EAACIMolecular Allergology User's Guide 2.0

Pediatric Allergy and Immunology, Journal Year: 2023, Volume and Issue: 34(S28)

Published: March 1, 2023

Abstract Since the discovery of immunoglobulin E (IgE) as a mediator allergic diseases in 1967, our knowledge about immunological mechanisms IgE‐mediated allergies has remarkably increased. In addition to understanding immune response and clinical symptoms, allergy diagnosis management depend strongly on precise identification elicitors reaction. past four decades, innovations bioscience technology have facilitated production well‐defined, highly pure molecules for component‐resolved (CRD), allowing personalized disease individual patients. The first edition “EAACI Molecular Allergology User's Guide” (MAUG) 2016 rapidly became key reference clinicians, scientists, interested readers with background allergology, immunology, biology, medicine. Nevertheless, field molecular allergology is moving fast, after 6 years, new EAACI Taskforce was established provide an updated document. Guide 2.0 summarizes state‐of‐the‐art information allergen molecules, their relevance, application diagnostic algorithms practice. It designed both, clinicians guiding health care professionals through overwhelming list different available testing. Further, it provides relevance allergenic gives overview basic test formats, tests measure exposure.

Language: Английский

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Comparison of the new RIDA qLine Allergy multiparameter immunoblot and the ImmunoCAP Specific IgE test for the identification of clinically relevant food and aeroallergen allergies

Frontiers in Allergy, Journal Year: 2025, Volume and Issue: 5

Published: Jan. 10, 2025

Multiparameter immunoblot testing is increasingly used as an alternative to multiple individual IgE analyses for type 1 allergies. This study investigated the performance of inexpensive method, RIDA qLine allergy test system (R-Biopharm AG), vs. current gold standard. Three study-specific panels with 57 allergens (food and aeroallergens) were analyzed in serum samples from 200 patients signs symptoms IgE-mediated allergies, using both Allergy reference ImmunoCAP Specific (Thermo Fisher Scientific). In case divergent results, corresponding remeasured secondary 3gAllergy Universal Kit (Siemens). The clinical diagnoses included. addition, a cross-reactive carbohydrate determinant (CCD)-inhibitor was decrease incidence positive CCD bands. mean overall agreement all food aeroallergens methods 94.9%. Qualitative evaluation showed average negative percent 98.9% 75.1% after methods. additional treatment inhibitor successfully reduced occurrence reactivity retesting. comparative analysis specific detection revealed strong correlation between levels measured across these platforms presentations, while also highlighting necessity careful contextual interpretation results. Standardized allergen extracts would improve independent comparisons different

Language: Английский

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Isotype diversity of antibodies specific for component allergens in the context of allergic diseases: eosinophilic esophagitis (EoE), asthma, and the alpha-gal syndrome (AGS)

Frontiers in Allergy, Journal Year: 2025, Volume and Issue: 5

Published: Feb. 4, 2025

From the earliest days of studying reagins in allergic sera that give rise to Prausnitz-Kuestner reaction, there was evidence were other types antibodies (Ab) specific for allergens, particularly those induced by immunotherapy. By 1980, not only IgE recognized and could be measured, but presence isotypes including IgG IgA patients with well established. time onwards development monoclonal made it possible distinguish measure such as IgG4, IgG2, IgG3. Over past 40 years two things have dominated field- firstly, techniques measuring isotype allergens improved steadily. Secondly, several different diseases or phenomena been identified which diversity has become a major issue. Prior 1990 occasional cases eosinophilic esophagitis (EoE) had identified, since then they common. Most positive skin tests and/or Ab cow's milk wheat, became obvious most EoE are primarily related IgE. Today is clear IgG4 these play significant role EoE. In 2000 first reports children developing tolerance cat allergen appeared. this depends on high levels increasing response against Fel d 1 . The recent novel disease alpha-gal syndrome (AGS). This condition based oligosaccharide galactose alpha,1-3-galactose, tick bites. However, case already known all immunocompetent primates IgM oligosaccharide. Furthermore, whether isotypes, IgG1 IgG3, inflammatory Overall, current future investigation requires careful assessment diverse addition

Language: Английский

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Gastropod Allergy: A Comprehensive Narrative Review

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(6), P. 5950 - 5964

Published: June 13, 2024

Food allergies have increased significantly in recent decades, with shellfish being a leading cause of food allergy and anaphylaxis worldwide, affecting both children adults. The prevalence is estimated to be approximately 0.5–2.5% the general population, varying by geographical location, age, consumption habits. Although mollusk has risen, remains unknown. While extensive research focused on crustacean allergies, particularly those related gastropods, received comparatively less attention. Clinical manifestations range from localized symptoms life-threatening systemic reactions, such as anaphylaxis. Notably, severe bronchospasm predominant clinical feature cases involving gastropods. Several allergens been identified mollusks, including paramyosin, tropomyosin, sarcoplasmic calcium-binding protein. In documented include heavy chain myosin, Der p 4 amylase. Diagnosis typically involves thorough history, skin testing, vitro quantification immunoglobulin (Ig) E, confirmation through an oral challenge, although latter reserved for selected cases. This narrative review highlights limited gastropod allergy. It provides comprehensive list purified recombinant discusses applications component-resolved diagnosis well current therapeutic developments.

Language: Английский

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Molecular Allergy Diagnosis in Clinical Practice: Frequently Asked Questions

Journal of Investigational Allergology and Clinical Immunology, Journal Year: 2021, Volume and Issue: 32(1), P. 1 - 12

Published: Nov. 24, 2021

In the last decades there has been a great progress in field of molecular biology allowing study sensitization to individual allergenic components an source, practice that termed Molecular Allergy Diagnosis (MD) or Component Resolved (CRD). The purpose present review is offer clinician practical approach use MD by answering frequently asked questions among physicians on how can help us improve allergy diagnosis our daily clinical practice. article divided three sections. First, brief importance for know main allergens different sources, their structure and vitro cross-reactivity before approaching (section A). Secondly core usefulness B) FAQS subject, finally section (C) interpretation integration with rest available tools diagnosis.

Language: Английский

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Appearance of food-dependent exercise-induced anaphylaxis as an inflammatory disease: a pediatric case report and differential diagnosis

Allergologia et Immunopathologia, Journal Year: 2023, Volume and Issue: 51(2), P. 52 - 58

Published: March 1, 2023

Anaphylaxis is the most serious of all allergic reactions. Despite advances in knowledge anaphylaxis, its clinical manifestations continue to be under-recognized. Indeed, proper diagnosis anaphylaxis often missed, and treatment delayed. The underlying causes are still under investigation globally. Inflammation represents cornerstone pathophysiology anaphylaxis. Food-dependent exercise-induced (FDEIA) a rare manifestation characterized by chronological sequence which food ingestion followed physical exercise leads Its mechanisms yet fully explained. We report case 14-year-old Chinese male who lost consciousness while undergoing activity at school. Several differential diagnoses were considered such as hypovolemic shock, septic anaphylactic shock or neurological adverse event. Finally, FDEIA was made. This highlights difficulties diagnosing management, especially when history not complete detailed.

Language: Английский

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An Overview of the Relevance of IgG4 Antibodies in Allergic Disease with a Focus on Food Allergens

Children, Journal Year: 2021, Volume and Issue: 8(5), P. 418 - 418

Published: May 20, 2021

Antibodies of the IgG4 isotype are strongly associated with allergic disease but have several properties such as not precipitating allergens, activating complement and poor binding to Fcγ receptors that argue against a pro-inflammatory role. In keeping that, antibodies striking feature response immunotherapy. two naturally occurring situations common low or absent IgE antibodies. The first example is children raised in house cat second eosinophilic esophagitis (EoE). many population-based cohorts, ownership early childhood decreased prevalence allergy at age 10. (i.e., EoE) novel form food mediated by related consuming cow’s milk wheat. EoE, patients proteins high > 10 µg/mL very 100 titers. Enigmatically these found deposits wall their inflamed esophagus. factors given rise EoE remain unclear; however, changes processing over past 50 years, particularly ultra-heat treatment pressure homogenization milk, represent logical hypothesis.

Language: Английский

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Gastropod Allergy: A Comprehensive Narrative Review

Published: May 27, 2024

Food allergies have increased significantly in recent decades, with shellfish being a leading cause of food allergy and anaphylaxis worldwide, affecting both children adults. The prevalence is estimated to be approximately 0.5-2.5% the general population, varying by geographical location, age, consumption habits. Although mollusk has risen, remains unknown. While extensive research focused on crustacean allergies, particularly those related gastropods, received comparatively less attention. Clinical manifestations range from localized symptoms life-threatening systemic reactions, such as anaphylaxis. Notably, severe bronchospasm predominant clinical feature cases involving gastropods. Several allergens been identified mollusks, including paramyosin, tropomyosin, sarcoplasmic calcium-binding protein. In documented include heavy chain myosin, Der p 4 amylase. Diagnosis typically involves thorough history, skin testing, vitro quantification immunoglobulin (Ig) E, confirmation through oral challenge, although latter reserved for selected cases. This review highlights limited gastropod allergy, date, necessity further investigations better understand implications within this class.

Language: Английский

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A multi-AS-PCR-coupled CRISPR/Cas12a assay for the detection of ten single-base mutations

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1320, P. 343027 - 343027

Published: July 26, 2024

Language: Английский

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Precision medicine in the allergy clinic: the application of component resolved diagnosis

Expert Review of Clinical Immunology, Journal Year: 2022, Volume and Issue: 18(2), P. 145 - 162

Published: Jan. 26, 2022

A precise diagnosis is key for the optimal management of allergic diseases and asthma. In vivo or in vitro diagnostic methods that use allergen extracts often fail to identify molecules eliciting reactions.Component-resolved (CRD) has solved most limitations extract-based procedures currently valuable tool precision allergy clinic, venom food allergy, asthma, rhinitis, atopic dermatitis. Its implementation daily practice facilitates: a) distinction between genuine multiple sensitizations cross-reactive sensitization polysensitized patients; b) prediction a severe, systemic reaction insect allergy; c) selection immunotherapy based on patient profile. This paper describes its main advantages disadvantages, cost-effectiveness future perspectives.The strategy CRD part new concept immunology, which aims improve diseases.

Language: Английский

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