Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 92, P. 105325 - 105325
Published: Dec. 30, 2023
Language: Английский
Biomolecules, Journal Year: 2023, Volume and Issue: 14(1), P. 4 - 4
Published: Dec. 20, 2023
Osteonecrosis of the femoral head (ONFH) is a common refractory orthopedic disease, which one causes hip pain and dysfunction. ONFH has very high disability rate, associated with heavy burden to patients, families, society. The pathogenesis not completely clear. At present, it believed that mainly includes coagulation dysfunction, abnormal lipid metabolism, an imbalance osteogenic/adipogenic differentiation, poor vascularization repair. prevention treatment always been great challenge for clinical surgeons. However, recent studies have emphasized use mesenchymal stem cells (MSCs) treat steroid-induced (SONFH) promising therapy. This review focuses on role molecular mechanism epigenetic regulation in progress MSCs SONFH, discusses significance latest research SONFH from perspective epigenetics.
Language: Английский
Citations
6
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 30, 2024
Antisense oligonucleotides (ASOs) belong to promising therapeutic molecules for the treatment of neurologic, muscular and metabolic disorders. Several ASOs have been approved so far more than a hundred clinical trials are currently underway covering dozen areas including cancer. Yet, mechanisms internalization, cell trafficking action these remain poorly understood. Moreover, with only small fraction reaching correct cellular compartment following systemic delivery, majority targeted diseases requires recurrent injections ASOs. A deeper understanding would guide improvement their potency thus, reduce amount delivered potential side-effects. Here, using CRISPR screen, we investigated intracellular proteins involved in efficiency whole genome approach identified several regulators which could significantly impact melanoma cells. From proteins, validated WD Repeat Domain 91 (WDR91), regulator endosomal maturation, as modulator whose depletion inhibits ASO productive activity. This study provides first list modulators biologically relevant functional assay estimate role proteins. In conclusion, data lead better favoring uptake or improved escape
Language: Английский
Citations
1
Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 2(3), P. 132 - 155
Published: Dec. 23, 2024
Proteins are an important therapeutic modality in modern medicine. However, their inherent inability to traverse cell membranes essentially limits application extracellular targets. Recent advances intracellular protein delivery have enabled access traditionally "undruggable" targets and paved the way precisely modulate cellular functions. This Review provides a comprehensive examination of key mechanisms emerging technologies that facilitate transport functional proteins across membranes. Delivery methods categorized into physical, chemical, biological approaches, each with distinct advantages limitations. Physical enable direct entry but often pose challenges related invasiveness technical complexity. Chemical strategies offer customizable solutions enhanced control over targeting uptake, yet may face issues cytotoxicity scalability. Biological approaches leverage naturally occurring processes achieve efficient transport, though regulatory production consistency remain hurdles. By highlighting recent advancements, challenges, opportunities within approach, this review underscores potential unlock new pathways transform drug development paradigms.
Language: Английский
Citations
1
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 503, P. 158522 - 158522
Published: Dec. 10, 2024
Language: Английский
Citations
1
Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 92, P. 105325 - 105325
Published: Dec. 30, 2023
Language: Английский
Citations
3