Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 11, 2024

At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing maintenance of cell lifespan. In this context, study proteins that could protect during high relevance. The 70 kDa Heat Shock Protein (HSP70) an anti-apoptotic protein has been reported to be relevant survival. Thus, we explored role HSP70 prolonged at 17 °C. Six pools were incubated with YM-1 (0, 0.05, 0.1 and 0.2 μM), inhibitor, stored °C 21 days. On days 0, 4, 10, 14 21, quality function evaluated through flow cytometry Computer-Assisted Sperm Analysis (CASA), activity chromatin condensation also determined. While inhibition increased progressive motility, Ca2+ Reactive Oxygen Species (ROS) levels, mitochondrial first 10 storage, it had a detrimental effect on motility after spite this, viability was not altered. We can conclude contributes because keeps low, which needed function.

Language: Английский

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Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 305 - 305

Published: Jan. 22, 2025

Spermatozoa are highly specialized male cells that characterized by a unique ability to move, which is critical factor for successful fertilization. The relative simplicity of motility assessment, especially in livestock, has made it widely used parameter evaluating ejaculate quality or cryopreserved semen the clinical field, and an advanced tool reproductive physiology toxicology research. Technological advances image analysis computational methods have substantially increased its accuracy through use computer-assisted sperm (CASA) minimize subjective bias assessments. Nevertheless, this more objective method still presents some significant challenges, including variability sample preparation, imaging conditions, analytical parameters. These issues contribute inconsistency impair reproducibility comparability data between laboratories. implementation standardized protocols, combined with comprehensive training rigorous evaluation, can serve mitigate emerging inconsistencies. In addition, vitro conditions under CASA analyses performed often differ significantly from natural environment female tract vivo. This review discusses methodologies, issues, limitations using CASA, particular focus on boar as important agricultural biomedical model species system used.

Language: Английский

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Techniques to Determine Mammalian Sperm Capacitation

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 463 - 495

Published: Jan. 1, 2025

Language: Английский

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Bioinformatics on Sperm Subpopulations Using Computer-Assisted Sperm Analysis (CASA)

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 235 - 247

Published: Jan. 1, 2025

Language: Английский

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Modeling diffusive search by non-adaptive sperm: Empirical and computational insights

PLoS Computational Biology, Journal Year: 2025, Volume and Issue: 21(4), P. e1012865 - e1012865

Published: April 17, 2025

During fertilization, mammalian sperm undergo a winnowing selection process that reduces the candidate pool of potential fertilizers from ~10 6 -10 11 cells to 10 1 2 (depending on species). Classical competition theory addresses positive or ‘stabilizing’ acting phenotypes within populations organisms but does not strictly address developmental consequences traits among individual are under purifying during fertilization. It is latter utmost concern for improving assisted reproductive technologies (ART) because low-fitness may be inadvertently used fertilization interventions rely heavily artificial selection, such as intracytoplasmic injection (ICSI). Importantly, some form in nearly all forms ART (e.g., differential centrifugation, swim-up, hyaluronan binding assays, etc.). To date, there no unifying quantitative framework (i.e., selection) synthesizes causal mechanisms with observed natural variation traits. In this report, we reframe physiological function collective diffusive search and develop multi-scale computational models explore dynamics constrain fitness Several experimentally useful concepts developed, including probabilistic measure well an information theoretic magnitude each which assessed systematic increases microenvironmental selective pressure motility patterns.

Language: Английский

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Therapeutic potential of Sertoli cells in vivo: alleviation of acute inflammation and improvement of sperm quality

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 4, 2024

Language: Английский

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Cryptic female choice within individual males – A neglected component of the postmating sexual selection?

Journal of Evolutionary Biology, Journal Year: 2022, Volume and Issue: 35(11), P. 1407 - 1413

Published: Aug. 21, 2022

Cryptic female choice (CFC) is commonly assumed to act only in polyandrous mating systems, which allows females bias fertilization towards the sperm of particular males. However, accumulated evidence has demonstrated that show significant phenotypic and genotypic variation also within single ejaculates, have important consequences for offspring phenotype fitness. Here, I argue these neglected sources intra-male often allow CFC individual males facilitate genetically compatible (or otherwise preferred) haplotypes. In this article, explain prerequisites within-male CFC, criteria demonstrating it summarize emerging selection process. Then, evaluate prevalence review its potential evolutionary consequences. The aim article broaden current definition by all both internally externally fertilizing species. Incorporation concept into models sexual may provide novel insights deeper understanding selective factors driving evolution systems reproductive proteins. Finally, haplotypes increase our non-Mendelian inheritance.

Language: Английский

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The impact of Origanum Vulgare essential oil supplementation on sperm motility and subpopulation alterations in bulls, dogs, and rabbits

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 170, P. 105200 - 105200

Published: Feb. 26, 2024

Language: Английский

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The future of equine semen analysis

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(6)

Published: March 11, 2024

We are currently experiencing a period of rapid advancement in various areas science and technology. The integration high throughput 'omics' techniques with advanced biostatistics, the help artificial intelligence, is significantly impacting our understanding sperm biology. These advances will have an appreciable impact on practice reproductive medicine horses. This article provides brief overview recent field spermatology how they changing assessment quality. written from authors' perspective, using stallion as model. aim to portray changes occurring motility kinematics, flow cytometry, implementation technologies, use intelligence/self-learning data analysis. also briefly discuss some can be readily available practitioner, through 'on-farm' devices telemedicine.

Language: Английский

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Morphometric Differences in Sperm Subpopulations in Frozen-Thawed Semen of Two Bovine Subspecies

Published: Aug. 22, 2024

[Objetive] The objective of the present study was to characterize different sperm subpopulations based on morphometric parameters frozen-thawed semen in two bovine subspecies using a CASA system. [Methodology] experiment carried out at Costa Rica Institute Technology from May December 2023. Spermatozoa 10 bulls (five animals each species Bos taurus and indicus) were evaluated after thawing doses by an ISAS®v1, computer-assisted analysis (CASA)-Morph Sub-populations head spermatozoa characterized multivariate procedures such as principal components (PCs) clustering methods (k-means model) [Results] ejaculate male exhibits significant heterogeneity comprises diverse with differing patterns. Three identified three PCs: t size, shape, symmetry degree which it pyriform. proportions varied two-bovine subspecies; indicus. Results indicated that SP1, SP2, SP3 for PC criteria these differences relevant. variability morphometry assessments underscores need standardize evaluation protocols [Conclusions] These findings highlight importance knowing diversity between both improve vitro tests distinguish subspecies.

Language: Английский

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