From invasion to fish fodder: Inclusion of the brown algae Rugulopteryx okamurae in aquafeeds for European sea bass Dicentrarchus labrax (L., 1758)

Aquaculture, Journal Year: 2023, Volume and Issue: 568, P. 739318 - 739318

Published: Feb. 4, 2023

In keeping with the premises of Blue Circular Economy in European Union, present study explored possibility using invasive brown alga Rugulopteryx okamurae aquafeeds for sea bass (Dicentrarchus labrax). Assuming raw algae biomass could negatively impact animal performance, four experimental formulations were prepared, by including macroalgae material at 5%, crude (CR), enzymatically hydrolysed and fermented (EF), (E), or (F) R. biomass, which we tested against a control feed (CT). To evaluate effects diets, besides growth performance biometric parameters, devised toolbox focused on intestine intestinal function: i) ex-vivo epithelial resistance permeability Ussing chambers; ii) microbiota composition through NGS; iii) expression profiles selected markers integrity, transport, metabolism, immune response, qPCR. Our results show differentiated allometric among coupled epithelium alterations permeability, amino acid transport. Additionally, evidence dysbiosis contrasting responses between i.e. pro-inflammatory vs. anti-inflammatory, are also described. conclusion, believe that be suitable resource bass, although its use requires pre-treatment before inclusion. Otherwise, while fish still have positive gastrointestinal tract pays toll inflammatory processes.

Language: Английский

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¿Pueden las microalgas ser sustitutas del aceite y harina de pescado en dietas para acuicultura?

Encuentros en la Biología, Journal Year: 2025, Volume and Issue: 17(189)

Published: March 31, 2025

El pienso empleado en acuicultura incluye elevadas cantidades de aceite y harina pescado, ingredientes excelentes desde un punto vista nutricional, pero insostenibles medioambientalmente debido a que requieren la captura pequeños peces pelágicos para su elaboración. Por este motivo, comunidad científica busca alternativos permitan el desarrollo sostenible garanticen seguridad alimentaria población mundial, sin perjudicar rendimiento productivo bienestar los animales cultivados. En sentido, las microalgas se postulan como prometedores, sus buenas cualidades nutricionales sostenibilidad, ayudando así conservar medio ambiente.

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Microalgae as functional feed for Atlantic salmon: effects on growth, health, immunity, muscle fatty acid and pigment deposition

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Oct. 24, 2023

Microalgae are increasingly being investigated as functional feed additives in a variety of fish species, but our knowledge on how microalgae supplementation affects Atlantic salmon remains limited. We hypothesized that inclusion 8% the would improve performance, fatty acid and pigment deposition well health immunity reared recirculating aquaculture systems (RAS). fed smolts with five different enriched diets containing Tetraselmis chuii (TC), Arthrospira platensis (AP), Schizochytrium limacinum (SL) or Chlorella vulgaris , either intact (CVI) broken cell wall derivative (CVB) control diet (CD). After eight weeks feeding brackish water (13 psu), all groups were transferred to seawater (32 psu) for additional two weeks. Our results indicate CVB improved conversion protein retention, reduced condition factor (p < 0.05) compared diet. Voluntary intake decreased seawater, was similar among groups. The amount docosahexaenoic particularly high SL-fed alpha-linolenic CVI, TC 0.05). Following transfer, fat content monounsaturated acids muscle, while polyunsaturated increased. Lutein present muscle samples, highest concentrations found CVB-, CVI- TC-fed fish. In anterior intestine, induced differentially regulated trout 1 ( drtp1 ) expression CVB-fed fish, interleukin 10 receptor il1r2 & il10rb CVI-fed liver, CVI SL complement C1q like 2 c1ql2 expression, reducing serum amyloid A5 saa5 expression. Superoxide-dismutase concentration liver SL, myeloperoxidase most microalgae-fed conclusion, we show commercially relevant can be used promoting aspects without negatively affecting their growth performance when cultivated RAS.

Language: Английский

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Effects of feeding European seabass (Dicentrarchus labrax) juveniles with crude, hydrolysed and fermented biomass of the invasive macroalga Rugulopteryx okamurae (Ochrophyta)

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 34, P. 101877 - 101877

Published: Dec. 18, 2023

The increasing expansion of the invasive Asian alga Rugulopteryx okamurae along Andalusian coasts is a major challenge to marine biodiversity, and urgent coordinated measures are required for its removal or elimination. Among different actions, biotechnological valorisation could be strategy management alien biomass within frame Blue Economy. This work evaluates potential R. biomass, either raw processed by treatments based on enzymatic hydrolysis fermentation processes, used as dietary ingredient in juvenile seabass (Dicentrarchus labrax). These treatments, intended primarily improve nutrient availability, might also decrease content undesirable potentially harmful algal metabolites, such diterpenoid dilkamural. To this end, 90-day feeding trial was carried out using four experimental diets containing 5% (w/w) crude biomass. results obtained revealed that inclusion diet caused adverse effects fish growth, yielding lower values than those control group (p < 0.05), although detrimental effect negligible when algae prior feeds. In terms muscle composition, supplemented with induced an increase protein content, especially fed pre-treated Qualitative differences 0.05) were found fatty acid profile, significant ARA DHA contents algae-supplemented diets. A reduction 0.05)in liver lipid oxidation evidenced algae-enriched diets, treated Finally, negative gut functionality, these not observed previously processed. conclusion, functional sea bass, provided pre-treated, corroborated efficacy low-cost treatment method

Language: Английский

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Effects of Phytase and Microalgae Supplementation on the Utilization of Aquafeeds for European Seabass (Dicentrarchus labrax) Formulated with a High Inclusion Level of Plant Protein

Aquaculture Research, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 17

Published: May 6, 2024

Numerous studies have reported the disadvantages of using plant protein in aquafeeds owing to presence antinutritional factors. Particularly, phytate can be overcome by dietary supplementation with exogenous phytase. On other hand, use microalgae has been proven a valuable strategy for enhancing digestive functionality fish fed diets high content. It was hypothesized that simultaneous inclusion phytase useful improve growth performance and content protein. In this study, effects feeds supplemented different levels (FTU) 2.5% blend Arthrospira platensis Nannochloropsis gaditana were evaluated on performance, metabolism, gut European seabass (Dicentrarchus labrax) juveniles. Fish ad libitum 83 days five containing enzyme ratios (FTU), triplicate: (i) control (0 FTU); (ii) 500 FTU; (iii) 1,000 (iv) 2,000 (v) 10,000 FTU. At end feeding period, zootechnical indexes estimated, biological samples withdrawn physiological determinations. The results obtained showed an overall improvement productive efficiency, general intestinal highest (2,000 FTU). Final weight (75.51 ± 1.01 g vs. 66.76 1.26 g, p=0.005 ), SGR (1.15 0.02 1.00 0.02, p=0.006 ). feed efficiency (0.80 0.02% day−1 0.67 0.01% day−1, p=0.012 ) higher chemical composition muscle plasma metabolites did not vary among treatments, except glucose low glycogen liver FTU group. Focusing functionality, activities tended microalgae, significant differences found trypsin, chymotrypsin, alkaline phosphatase 1,000, 2,000, FTU, leucine aminopeptidase ( p<0.0001 Analysis morphology revealed combined increased villi height decreased diameter enterocyte height. at level seems lack influence parameters whereas only impacted growth, nutrient utilization, functionality. addition, faecal stripping final portion intestines, from which phosphorus nitrogen quantified. Apparent digestibility coefficient higher, 74.3% 77.8%, respectively, compared (52.4%). decrease amount P shed excreta animals phytase, fact evidenced absorption element. Overall, pointed out used 10,000, even combination as approach reducing costs boost fish, while minimizing environmental impacts juvenile farms.

Language: Английский

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Evaluation of the Combined Administration of Chlorella fusca and Vibrio proteolyticus in Diets for Chelon labrosus: Effects on Growth, Metabolism, and Digestive Functionality

Animals, Journal Year: 2023, Volume and Issue: 13(4), P. 589 - 589

Published: Feb. 7, 2023

This study aimed to evaluate the combined effect of dietary Chlorella fusca and ethanol-inactivated Vibrio proteolyticus DCF12.2 (C + V diet) in Chelon labrosus juveniles, highlighting their nutritional, physiological, morphological effects. The results showed that inclusion C. V. significantly enhanced growth performance feed utilization compared control group. C diet increased fish lipid quality index (FLQ), n-3 polyunsaturated fatty acids, n-3/n-6 ratio, which might be beneficial terms human nutrition. considerably carbohydrate metabolic activity by statistically boosting plasma glucose. conjunction with enzyme as well intestinal absorption capacity found In conclusion, experimental was suitable for feeding labrosus, increasing nutritional characteristics muscle intestine, without causing tissue damage.

Language: Английский

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Potential Effects of Microalgae-Supplemented Diets on the Growth, Blood Parameters, and the Activity of the Intestinal Microbiota in Sparus aurata and Mugil cephalus

Fishes, Journal Year: 2023, Volume and Issue: 8(8), P. 409 - 409

Published: Aug. 9, 2023

This work aimed to assess the suitability of a microalgal blend as dietary ingredient for feeding juveniles marine carnivorous and herbivorous teleost, is case Sparus aurata Mugil cephalus, respectively, isolate microorganisms from different media characterize them on base their enzymatic activities antagonism against important fish pathogens. Thirty each species (70 ± 3.2 g S. mean weight 47 2.8 M. cephalus weight) were distributed in four tanks (15 individuals each) corresponding independent treatments (control microalgae diets designed species). Fish fed ad libitum 108 days. At end trial, weighed, plasma, liver, perivisceral fat, entire intestines obtained evaluation growth performance metabolic assessment. Furthermore, 117 bacterial strains isolated culture gastrointestinal tract further characterized potential use probiotics aquaculture. microalgae-supplemented diet (25% inclusion) showed significant increase gain, specific rate, feed efficiency, hepatosomatic, intestine length indices. However, somatic indices not affected by experimental diets. Plasma samples revealed higher levels glucose triglycerides decrease cortisol levels. No differences found any biochemical parameters among cephalus. In conclusion, both demonstrated favorable adaptation nutritional formulation employed this study, UMA-169 UMA-216 (both identified Bacillus pumilus) could be considered aquaculture they might benefit host health improving digestion absorption energy sources minimizing colonization pathogenic species.

Language: Английский

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Microalgae and phytase dietary supplementation improved growth and gut microbiota in juvenile European seabass (Dicentrarchus labrax)

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Sept. 6, 2024

Language: Английский

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Feed Additives Based on N. gaditana and A. platensis Blend Improve Quality Parameters of Aquacultured Gilthead Seabream (Sparus aurata) Fresh Fillets

Fishes, Journal Year: 2024, Volume and Issue: 9(6), P. 205 - 205

Published: May 31, 2024

The aim of this research is to explore the potential effects two microalgae-based additives included in finishing feeds on quality and shelf-life seabream fillets. In a 41-day feeding trial, specimens were fed with experimental aquafeeds containing 10% bioactive supplements. These consisted blend Nannochloropsis gaditana Arthrospira platensis biomass, which was utilized as either raw (LB-CB) or enzymatically hydrolyzed (LB-CBplus). A control group received microalgae-free diet. results showed that functional improved nutritional profile fillets, increasing protein PUFA-n3 contents while reducing atherogenic index, especially for LB-CBplus treatment. also enhanced texture parameters (hardness chewiness) fillets during initial 5 days under cold storage. Regarding skin pigmentation, increased greenish yellowish coloration compared fish, mostly attributed inclusion crude algal biomass (LB-CB). Moreover, diets enriched microalgae effectively delayed muscle lipid oxidation processes refrigeration up 12 days, exhibiting higher antioxidant effects. findings highlight enhance both organoleptic

Language: Английский

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Body condition of stranded Razorbills and Atlantic Puffins in the Western Mediterranean

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(9)

Published: Sept. 1, 2024

Abstract Annual mass migrations of seabirds between their breeding and wintering grounds are critical for ensuring survival reproductive success. It is essential to comprehend physical condition in order identify the causes death facilitate conservation efforts. This study focuses on evaluating age, body index, metabolites liver muscle (triglycerides, glycerol, glycogen, cholesterol, lactate, glucose) stranded Razorbills ( n = 84) Atlantic puffins 11). The was conducted along Andalusian coast Spain during winter season 2022–2023. examined individuals metabolic state determine potential factors that may have caused deaths. found majority were juveniles. Both species exhibited low levels carbohydrate (glucose glycogen) tissues high lactate muscles. These findings could suggest had undergone prolonged, strenuous exercise, demanding energy anaerobic pathways, which been associated with migration. highlights significance adhering standardized protocols when assessing seabirds. Doing so can help A proposed index condition, incorporates biometric measurements individual provides a comprehensive means understanding health these unique species. underscores importance further research into measures recommendations protecting seabird populations. contributing mortality incidents work towards safeguarding preserving vital migration patterns.

Language: Английский

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