Visualizing DNA/RNA, Proteins, and Small Molecule Metabolites within Live Cells DOI
Dongling Jia,

Minhui Cui,

Xianting Ding

et al.

Small, Journal Year: 2024, Volume and Issue: 20(46)

Published: Aug. 3, 2024

Live cell imaging is essential for obtaining spatial and temporal insights into dynamic molecular events within heterogeneous individual cells, in situ intracellular networks, vivo organisms. Molecular tracking live cells also a critical general requirement studying physiological processes biology, cancer, developmental neuroscience. Alongside this context, review provides comprehensive overview of recent research progress live-cell RNAs, DNAs, proteins, small-molecule metabolites, as well their applications diagnosis, immunodiagnosis, biochemical diagnosis. A series advanced techniques have been introduced summarized, including high-precision imaging, high-resolution low-abundance multidimensional multipath rapid computationally driven methods, all which offer valuable disease prevention, treatment. This article addresses the current challenges, potential solutions, future development prospects field.

Language: Английский

Cancer stem cells: advances in knowledge and implications for cancer therapy DOI Creative Commons

Xianjing Chu,

Wentao Tian,

Jiaoyang Ning

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 5, 2024

Abstract Cancer stem cells (CSCs), a small subset of in tumors that are characterized by self-renewal and continuous proliferation, lead to tumorigenesis, metastasis, maintain tumor heterogeneity. continues be significant global disease burden. In the past, surgery, radiotherapy, chemotherapy were main cancer treatments. The technology treatments develop advance, emergence targeted therapy, immunotherapy provides more options for patients certain extent. However, limitations efficacy treatment resistance still inevitable. Our review begins with brief introduction historical discoveries, original hypotheses, pathways regulate CSCs, such as WNT/β-Catenin, hedgehog, Notch, NF-κB, JAK/STAT, TGF-β, PI3K/AKT, PPAR pathway, their crosstalk. We focus on role CSCs various therapeutic outcomes resistance, including how affect content alteration related molecules, CSCs-mediated clinical value targeting refractory, progressed or advanced tumors. summary, efficacy, method is difficult determine. Clarifying regulatory mechanisms biomarkers currently mainstream idea.

Language: Английский

Citations

92
Visualizing DNA/RNA, Proteins, and Small Molecule Metabolites within Live Cells DOI
Dongling Jia,

Minhui Cui,

Xianting Ding

et al.

Small, Journal Year: 2024, Volume and Issue: 20(46)

Published: Aug. 3, 2024

Live cell imaging is essential for obtaining spatial and temporal insights into dynamic molecular events within heterogeneous individual cells, in situ intracellular networks, vivo organisms. Molecular tracking live cells also a critical general requirement studying physiological processes biology, cancer, developmental neuroscience. Alongside this context, review provides comprehensive overview of recent research progress live-cell RNAs, DNAs, proteins, small-molecule metabolites, as well their applications diagnosis, immunodiagnosis, biochemical diagnosis. A series advanced techniques have been introduced summarized, including high-precision imaging, high-resolution low-abundance multidimensional multipath rapid computationally driven methods, all which offer valuable disease prevention, treatment. This article addresses the current challenges, potential solutions, future development prospects field.

Language: Английский

Citations

2