Nanoscale Advances,
Journal Year:
2023,
Volume and Issue:
5(14), P. 3705 - 3716
Published: Jan. 1, 2023
Bionanoparticles
comprised
of
naturally
occurring
monomers
are
gaining
interest
in
the
development
novel
drug
transportation
systems.
Here
we
report
on
stabilisation,
cellular
uptake,
and
macrophage
clearance
nanotubes
formed
from
self-assembling
gp053
tail
sheath
protein
vB_EcoM_FV3
bacteriophage.
To
evaluate
potential
bacteriophage
protein-based
as
therapeutic
nanocarriers,
investigated
their
internalisation
into
colorectal
cancer
cell
lines
professional
macrophages
that
may
hinder
applications
by
clearing
nanotube
carriers.
We
fused
with
a
SNAP-tag
self-labelling
enzyme
demonstrated
its
activity
is
retained
assembled
nanotubes,
indicating
such
carriers
can
be
applied
to
deliver
biomolecules.
Under
physiological
conditions,
stabilisation
PEGylation
was
required
prevent
aggregation
yield
stable
solution
uniform
nano-sized
structures.
Colorectal
carcinoma
cells
primary
metastatic
tumours
internalized
SNAP-tag-carrying
different
efficiencies.
The
entered
HCT116
via
dynamin-dependent
SW480
-
dynamin-
clathrin-dependent
pathways
were
accumulated
lysosomes.
Meanwhile,
peritoneal
phagocytosed
highly
efficient
manner
through
actin-dependent
mechanisms.
Macrophage
enhanced
inflammatory
activation
but
dampened
isolated
aged
animals.
Altogether,
our
results
demonstrate
cargo's
enzymatic
post-assembly
had
capacity
enter
cells.
Furthermore,
emphasise
importance
evaluating
nanocarrier
immune
under
conditions
mimicking
cancerous
environment.
Translational Oncology,
Journal Year:
2023,
Volume and Issue:
40, P. 101846 - 101846
Published: Dec. 1, 2023
The
treatment
of
cancer
patients
has
been
mainly
followed
using
chemotherapy
and
it
is
a
gold
standard
in
improving
prognosis
survival
rate
patients.
Oxaliplatin
(OXA)
third-platinum
anti-cancer
agent
that
reduces
DNA
synthesis
cells
to
interfere
with
their
growth
cell
cycle
progression.
In
spite
promising
results
OXA
chemotherapy,
the
process
drug
resistance
made
some
challenges.
commonly
applied
colorectal
(CRC)
as
malignancy
gastrointestinal
tract
when
CRC
increase
proliferation
metastasis,
they
can
obtain
chemotherapy.
A
number
molecular
factors
such
CHK2,
SIRT1,
c-Myc,
LATS2
FOXC1
have
considered
regulators
response
cells.
non-coding
RNAs
are
able
function
master
regulator
other
pathways
modulating
resistance.
There
close
association
between
mechanisms
apoptosis,
autophagy,
glycolysis
EMT
resistance,
so
apoptosis
inhibition,
pro-survival
autophagy
induction
stimulation
induce
anti-tumor
compounds
including
astragaloside
IV,
resveratrol
nobiletin
enhance
sensitivity
Nanoparticles
for
increasing
potential
suppression
reversing
employed
These
subjects
covered
this
review
article
shed
light
on
resulting
Bioengineering,
Journal Year:
2022,
Volume and Issue:
9(7), P. 294 - 294
Published: June 30, 2022
Currently,
breast-cancer
treatment
has
a
number
of
adverse
side
effects
and
is
associated
with
poor
rates
progression-free
survival.
Therefore,
radiolabeled
anti-EpCAM
targeted
biomimetic
coated
nanocarrier
(EINP)
was
developed
in
this
study
to
overcome
some
the
challenges.
The
double
emulsion
method
synthesized
poly(lactic-co-glycolic
acid)
(PLGA)
nanoparticle
Na131I
entrapped
core.
PLGA
human
red
blood
cell
membranes
labeled
epithelial
adhesion
molecule
(EpCAM)
antibody
enable
it
target
EpCAM
overexpression
by
cells.
Characterization
determined
EINP
size
as
295
nm,
zeta
potential
−35.9
mV,
polydispersity
0.297.
radiochemical
purity
>95%.
Results
efficacy
against
positive
MCF-7
breast
cancer
at
24,
48,
72
h
were
69.11%,
77.84%,
74.6%,
respectively,
demonstrating
that
EINPs
achieved
greater
cytotoxic
supported
NIS-mediated
uptake
than
non-targeted
131INPs
Na131I.
In
comparison,
fibroblast
(EpCAM
negative)
treated
had
significantly
lower
cytotoxicity
(p
<
0.05).
Flow
cytometry
fluorescence
imaging
visually
signified
delivery
specifically
cells
result
targeting.
Additionally,
favorable
safety
profile,
hemolysis.
Nanoscale Advances,
Journal Year:
2023,
Volume and Issue:
5(14), P. 3705 - 3716
Published: Jan. 1, 2023
Bionanoparticles
comprised
of
naturally
occurring
monomers
are
gaining
interest
in
the
development
novel
drug
transportation
systems.
Here
we
report
on
stabilisation,
cellular
uptake,
and
macrophage
clearance
nanotubes
formed
from
self-assembling
gp053
tail
sheath
protein
vB_EcoM_FV3
bacteriophage.
To
evaluate
potential
bacteriophage
protein-based
as
therapeutic
nanocarriers,
investigated
their
internalisation
into
colorectal
cancer
cell
lines
professional
macrophages
that
may
hinder
applications
by
clearing
nanotube
carriers.
We
fused
with
a
SNAP-tag
self-labelling
enzyme
demonstrated
its
activity
is
retained
assembled
nanotubes,
indicating
such
carriers
can
be
applied
to
deliver
biomolecules.
Under
physiological
conditions,
stabilisation
PEGylation
was
required
prevent
aggregation
yield
stable
solution
uniform
nano-sized
structures.
Colorectal
carcinoma
cells
primary
metastatic
tumours
internalized
SNAP-tag-carrying
different
efficiencies.
The
entered
HCT116
via
dynamin-dependent
SW480
-
dynamin-
clathrin-dependent
pathways
were
accumulated
lysosomes.
Meanwhile,
peritoneal
phagocytosed
highly
efficient
manner
through
actin-dependent
mechanisms.
Macrophage
enhanced
inflammatory
activation
but
dampened
isolated
aged
animals.
Altogether,
our
results
demonstrate
cargo's
enzymatic
post-assembly
had
capacity
enter
cells.
Furthermore,
emphasise
importance
evaluating
nanocarrier
immune
under
conditions
mimicking
cancerous
environment.
