Cryobiology, Journal Year: 2020, Volume and Issue: 95, P. 60 - 67
Published: June 10, 2020
Language: Английский
Reproduction in Domestic Animals, Journal Year: 2024, Volume and Issue: 59(1)
Published: Jan. 1, 2024
Abstract The use of antioxidants for semen preservation prevents oxidative damage caused by reactive oxygen species (ROS). One the most promising natural is resveratrol, a phytoalexin derived from plants, grapes, berries, peanuts and red wine. To evaluate effect resveratrol on quality redox status cryopreserved bovine semen. Five bulls were subjected to electroejaculation obtain 15 ejaculates. Each ejaculate was extended with tris‐egg yolk‐glycerol‐based medium divided into six aliquots supplemented 0 (control), 10, 20, 30, 40 50 μM resveratrol. Semen frozen liquid nitrogen vapours. Post‐thawing, motility kinetics evaluated using computer‐assisted sperm analysis (CASA) system, membrane integrity hypoosmotic test (HOST), morphology staining eosin–nigrosin, vitality fluorescence microscopy SYBR14/IP probes. Total antioxidant capacity (TAC) ABTS •+ assay ROS spectrofluorimetry H 2 DCFDA probe. For statistical linear models adjusted means compared Tukey test. All concentrations reduced post‐thawed sperm. Supplementation kinetics, between 30 alterations in observed. However, at increased TAC 20 μM, it production Resveratrol appears have dose‐dependent which higher doses produce greater alterations, however, can increase during freezing. It concluded that reduce its 10 reduces post‐thawing quality.
Language: Английский
Citations
8
Antioxidants, Journal Year: 2020, Volume and Issue: 9(10), P. 1023 - 1023
Published: Oct. 21, 2020
Reproduction is a complex process that substantially affected by environmental cues, specifically feed/diet and its components. Farm animals as herbivorous are exposed to large amount of polyphenols present in their natural feeding system, alternative feed resources (shrubs, trees, agro-industrial byproducts), polyphenol-enriched additives. Such exposure has increased because the well-known antioxidant properties polyphenols. However, date, argumentation around impacts on reproductive events debatable. Accordingly, intensive inclusion diets breeding media for assisted techniques needs further investigation, avoiding any source waste achieving maximum benefits. This review illustrates recent findings connecting dietary consumption from different sources (conventional unconventional feeds) with performance farm animals, underpinned vitro studies this field. update will help formulating proper diets, optimizing introduction new plant species, additives improving function, possible wastes maximizing
Language: Английский
Citations
50
Frontiers in Chemistry, Journal Year: 2021, Volume and Issue: 9
Published: Feb. 26, 2021
Cryopreservation prolongs the storage time of cells and plays an important role in modern biology, agriculture, plant science medicine. During cryopreservation, may suffer many damages, such as osmotic dehydration, large ice puncture oxidative damages from reactive oxygen species (ROS). Classic cryoprotectants (CPAs) are failing to dispose ROS, while antioxidants can turn ROS into harmless materials regulate stress. The combination CPAs improve efficiency cryopreservation negative results occur by misuse antioxidants. This paper discussed feasibility cryopreservation.
Language: Английский
Citations
50
Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 266, P. 107495 - 107495
Published: May 16, 2024
Language: Английский
Citations
7
IRAQI JOURNAL OF AGRICULTURAL SCIENCES, Journal Year: 2023, Volume and Issue: 54(4), P. 1074 - 1083
Published: Aug. 29, 2023
This study was conducted to explain the effect of adding different resveratrol (Res) concentrations soybean-lecithin extender on some post-cryopreserved (PC) semen characteristics Iraqi buffalo bulls for preservation periods. Eight mature were used, and polled equally divided into four groups within one trial. Three Res (100, 150, 200 μM) added extender, comparisons in response made with control group (soybean-lecithin Res-0). The differences among sperm cell individual motility percentages at periods lacked significance. Greater (P≤0.01) acrosome integrity percentage noticed Res-150 compared C Res-200 cooling, 48 hrs., 2 months PC. Lesser malondialdehyde DNA damage observed as other groups, Higher total antioxidant activity shown Res-100 In conclusion, enhanced PC
Language: Английский
Citations
15
Theriogenology, Journal Year: 2020, Volume and Issue: 152, P. 106 - 113
Published: April 29, 2020
Language: Английский
Citations
39
Journal of King Saud University - Science, Journal Year: 2020, Volume and Issue: 33(1), P. 101226 - 101226
Published: Nov. 4, 2020
Despite the widespread use of frozen or refrigerated mammalian spermatozoa, their quality remains low as they are highly sensitivity to injuries during preservation and thawing. Moreover, in vitro conditions spermatozoa storage may affect sperm thereby oocyte fertilization, cleavage, blastocyst development. Recently, antioxidants have been employed semen extenders for livestock different protocols compensate depletion endogenous antioxidant concentration seminal plasma due dilution well counteract oxidative stress minimize free radical generation. The present article reviews most effective enzymatic non-enzymatic used preservation.
Language: Английский
Citations
34
Cryobiology, Journal Year: 2021, Volume and Issue: 101, P. 125 - 134
Published: April 30, 2021
Language: Английский
Citations
32
Antioxidants, Journal Year: 2022, Volume and Issue: 11(9), P. 1808 - 1808
Published: Sept. 14, 2022
While critical for male fertility preservation, cryopreservation damage reduces sperm quality and fertilization potential. This study investigated whether the addition of mitochondrial-targeted, antioxidant compounds, also known as Mitochondrial activators, to medium could protect during cryopreservation. For this, semen samples from men undergoing IVF/ICSI treatment, which were donated research, underwent in absence or presence BGP-15, MitoQ L-carnitine. Fresh thawed same participant analyzed indicators quality: viability, kinetics, mitochondrial reactive oxygen species (ROS) levels, Membrane Potential (MMP) DNA damage. Cryopreservation significantly reduced viability motility predicted mucous penetration. L-carnitine improved motility, whilst L-Carnitine prevented loss Both BGP-15 oxidative damage, but only fragmentation. More importantly, increased predictive penetration MMP oxidation. Our results show that improves post-thawing, highlighting potential antioxidants improve long-term preservation males.
Language: Английский
Citations
20
Animals, Journal Year: 2023, Volume and Issue: 13(14), P. 2300 - 2300
Published: July 13, 2023
The present review aims to provide an overview of the assay methods for quantification ROS and principal enzymatic antioxidants as biomarkers oxidative stress in erythrocytes spermatozoa small domestic ruminants. A complete literature search was carried out PubMed, Scopus World Wide Web using relevant keywords focusing on last five years (2018-2023). Among spectrophotometry, fluorometry chemiluminescence, most widely used method is fluorometry, probably because it allows simultaneously several ROS, different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent reports only spectrophotometric methods, many which use commercial kits. a less sensitive but cheapest suitable both samples are highly concentrated ruminant species. All considered this have been found be appropriate; general, differences related their costs sensitivity. Quantification activity may find application study welfare health status ruminants monitoring livestock production.
Language: Английский
Citations
11