Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
272, P. 116028 - 116028
Published: Feb. 3, 2024
Extensive
application
of
lead
(Pb)
brought
about
environmental
pollution
and
toxic
reactions
organisms.
Selenium
(Se)
has
the
effect
antagonizing
Pb
poisoning
in
humans
animals.
However,
it
is
still
unclear
how
causes
brainstem
toxicity.
In
present
study,
we
wanted
to
investigate
whether
Se
can
alleviate
toxicity
chicken
brainstems
by
reducing
apoptosis.
One
hundred
eighty
chickens
were
randomly
divided
into
four
groups,
namely
control
group,
Se/Pb
group.
Morphological
examination,
ultrastructural
observation,
relative
mRNA
expressions
genes
on
heat
shock
proteins
(HSPs);
selenoproteins;
inflammatory
cytokines;
apoptosis-related
factors
investigated.
The
results
showed
that
exposure
led
tissue
damage
apoptosis
brainstems.
Furthermore,
an
atypical
expression
HSPs
(HSP27,
HSP40,
HSP60,
HSP70,
HSP90);
selenoprotein
family
glutathione
peroxidase
(GPx)
1,
GPx2,
GPx3,
GPx4),
thioredoxin
reductases
(Txnrd)
(Txnrd1,
Txnrd2,
Txnrd3),
dio
famliy
(diodothyronine
deiodinases
(Dio)1,
Dio2,
Dio3),
as
well
other
selenoproteins
(selenoprotein
(Sel)T,
SelK,
SelS,
SelH,
SelM,
SelU,
SelI,
SelO,
Selpb,
n1
(Sepn1),
Sepp1,
Sepx1,
Sepw1,
15-kDa
(Sep15),
selenophosphate
synthetases
2
(SPS2));
cytokines
(Interleukin
(IL-2),
IL-4,
IL-6,
IL-12β,
IL-17,
Interferon-γ
(IFN-γ));
(B-cell
lymphoma-2
(Bcl-2),
tumor
protein
53
(p53),
Bcl-2
Associated
X
(Bax),
Cytochrome
c
(Cyt
c),
Caspase-3)
identified.
An
reaction
induced
after
Pb.
alleviated
abnormal
HSPs,
selenoproteins,
cytokines,
tissues
treated
with
indicated
inflammatory,
involved
Se-resisted
poisoning.
Overall,
had
resistance
against
poisoning,
be
act
antidote
for
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(2), P. 751 - 751
Published: Jan. 17, 2025
Cadmium
(Cd)
pollution
poses
an
important
problem,
but
limited
information
is
available
about
the
toxicology
effects
of
Cd
on
freshwater
invertebrates.
We
investigated
accumulation,
oxidative
stress,
microbial
community
changes,
and
transcriptomic
alterations
in
apple
snails
(Pomacea
canaliculata)
under
stress.
The
were
exposed
to
10
μg/L
solution
for
16
days,
followed
by
a
16-day
elimination
period.
Our
results
showed
that
liver
accumulated
highest
concentration
(17.41
μg/g),
kidneys
(8.00
μg/g)
intestine-stomach
(6.68
highlighting
these
tissues
as
primary
targets
accumulation.
During
period,
concentrations
decreased
all
tissues,
with
head-foot
shell
exhibiting
over
30%
rates.
stress
also
resulted
reduced
activities
superoxide
dismutase
(SOD),
catalase
(CAT),
glutathione
transferase
(GST)
compared
control
group.
Notably,
even
after
days
depuration,
enzyme
did
not
return
normal
levels,
indicating
persistent
toxicological
effects.
exposure
significantly
diversity
gut
microbiota
P.
canaliculata.
Moreover,
transcriptome
analysis
identified
differentially
expressed
genes
(DEGs)
primarily
associated
lysosome
function,
motor
proteins,
protein
processing
endoplasmic
reticulum,
drug
metabolism
via
cytochrome
P450
(CYP450),
arachidonic
acid
metabolism,
ECM–receptor
interactions.
These
findings
suggest
predominantly
disrupts
cellular
transport
metabolic
processes.
Overall,
our
study
provides
comprehensive
insights
into
impact
canaliculata
emphasizes
importance
understanding
mechanisms
underlying
toxicity
aquatic
organisms.
Toxics,
Journal Year:
2023,
Volume and Issue:
11(9), P. 734 - 734
Published: Aug. 25, 2023
Lead
(Pb),
a
hazardous
heavy
metal,
can
damage
the
health
of
organisms.
However,
it
is
not
clear
whether
Pb
chicken
cerebellums
and
thalami.
Selenium
(Se),
an
essential
nutrient
for
organisms,
has
palliative
effect
on
poisoning
in
chickens.
In
our
experiment,
model
chickens
treated
with
Se
alone
combination
was
established
to
investigate
molecular
mechanism
alleviating
Pb-caused
both
Our
morphological
results
indicated
that
caused
apoptotic
lesions,
such
as
mitochondrial
nuclear
damage.
Further,
anti-apoptotic
gene
Bcl-2
decreased;
contrary,
four
pro-apoptotic
genes
(p53,
Bax,
Cyt
c,
Caspase-3)
increased
under
treatment,
meaning
apoptosis
via
p53-Cyt
c-Caspase-3
pathway.
Furthermore,
we
further
demonstrated
elevated
HSPs
(HSP27,
HSP40,
HSP70,
HSP90),
well
HSP70
took
part
apoptosis.
addition,
found
exposure
led
oxidative
stress
up-regulating
oxidant
H2O2
down-regulating
antioxidants
(CAT,
SOD,
GST,
GPx).
Moreover,
decreased
three
Se-containing
factors
(Txnrd1,
Txnrd2,
Txnrd3),
confirming
stress.
Interestingly,
supplementation
reversed
above
changes
by
alleviated
Pb-induced
A
time
dependency
Bcl-2,
c
cerebellums,
CAT,
GPx,
p53
thalami
Pb-exposed
HSP27
were
more
sensitive
than
those
respectively,
exposure.
severe
cerebellums.
conclusion,
after
Txnrds
mediated
stress,
up-regulated
HSPs,
finally,
triggered
antagonized
pathway
selenoproteins
This
study
provides
new
information
environmental
pollutant
detoxification
abiotic
Toxics,
Journal Year:
2023,
Volume and Issue:
11(9), P. 783 - 783
Published: Sept. 15, 2023
The
health
benefits
of
thymoquinone
(TQ)
have
been
a
significant
focus
numerous
studies.
However,
more
research
is
needed
to
ascertain
whether
its
nano-form
can
effectively
treat
or
prevent
chronic
diseases.
In
this
study,
we
investigated
how
and
nanoparticles
mitigate
liver
damage
induced
by
diazinon
in
male
Wistar
rats
explored
the
intracellular
mechanisms
involved.
Forty-two
(n
=
42)
were
randomly
allotted
into
seven
groups.
Group
1
served
as
control.
2
(vehicle)
consisted
that
received
corn
oil
via
gastric
tube
daily.
3
(TQ),
given
daily
oral
administration
TQ
(40
mg/kg
bw).
4
(thymoquinone
nanoparticles,
NTQ)
included
NTQ
(0.5
bw)
orally
for
21
days.
5
(DZN)
involved
administered
(DZN,
15
orally.
6
(TQ
+
DZN),
first
orally,
followed
DZN.
7
(NTQ
DZN)
receiving
then
After
days
treatment,
euthanized.
DZN,
enzymes
significantly
elevated
(p
<
0.05).
Additionally,
there
noticeable
increases
oxidative
injury
markers,
such
nitric
oxide,
malondialdehyde,
redox
oxygen
radicals,
overall
hydrogen
peroxide
protein
carbonyl
concentrations.
This
was
accompanied
upregulation
apoptotic
markers
(Bax,
caspase9,
caspase
3,
bax/Bcl2
ratio),
inflammatory
cytokines
(TNF-α,
IL-6),
DNA
damage.
There
also
noteworthy
decrease
0.05)
activities
antioxidant
anti-apoptotic
markers.
nanoparticle
form
mitigated
these
complications;
our
histopathological
findings
corroborated
biochemical
molecular
observations.
conclusion,
properties
thymoquinone,
form,
tandem
with
downregulation
cytokines,
provided
protective
effect
against
hepatic
dysfunction
caused
diazinon.
