects design on nanostructure (DN)-cell interactions in vitro vivo are not yet well-characterized. origamiFISH is a recently developed technique for imaging DNs cells tissues. Compared to use fluorescent tags, offers label-free structure-agnostic detection with significantly improved sensitivity. Here, we extend quantifying single-cell suspensions, including nonadherent such as subsets immune cells, via readout by flow cytometry. This method, referred origamiFISH-Flow, high-throughput (e.g., 10,000 per second) compatible immunostaining concurrent cell-type -state characterization. We demonstrate that origamiFISH-Flow enhances signal-to-noise ratio up 20-fold compared dye labeling approaches, leading capture >25-fold more DN + at low, single-picomolar uptake concentrations. additionally show profile shape-specific patterns across cell lines splenocytes quantify accumulation lymphoid organs. Together, new tool interrogate tissues, while providing insights tailoring their designs bio-applications.

Language: Английский

Breaking Barriers: Innovative Fabrication Processes for Nanostructured Materials and Nano Devices DOI Creative Commons
Kaushal Kumar, Saurav Dixit,

Md. Zia ul Haq

et al.

E3S Web of Conferences, Journal Year: 2023, Volume and Issue: 430, P. 01197 - 01197

Published: Jan. 1, 2023

This study explores the world of nanostructured materials and new production procedures, revealing how they interact to break down obstacles in research. We uncover confluence between theoretical goals actual reality by investigating manufacturing processes. The links processes material characteristics, establishing groundwork for informed applications electronics, photonics, sensors, biology. empirical journey illuminates problems opportunities, prompting joint efforts push these frontiers forward. article is a testimony materials' transformational potential, challenging researchers dreamers redefine technological landscapes using their unique features inventive production.

Language: Английский

Citations

69
Nanoparticles derived from herbal preparations May represent a novel nucleic acid therapy DOI Creative Commons
T Feng, Waqas Ahmed, Talha Ahmed

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(2)

Published: Feb. 2, 2024

Abstract A significant number of nucleic acid therapies have been approved, highlighting the vast potential therapeutics. Although numerous delivery strategies investigated, and progress has made in systems, oral route still presents a obstacle. Recently, novel promising force emerged, namely Decoctosomes Bencaosomes, obtained from decoction natural herbs, which refers to boiled Chinese herbal medicines. The active components include small molecules, peptides, RNAs (sRNAs). sRNAs were first targets extensive research. Additionally, Bencaosomes proven effective several disease mouse models as artificially enhanced decoctosome products. This perspective summarizes development possible future directions two varieties nanoparticles that may open door new possibilities therapy.

Language: Английский

Citations

7
RNA-based therapies in liver metabolic diseases DOI Creative Commons
Antonio Fontanellas, Pedro Berraondo, F Urigo

et al.

Gut, Journal Year: 2025, Volume and Issue: unknown, P. gutjnl - 331742

Published: Feb. 23, 2025

RNA-based therapeutics have rapidly emerged over the past decade, offering a new class of medicines that differ significantly from conventional drugs. These therapies can be programmed to target or restore defective genes, allowing for more personalised treatments and reducing side effects. Notably, RNA made significant progress in treatment genetic liver diseases, exemplified by small interfering hereditary transthyretin amyloidosis, which use liver-targeting strategies such as GalNAc conjugation improve efficacy safety. gene-editing technologies, base editor prime clustered regularly interspaced short palindromic repeats systems, also show promise with their ability minimise genomic rearrangements cancer risk. While offer high precision, challenges remain optimising delivery methods ensuring long-term safety efficacy. Lipid nanoparticle-mRNA therapeutics, particularly protein replacement rare gained support preclinical successes. Compared viral gene therapies, mRNA present safer profile reduced risks integration oncogene activation. However, clinical trials, especially face limitations sample sizes observation periods. Further studies, including non-human primates, will essential refining trial designs. Despite potential, costs pose challenge require cost–utility models guide pricing accessibility. Here, we discuss fundamental aspects showcase most relevant developments metabolic diseases.

Language: Английский

Citations

0
Evaluation of Pharmacokinetics, Immunogenicity, and Immunotoxicity of DNA Tetrahedral and DNA Polymeric Nanostructures DOI
Yanfei Guo, Yan Huang, Mingxing Liu

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Deoxyribonucleic acid (DNA) nanostructures have been extensively explored in biomedical applications and emerged as a promising platform for drug delivery, bioanalysis, therapeutics. Their vivo behaviors received much attention, prerequisite clinical applications. Herein, the pharmacokinetics, immunogenicity, immunotoxicity of two representative DNA nanostructures: tetrahedron (TDN) nanoparticle (DNP) are studied. The pharmacokinetics monitored mouse model via tracking 32 P radiolabeled, half‐lives TDN DNP 9.88 19.80 min, respectively. preferentially accumulate liver kidney one half‐life metabolized through liver, kidney, excreta after 24 h. Meanwhile, elicit weak pro‐inflammatory immune response with low immunogenicity non‐immunotoxic, shown by evaluation, histopathology, serum biochemical index analysis. This research shows that safe biological systems, indicating can be developed therapeutic platforms biomedicine.

Language: Английский

Citations

3
In Vivo Interactions of Nucleic Acid Nanostructures With Cells DOI Creative Commons
Yu Xiao, Zhihui Liang,

Moldir Shyngys

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Abstract Nucleic acid nanostructures, derived from the assembly of nucleic building blocks (e.g., plasmids and oligonucleotides), are important intracellular carriers therapeutic cargoes widely utilized in preclinical nanomedicine applications, yet their clinical translation remains scarce. In era “translational nanotechnology”, a deeper mechanistic understanding interactions nanostructures with cells vivo will guide development more efficacious nanomedicines. This review showcases recent progress dissecting four key types (i.e., tile‐based, origami, spherical acid, nanogel) rodents over past five years. Emphasis lies on cellular‐level distribution various organs tissues cellular responses induced by entry. Next, spirit translation, this features latest large animals humans. Finally, offers directions for studying both materials biology perspectives concludes some regulatory updates.

Language: Английский

Citations

3
Functionalities of pH-responsive DNA nanostructures in tumor-targeted strategies DOI
Lingrong Wen, Min Wang

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Nanostructures integrating pH-sensitive DNA motifs have emerged as versatile platforms for active tumor targeting, owing to their ability undergo conformation changes in response the common acidic environment of extracellular matrix and endocytosis pathway. This review summarizes latest advances design application various pH-responsive nanostructures tumor-targeted strategies, including recognition, cell imaging, dynamic nanocarrier construction, controlled drug release. A comprehensive framework pH-controlled multi-stage targeting is introduced, addressing divergences strategies intracellular environments. The unique attributes, practical performance challenges are also critically discussed provide guidance future development this field.

Language: Английский

Citations

3
High‐Throughput, Label‐Free Detection of DNA Origami in Single‐Cell Suspensions Using origamiFISH‐Flow DOI

Shana Alexander,

Wendy Xueyi Wang, Chung‐Yi Tseng

et al.

Small, Journal Year: 2024, Volume and Issue: 20(35)

Published: April 30, 2024

Structural DNA nanotechnology enables custom fabrication of nanoscale devices and promises diverse biological applications. However, the effects design on nanostructure (DN)-cell interactions in vitro vivo are not yet well-characterized. origamiFISH is a recently developed technique for imaging DNs cells tissues. Compared to use fluorescent tags, offers label-free structure-agnostic detection with significantly improved sensitivity. Here, extended quantify single-cell suspensions, including nonadherent such as subsets immune cells, via readout by flow cytometry. This method, referred origamiFISH-Flow, high-throughput (e.g., 10 000 per second) compatible immunostaining concurrent cell-type cell-state characterization. It shown that origamiFISH-Flow provides 20-fold higher signal-to-noise ratio DN compared dye labeling approaches, leading capture >25-fold more

Language: Английский

Citations

2
Analysis of Nanoparticles’ Effects on Drug-Induced Psoriasis DOI
Ankit Shah, Chelsea Sanders, Simone Difilippantonio

et al.

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 129 - 135

Published: Jan. 1, 2024

Language: Английский

Citations

0
Emerging Voices in Drug Delivery – Harnessing and Modulating Complex Biological Systems (Issue 2) DOI
Shawn C. Owen, Juliane Nguyen

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115293 - 115293

Published: March 21, 2024

Language: Английский

Citations

0
High-throughput, label-free detection of DNA origami in single-cell suspensions using origamiFISH-Flow DOI Creative Commons

Shana Alexander,

Wendy Xueyi Wang, Chung‐Yi Tseng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 2, 2023

Abstract Structural DNA nanotechnology enables custom fabrication of nanoscale devices and promises diverse biological applications. However, the effects design on nanostructure (DN)-cell interactions in vitro vivo are not yet well-characterized. origamiFISH is a recently developed technique for imaging DNs cells tissues. Compared to use fluorescent tags, offers label-free structure-agnostic detection with significantly improved sensitivity. Here, we extend quantifying single-cell suspensions, including nonadherent such as subsets immune cells, via readout by flow cytometry. This method, referred origamiFISH-Flow, high-throughput (e.g., 10,000 per second) compatible immunostaining concurrent cell-type -state characterization. We demonstrate that origamiFISH-Flow enhances signal-to-noise ratio up 20-fold compared dye labeling approaches, leading capture >25-fold more DN + at low, single-picomolar uptake concentrations. additionally show profile shape-specific patterns across cell lines splenocytes quantify accumulation lymphoid organs. Together, new tool interrogate tissues, while providing insights tailoring their designs bio-applications.

Language: Английский

Citations

0